Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration
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Date
2018-01-01
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor&Francis Online
Abstract
Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with
defined structure and porosity. The disadvantage of the technique is the excessive smoothness of
the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold
was combined with electrospun classic or structured nanofibers to promote cell adhesion.
Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun
layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with
unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary
chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment
showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial
chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation
will be investigated in future studies.
Description
Subject(s)
3D printing, cell infiltration, chondrocytes, electrospinning, nanofibres
Citation
ISSN
1933-6918