Browsing by Author "Prosecka Eva"

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    Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration
    (Informa UK Limited, 2018-01-01) Rampichova Michala; Kuželová Košťáková Eva; Filová Eva; Chvojka Jiří; Šafka Jiří; Pelcl Martin; Dankova J; Prosecka Eva; Buzgo Matej; Plencner Martin; Lukáš David; Amler Evžen
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    Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration
    (Taylor&Francis Online, 2018-01-01) Rampichova Michala; Kuželová Košťáková Eva; Filová Eva; Chvojka Jiří; Šafka Jiří; Pelcl Martin; Daňková, J.; Prosecka Eva; Buzgo Matej; Plencner Martin; Lukáš David; Amler Evžen
    Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.
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    The combination of nanofibrous and microfibrous materials for enhancement of cell infiltration and in vivo bone tissue formation
    (IOP Publishing, 2018-01-01) Rampichova Michala; Chvojka Jiří; Jenčová Věra; Kubíková Tereza; Tonar Zbyněk; Erben Jakub; Buzgo Matej; Dankova J; Litvinec Andrej; Vocetková K; Plencner Martin; Prosecka Eva; Sovková V; Lukášová Věra; Králíčková Milena; Lukáš David; Amler Evžen