Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration

Simple item page
dc.contributor.authorRampichova Michalacs
dc.contributor.authorKuželová Košťáková Evacs
dc.contributor.authorFilová Evacs
dc.contributor.authorChvojka Jiřícs
dc.contributor.authorŠafka Jiřícs
dc.contributor.authorPelcl Martincs
dc.contributor.authorDaňková, J.cs
dc.contributor.authorProsecka Evacs
dc.contributor.authorBuzgo Matejcs
dc.contributor.authorPlencner Martincs
dc.contributor.authorLukáš Davidcs
dc.contributor.authorAmler Evžencs
dc.date.accessioned2018-09-25T12:12:00Z
dc.date.available2018-09-25T12:12:00Z
dc.date.issued2018cs
dc.description.abstractAdditive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.en
dc.format.extent15cs
dc.identifier.WebofScienceResearcherIDH-2325-2014 Kuželová Košťáková Eva
dc.identifier.doi10.1080/19336918.2017.1385713
dc.identifier.issn1933-6918cs
dc.identifier.orcid0000-0002-5758-3206 Kuželová Košťáková Eva
dc.identifier.orcid0000-0001-8137-7878 Chvojka Jiří
dc.identifier.orcid0000-0001-6263-8016 Šafka Jiří
dc.identifier.orcid0000-0003-4037-6435 Lukáš David
dc.identifier.urihttps://dspace.tul.cz/handle/15240/30889
dc.identifier.urihttps://www.tandfonline.com/doi/pdf/10.1080/19336918.2017.1385713?needAccess=true
dc.language.isoengcs
dc.publisherTaylor&Francis Onlinecs
dc.relation.ispartofseries1cs
dc.relation.urihttp://www.tandfonline.com/doi/full/10.1080/19336918.2017.1385713cs
dc.subject3D printingcs
dc.subjectcell infiltrationcs
dc.subjectchondrocytescs
dc.subjectelectrospinningcs
dc.subjectnanofibrescs
dc.titleComposite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltrationen
local.citation.epage271-285cs
local.citation.spage271-285cs
local.identifier.publikace4342
local.relation.issue3cs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Composite 3D printed.pdf
Size:
1.77 MB
Format:
Adobe Portable Document Format
Description:
článek
Download
Collections