Browsing by Author "Rampichova Michala"

Now showing 1 - 11 of 11
  • Item
    Cell Penetration to Nanofibrous Scaffolds
    (Cell adhesion & migration, 2014-01-01) Rampichova Michala; Chvojka Jiří; Amler Evžen; Kofroňová Olga; Prosecká Eva; Buzgo Matej
  • Item
    Combination of electrospun nanofibers and surface modified 3D printing for knee cartilage tissue engineering
    (Technická univerzita v Liberci, 2015-01-01) Pelcl Martin; Košťáková Eva; Chvojka Jiří; Horáková Jana; Šafka Jiří; Kříž Kristián; Rampichova Michala; Lachman Martin
  • Item
    Combination of nanofibres and microfibres in 3D scaffold for bone or cartilage regeneration.
    (2015-01-01) Košťáková Eva; Erben Jakub; Jenčová Věra; Pilařová Kateřina; Rampichova Michala; Sanetrník Filip; Blažková Lenka; Havlíček Jiří; Novák Ondřej; Mikeš Petr; Chvojka Jiří; Lukáš David
  • Item
    Combination of Patterned Electrospun Nanofibers and 3D Printing for Knee Cartilage Tissue Engineering
    (2014-01-01) Chvojka Jiří; Šafka Jiří; Pelcl Martin; Kříž Kristián; Pilařová Kateřina; Rampichova Michala; Lachman Martin; Filová Eva; Jenčová Věra; Košťáková Eva
  • Thumbnail Image
    Item
    Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration
    (Informa UK Limited, 2018-01-01) Rampichova Michala; Kuželová Košťáková Eva; Filová Eva; Chvojka Jiří; Šafka Jiří; Pelcl Martin; Dankova J; Prosecka Eva; Buzgo Matej; Plencner Martin; Lukáš David; Amler Evžen
  • Thumbnail Image
    Item
    Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration
    (Taylor&Francis Online, 2018-01-01) Rampichova Michala; Kuželová Košťáková Eva; Filová Eva; Chvojka Jiří; Šafka Jiří; Pelcl Martin; Daňková, J.; Prosecka Eva; Buzgo Matej; Plencner Martin; Lukáš David; Amler Evžen
    Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.
  • Item
    Electrospun vascular grafts fabricated from poly(L-lactide-co-epsilon-caprolactone) used as a bypass for the rabbit carotid artery
    (IOP Publishing, 2018-01-01) Horáková Jana; Mikeš Petr; Lukáš David; Šaman Aleš; Jenčová Věra; Klápšťová Andrea; Švarcová Tereza; Ackermann Michal; Novotný Vít; Kaláb Martin; Lonský Vladimír; Bartoš Martin; Rampichova Michala; Litvinec Andrej; Kubíková Tereza; Tomášek Petr; Tonar Zbyněk
    The study involved the electrospinning of the copolymer poly(L-lactide-co-ε-caprolactone) (PLCL) into tubular grafts. The subsequent material characterization, including micro-computed tomography analysis, revealed a level of porosity of around 70%, with pore sizes of 9.34 ± 0.19 μm and fiber diameters of 5.58 ± 0.10 μm. Unlike fibrous polycaprolactone, the electrospun PLCL copolymer promoted fibroblast and endothelial cell adhesion and proliferation in vitro. Moreover, the regeneration of the vessel wall was detected following implantation and, after six months, the endothelialization of the lumen and the infiltration of arranged smooth muscle cells producing collagen was observed. However, the degradation rate was found to be accelerated in the rabbit animal model. The study was conducted under conditions that reflected the clinical requirements—the prostheses were sutured in the end-to-side fashion and the long-term end point of prosthesis healing was assessed. The regeneration of the vessel wall in terms of endothelialization, smooth cell infiltration and the presence of collagen fibers was observed after six months in vivo. A part of the grafts failed due to the rapid degradation rate of the PLCL copolymer.
  • Item
    Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits
    (MDPI AG, 2020-01-01) Filova Eva; Tonar Zbyněk; Lukášová Věra; Buzgo Matej; Litvinec Andrej; Rampichova Michala; Beznoska Jiří; Plencner Martin; Staffa Andrea; Dankova J; Soural Miroslav; Chvojka Jiří; Malečková Anna; Králíčková Milena; Amler Evžen
  • Item
    Needleless coaxial electrospinning: A novel approach to mass production of coaxial nanofibers
    (ELSEVIER SCIENCE BV, 2017-01-01) Vysloužilová Lucie; Buzgo Matej; Pokorný Pavel; Chvojka Jiří; Míčková Andrea; Rampichova Michala; Kula Jiří; Pejchar Karel; Bílek Martin; Lukáš David; Amler Evžen
  • Item
    Scaffolds Produced by Combination of 3d Printing and Electrospinning
    (2014-01-01) Chvojka Jiří; Valapková Barbora; Filová Eva; Košťáková Eva; Lachman Martin; Pelcl Martin; Šafka Jiří; Kříž Kristián; Rampichova Michala
  • Item
    The combination of nanofibrous and microfibrous materials for enhancement of cell infiltration and in vivo bone tissue formation
    (IOP Publishing, 2018-01-01) Rampichova Michala; Chvojka Jiří; Jenčová Věra; Kubíková Tereza; Tonar Zbyněk; Erben Jakub; Buzgo Matej; Dankova J; Litvinec Andrej; Vocetková K; Plencner Martin; Prosecka Eva; Sovková V; Lukášová Věra; Králíčková Milena; Lukáš David; Amler Evžen