Browsing by Author "Filová Eva"
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- ItemCombination of Patterned Electrospun Nanofibers and 3D Printing for Knee Cartilage Tissue Engineering(2014-01-01) Chvojka Jiří; Šafka Jiří; Pelcl Martin; Kříž Kristián; Pilařová Kateřina; Rampichova Michala; Lachman Martin; Filová Eva; Jenčová Věra; Košťáková Eva
- ItemComposite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration(Informa UK Limited, 2018-01-01) Rampichova Michala; Kuželová Košťáková Eva; Filová Eva; Chvojka Jiří; Šafka Jiří; Pelcl Martin; Dankova J; Prosecka Eva; Buzgo Matej; Plencner Martin; Lukáš David; Amler Evžen
- ItemComposite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration(Taylor&Francis Online, 2018-01-01) Rampichova Michala; Kuželová Košťáková Eva; Filová Eva; Chvojka Jiří; Šafka Jiří; Pelcl Martin; Daňková, J.; Prosecka Eva; Buzgo Matej; Plencner Martin; Lukáš David; Amler EvženAdditive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.
- ItemPolycaprolactone foam functionalized with chitosan microparticles– a suitable scaffold for cartilage regeneration(Institute of Physiology, Czech Academy of Sciences, 2016-01-01) Filová Eva; Jakubcová Barbora; Danilová Iveta; Košťáková Eva; Jarošíková Taťána; Chernyavskiy Oleksandr; Hejda Jan; Handl Milan; Beznoska Jiří; Nečas Alois; Rosina Jozef; Amler Evžen
- ItemScaffolds Produced by Combination of 3d Printing and Electrospinning(2014-01-01) Chvojka Jiří; Valapková Barbora; Filová Eva; Košťáková Eva; Lachman Martin; Pelcl Martin; Šafka Jiří; Kříž Kristián; Rampichova Michala
- ItemThe combination of 3D printing and nanofibers for tissue engineering of articular cartilage(TANGER spol. s r.o., 2014-01-01) Pelcl Martin; Chvojka Jiří; Košťáková Eva; Šafka Jiří; Kříž Kristián; Strnadová Kateřina; Jenčová Věra; Filová Eva