Vývoj planárních nanovlákenných vrstev pro nové aplikace v gastrointestinální chirurgii
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Použití mechanické bariéry ve formě nanovlákenné vrstvy s antiadhezivním povrchem je slibnou metodou pro prevenci vzniku nežádoucích pooperativních adhezí v gastrointestinální chirurgii. Adheze jsou závažnou komplikací, která nastává v 55-66% operací, mohou mít doživotní následky, a dokonce být i příčinou smrti pacienta. Morfologie vyvinutého materiálu byla inspirována lotosovým efektem, který se podařilo napodobit kombinací bezjehlového elektrospinningu a elektrosprayingu poly kaprolaktonu. Hydrofobicita povrchu byla dále podpořena modifikací studeným plazmatem metodou chemické depozice z plynné fáze pomocí hexamethyldisiloxanu, kdy došlo k úspěšnému snížení smáčivosti povrchu. Vytvořený materiál byl charakterizován z hlediska morfologie skenovací elektronovou mikroskopií, smáčivosti metodou přisedlé kapky a chemického složení FTIR analýzou. Tyto vlastnosti byly pozorovány po dobu šesti měsíců za účelem sledování případných změn, čímž byla ověřena stabilita úpravy. Dále byla zjištěna absorpce vrstev s destilovanou vodou a simulovanou střevní tekutinou. Cytokompatibilita byla ověřena in vitro na 3T3 myších fibroblastech dle normy ČSN EN ISO 10993-5. In vitro byla také zjišťována buněčná adheze a proliferace po 4, 7 a 14dnech pomocí MTT testu a fluorescenční mikroskopie. Adheze byla hodnocena pomocí peel testu 90° s modelem tkáně, u kterého modifikovaný materiál s hydrofobním povrchem vykazoval nižší adhezi oproti neupravenému.
Mechanical barrier as nanofibrous patch with antiadhesive surface is a promising method for preventing formation of undesirable postoperative adhesions in gastrointestinal surgery. The adhesions are a severe complication which occurs in 55-66% operations, they can cause lifelong consequences and they can potentially be the cause of patient's death. Morphology of the developed material was inspired by the lotus effect which was mimicked by combination of needless electrospinning and electrospraying technology of poly caprolactone. Surface hydrophobicity was enhanced by cold plasma modification using the chemical vapour deposition method with hexamethyldisiloxane, which led to successful decrease of surface wettability. Morphology of the fabricated material was characterised by scanning electron microscopy, wettability was obtained using the sessile drop method and chemical composition was determined by FTIR analysis. Mentioned properties were observed for a period of six months in order to track possible changes, the obtained results verified stability of the modification. Furthermore, the uptake of water and simulated intestine liquid was measured. Cytocompatibility was verified in vitro with 3T3 mouse fibroblasts according to Czech norm ČSN EN ISO 10993-5. Cell adhesion and proliferation were assessed in vitro by MTT assay and fluorescence microscope after 4, 7 and 14 days. Adhesion was evaluated by peel test 90°with model tissue, modified material with hydrophobic surface had lower adhesion in comparison with untreated one.
Mechanical barrier as nanofibrous patch with antiadhesive surface is a promising method for preventing formation of undesirable postoperative adhesions in gastrointestinal surgery. The adhesions are a severe complication which occurs in 55-66% operations, they can cause lifelong consequences and they can potentially be the cause of patient's death. Morphology of the developed material was inspired by the lotus effect which was mimicked by combination of needless electrospinning and electrospraying technology of poly caprolactone. Surface hydrophobicity was enhanced by cold plasma modification using the chemical vapour deposition method with hexamethyldisiloxane, which led to successful decrease of surface wettability. Morphology of the fabricated material was characterised by scanning electron microscopy, wettability was obtained using the sessile drop method and chemical composition was determined by FTIR analysis. Mentioned properties were observed for a period of six months in order to track possible changes, the obtained results verified stability of the modification. Furthermore, the uptake of water and simulated intestine liquid was measured. Cytocompatibility was verified in vitro with 3T3 mouse fibroblasts according to Czech norm ČSN EN ISO 10993-5. Cell adhesion and proliferation were assessed in vitro by MTT assay and fluorescence microscope after 4, 7 and 14 days. Adhesion was evaluated by peel test 90°with model tissue, modified material with hydrophobic surface had lower adhesion in comparison with untreated one.
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hydrofobní povrch, antiadhezivní úprava, pooperativní adheze, střevní anastomóza, elektrospraying, lotosový efekt, tkáňové inženýrství, hydrophobic surface, antiadhesive treatment, postoperative adhesion, intestinal anastomoses, electrospraying, lotus effect, tissue engineering