Využití nanovlákenných nosičů biomasy při biofiltraci kontaminovaných vzdušnin
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Tato práce se zaměřuje na uplatnění nanovláken jako nosičů biomasy při biofiltraci znečištěného vzduchu, kde byl jako polutant použit plynný toluen. V první části práce je zpracována literární rešerše na téma biofiltrace, bioreaktory a faktory ovlivňující provoz biofiltrační jednotky. Dále je v tomto oddíle popsáno využití nanovláken ve filtraci vzduchu a jejich výroba pomocí elektrostatického zvlákňování. Na základě rešerše bylo navrženo schéma biofiltrační jednotky a byly zvoleny její komponenty a metody její analýzy. Byly vyrobeny nosiče biomasy z polyuretanových nanovláken. Po sestavení biofiltrační jednotky proběhla prostřednictvím cirkulačního okruhu inokulace kolony konsorciem, které obsahovalo především bakterie rodu Rhodococcus. Vývoj biofilmu na nosičích byl sledován mikroskopickým snímáním. Po dostatečném nárůstu biofilmu byla zavedena do biofiltrační jednotky směs syntetického vzduchu a toluenu z tlakových láhví.
This thesis focuses on the application of nanofibers as carriers of biomass for biofiltration of polluted air, where the pollutant is gaseous toluene. The first part of this thesis deals with general aspects of biofiltration, bioreactors and factors influencing the operation of biofilter. In this section the application of nanofibers for filtration and their production by electrospinning are also described. Based on the literature research, the scheme of biofilter was designed and its components were chosen. The analytical methods for biofilter operation were selected. The biomass carriers were made of polyurethane nanofibers. After assembling biofilter the column was inoculated through the circulation circuit by consortium containing bacteria of the genus Rhodococcus. The biofilm development was observed by microscopic techniques. After sufficient growth of biofilm on the carriers, the mixture of synthetic air and toluene from gas cylinders was fed into biofilter.
This thesis focuses on the application of nanofibers as carriers of biomass for biofiltration of polluted air, where the pollutant is gaseous toluene. The first part of this thesis deals with general aspects of biofiltration, bioreactors and factors influencing the operation of biofilter. In this section the application of nanofibers for filtration and their production by electrospinning are also described. Based on the literature research, the scheme of biofilter was designed and its components were chosen. The analytical methods for biofilter operation were selected. The biomass carriers were made of polyurethane nanofibers. After assembling biofilter the column was inoculated through the circulation circuit by consortium containing bacteria of the genus Rhodococcus. The biofilm development was observed by microscopic techniques. After sufficient growth of biofilm on the carriers, the mixture of synthetic air and toluene from gas cylinders was fed into biofilter.
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biofiltrace, biofiltr, nanovlákna, nosiče biomasy, Rhodococcus, toluen, biofiltration, biofilter, nanofibers, biomass carriers, Rhodococcus, toluene