Vývoj mikrovláknených buněčných nosičů pomocí metody Rapid Prototyping
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Date
2019-05-20
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Abstract
Bakalarska prace se zabyva vyvojem unikatni struktury bunecneho nosice pomoci technologie FDM/FFM 3D tisku a naslednym biologickym testovanim s lidskymi bunkami v podobe in vitro testu. Cilem prace je vytvoreni takove struktury a jejiho fyzickeho modelu, ktery se v ramci tkanoveho inzenyrstvi s umyslem nahrady nefunkcni kostni, svalove ci chrupavcite tkane bude dal vyvijet az do stavu, kdy bude moznost takovyto scaffold otestovat in vivo metodami. Testovanymi materialy byly poly(- kaprolakton) a kyselina polymlecna. Oba materialy jsou v oboru tkanoveho inzenyrstvi siroce vyuzivany. Vysledkem prace bylo zjisteni, ze z kyseliny polymlecne se jednoznacne lepe tisklo a vysledne nosice byly jednotnejsi. Za nastavenych podminek biologickeho testovani vykazovala kyselina polymlecna taktez vyssi miru poctu a proliferace bunek MG-63, coz bylo dokazano MTT testem.
Bachelor thesis deals with development of a scaffold's unique structure using the FDM/FFM 3D printing as one of the methods of Rapid Prototyping and biological testing afterwards using the in vitro lab methods. The aim of this pursue is to develop such a structure and its physical model which is intended to replace dysfunctional bone, muscular or cartilage structures, that has certain properties and hence is suitable foradvanced in vivo testing. Materials used within this experiment were poly(- caprolactone) and polylactid acid. Bothmaterials are respected and widely used in the field of tissue engineering. The results of this work bring to the attention that printing the scaffolds using the polylactid acid was much easier and that such printed scaffolds manifested more of an equal physical appearance. The conditions set for biological testing also suited the polylactid acid better as these scaffolds showed higher rate of thriving MG-63 cells during the MTT testing.
Bachelor thesis deals with development of a scaffold's unique structure using the FDM/FFM 3D printing as one of the methods of Rapid Prototyping and biological testing afterwards using the in vitro lab methods. The aim of this pursue is to develop such a structure and its physical model which is intended to replace dysfunctional bone, muscular or cartilage structures, that has certain properties and hence is suitable foradvanced in vivo testing. Materials used within this experiment were poly(- caprolactone) and polylactid acid. Bothmaterials are respected and widely used in the field of tissue engineering. The results of this work bring to the attention that printing the scaffolds using the polylactid acid was much easier and that such printed scaffolds manifested more of an equal physical appearance. The conditions set for biological testing also suited the polylactid acid better as these scaffolds showed higher rate of thriving MG-63 cells during the MTT testing.
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nanovlakna, 3D tisk, tkanove inzenyrstvi, bunecny nosic, nanofibers, 3D printing, tissue engineering, scaffolds