Browsing by Author "Jenčová, Věra"

Now showing 1 - 8 of 8
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    A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane
    (Dovepress, 2019-07-08) Bačáková, Markéta; Pajorova, Julia; Brož, Antonín; Hadraba, Daniel; Lopot, František; Zavaďáková, Anna; Vištejnová, Lucie; Beno, Milan; Kostič, Ivan; Jenčová, Věra; Bačáková, Lucie
    Background: Repairs to deep skin wounds continue to be a difficult issue in clinical practice. A promising approach is to fabricate full-thickness skin substitutes with functions closely similar to those of the natural tissue. For many years, a three-dimensional (3D) collagen hydrogel has been considered to provide a physiological 3D environment for co-cultivation of skin fibroblasts and keratinocytes. This collagen hydrogel is frequently used for fabricating tissue-engineered skin analogues with fibroblasts embedded inside the hydrogel and keratinocytes cultivated on its surface. Despite its unique biological properties, the collagen hydrogel has insufficient stiffness, with a tendency to collapse under the traction forces generated by the embedded cells. Methods: The aim of our study was to develop a two-layer skin construct consisting of a collagen hydrogel reinforced by a nanofibrous poly-L-lactide (PLLA) membrane pre-seeded with fibroblasts. The attractiveness of the membrane for dermal fibroblasts was enhanced by coating it with a thin nanofibrous fibrin mesh. Results: The fibrin mesh promoted the adhesion, proliferation and migration of the fibroblasts upwards into the collagen hydrogel. Moreover, the fibroblasts spontaneously migrating into the collagen hydrogel showed a lower tendency to contract and shrink the hydrogel by their traction forces. The surface of the collagen was seeded with human dermal keratinocytes. The keratinocytes were able to form a basal layer of highly mitotically-active cells, and a suprabasal layer. Conclusion: The two-layer skin construct based on collagen hydrogel with spontaneously immigrated fibroblasts and reinforced by a fibrin-coated nanofibrous membrane seems to be promising for the construction of full-thickness skin substitute.
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    Biofyzika I
    (2020-01-01) Lukáš, David; Trčka, Michal; Kuželová Košťáková, Eva; Jenčová, Věra; Asatiani, Nikifor
    Cílem projektu je vytvoření učebního textu (skript) k novému předmětu Biofyzika v rámci nově akreditovaného SP Bioinženýrství.
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    The combination of meltblown technology and electrospinning - The influence of the ratio of micro and nanofibers on cell viability
    (Elsevier, 2016-01-01) Erben, Jakub; Jenčová, Věra; Chvojka, Jiří; Blažková, Lenka; Strnadová, Kateřina; Modrak, Miroslav, Košťáková Kuželová Eva
    This study describes the production, testing and characterization of biodegradable scaffolds for bone tissue, which consist of the exact ratio of meltblown microfibers and nanofibers produced through the electrostatic field. All fibrous materials were produced from polycaprolactone. Three kinds of materials were prepared in the experiment with the same area density and with different well-defined ratio of microfibers as a mechanical component and nanofibers as a cells adherent component. All prepared materials showed optimum porosity of the inner structure for cell proliferation and in comparison to the materials with nanofibers they had good mechanical properties. Important structural properties and homogenity of each material were observed by electron microscopy and analyzed by image analysis. The effect of various ratios of microfibers and nanofibers on adhesion and proliferation of osteoblasts in-vitro was characterized. © 2016 Elsevier B.V. All rights reserved.
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    Cyclodextrin-Polypyrrole Coatings of Scaffolds for Tissue Engineering
    (2019-03-11) Lukášek, Jan; Hauzerová, Šárka; Havlíčková, Kristýna; Strnadová, Kateřina; Mašek, Karel; Stuchlík, Martin; Stibor, Ivan; Jenčová, Věra; Řezanka, Michal
    Polypyrrole is one of the most investigated conductive polymers used for tissue engineering applications because of its advantageous properties and the ability to promote different cell types’ adhesion and proliferation. Together with β-cyclodextrin, which is capable of accommodating helpful biomolecules in its cavity, it would make a perfect couple for use as a scaffold for tissue engineering. Such scaffolds were prepared by the polymerisation of 6-(pyrrol-3-yl)hexanoic acid on polycaprolactone microfibres with subsequent attachment of β-cyclodextrin on the polypyrrole layer. The materials were deeply characterised by several physical and spectroscopic techniques. Testing of the cyclodextrin enriched composite scaffold revealed its better performance in in vitro experiments compared with pristine polycaprolactone or polypyrrole covered polycaprolactone scaffolds.
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    Nanovlákna. Teorie, technologie a použití
    (2020-01-01) Lukáš, David; Kuželová Košťáková, Eva; Jenčová, Věra
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    Novel chapter in hybrid materials: One-pot synthesis of purely organosilane fibers
    (Elsevier Ltd, 2020-03-01) Holubová, Barbora; Máková, Veronika; Müllerová, Jana; Brus, Jiří; Havlíčková, Kristýna; Jenčová, Věra; Michalcová, Alena; Kulhánková, Johana; Řezanka, Michal
    Despite the extensive literature on organosilane hybrid materials, there are very few reports on the preparation of purely organosilane non-woven fibrous mats. Nevertheless, their use in various application fields may be of great importance regarding the global issue of polymer fibers and the toxicity of other compounds used so far in the composite polymer-organosilane fiber-manufacturing process. The greatest obstacle seems to be the supposed difficulty in the polymerization of organosilanes in a spinnable polymeric solution. In this work, we report the one-pot synthesis of electrospun organosilane fibers without any kinds of surfactants, low-molecular-weight polymeric gelators or spinnable polymers. The purely organosilane fibrous mats were prepared only via a suitable adjustment of the sol-gel processing parameters and were successfully produced an industrial electrospinning device, promising possible fabrication on a large scale. Moreover, the use of two differently-structured model benzene functionalized organoalkoxysilanes proves the possibility of designing a molecularly engineered material tailored to specific applications. These organosilane fibrous mats seem to be promising in various application fields, such as reusable catalysts, adsorption or conducting membranes or as novel biomaterials. This latter of these areas was preliminarily studied herein.
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    Novel double-layered planar scaffold combining electrospun PCL fibers and PVA hydrogels with high shape integrity and water stability
    (Elsevier B.V., 2020-03-01) Klíčová, Markéta; Klápšťová, Andrea; Chvojka, Jiří; Kopřivová, Barbora; Jenčová, Věra; Horáková, Jana
    Novel double-layered materials with different properties of each side were prepared via needleless electrospinning and compared in terms of morphology, wettability, adhesion and proliferation of mouse fibroblasts. The materials consist of hydrophilic poly(vinyl alcohol) fibers with low (PVA_L) or high (PVA_H) degree of hydrolysis, and hydrophobic poly(ε-caprolactone) (PCL) fibrous layer. Although the PVA_L fibers were fully dissolved following a water exposure, the shape of the scaffold was maintained due to water stable PCL layer. Exposing PVA_H based fibrous layer to water created a hydrogel-like structure with shape defined by the PCL layer. According to the MTT assay, the mouse fibroblasts seeded on the scaffold exhibited the greatest proliferative activity on the PCL fibers. These double-layered scaffolds with different features on each side are very promising for many novel medical applications such as wound dressing or abdominal adhesion prevention.
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    Podpora proliferace fibroblastů pomocí trombocytů inkorporovaných do 3D nanovlákenných vrstev
    (Technická Univerzita v Liberci, 2014-01-01) Zdrálková, Kateřina; Jenčová, Věra
    Tato diplomová práce se zabývá problematikou proliferace fibroblastů do vnitřních prostor 3D nanovlákenných vrstev (tloušťky>300m). Studium inkorporace trombocytárních růstových faktorů do nanovlákenných biodegradabilních scaffoldů je z hlediska léčebných aplikací přínosné. [3],[4],[7],[8]. Tyto 3D biodegradabilní scaffoldy ze syntetických polymerů mohou být v budoucnu použity pro náhrady některých tkání. V současné době probíhají v řadě laboratoří testy in vivo i testy in vitro na těchto materiálech.[8] V mnoha podobných případech buňky proliferují pouze do omezené tloušťky materiálu. Využití trombocytárních přípravků (např. PRP, PL) a především růstových faktorů v nich, má z hlediska proliferace strukturou velmi pozitivní výsledky v mnoha studiích. [7],[9] Trombocytární přípravky mohou být autologní, což je ve spojení s využitím biokompatibilního scaffoldu výhodné z hlediska zmenšení imunologických rizik. Oproti tomu například dodnes stále používané alogenní či xenogenní náhrady tkání tyto rizika představují.