Browsing by Author "Chvojka, Jiří"
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- Item3D-Printed Magnetic Stirring Cages for Semidispersive Extraction of Bisphenols from Water Using Polymer Micro- and Nanofibers(AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2020-03-03) Šrámková, Ivana; Horstkotte, Burkhard; Erben, Jakub; Chvojka, Jiří; Švec, František; Solich, Petr; Satinský, DaliborA magnetic stirring device allowing semidispersive solid phase extraction of eight bisphenols (A, AF, AP, C, BP, G, M, and Z) from river waters using polymer nano- and microfibers followed by HPLC with spectrophotometric detection has been developed and applied. About 50 mg of fibers was placed in a round, cage-like housing consisting of two identical 3D printed pieces that were locked together by a magnetic stirring bar. Magnetic stirring action of the cage devices enabled highly efficient interaction of the fibers housed inside with the aqueous samples and analyte transfer without risking fiber compaction and/or damaging. Polypropylene was found to be the best-suited filament material for the cage 3D printing, and polycaprolactone fibers appeared the most efficient sorbent out of eight tested polymers. Experimental design revealed that analytes extraction from 100 mL aqueous samples was completed within 50 min and stripping in methanol required less than 35 min. Cage housing enabled simple and robust handling of the fibrous sorbent that could be used repeatedly up to at least 5 times. Procedural repeatability was less than 5% RSD, and limits of detection and quantitation were 0.1-2.1 and 0.4-7.0 mu g L-1, respectively. Analyte recoveries at 50 mu g L-1 level ranged from 87.1% to 106.5% in the analysis of two spiked river and two lake waters.
- Itemac Bubble Electrospinning Technology for Preparation of Nanofibrous Mats(AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2020-04-14) Erben, Jakub; Kalous, Tomáš; Chvojka, JiříThis study deals with a new combination of alternating current (ac) electrospinning and bubble electrospinning. Research devoted to the combination of these two methods for the preparation of nanofibrous and microfibrous mats has been carried out. The design, construction, and description of bubble electrospinning are described in this article. The final morphologies of the fibrous layers produced by these methods have been compared with other well-known electrospinning methods. The bubble electrospinning and ac electrospinning aspire to become new technologies that could be utilized in various technical areas and tissue-engineering applications.
- ItemThe combination of meltblown technology and electrospinning - The influence of the ratio of micro and nanofibers on cell viability(Elsevier, 2016-01-01) Erben, Jakub; Jenčová, Věra; Chvojka, Jiří; Blažková, Lenka; Strnadová, Kateřina; Modrak, Miroslav, Košťáková Kuželová EvaThis study describes the production, testing and characterization of biodegradable scaffolds for bone tissue, which consist of the exact ratio of meltblown microfibers and nanofibers produced through the electrostatic field. All fibrous materials were produced from polycaprolactone. Three kinds of materials were prepared in the experiment with the same area density and with different well-defined ratio of microfibers as a mechanical component and nanofibers as a cells adherent component. All prepared materials showed optimum porosity of the inner structure for cell proliferation and in comparison to the materials with nanofibers they had good mechanical properties. Important structural properties and homogenity of each material were observed by electron microscopy and analyzed by image analysis. The effect of various ratios of microfibers and nanofibers on adhesion and proliferation of osteoblasts in-vitro was characterized. © 2016 Elsevier B.V. All rights reserved.
- ItemElektrické zvlákňování: koncept výroby nanovláken a mikrovláken, možné aplikace vlákenných materiálů(2021-06-29) Chvojka, Jiří; Technická univerzita v LiberciHabilitační práce je předložena ve formě komentovaného souboru prací dle Zákona č. 111/1998 Sb. Publikované výsledky přibližují význačný svět nanovláken a mikrovláken, zároveň také samotné technologie vedoucí k jejich přípravě. Habilitační práce je sepsána jako komentovaný soubor sebraných vědeckých prací. Jedná se o soubor impaktovaných článků, které jsou zaznamenány v databázích WoS nebo Scopus. Všechny publikované články jsou podpořeny uveřejněnými patenty, užitnými vzory, nebo přijatými patentovými přihláškami. Propojení všech těchto vědeckých a inženýrských prací může být důkazem, že aplikace nanovlákenných i mikrovlákenných materiálů směřují do průmyslové praxe. Dle zákona č. 111/1998 Sb je nutné uvádět také výstupy vědecko-pedagogické činnosti. Kvantifikace výsledků VaV bude uvedena v přehledné souhrnné tabulce v kapitole soubor vědeckých prací. Habilitační práce je orientována na textilní technologie výroby vlákenných i nanovlákenných materiálů vhodných pro zdravotnické i technické aplikace. V práci jsou uveřejněny výsledky technického i biologického zaměření. Originalita práce spočívá zejména v propojení vědeckých publikací přímo s uveřejněnými patenty nebo užitnými vzory. V současné době je cíleno na možnou komercionalizaci vědeckých výstupů ve formě spolupráce s výrobním subjektem. V práci je uvedena přímá návaznost vědeckých článků a jejich propojení s průmyslovou sférou. Přínosem podaných výsledků je zejména novost vytvořených nanovlákenných materiálů a jejich následný aplikační potenciál. Příkladem může být nanovlákenný kožní kryt pro léčbu kožních poranění, který je ve fázi klinického testování. Pedagogická činnost zahrnuje výuku dvou předmětů, Zdravotnické textilie a předmětu Termické a chemické technologie.
- ItemFabrication of dual-functional composite yarns with a nanofibrous envelope using high throughput AC needleless and collectorless electrospinning(NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND, 2019-02-12) Valtera, Jan; Kalous, Tomáš; Pokorný, Pavel; Baťka, Ondřej; Bílek, Martin; Chvojka, Jiří; Mikeš, Petr; Kuželová Košťáková, Eva; Žabka, Petr; Ornstová, Jana; Beran, Jaroslav; Stanishevsky, Andrei; Lukáš, DavidNanotechnologies allow the production of yarns containing nanofibres for use in composites, membranes and biomedical materials. Composite yarns with a conventional thread core for mechanical strength and a nanofibrous envelope for functionality, e.g. biological, catalytic, have many advantages. Until now, the production of such yarns has been technologically difficult. Here, we show an approach to composite yarn production whereby a plume of nanofibers generated by high throughput AC needleless and collectorless electrospinning is wound around a classic thread. In the resulting yarn, nanofibres can form up to 80% of its weight. Our yarn production speed was 10 m/min; testing showed this can be increased to 60 m/min. After the yarn was embedded into knitwear, scanning electron microscope images revealed an intact nanofibrous envelope of the composite yarn. Our results indicate that this production method could lead to the widespread production and use of composite nanofibrous yarns on an industrial scale.
- ItemHydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits(MDPI, 2020-01-01) Filová, Eva; Tonar, Zbyněk; Lukášová, Věra; Buzgo, Matěj; Litvinec, Andrej; Rampichová, Michala; Beznoska, Jiří; Plecner, Martin; Staffa, Andrea; Daňková, Jana; Soural, Miroslav; Chvojka, Jiří; Malečková, Anna; Králíčková, Milena; Amler, EvženHydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested; fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µm) and further modified by polyethylene glycol–biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation.
- ItemImpact of Various Sterilization and Disinfection Techniques on Electrospun Poly-epsilon-caprolactone(AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2020-04-21) Horáková, Jana; Klíčová, Markéta; Erben, Jakub; Klápšťová, Andrea; Novotný, Vít; Běhálek, Luboš; Chvojka, JiříElectrospun materials made from biodegradable polycaprolactone are used widely in various tissue engineering and regenerative medicine applications because of their morphological similarity to the extracellular matrix. However, the main prerequisite for the use of such materials in clinical practice consists of the selection of the appropriate sterilization technique. This study is devoted to the study of the impact of traditional sterilization and disinfection methods on a nanofibrous polycaprolactone layer constructed by means of the needleless electrospinning technique. It was determined that hydrogen peroxide plasma treatment led to the loss of fibrous morphology and the creation of a foil. However, certain sterilization (ethylene oxide, gamma irradiation, and peracetic acid) and disinfection techniques (ethanol and UV irradiation) were found not to lead to a change in morphology; thus, the study investigates their impact on thermal properties, molecular weight, and interactions with a fibroblast cell line. It was determined that the surface properties that guide cell adhesion and proliferation were affected more than the bulk properties. The highest proliferation rate of fibroblasts seeded on nanofibrous scaffolds was observed with respect to gamma-irradiated polycaprolactone, while the lowest proliferation rate was observed following ethylene oxide sterilization.
- ItemImpact of Various Sterilization and Disinfection Techniques on Electrospun Poly-ϵ-caprolactone(American Chemical Society, 2020-01-01) Horáková, Jana; Klíčová, Markéta; Erben, Jakub; Klápšťová, Andrea; Novotný, Vít; Běhálek, Luboš; Chvojka, JiříElectrospun materials made from biodegradable polycaprolactone are used widely in various tissue engineering and regenerative medicine applications because of their morphological similarity to the extracellular matrix. However, the main prerequisite for the use of such materials in clinical practice consists of the selection of the appropriate sterilization technique. This study is devoted to the study of the impact of traditional sterilization and disinfection methods on a nanofibrous polycaprolactone layer constructed by means of the needleless electrospinning technique. It was determined that hydrogen peroxide plasma treatment led to the loss of fibrous morphology and the creation of a foil. However, certain sterilization (ethylene oxide, gamma irradiation, and peracetic acid) and disinfection techniques (ethanol and UV irradiation) were found not to lead to a change in morphology; thus, the study investigates their impact on thermal properties, molecular weight, and interactions with a fibroblast cell line. It was determined that the surface properties that guide cell adhesion and proliferation were affected more than the bulk properties. The highest proliferation rate of fibroblasts seeded on nanofibrous scaffolds was observed with respect to gamma-irradiated polycaprolactone, while the lowest proliferation rate was observed following ethylene oxide sterilization.
- ItemImproving dielectric properties and suppression of partial discharges in fiber/thermoset-matrix composites by polymeric nanofibers(Institute of Electrical and Electronics Engineers Inc., 2019-01-01) Polanský, Radek; Prosr, Pavel; Pihera, Josef; Chvojka, Jiří; Kyselák, TomášThe polymeric nanofibrous layers as a new material for possible improving dielectric properties and suppression of partial discharges in fiber/thermoset-matrix composites are introduced in this paper. Electrospun nanofibers made from polybenzimidazole (PBI) and polyimide (PI) were incorporated into the structure of the commonly used fiber/thermoset-matrix composites to enhance their dielectric behavior. PBI and PI were electrospun using a Nanospider laboratory scale machine. The spinning process was set to produce nanofibrous layers with two different areal weights (1 and 3 g.m-2 for composites with PBI and 3 and 5 g.m-2 for composites with PI). Control composites without nanofibers as well as composites containing electrospun PBI and PI nanofibrous layers were manufactured by compression molding in a laboratory press without any previous vacuum debulking. To verify the positive or negative influence of the incorporated nanofibrous layers on the overall dielectric behavior of the composites, the volume resistivity p (Ωm) and dielectric strength Ed(kV.mm-1) were comprehensively measured. Initial results revealed that the volume resistivity of modified composites increased (of about 126 % for PBI and 217 % for PI) as well as the dielectric strength (of about 11 % for PBI and 53 % for PI). Obtained results were subsequently supported by partial discharge analysis which confirmed that the nanofibrous layers are capable to significantly suppress the partial discharge activity inside the composite structure.
- ItemKombinace 3D tisku a nanovláken pro tkáňové inženýrství kloubní chrupavky(Technická Univerzita v Liberci, 2015-01-01) Pelcl, Martin; Chvojka, JiříTato práce se zabývá studiem kombinace metody 3D tisku s nanovlákny pro tkáňové inženýrství kloubní chrupavky vývoj tkáňového nosiče pro nahrazení poškozené chrupavčité tkáně. První část práce je věnována rešerši, která byla vypracována na dané téma a z níž bylo vycházeno dále při tvorbě práce. Teoretická část je zaměřena na biologii hyalinní chrupavky, proces tkáňového inženýrství a dále zde jsou konkrétně představeny přístroje, materiály a postupy, které byly využity v experimentální části. V experimentální části je v časové posloupnosti popsán postup výroby tkáňového nosiče a jeho testování. Jsou zde uvedeny dosažené výsledky, které jsou dále rozebrány a vyhodnoceny.
- ItemKontrolované ukládání nanovlákených vrstev za použití metody elektrostatického zvlákňování(Technická Univerzita v Liberci, 2013-01-01) Pankrác, Jakub; Chvojka, JiříVybrané téma bakalářské práce se zabývá zpracováním rešerše v oblasti fokusace nanovláken a sestrojením laboratorního zařízení, pomocí kterého bude možné usměrnit nanovlákenou vrstvu pomocí elektrostatické čočky. Následně podrobit fokusovanou a nefokusovanou vrstvu mikroskopové analýze. Hlavním účelem fokusace je zamezit ztrátám nanovláken při samotném procesu elektrostatického zvlákňování, kdy zejména polymerní materiály využívající se v medicínském průmyslu jsou finančně nákladné.
- ItemNovel double-layered planar scaffold combining electrospun PCL fibers and PVA hydrogels with high shape integrity and water stability(Elsevier B.V., 2020-03-01) Klíčová, Markéta; Klápšťová, Andrea; Chvojka, Jiří; Kopřivová, Barbora; Jenčová, Věra; Horáková, JanaNovel double-layered materials with different properties of each side were prepared via needleless electrospinning and compared in terms of morphology, wettability, adhesion and proliferation of mouse fibroblasts. The materials consist of hydrophilic poly(vinyl alcohol) fibers with low (PVA_L) or high (PVA_H) degree of hydrolysis, and hydrophobic poly(ε-caprolactone) (PCL) fibrous layer. Although the PVA_L fibers were fully dissolved following a water exposure, the shape of the scaffold was maintained due to water stable PCL layer. Exposing PVA_H based fibrous layer to water created a hydrogel-like structure with shape defined by the PCL layer. According to the MTT assay, the mouse fibroblasts seeded on the scaffold exhibited the greatest proliferative activity on the PCL fibers. These double-layered scaffolds with different features on each side are very promising for many novel medical applications such as wound dressing or abdominal adhesion prevention.
- ItemNovel nanofibrous sorbents for the extraction and determination of resveratrol in wine(2019-07-26) Háková, Martina; Chocholoušová Havlíková, Lucie; Švec, František; Solich, Petr; Erben, Jakub; Chvojka, Jiří; Šatínský, DaliborOn-line SPE HPLC method using nanofibrous sorbents for the extraction and determination of resveratrol in wine was developed and validated. Different types of nanofibrous and microfibrous polymers were tested and compared with commercial monolithic C18 sorbent. Polyamide and polyacrylonitrile nanofibers and composite materials composed of respective polycaprolactone and poly(vinylidene difluoride) nanofibers at microfibrous scaffold were included among tested materials. Two different polycaprolactone-based materials were prepared and their effect on the extraction properties studied. Alternatively, dopamine-coated polycaprolactone fibers were also used. Poly(vinylidene difluoride) nanofibers/polycaprolactone microfibers composite was found as the most effective sorbent and utilized for the method validation. Resveratrol in red wine was determined using our validated on-line SPE HPLC method.
- ItemPartial discharges of nonwoven nanofibers composite(University of West Bohemia, Faculty of Electrical Engineering, Regional Innovation Centre for Electrical Engineering, Univerzitní 8, Pilsen, Czech Republic; Technical University of Liberec, Department of Machinery Construction, Laboratory of Nanofiber and Nanosurface Preparation, Studentská 1402/2, Liberec, Czech Republic, 2016-01-01) Pihera, Josef; Polanský, Radek; Zemanová, Monika; Prosr, Pavel; Chvojka, JiříA promising technology of nanofibrous composites is studied nowadays as an alternative method to the well-known dielectric nanocomposites filled by various nanofillers like a metallic oxides, alumina, silica, carbon nanofibers or nanotubes. All of these nanofillers are known, more or less. The use is to improve some of the electrical, mechanical and thermal properties of nanocomposites. Unfortunately, its expensiveness and tendency to agglomeration remain as their main disadvantage. In the contrary, the nanofibers can be applied as a nonwoven fabric over the surface of the composite with no tendency to create the cluster agglomeration as the nanocomposites with nanoparticles. It was prepared experimental specimens of nonwoven nanofibers composites based on the modification of commonly used three-component mica-based electrical insulating material (epoxy, glass fibers and mica). The modification of these, well known, mica composites was done by incorporation of the nonwoven nanofibers layers (1, 2 and 3) to its structure always with different area density (1, 3 and 5 g/m2) of the nanofibers. The tested material was delivered in the form of resin-rich sheets on which the layers of the nanofibers made from Polyamide 6 were applied and specimens were subsequently cured using typical resin rich curing process. The influence of prepared modifications on the partial discharge characteristics and magnitudes of the resulting nanocomposites was studied. Partial discharges results show differences between the specimens' variations depending on nanofibers presence, the number of layers and surface density of nanofibers. The decreasing of the partial discharge activity is recognisable when the nanofabrics is incorporated into the composite. The obtained results proved that the nonwoven nanofibers based on Polyamide 6 seem to be a perspective material with certain resistance to partial discharge activity. © 2016 IEEE.
- ItemPoly-epsilon-caprolactone Nanofibrous Polymers: A Simple Alternative to Restricted Access Media for Extraction of Small Molecules from Biological Matrixes(AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2020-05-19) Raabová, Hedvika; Háková, Martina; Chocholoušová Havlíková, Lucie; Erben, Jakub; Chvojka, Jiří; Solich, Petr; Švec, František; Šatínský, DaliborPoly-epsilon-caprolactone nanofibrous polymer has been used as an alternative to restricted access media for extraction of protein-containing biological samples and direct transfer in the chromatographic system. Three commercial cartridges differing in length and internal diameter have been manually packed with the composite material prepared from poly-epsilon-caprolactone nanofibers coated on poly-epsilon-caprolactone microfibrous scaffold and connected to the column-switching chromatographic system. Bovine milk and human serum (25 mu L) spiked with a mixture of methyl-, ethyl-, propyl-, and butylparaben in a concentration range of 1-100 mu g mL(-1) were online extracted using the cartridge-containing fibers. Then, 5 and 20% (v/v) aqueous methanol was applied as the washing mobile phase. While the ballast protein macromolecules were quantitatively eluted from the nano/microfibrous composite sorbent, the parabens were retained. After the mobile phase was switched to a stronger one, these compounds were then eluted from the extraction sorbent, directed in the analytical column, and finally separated. An extraction efficiency of 86-101% for all parabens achieved using the optimum-sized cartridge and a repeatability of the extraction procedure of 0.06-1.95% RSD were obtained.
- ItemPolycaprolactone nanofibers functionalized with a dopamine coating for on-line solid phase extraction of bisphenols, betablockers, nonsteroidal drugs, and phenolic acids(Springer-Verlag Wien, 2019-11-01) Háková, Martina; Chocholoušová Havlíková, Lucie; Chvojka, Jiří; Erben, Jakub; Solich, Petr; Švec, František; Šatínský, DaliborPolycaprolactone composite nanofibers coated with a polydopamine layer are introduced as a new type of absorption material for on-line solid phase extraction (SPE) in chromatographic system. A hybrid technology combining the electrospinning and melt blowing was used for the preparation of 3D-structured microfiber/nanofibrous polycaprolactone composite. The dopamine coating was then applied to functionalize the micro/nanofibers. Polydopamine-coated polycaprolactone fibers were tested as an extraction phase in on-line SPE prior to HPLC separation and UV detection. Four groups of biologically active substances including bisphenols (Bisphenol S, Bisphenol AF, Bisphenol A, Bisphenol C, Bisphenol AP, Bisphenol Z, Bisphenol BP, and Bisphenol M), betablockers (Timolol, Metoprolol, Labetalol, and Propranolol), nonsteroidal antiphlogistic drugs (Salicylic acid, Ketoprofen, Naproxen, Indomethacin, Diclofenac, Ibuprophen, and Meclofenamic acid), and phenolic acids (Chlorogenic acid, Caffeic acid, Sinapic acid, m-Coumaric acid, Benzoic acid, and Cinnamic acid) were used as the model analytes. Neat and coated fibers were compared and applied as sorbents for the on-line extraction set-up. Both materials produced good extraction potential for the determination of bisphenols and nonsteroidal drugs in model biological and environmental samples including river water, human urine, and blood serum. However, the polydopamine layer significantly increased the extraction efficiency of polar drugs. Typical repeatability of on-line extraction procedure on polydopamine coated fibers was in the range 0.12–4.11% for bisphenols, 0.55–1.41% for antiphlogistic drugs, 0.59–2.52% for phenolic acids, and 1.01–1.65% for betablockers.
- ItemProduction of gelatin nanofibrous layers via alternating currentelectrospinning(Elsevier, 2019-05-29) Jirkovec, Radek; Kalous, Tomáš; Brayer, W. Anthony; Stanishevky, Andrei V.; Chvojka, JiříGelatin is a biocompatible polymer that is widely used in tissue engineering. Nowadays, nanofibrous scaffolds are produced mainly by the direct voltage spinning method which, however, does not currently have the same production capacity as the newly developed alternating current (AC) spinning technology. Using AC spinning, nanofibrous layers made from a 20% concentration of gelatin in a solvent system containing acetic acid, distilled water, and ethanol were spun. A solution containing minimal acetic acid which could still be spun optimally was searched for spinning. The produced gelatin nanofibrous layers were crosslinked and, under various conditions, subjected to mechanical testing.
- ItemProduction of Poly(vinylalcohol) Nanoyarns Using a Special Saw-like Collector(Inst Chemical Fibres, 2013-01-01) Chvojka, Jiří; Hinestroza, J. P.; Lukáš, DavidThis work introduces an electrospinning method for laboratory-scale production of nanofibrous materials from polyvinylalcohol (PVA) nanofibres. A procedure for the subsequent production of twisted yarns from the aligned nanofibrous strand is introduced as well. Both needle and needleless electrospinning variants were employed Mechanical properties of the nanoyarns produced were tested using a VIBRODYN 400 and their morphology was investigated by light and electron microscopy. The work also introduces a simple analysis of the field strength that causes the prevailing unidirectional fiber deposition between neighbouring lamellae of a special saw-like collector The field strength analysis was carried out both analytically and by modelling based on the software COMSOL Multiphysics.
- ItemScreening of extraction properties of nanofibers in a sequential injection analysis system using a 3D printed device(ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, 2019-05-15) Šrámková, Ivana H.; Carbonell-Rozas, Laura; Horstkotte, Burkhard; Háková, Martina; Erben, Jakub; Chvojka, Jiří; Švec, František; Solich, Petr; Garcia-Campana, Ana M.; Šatínský, DaliborA novel application of the three-dimensional printing technology for the automation of solid phase extraction procedures in a low-pressure sequential injection analysis system is presented. A 3D printed device was used as a housing for nanofiber membranes in solid phase extraction. The applicability of the device is demonstrated with the extraction of substances of various physical-chemical properties. Pharmaceuticals including non-steroidal anti-inflammatory drugs, andhistaminics, and steroidal structures, as well as emerging pollutants such as bi-sphenols and pesticide metsulfuron methyl were used as model analytes to study the extraction performance of the nanofibers. Six different nanofiber types comprising polyamide, polyethylene, polyvinylidene fluoride, polycaprolactone combined with polyvinylidene fluoride, and polyacrylonitrile, produced by electrospinning were tested in solid phase extraction. The suitability of specific nanofibers for particular analytes is demonstrated.