Porovnání analytických metod pro studium toxicity nanomateriálů
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Bakalářská práce pojednává o vlastnostech a analytických metodách pro studium nanotoxicity různých nanočástic na modelových organismech. Experimentální náplní práce bylo srovnání tří analytických metod: stanovení účinnosti fotosystému II, určení koncentrace chlorofylu a a detekce poškozených buněčných membrán u zelené řasy Chlamydomonas reinhardtii; pro studium nanotoxicity tří nanočástic: nanodiamant, magnetit a nZVI. Nanočástice byly charakterizovány pomocí metod DLS a DCS. Výsledky experimentů byly statisticky zhodnoceny použitím two-way ANOVA. Každá z analytických metod byla srovnána s nezávislým článkem a má své výhody a nevýhody, které jsou diskutovány v této práci. Nejlepší metodou bylo shledáno určení koncentrace chlorofylu a kvůli svým přesným, rychlým a stabilním výsledkům.
The bachelor thesis deals with properties and analytic method for studying nanotoxicity of different nanoparticles on model organisms. Main aim of experimental part was comparison of three different analytic methods: determination of efficiency of photosystem II, determination of concentration of chlorophyll a and detection of damage of cell membranes of green alga Chlamydomonas reinhardtii; for studying nanotoxicity of three nanoparticles: nanodiamond, magnetite and nZVI. Nanoparticles were characterized using the DLS and DCS methods. Toxicity experiments were statistically evaluated using two-way ANOVA. Each of the analytical method was compared with an independent article and has its advantages and disadvantages that are discussed in the thesis. The best method was found to be the determination of concentration of chlorophyll a due to its precise, fast and solid stable results.
The bachelor thesis deals with properties and analytic method for studying nanotoxicity of different nanoparticles on model organisms. Main aim of experimental part was comparison of three different analytic methods: determination of efficiency of photosystem II, determination of concentration of chlorophyll a and detection of damage of cell membranes of green alga Chlamydomonas reinhardtii; for studying nanotoxicity of three nanoparticles: nanodiamond, magnetite and nZVI. Nanoparticles were characterized using the DLS and DCS methods. Toxicity experiments were statistically evaluated using two-way ANOVA. Each of the analytical method was compared with an independent article and has its advantages and disadvantages that are discussed in the thesis. The best method was found to be the determination of concentration of chlorophyll a due to its precise, fast and solid stable results.
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Nanotoxicita, nanodiamant, nZVI, magnetit, Kvantový výtěžek fotosystému II, SYTOX Green, chlorofyl a, Chlamydomonas reinhardtii, DLS, DCS, Nanotoxicity, nanodiamond, nZVI, magnetite, Quantum yield of photosystem II, Sytox Green, chlorophyll a, Chlamydomonas reinhardtii, DLS, DCS