Studium částečné degradace biodegradabilních polyesterových vlákenných materiálů
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Date
2025-06-10
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Abstract
Práce se zabývá částečnou enzymatickou degradací nanovlákenných scaffoldů z PLCL a PCL a jejím vlivem na adsorpci modelového proteinu BSA. Cílem bylo stanovit vhodné koncentrace enzymů (proteinázy K a lipázy), které umožní pozorovat postupné změny materiálu bez úplné degradace. Morfologické, chemické a krystalické změny byly hodnoceny pomocí SEM, FTIR, DSC a XRD. Adsorpce BSA byla kvantifikována spektrofotometricky a potvrzena metodou SDS-PAGE.
This work deals with the partial enzymatic degradation of nanofibrous scaffolds from PLCL and PCL and its effect on the adsorption of the model protein BSA. The aim was to determine appropriate concentrations of enzymes (proteinase K and lipase) to observe gradual changes in the material without complete degradation. Morphological, chemical and crystalline changes were evaluated by SEM, FTIR, DSC and XRD. BSA adsorption was quantified spectrophotometrically and confirmed by SDS-PAGE.
This work deals with the partial enzymatic degradation of nanofibrous scaffolds from PLCL and PCL and its effect on the adsorption of the model protein BSA. The aim was to determine appropriate concentrations of enzymes (proteinase K and lipase) to observe gradual changes in the material without complete degradation. Morphological, chemical and crystalline changes were evaluated by SEM, FTIR, DSC and XRD. BSA adsorption was quantified spectrophotometrically and confirmed by SDS-PAGE.
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Tkáňové inženýrství, scaffold, biodegradabilní polyestery, nanovlákna, enzymatická degradace