Studium a výroba nanovlákenných vrstev z biodegradabilních polymerů
Loading...
Date
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Tato práce popisuje výrobu, testování a charakterizaci biodegradabilních scaffoldů pro kostní tkáně, které mají přesné poměry zastoupení mikrovláken tvořené technologií melt blown a nanovláken tvořené technologií elektrického zvlákňování. V experimentu byly vyrobeny tři druhy materiálů o stejné plošné hmotnosti a s rozdílným zastoupením nano a mikro vláken. Veškeré materiály byly vyrobeny z polykaprolaktonu. Všechny materiály se vyznačovaly optimální porézní strukturou pro buněčnou proliferaci a materiály s vyšším obsahem nanovláken prokazovaly dobré mechanické vlastnosti. Důležité strukturní vlastnosti a homogenita každého materiálu byla analyzována pomocí elektronového mikroskopu a obrazové analýzy. Vliv různého poměru nano a mikrovláken ve struktuře na adhezi a proliferaci buněk osteoblastů byl posuzován na základě in - vitro testování.
The paper describes the production process, testing and characterization of biodegradable scaffolds for bone tissue, which have the exact ratio of representation of microfibres formed by the melt blown technology and nanofibres formed by electric fiber spinning. Three kinds of materials with the same square weight and different representation of nanofibres and microfibres were produced in the experiment. All of the materials were made out of polycaprolactone. The materials were characterized by optimal porous structure for cellular poliferation and materials with higher content of nanofibres indicated good mechanical attributes. The important structural attributes and homogeneity of each material was analysed using the electron microscope and pictorial analysis. The influence of various ratio of nanofibres and microfibres in the structure on adhesion and poliferation of osteoblastic cells was evaluated on the basis of the in - vitro testing.
The paper describes the production process, testing and characterization of biodegradable scaffolds for bone tissue, which have the exact ratio of representation of microfibres formed by the melt blown technology and nanofibres formed by electric fiber spinning. Three kinds of materials with the same square weight and different representation of nanofibres and microfibres were produced in the experiment. All of the materials were made out of polycaprolactone. The materials were characterized by optimal porous structure for cellular poliferation and materials with higher content of nanofibres indicated good mechanical attributes. The important structural attributes and homogeneity of each material was analysed using the electron microscope and pictorial analysis. The influence of various ratio of nanofibres and microfibres in the structure on adhesion and poliferation of osteoblastic cells was evaluated on the basis of the in - vitro testing.
Description
Subject(s)
melt blown, elektrické zvlákňování, scaffold, nanovlákna, tkáňové inženýrství, polykaprolakton, meltblown, electrospinning, scaffold, nanofibers, tissue engineering, polycaprolactone