Optický vláknový senzor s nanovlákenným detekčním prvkem
Title Alternative:Fiber Optic Sensor with Nanofiber Detection Element
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V této práci je představen senzorický systém založený na spojení jedinečných vlastností optických vláken a nanovláken. Funkcionalizačními reakcemi byly vytvořeny imobilizované nanovlákenné vrstvy aminokyselinami a enzymem Esterázou. Optická vlákna byla použita k přenosu optického signálu, který detekoval aktivitu imobilizovaných molekul, respektive jejich schopnost vychytávat organické látky z roztoku. Ověření výsledků detekce aktivity imobilizovaných molekul bylo prováděno pomocí UV/VIS spektroskopie, SEM a FTIR spektroskopie. Práce byla posléze zaměřena na využití těchto detekčních schopností pro diagnostiku monocytární leukemie. V průběhu této práce došlo také k návrhu a konstrukci hardwaru vylepšené elektronické části senzoru.
This thesis presents a senzory system based on the combination of a unique properties of optical fibers and nanofibers. Immobilizated nanofiber layers by amino acids and Esterase enzyme were created by functionalization. Optical fibers were used to transfer an optical signal which detected the activity of the immobilizated molecules, respectively their ability to pick up organic agents from solution. Result verification of detection activity of immobilizated agents was performed by UV/VIS spektroskopy, SEM and FTIR spektroskopy. The research was further focused on using this detection ability for diagnostic of monocyte leukemia. During this work, the design and the hardware construction of the improved electronic part of the sensor was also carried out.
This thesis presents a senzory system based on the combination of a unique properties of optical fibers and nanofibers. Immobilizated nanofiber layers by amino acids and Esterase enzyme were created by functionalization. Optical fibers were used to transfer an optical signal which detected the activity of the immobilizated molecules, respectively their ability to pick up organic agents from solution. Result verification of detection activity of immobilizated agents was performed by UV/VIS spektroskopy, SEM and FTIR spektroskopy. The research was further focused on using this detection ability for diagnostic of monocyte leukemia. During this work, the design and the hardware construction of the improved electronic part of the sensor was also carried out.