Využití kopolymeru poly(L-lactide-co-e-caprolactone) pro funkcionalizaci nanovlákenných nosičů
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Abstract
Tato práce si kladla za cíl prostudovat vliv molekulové hmotnosti polymerů a poměru monomerů L-lactide a e-caprolctone v kopolymeru PLC na výsledný produkt elektrostatického zvlákňování, potažmo rozprašování a další optimalizací parametrů roztoků a procesních podmínek během rozprašování získat vhodnou strukturu kapslí pro následnou inkorporaci PVA částic. V dalším experimentu probíhal proces inkorporace PVA za pomoci metody kombinující elektrostatické zvlákňování a rozprašování. Po úspěšném začlenění PVA částic, obarvených Fluorescein-5-isothiokyanátem, byla kompozitní struktura PLC/PVA vystavena enzymatické degradaci za použití proteinázy K. V posledním experimentu byl pozorován průběh uvolňování PVA, který byl následně vyhodnocen na základě stanovení absorbance a koncentrace barviva, vyloučeného do degradačních roztoků.
This work aimed at studying the effect of polymers molecular weight and the ratio of monomers L-lactide and e-caprolctone in copolymer PLC on the final product of electrospinning, consequently electrospraying, and at obtaining a suitable structure of capsules for the subsequent incorporation of PVA particles by further optimalization of solutions parameters and process conditions during spraying. A process of PVA incorporation using the method of combining electrospinning and electrospraying took place in another experiment. After the successful incorporation of PVA particles, dyed by Fluorescein-5-isothiocyanate, the composite structure of PLC/PVA capsules was exposed to enzymatic degradation using proteinase K. In the last experiment the PVA release behavior was observed and afterwards it was evaluated by determining the absorbance and concentration of the dye, excreted into degradation solutions.
This work aimed at studying the effect of polymers molecular weight and the ratio of monomers L-lactide and e-caprolctone in copolymer PLC on the final product of electrospinning, consequently electrospraying, and at obtaining a suitable structure of capsules for the subsequent incorporation of PVA particles by further optimalization of solutions parameters and process conditions during spraying. A process of PVA incorporation using the method of combining electrospinning and electrospraying took place in another experiment. After the successful incorporation of PVA particles, dyed by Fluorescein-5-isothiocyanate, the composite structure of PLC/PVA capsules was exposed to enzymatic degradation using proteinase K. In the last experiment the PVA release behavior was observed and afterwards it was evaluated by determining the absorbance and concentration of the dye, excreted into degradation solutions.
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Poly(L-laktide-co-e-kaprolactone), Elektrospinning, Elektrospraying, Enkapsulace, Drug Delivery System, Poly(L-lactide-co-e-caprolactone), Electrospinning, Electrospraying, Encapsulation, Drug Delivery System