Cytocompatibility of polyethylene grafted with triethylenetetramine functionalized carbon nanoparticles
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Date
2017
Journal Title
Journal ISSN
Volume Title
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Abstract
Various carbon nanostructures are widely researched as scaffolds for tissue engineering. We evaluated
the surface properties and cell-substrate interactions of carbon nanoparticles functionalized with triethylenetetramine (CNPs) grafted polymer film. Two forms of polyethylene (HDPE, LDPE) were treated
in an inert argon plasma discharge and, subsequently, grafted with CNPs. The surface properties were
studied using multiple methods, including Raman spectroscopy, goniometry, atomic force microscopy, Xray photoelectron spectroscopy and electrokinetic analysis. Cell-substrate interactions were determined
in vitro by studying adhesion, proliferation and viability of vascular smooth muscle cells (VSMCs) from
the aorta of a rat. Cell-substrate interactions on pristine and modified substrates were compared to standard tissue culture polystyrene. Our results show that CNPs affect surface morphology and wettability
and therefore adhesion, proliferation and viability of cultured muscle cells.
Description
Subject(s)
Polymer grafting, Carbon nanoparticles, Cytocompatibility, Cell-substrate interactions, Wettability
Citation
ISSN
0169-4332