DNA origami nanostruktury pro medicínské aplikace
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Tato diplomová práce se zabývá využitím DNA origami v medicíně a navazuje na moji bakalářskou práci DNA origami nanostruktury pro studium radiosenzitizačního efektu nanočástic. V teoretické části jsou popsány různé postupy přípravy dvourozměrných a trojrozměrných DNA origami nanostruktur. Rovněž jsou zmíněny hlavní oblasti jejich využití v medicíně. Hlavním výsledkem experimentální části je příprava a depozice 3D DNA origami nanostruktur ve tvaru nanobloku o velikosti cca 12 nm x 12 nm x 22 nm a jejich modifikace cisplatinou a rutheniovým komplexem pro další experimenty. Dále také příprava a depozice 2D DNA origami nanostruktur ve tvaru trojúhelníku se stranou cca 120 nm a nanorámečku o velikosti cca 80 nm x 90 nm funkcionalizovaný G-quadruplexem pro další experimenty. Kromě optimalizace přípravy nanostruktur byly také provedeny vlastní experimenty studující cytotoxicitu modifikovaných DNA origami nanostruktur léčivy a poškození těchto nanostruktur laserovým zářením.
The present master's thesis is focused on the application of DNA origami in medicine. This thesis is a continuation of my bachelor's thesis DNA origami nanostruktury pro studium radiosenzitizačního efektu nanočástic (in Czech). The theoretical part of the work overviews the various approaches to the preparation of two-dimensional and three-dimensional DNA origami nanostructures. The main applications in medicine are also mentioned. The main result of the experimental part of the present thesis is the preparation and characterization of 3D DNA origami in the shape of a nanoblock with a size of cca 12 nm x 12 nm x 22 nm and their modification with cisplatin and ruthenium complex for further experiments. Other experimentally prepared nanostructures include triangular DNA origami with a side length cca 120 nm and nanoframe with a size of cca 80 nm x 90 nm functionalized with G-quadruplex for further experiments. In addition to the optimization of the preparation, further individual experiments were performed to study the cytotoxicity of drug-modified DNA origami nanostructures and the damage of triangular nanostructures under laser irradiation.
The present master's thesis is focused on the application of DNA origami in medicine. This thesis is a continuation of my bachelor's thesis DNA origami nanostruktury pro studium radiosenzitizačního efektu nanočástic (in Czech). The theoretical part of the work overviews the various approaches to the preparation of two-dimensional and three-dimensional DNA origami nanostructures. The main applications in medicine are also mentioned. The main result of the experimental part of the present thesis is the preparation and characterization of 3D DNA origami in the shape of a nanoblock with a size of cca 12 nm x 12 nm x 22 nm and their modification with cisplatin and ruthenium complex for further experiments. Other experimentally prepared nanostructures include triangular DNA origami with a side length cca 120 nm and nanoframe with a size of cca 80 nm x 90 nm functionalized with G-quadruplex for further experiments. In addition to the optimization of the preparation, further individual experiments were performed to study the cytotoxicity of drug-modified DNA origami nanostructures and the damage of triangular nanostructures under laser irradiation.
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DNA, DNA origami, DNA nanotechnologie, rakovina, doprava léčiv, cisplatina, ruthenium, samoskládání, cytotoxicita, senzor, stabilita, laser