Poly-epsilon-caprolactone Nanofibrous Polymers: A Simple Alternative to Restricted Access Media for Extraction of Small Molecules from Biological Matrixes

Simple item page
dc.contributor.authorRaabová, Hedvika
dc.contributor.authorHáková, Martina
dc.contributor.authorChocholoušová Havlíková, Lucie
dc.contributor.authorErben, Jakub
dc.contributor.authorChvojka, Jiří
dc.contributor.authorSolich, Petr
dc.contributor.authorŠvec, František
dc.contributor.authorŠatínský, Dalibor
dc.date.accessioned2020-06-17T06:49:50Z
dc.date.available2020-06-17T06:49:50Z
dc.date.issued2020-05-19
dc.description.abstractPoly-epsilon-caprolactone nanofibrous polymer has been used as an alternative to restricted access media for extraction of protein-containing biological samples and direct transfer in the chromatographic system. Three commercial cartridges differing in length and internal diameter have been manually packed with the composite material prepared from poly-epsilon-caprolactone nanofibers coated on poly-epsilon-caprolactone microfibrous scaffold and connected to the column-switching chromatographic system. Bovine milk and human serum (25 mu L) spiked with a mixture of methyl-, ethyl-, propyl-, and butylparaben in a concentration range of 1-100 mu g mL(-1) were online extracted using the cartridge-containing fibers. Then, 5 and 20% (v/v) aqueous methanol was applied as the washing mobile phase. While the ballast protein macromolecules were quantitatively eluted from the nano/microfibrous composite sorbent, the parabens were retained. After the mobile phase was switched to a stronger one, these compounds were then eluted from the extraction sorbent, directed in the analytical column, and finally separated. An extraction efficiency of 86-101% for all parabens achieved using the optimum-sized cartridge and a repeatability of the extraction procedure of 0.06-1.95% RSD were obtained.cs
dc.format.extent5 strancs
dc.identifier.doi10.1021/acs.analchem.0c00544
dc.identifier.orcid0000-0001-9856-1604 Erben, Jakub
dc.identifier.orcid0000-0001-8137-7878 Chvojka, Jiří
dc.identifier.urihttps://dspace.tul.cz/handle/15240/157195
dc.identifier.urihttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c00544
dc.language.isocscs
dc.language.isocscs
dc.publisherAMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
dc.relation.ispartofANALYTICAL CHEMISTRY
dc.subjectPERFORMANCE LIQUID-CHROMATOGRAPHYcs
dc.subjectSOLID-PHASE EXTRACTIONcs
dc.subjectSAMPLE PREPARATIONcs
dc.subjectEFFICIENCYcs
dc.subjectCOLUMNcs
dc.titlePoly-epsilon-caprolactone Nanofibrous Polymers: A Simple Alternative to Restricted Access Media for Extraction of Small Molecules from Biological Matrixescs
local.citation.epage6805
local.citation.spage6801
local.relation.issue10
local.relation.volume92
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
acs.analchem.0c00544.pdf
Size:
1.36 MB
Format:
Adobe Portable Document Format
Description:
článek
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections