Aplikace dekontaminačních technik na nanovlákenné struktury
Loading...
Date
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Diplomová práce Aplikace dekontaminačních technik na nanovlákenné struktury seznamuje čtenáře s nanomateriály a možnostmi jejich dekontaminace. Cílem je navrhnout vhodný postup dekontaminace pro vybraný typ nanovláken tak, aby byla zachována původní struktura (funkčnost) materiálu, a zároveň aby byla jeho opakovaná aplikace bezpečná pro uživatele. Hodnocení dekontaminačního postupu je řešen ze strany finální čistoty materiálu (metodou výsevu bakterií na agar a hodnocením kolonií tvořících jednotek) a ze strany míry poškození materiálu (posuzováno pomocí SEM a konfokální mikroskopie). Fotografie jsou zpracovány metodami analýzy obrazu a pomocí statistické analýzy. Výsledkem práce je uznání dekontaminačních technik Savo a UV záření aplikovaných na scaffoldy polyvinylbutyralu a polykaprolaktonu jako sterilizační. Nicméně zachování struktury se statisticky nepotvrdilo.
The Master thesis Application of decontamination techniques to nanofiber structures acquaints the reader with nanomaterials and the possibilities of their decontamination. The aim of this work is to design and verify sensitive methods for selected types of nanofibers. These methods should preserve original structure (functionality) of the material and also provide safe reusing for end-users. The decontamination methods are assessed by evaluation of final purity of the material (by the method of bacterial seeding on agar and evaluation of the colony forming units); methods are assessed using material damage evaluation (with the help of SEM and confocal microscopy). Pictures are processed by image analysis methods and results are statistically evaluated. The result of the work is to declare decontamination methods of polyvinyl butyral and polycaprolactone by Savo and UV radiation as successfully sterilizing. However, preservation of original material structure was not statistically proven.
The Master thesis Application of decontamination techniques to nanofiber structures acquaints the reader with nanomaterials and the possibilities of their decontamination. The aim of this work is to design and verify sensitive methods for selected types of nanofibers. These methods should preserve original structure (functionality) of the material and also provide safe reusing for end-users. The decontamination methods are assessed by evaluation of final purity of the material (by the method of bacterial seeding on agar and evaluation of the colony forming units); methods are assessed using material damage evaluation (with the help of SEM and confocal microscopy). Pictures are processed by image analysis methods and results are statistically evaluated. The result of the work is to declare decontamination methods of polyvinyl butyral and polycaprolactone by Savo and UV radiation as successfully sterilizing. However, preservation of original material structure was not statistically proven.
Description
Subject(s)
scaffold, nanovlákna, dekontaminační metody, sterilizace, dezinfekce, polyvinylbutyral, polykaprolakton, scaffold, nanofibers, decontamination methods, sterilization, disinfection, polyvinylbutyral, polycaprolactone