TEMPO-oxidized biodegradable bacterial cellulose (BBC) membrane coated with biologically-synthesized silver nanoparticles (AgNPs) as a potential antimicrobial agent in aquaculture (In vitro)
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Date
2020
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Abstract
The emergence of drug-resistance pathogens is one of the major challenges in aquaculture. Finding an alternative
remedy for diseases control is now crucial and indispensable. The present study aimed to develop different silver
nanocomposite BBC membranes and verified their bactericidal activity either synergistically or independently
against seven threatening aquatic pathogens (Vibrio harveyi, V. parahaemolyticus, V. alginolyticus, V. vulnificus,
Aeromonas hydrophila, A. veronii and Streptococcus iniae) using membrane disc diffusion and antibacterial log
reduction assays. The aqueous extract of Pseudomonas sp. was used for the synthesis of AgNPs and the composite
BBC materials were characterized using FTIR, XRD, EDS, and FESEM to confirm their holding capacity of integrated AgNPs. Results evidenced that the TEMPO-oxidized BBC membrane coated with bacterial-based AgNPs
exhibited an excellent crystallinity, porous properties, and strongest holding capacity. The membrane also
showed potent bactericidal activity represented by wide inhibitory zones (17–20 mm), high killing ratios
(95.93–99.86%). and high antibacterial log-reduction values (1.39–2.85). In conclusion, the synergism between
TEMPO-oxidized BBC membrane and biologically synthesized AgNPs is an eco-friendly alternative remedy to
control aquatic diseases without serious impact.
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Subject(s)
Pseudomonas sp., AgNPs, BBC membrane, Aquatic pathogens, Bactericidal activity