Browsing by Author "Solich, Petr"
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- Item3D-Printed Magnetic Stirring Cages for Semidispersive Extraction of Bisphenols from Water Using Polymer Micro- and Nanofibers(AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2020-03-03) Šrámková, Ivana; Horstkotte, Burkhard; Erben, Jakub; Chvojka, Jiří; Švec, František; Solich, Petr; Satinský, DaliborA magnetic stirring device allowing semidispersive solid phase extraction of eight bisphenols (A, AF, AP, C, BP, G, M, and Z) from river waters using polymer nano- and microfibers followed by HPLC with spectrophotometric detection has been developed and applied. About 50 mg of fibers was placed in a round, cage-like housing consisting of two identical 3D printed pieces that were locked together by a magnetic stirring bar. Magnetic stirring action of the cage devices enabled highly efficient interaction of the fibers housed inside with the aqueous samples and analyte transfer without risking fiber compaction and/or damaging. Polypropylene was found to be the best-suited filament material for the cage 3D printing, and polycaprolactone fibers appeared the most efficient sorbent out of eight tested polymers. Experimental design revealed that analytes extraction from 100 mL aqueous samples was completed within 50 min and stripping in methanol required less than 35 min. Cage housing enabled simple and robust handling of the fibrous sorbent that could be used repeatedly up to at least 5 times. Procedural repeatability was less than 5% RSD, and limits of detection and quantitation were 0.1-2.1 and 0.4-7.0 mu g L-1, respectively. Analyte recoveries at 50 mu g L-1 level ranged from 87.1% to 106.5% in the analysis of two spiked river and two lake waters.
- ItemNovel nanofibrous sorbents for the extraction and determination of resveratrol in wine(2019-07-26) Háková, Martina; Chocholoušová Havlíková, Lucie; Švec, František; Solich, Petr; Erben, Jakub; Chvojka, Jiří; Šatínský, DaliborOn-line SPE HPLC method using nanofibrous sorbents for the extraction and determination of resveratrol in wine was developed and validated. Different types of nanofibrous and microfibrous polymers were tested and compared with commercial monolithic C18 sorbent. Polyamide and polyacrylonitrile nanofibers and composite materials composed of respective polycaprolactone and poly(vinylidene difluoride) nanofibers at microfibrous scaffold were included among tested materials. Two different polycaprolactone-based materials were prepared and their effect on the extraction properties studied. Alternatively, dopamine-coated polycaprolactone fibers were also used. Poly(vinylidene difluoride) nanofibers/polycaprolactone microfibers composite was found as the most effective sorbent and utilized for the method validation. Resveratrol in red wine was determined using our validated on-line SPE HPLC method.
- ItemPoly-epsilon-caprolactone Nanofibrous Polymers: A Simple Alternative to Restricted Access Media for Extraction of Small Molecules from Biological Matrixes(AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2020-05-19) Raabová, Hedvika; Háková, Martina; Chocholoušová Havlíková, Lucie; Erben, Jakub; Chvojka, Jiří; Solich, Petr; Švec, František; Šatínský, DaliborPoly-epsilon-caprolactone nanofibrous polymer has been used as an alternative to restricted access media for extraction of protein-containing biological samples and direct transfer in the chromatographic system. Three commercial cartridges differing in length and internal diameter have been manually packed with the composite material prepared from poly-epsilon-caprolactone nanofibers coated on poly-epsilon-caprolactone microfibrous scaffold and connected to the column-switching chromatographic system. Bovine milk and human serum (25 mu L) spiked with a mixture of methyl-, ethyl-, propyl-, and butylparaben in a concentration range of 1-100 mu g mL(-1) were online extracted using the cartridge-containing fibers. Then, 5 and 20% (v/v) aqueous methanol was applied as the washing mobile phase. While the ballast protein macromolecules were quantitatively eluted from the nano/microfibrous composite sorbent, the parabens were retained. After the mobile phase was switched to a stronger one, these compounds were then eluted from the extraction sorbent, directed in the analytical column, and finally separated. An extraction efficiency of 86-101% for all parabens achieved using the optimum-sized cartridge and a repeatability of the extraction procedure of 0.06-1.95% RSD were obtained.
- ItemPolycaprolactone nanofibers functionalized with a dopamine coating for on-line solid phase extraction of bisphenols, betablockers, nonsteroidal drugs, and phenolic acids(Springer-Verlag Wien, 2019-11) Háková, Martina; Chocholoušová Havlíková, Lucie; Chvojka, Jiří; Erben, Jakub; Solich, Petr; Švec, František; Šatínský, DaliborPolycaprolactone composite nanofibers coated with a polydopamine layer are introduced as a new type of absorption material for on-line solid phase extraction (SPE) in chromatographic system. A hybrid technology combining the electrospinning and melt blowing was used for the preparation of 3D-structured microfiber/nanofibrous polycaprolactone composite. The dopamine coating was then applied to functionalize the micro/nanofibers. Polydopamine-coated polycaprolactone fibers were tested as an extraction phase in on-line SPE prior to HPLC separation and UV detection. Four groups of biologically active substances including bisphenols (Bisphenol S, Bisphenol AF, Bisphenol A, Bisphenol C, Bisphenol AP, Bisphenol Z, Bisphenol BP, and Bisphenol M), betablockers (Timolol, Metoprolol, Labetalol, and Propranolol), nonsteroidal antiphlogistic drugs (Salicylic acid, Ketoprofen, Naproxen, Indomethacin, Diclofenac, Ibuprophen, and Meclofenamic acid), and phenolic acids (Chlorogenic acid, Caffeic acid, Sinapic acid, m-Coumaric acid, Benzoic acid, and Cinnamic acid) were used as the model analytes. Neat and coated fibers were compared and applied as sorbents for the on-line extraction set-up. Both materials produced good extraction potential for the determination of bisphenols and nonsteroidal drugs in model biological and environmental samples including river water, human urine, and blood serum. However, the polydopamine layer significantly increased the extraction efficiency of polar drugs. Typical repeatability of on-line extraction procedure on polydopamine coated fibers was in the range 0.12–4.11% for bisphenols, 0.55–1.41% for antiphlogistic drugs, 0.59–2.52% for phenolic acids, and 1.01–1.65% for betablockers.
- ItemScreening of extraction properties of nanofibers in a sequential injection analysis system using a 3D printed device(ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, 2019-05-15) Šrámková, Ivana H.; Carbonell-Rozas, Laura; Horstkotte, Burkhard; Háková, Martina; Erben, Jakub; Chvojka, Jiří; Švec, František; Solich, Petr; Garcia-Campana, Ana M.; Šatínský, DaliborA novel application of the three-dimensional printing technology for the automation of solid phase extraction procedures in a low-pressure sequential injection analysis system is presented. A 3D printed device was used as a housing for nanofiber membranes in solid phase extraction. The applicability of the device is demonstrated with the extraction of substances of various physical-chemical properties. Pharmaceuticals including non-steroidal anti-inflammatory drugs, andhistaminics, and steroidal structures, as well as emerging pollutants such as bi-sphenols and pesticide metsulfuron methyl were used as model analytes to study the extraction performance of the nanofibers. Six different nanofiber types comprising polyamide, polyethylene, polyvinylidene fluoride, polycaprolactone combined with polyvinylidene fluoride, and polyacrylonitrile, produced by electrospinning were tested in solid phase extraction. The suitability of specific nanofibers for particular analytes is demonstrated.