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Browsing by Author "Amler Evžen"

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    Cell Penetration to Nanofibrous Scaffolds
    (Cell adhesion & migration, 2014-01-01) Rampichova Michala; Chvojka Jiří; Amler Evžen; Kofroňová Olga; Prosecká Eva; Buzgo Matej
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    Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration
    (Informa UK Limited, 2018-01-01) Rampichova Michala; Kuželová Košťáková Eva; Filová Eva; Chvojka Jiří; Šafka Jiří; Pelcl Martin; Dankova J; Prosecka Eva; Buzgo Matej; Plencner Martin; Lukáš David; Amler Evžen
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    Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration
    (Taylor&Francis Online, 2018-01-01) Rampichova Michala; Kuželová Košťáková Eva; Filová Eva; Chvojka Jiří; Šafka Jiří; Pelcl Martin; Daňková, J.; Prosecka Eva; Buzgo Matej; Plencner Martin; Lukáš David; Amler Evžen
    Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.
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    Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits
    (MDPI AG, 2020-01-01) Filova Eva; Tonar Zbyněk; Lukášová Věra; Buzgo Matej; Litvinec Andrej; Rampichova Michala; Beznoska Jiří; Plencner Martin; Staffa Andrea; Dankova J; Soural Miroslav; Chvojka Jiří; Malečková Anna; Králíčková Milena; Amler Evžen
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    Method for producing a polymer nanofibers by spinning a solvent solution or melt of polymer in electric field, and the linear form of the polymer nanofibers prepared by this method
    (2018-01-01) Kočiš Lubomír; Pokorný Pavel; Lukáš David; Mikeš Petr; Chvojka Jiří; Kuželová Košťáková Eva; Beran Jaroslav; Bílek Martin; Valtera Jan; Amler Evžen; Buzgo Matej; Míčková Andrea
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    Method for production of polymeric nanofibers and linear formation from polymeric nanofibers prepared by this method
    (2018-01-01) Kočiš Lubomír; Pokorný Pavel; Lukáš David; Mikeš Petr; Chvojka Jiří; Kuželová Košťáková Eva; Beran Jaroslav; Bílek Martin; Valtera Jan; Amler Evžen; Buzgo Matej; Míčková Andrea
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    Method for production of polymeric nanofibers by spinning of solution or melt of polymer in electric field
    (2018-01-01) Kočiš Lubomír; Pokorný Pavel; Lukáš David; Mikeš Petr; Chvojka Jiří; Kuželová Košťáková Eva; Beran Jaroslav; Bílek Martin; Valtera Jan; Amler Evžen; Buzgo Matej; Míčková Andrea
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    Method for production of polymeric nanofibers by spinning of solution or melt of polymer in electric field, and a linear formation from polymeric nanofibers prepared by this method
    (2017-01-01) Pokorný Pavel; Lukáš David; Mikeš Petr; Chvojka Jiří; Kuželová Košťáková Eva; Beran Jaroslav; Bílek Martin; Valtera Jan; Amler Evžen; Buzgo Matej; Míčková Andrea; Lubomír Kociš
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    Method for production of polymeric nanofibers by spinning of solution or melt of polymer in electric field, and a linear formation from polymeric nanofibers prepared by this method
    (2020-01-01) Lubomír Kociš; Pokorný Pavel; Lukáš David; Mikeš Petr; Chvojka Jiří; Kuželová Košťáková Eva; Beran Jaroslav; Bílek Martin; Valtera Jan; Amler Evžen; Buzgo Matěj; Míčková Andrea
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    Method for production of polymeric nanofibers by spinning of solution or melt of polymer in electric field, and a linear formation from polymeric nanofibers prepared by this method
    (2019-01-01) Kočiš Lubomír; Pokorný Pavel; Lukáš David; Mikeš Petr; Chvojka Jiří; Kuželová Košťáková Eva; Beran Jaroslav; Bílek Martin; Valtera Jan; Amler Evžen; Míčková Andrea; Buzgo Matej
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    Method of producing a nanofibrous layer from an aqueous solution of polyvinyl alcohol for tissue engineering by electrostatic spinning by a needleless or needle electrostatic spinning method
    (2019-01-01) Louda Petr; Rozek Zbigniew; Fijalkowski Mateusz; Lukáš David; Chvojka Jiří; Bajáková Jana; Vysloužilová Lucie; Amler Evžen; Buzgo Matej; Bezděková Dagmar; Lukášová Věra
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    Method of producing functional nanofibrous layer and apparatus for making the same
    (2011-01-01) Pokorný Pavel; Lukáš David; Mikeš Petr; Vysloužilová Lucie; Chvojka Jiří; Hégrová Božena; Amler Evžen; Buzgo Matej; Louda Petr
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    Needleless coaxial electrospinning: A novel approach to mass production of coaxial nanofibers
    (ELSEVIER SCIENCE BV, 2017-01-01) Vysloužilová Lucie; Buzgo Matej; Pokorný Pavel; Chvojka Jiří; Míčková Andrea; Rampichova Michala; Kula Jiří; Pejchar Karel; Bílek Martin; Lukáš David; Amler Evžen
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    Polycaprolactone foam functionalized with chitosan microparticles– a suitable scaffold for cartilage regeneration
    (Institute of Physiology, Czech Academy of Sciences, 2016-01-01) Filová Eva; Jakubcová Barbora; Danilová Iveta; Košťáková Eva; Jarošíková Taťána; Chernyavskiy Oleksandr; Hejda Jan; Handl Milan; Beznoska Jiří; Nečas Alois; Rosina Jozef; Amler Evžen
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    Procedure for the production of polymeric nanofibers by spinning a polymer solution or melt in an electric field, and linear formation of polymeric nanofibers prepared by this procedure
    (2020-01-01) Lubomír Kociš; Pokorný Pavel; Lukáš David; Mikeš Petr; Chvojka Jiří; Kuželová Košťáková Eva; Beran Jaroslav; Bílek Martin; Valtera Jan; Amler Evžen; Buzgo Matěj; Míčková Andrea
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    The combination of nanofibrous and microfibrous materials for enhancement of cell infiltration and in vivo bone tissue formation
    (IOP Publishing, 2018-01-01) Rampichova Michala; Chvojka Jiří; Jenčová Věra; Kubíková Tereza; Tonar Zbyněk; Erben Jakub; Buzgo Matej; Dankova J; Litvinec Andrej; Vocetková K; Plencner Martin; Prosecka Eva; Sovková V; Lukášová Věra; Králíčková Milena; Lukáš David; Amler Evžen

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