Strategies adopted by Aphanizomenon flos-aquae in response to phosphorus deficiency and their role on growth

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dc.contributor.authorChen, Xiaoyan
dc.contributor.authorDolinová, Iva
dc.contributor.authorŠevců, Alena
dc.contributor.authorJurczak, Tomasz
dc.contributor.authorFrankiewicz, Piotr
dc.contributor.authorWojtal-Frankiewicz, Adrianna
dc.contributor.authorWan, Lingling
dc.contributor.authorDeng, Qinghui
dc.contributor.authorSong, Chunlei
dc.contributor.authorZhou, Yiyong
dc.contributor.authorCao, Xiuyun
dc.date.accessioned2020-04-03T12:00:29Z
dc.date.available2020-04-03T12:00:29Z
dc.date.issued2020-03-16
dc.description.abstractBackground The N-2-fixing cyanobacterium, Aphanizomenon flos-aquae is a globally distributed bloom causing species that degrades water quality of fresh and marine water bodies. Overcoming phosphorus (P) deficiency is one of the ecological advantages for bloom-forming cyanobacteria. It remains unclear to what extent can A. flos-aquae alleviate P deficiency by regulating P using strategies. Results Based on in situ observations of extracellular alkaline phosphatase (APase) in A. flos-aquae via enzyme-labeled fluorescence in freshwater bodies in China, Poland and Czechia, we further investigated responses of isolated A. flos-aquae to different P supplies (dissolved inorganic P (Pi) as +DIP, dissolved organic alpha-glycerophosphate and beta-glycerophosphate as +DOP alpha and +DOP beta, P-free condition as P-depleted). The significantly negative relationships between percentage of APase producing cells and soluble reactive P concentration in both fields and cultures suggested that the excretion of APase in cyanobacterium was regulated by ambient Pi supply. Suffering from P deficiency in the P-depleted treatments, A. flos-aquae showed the highest APase activity but a vigorous growth at the early culture stage, which might also benefit from the formation of polyphosphate body (PPB) and the decrease of cell P quota. In the +DOP treatments, the coordination of dissolved DOP mineralization and continuous prompt utilization of PPB might contribute to a maintenance but not reproduction of A. flos-aquae when relying on DOP, since the specific growth rate kept around 0 cells L-1 day(-1) at the second half culture period and the highest cell density reached only 13.38% of that in +DIP treatments while photochemical efficiency was comparable during the whole experiment. Luxury uptake of phosphate as PPB in the +DIP treatments was consistent to the canonical view of polyphosphate as P storage. Conclusion Aphanizomenon flos-aquae could achieve an instantaneous growth in response to P deficiency with the coordination of P utilization strategies, while it maintained a long-term sustainable growth but not reproduction under sole DOP supply. Persistent and active reproduction could only be achieved in high Pi supply, which implying that an effective consequence can be expected for combating the bloom of A. flos-aquae when controlling P supply.cs
dc.format.extent13 strancs
dc.identifier.doi10.1186/s12302-020-00328-3
dc.identifier.orcid0000-0001-8772-704X Ševců, Alena
dc.identifier.urihttps://dspace.tul.cz/handle/15240/154695
dc.identifier.urihttps://enveurope.springeropen.com/articles/10.1186/s12302-020-00328-3
dc.language.isocscs
dc.publisherSPRINGEROPEN, CAMPUS, 4 CRINAN ST, LONDON, N1 9XW, ENGLAND
dc.relation.ispartofENVIRONMENTAL SCIENCES EUROPE
dc.subjectAphanizomenon flos-aquaecs
dc.subjectP deficiencycs
dc.subjectExtracellular alkaline phosphatasecs
dc.subjectPolyphosphate bodycs
dc.subjectStrategies for P utilizationcs
dc.titleStrategies adopted by Aphanizomenon flos-aquae in response to phosphorus deficiency and their role on growthcs
local.article.number45
local.relation.issue1
local.relation.volume32
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