Repetitive detection of aromatic hydrocarbon contaminants with bioluminescent bioreporters attached on tapered optical fiber elements

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dc.contributor.authorZajíc Jakubcs
dc.contributor.authorRipp Stevencs
dc.contributor.authorTrögl Josefcs
dc.contributor.authorKuncová Gabrielacs
dc.contributor.authorPospíšilová Mariecs
dc.date.accessioned2021-01-14T18:03:40Z
dc.date.available22-19-2020en
dc.date.available2021-01-14T18:03:40Z
dc.date.issued2021cs
dc.date.updated22-04-2020en
dc.description.abstractIn this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used.en
dc.format.extent0cs
dc.identifier.doi10.3390/s20113237
dc.identifier.issn1424-8220cs
dc.identifier.urihttps://dspace.tul.cz/handle/15240/158484
dc.identifier.urihttps://www.mdpi.com/1424-8220/20/11/3237
dc.language.isoengcs
dc.publisherMDPI AGcs
dc.relation.ispartofSensors (Switzerland)en
dc.relation.ispartofseries0cs
dc.relation.urihttps://www.mdpi.com/1424-8220/20/11/3237/pdfcs
dc.subjectwhole-cell biosensorcs
dc.subjectbioluminescent bioreportercs
dc.subjectoptical fiber biosensorcs
dc.subjecttoluenecs
dc.subjectPseudomonas putida TVA8cs
dc.subjectEscherichia coli 652T7cs
dc.titleRepetitive detection of aromatic hydrocarbon contaminants with bioluminescent bioreporters attached on tapered optical fiber elementscs
local.citation.epage1-13cs
local.citation.spage1-13cs
local.identifier.publikace7811
local.identifier.wok000552737900230en
local.relation.issue11cs
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