Beta3 adrenoceptors substitute the role of M 2 muscarinic receptor in coping with cold stress in the heart: Evidence from M 2KO mice

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dc.contributor.authorBeneš, Jan
dc.contributor.authorNováková, Martina
dc.contributor.authorRotková, Jana
dc.contributor.authorFarár, Vladimír
dc.contributor.authorKvětňanský, Richard
dc.contributor.authorRiljak, V.
dc.contributor.authorMysliveček, Jaromír B.
dc.date.accessioned2015-10-26
dc.date.available2015-10-26
dc.date.issued2012
dc.description.abstractWe investigated the role of beta3-adrenoceptors (AR) in cold stress (1 or 7 days in cold) in animals lacking main cardioinhibitive receptors - M2 muscarinic receptors (M 2KO). There was no change in receptor number in the right ventricles. In the left ventricles, there was decrease in binding to all cardiostimulative receptors (beta1-, and beta2-AR) and increase in cardiodepressive receptors (beta3-AR) in unstressed KO in comparison to WT. The cold stress in WT animals resulted in decrease in binding to beta1- and beta2-AR (to 37%/35% after 1 day in cold and to 27%/28% after 7 days in cold) while beta3-AR were increased (to 216% of control) when 7 days cold was applied. MR were reduced to 46% and 58%, respectively. Gene expression of M2 MR in WT was not changed due to stress, while M3 was changed. The reaction of beta1- and beta2-AR (binding) to cold was similar in KO and WT animals, and beta3-AR in stressed KO animals did not change. Adenylyl cyclase activity was affected by beta3-agonist CL316243 in cold stressed WT animals but CL316243 had almost no effects on adenylyl cyclase activity in stressed KO. Nitric oxide activity (NOS) was not affected by BRL37344 (beta3-agonist) both in WT and KO animals. Similarly, the stress had no effects on NOS activity in WT animals and in KO animals. We conclude that the function of M2 MR is substituted by beta3-AR and that these effects are mediated via adenylyl cyclase rather than NOS. © Springer Science+Business Media, LLC 2011.en
dc.formattext
dc.identifier.doi10.1007/s10571-011-9781-3
dc.identifier.issn0272-4340
dc.identifier.scopus2-s2.0-84864718022
dc.identifier.urihttps://dspace.tul.cz/handle/15240/13299
dc.language.isoen
dc.publisherTechnická Univerzita v Libercics
dc.publisherTechnical university of Liberec, Czech Republicen
dc.relation.ispartofCellular and Molecular Neurobiologyen
dc.sourcej-scopus
dc.subjectadenylyl cyclaseen
dc.subjectbeta-adrenoceptorsen
dc.subjectcold stressen
dc.subjecthearten
dc.subjectmuscarinic receptorsen
dc.subjectnitric oxide synthaseen
dc.titleBeta3 adrenoceptors substitute the role of M 2 muscarinic receptor in coping with cold stress in the heart: Evidence from M 2KO miceen
dc.typeArticle
local.citation.epage869
local.citation.spage859
local.facultyInstitute of Health Studies
local.fulltextyes
local.identifier.codenCMNED
local.identifier.pubmed22222438
local.relation.issue5
local.relation.volume32
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