Wastewater Treatment by Novel Polyamide/Polyethylenimine Nanofibers with Immobilized Laccase

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dc.contributor.authorMaryšková, Milena
dc.contributor.authorSchaabová, Markéta
dc.contributor.authorTománková, Hana
dc.contributor.authorNovotný, Vít
dc.contributor.authorRysová, Miroslava
dc.date.accessioned2020-02-24T07:09:24Z
dc.date.available2020-02-24T07:09:24Z
dc.date.issued2020-02-24
dc.description.abstractEndocrine-disrupting chemicals are highly resistant organic compounds, commonly occurring in the aquatic environment, that can interfere with the endocrine system of animals and humans, causing serious chronic diseases. In recent decades, enzymes from oxidoreductases have been studied for their potential to degrade these compounds effectively. In order to use such enzymes repeatedly, it is necessary to ensure their insolubility in water, a method termed enzyme immobilization. We developed novel polyamide/polyethylenimine (PA/PEI) nanofibers as a promising support material for the immobilization of various biomolecules. Our nanofibers are highly suitable due to a unique combination of mechanical endurance provided by polyamide 6 and their affinity toward biomolecules, ensured by numerous PEI amino groups. Enzyme laccase was successfully immobilized onto PA/PEI nanofibers using a simple and fast method, providing exceptional activity and stability of the attached enzyme. We then tested the degradation ability of the PA/PEI-laccase samples on a highly concentrated mixture of endocrine-disrupting chemicals in real wastewater with adjusted pH. The results indicate that the samples were a suitable material for wastewater treatment by degrading a highly concentrated mixture of bisphenol A, 17α-ethinylestradiol, triclosan, and diclofenac, in real wastewater effluent.cs
dc.format.extent12 strancs
dc.identifier.WebofScienceResearcherIDG-5999-2017 Tománková, Hana
dc.identifier.WebofScienceResearcherID0000-0001-8083-5419 Novotný, Vít
dc.identifier.doi10.3390/w12020588
dc.identifier.orcid0000-0001-7500-0150 Maryšková, Milena
dc.identifier.orcid0000-0003-3289-4021 Tománková, Hana
dc.identifier.orcid0000-0003-3395-6941 Rysová, Miroslava
dc.identifier.urihttps://dspace.tul.cz/handle/15240/154428
dc.identifier.urihttps://www.mdpi.com/2073-4441/12/2/588
dc.language.isocscs
dc.publisherMDPI
dc.relation.ispartofWater 2020
dc.subjectwastewater treatmentcs
dc.subjectendocrine-disrupting chemicalscs
dc.subjectnanofiberscs
dc.subjectlaccasecs
dc.subjectimmobilizationcs
dc.titleWastewater Treatment by Novel Polyamide/Polyethylenimine Nanofibers with Immobilized Laccasecs
local.relation.issue2
local.relation.volume12
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