A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane

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dc.contributor.authorBačáková, Markéta
dc.contributor.authorPajorova, Julia
dc.contributor.authorBrož, Antonín
dc.contributor.authorHadraba, Daniel
dc.contributor.authorLopot, František
dc.contributor.authorZavaďáková, Anna
dc.contributor.authorVištejnová, Lucie
dc.contributor.authorBeno, Milan
dc.contributor.authorKostič, Ivan
dc.contributor.authorJenčová, Věra
dc.contributor.authorBačáková, Lucie
dc.date.accessioned2019-07-26T06:14:19Z
dc.date.available2019-07-26T06:14:19Z
dc.date.issued2019-07-08
dc.description.abstractBackground: Repairs to deep skin wounds continue to be a difficult issue in clinical practice. A promising approach is to fabricate full-thickness skin substitutes with functions closely similar to those of the natural tissue. For many years, a three-dimensional (3D) collagen hydrogel has been considered to provide a physiological 3D environment for co-cultivation of skin fibroblasts and keratinocytes. This collagen hydrogel is frequently used for fabricating tissue-engineered skin analogues with fibroblasts embedded inside the hydrogel and keratinocytes cultivated on its surface. Despite its unique biological properties, the collagen hydrogel has insufficient stiffness, with a tendency to collapse under the traction forces generated by the embedded cells. Methods: The aim of our study was to develop a two-layer skin construct consisting of a collagen hydrogel reinforced by a nanofibrous poly-L-lactide (PLLA) membrane pre-seeded with fibroblasts. The attractiveness of the membrane for dermal fibroblasts was enhanced by coating it with a thin nanofibrous fibrin mesh. Results: The fibrin mesh promoted the adhesion, proliferation and migration of the fibroblasts upwards into the collagen hydrogel. Moreover, the fibroblasts spontaneously migrating into the collagen hydrogel showed a lower tendency to contract and shrink the hydrogel by their traction forces. The surface of the collagen was seeded with human dermal keratinocytes. The keratinocytes were able to form a basal layer of highly mitotically-active cells, and a suprabasal layer. Conclusion: The two-layer skin construct based on collagen hydrogel with spontaneously immigrated fibroblasts and reinforced by a fibrin-coated nanofibrous membrane seems to be promising for the construction of full-thickness skin substitute.cs
dc.format.extent18 strancs
dc.identifier.doi10.2147/IJN.S200782
dc.identifier.urihttps://dspace.tul.cz/handle/15240/152974
dc.identifier.urihttps://www.dovepress.com/a-two-layer-skin-construct-consisting-of-a-collagen-hydrogel-reinforce-peer-reviewed-article-IJN
dc.language.isocscs
dc.publisherDovepress
dc.relation.ispartofInternational Journal of Nanomedicine
dc.subjectfull-thickness skin substitutescs
dc.subjectcollagen hydrogelcs
dc.subjectfibroblast and keratinocyte co-cultivationcs
dc.subjectfibrincs
dc.subjectnanostructurecs
dc.titleA two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membranecs
local.citation.epage5050
local.citation.spage5033
local.identifier.publikace7101
local.relation.volume14
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