The genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: A pilot study

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dc.contributor.authorNovotná, Božena
dc.contributor.authorSikorová, Jitka
dc.contributor.authorMilcová, Alena
dc.contributor.authorPechout, Martin
dc.contributor.authorDittrich, Luboš
dc.contributor.authorVojtíšek-Lom, Michal
dc.contributor.authorRossner, Pavel
dc.contributor.authorBrzičová, Táňa
dc.contributor.authorTopinka, Jan
dc.date.accessioned2019-10-22T07:14:55Z
dc.date.available2019-10-22T07:14:55Z
dc.date.issued2019-10
dc.description.abstractAn analysis of the toxic effects of emissions should reflect real traffic conditions. The exhaust emissions of particulate matter from diesel engines strongly depend on their operating conditions, with low-speed, low-load "urban creep" conditions, common for truck traffic in heavily congested urban areas, being one of the worst. We aimed to detect the genotoxicity of organic extracts from particulate matter in the exhaust of the diesel engine Zetor 1505 running on diesel and biodiesel (B100) fuels at characteristic modes of extended "urban creep", typical for transit truck traffic in Prague, comparing the first 5 min of idling with extended (20-80 min) idling, full load after idle, "stabilized" full load, and 30% load. The diluted exhaust was sampled with high volume samplers on glass fiber fluorocarbon coated filters. The filters were extracted with dichloromethane and DNA damage was analyzed in A549 cells using comet assay, with the inclusion of formamidopyrimidine DNA glycosylase (FPG) and endonuclease III (ENDOIII) to recognize oxidized DNA bases. The cells were exposed to extractable organic matter (EOM) for 4 and 24 h at non-cytotoxic dose corresponding to 0.001 m(3) of undiluted exhaust gas per ml cell media. At the 4 h exposure interval, all samples from B100 and diesel emissions induced DNA damage. EOM from the extended idle engine mode exerted the strongest genotoxic effect for both fuels. Twenty hours later, the cells exposed to diesel EOM exhibited a further increase of DNA strand breaks compared to the preceding interval. In contrast, DNA damage seemed to be fully repaired in cells treated with EOM derived from biodiesel B100. The preliminary results suggest that (i) diesel emissions are more genotoxic than the emissions from B100, (ii) biodiesel induced DNA lesions are repaired within 24 h.cs
dc.format.extent6 strancs
dc.identifier.doi10.1016/j.mrgentox.2019.03.007
dc.identifier.urihttps://dspace.tul.cz/handle/15240/154042
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1383571818303462
dc.language.isocscs
dc.publisherELSEVIER, RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS
dc.relation.ispartofMUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
dc.subjectA549 cellscs
dc.subjectBiodieselcs
dc.subjectComet assaycs
dc.subjectExhaust emissionscs
dc.subjectGenotoxicitycs
dc.titleThe genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: A pilot studycs
local.article.numberUNSP 403034
local.identifier.publikace6349
local.relation.volume845
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