A comparison study of nanofiber, microfiber, and new composite nano/microfiber polymers used as sorbents for on-line solid phase extraction in chromatography system

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dc.contributor.authorHakova Martinacs
dc.contributor.authorChocholousova Havlikova Luciecs
dc.contributor.authorChvojka Jiřícs
dc.contributor.authorErben Jakubcs
dc.contributor.authorSolich Petrcs
dc.contributor.authorŠvec Františekcs
dc.date.accessioned2018-09-25T12:19:48Z
dc.date.available2018-09-25T12:19:48Z
dc.date.issued2018cs
dc.description.abstractThree different approaches has been used to obtain nano/micro fibers and their diversity and extraction properties were examined. The effect of their structure on stability in an ultra-high-performance liquid chromatography (UHPLC) system during on-line SPE procedure was monitored. Five types of various nano/micro fiber polymers were used as sorbents: polyamide 6 nanofibers, polyvinylidene difluoride nanofibers, polyethylene microfibers, and two new polycaprolactone microfiber/nanofiber and polycaprolactone microfibers/polyvinylidene difluoride nanofibers composite polymers. The fiber polymers were filled in a cartridge directly connected to the UHPLC system and tested. For each polymer, the optimal conditions of the on-line extraction were found and potential applicability on real samples was tested. The determination of ochratoxin A (OTA) in beer matrix was chosen as a case study. Relevant factors such as the mechanical and chemical stability of the nano/microfibers, filling the cartridges, fiber reusability and the possibility and the repeatability of all processes were involved in the proposed study. A new nano/micro composite sorbent consisting of polycaprolactone microfibers/polyvinylidene difluoride nanofibers was chosen as the most suitable sorbent for the on-line extraction of OTA from a beer matrix. The tested validation parameters had the value of intra-day precision lower than 1.48%, linearity in the range from 0.5 to 100 μg L−1 with r2 ≥ 0.9999 for standard and matrix calibration curve, and recovery in the range 99.1–103.9% at five concentration levels. Long-term precision evaluated for 31 analyses over the period of three months did not exceed 2.9% RSD. It confirmed the column reusability and perfect stability of nano/micro composite sorbent in the presence of organic solvents and after repeated injection of a complex beer matrix.en
dc.format.extent9cs
dc.identifier.doihttps://doi.org/10.1016/j.aca.2018.04.023
dc.identifier.issn0003-2670cs
dc.identifier.urihttps://dspace.tul.cz/handle/15240/32013
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S000326701830504X?via=ihub
dc.language.isoengcs
dc.publisherElsevier Ltd.cs
dc.relation.ispartofseries1cs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S000326701830504X?via=ihubcs
dc.rights.SHERPA/RoMEOAnalytica Chimica Acta (ISSN: 0003-2670, ESSN: 1873-4324), author cannot archive publisher's version/PDF
dc.subjectPolyamide 6cs
dc.subjectPolyvinylidene difluoridecs
dc.subjectPolycaprolactonecs
dc.subjectPolyethylene nanofiberscs
dc.subjectOn-line extractioncs
dc.subjectOchratoxin Acs
dc.titleA comparison study of nanofiber, microfiber, and new composite nano/microfiber polymers used as sorbents for on-line solid phase extraction in chromatography systemcs
local.citation.epage44-52cs
local.citation.spage44-52cs
local.identifier.publikace5484
local.identifier.wok000432351500005en
local.relation.issueSEP 6 2018cs
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