A comparison study of nanofiber, microfiber, and new composite nano/microfiber polymers used as sorbents for on-line solid phase extraction in chromatography system
dc.contributor.author | Hakova Martina | cs |
dc.contributor.author | Chocholousova Havlikova Lucie | cs |
dc.contributor.author | Chvojka Jiří | cs |
dc.contributor.author | Erben Jakub | cs |
dc.contributor.author | Solich Petr | cs |
dc.contributor.author | Švec František | cs |
dc.date.accessioned | 2018-09-25T12:19:48Z | |
dc.date.available | 2018-09-25T12:19:48Z | |
dc.date.issued | 2018 | cs |
dc.description.abstract | Three different approaches has been used to obtain nano/micro fibers and their diversity and extraction properties were examined. The effect of their structure on stability in an ultra-high-performance liquid chromatography (UHPLC) system during on-line SPE procedure was monitored. Five types of various nano/micro fiber polymers were used as sorbents: polyamide 6 nanofibers, polyvinylidene difluoride nanofibers, polyethylene microfibers, and two new polycaprolactone microfiber/nanofiber and polycaprolactone microfibers/polyvinylidene difluoride nanofibers composite polymers. The fiber polymers were filled in a cartridge directly connected to the UHPLC system and tested. For each polymer, the optimal conditions of the on-line extraction were found and potential applicability on real samples was tested. The determination of ochratoxin A (OTA) in beer matrix was chosen as a case study. Relevant factors such as the mechanical and chemical stability of the nano/microfibers, filling the cartridges, fiber reusability and the possibility and the repeatability of all processes were involved in the proposed study. A new nano/micro composite sorbent consisting of polycaprolactone microfibers/polyvinylidene difluoride nanofibers was chosen as the most suitable sorbent for the on-line extraction of OTA from a beer matrix. The tested validation parameters had the value of intra-day precision lower than 1.48%, linearity in the range from 0.5 to 100 μg L−1 with r2 ≥ 0.9999 for standard and matrix calibration curve, and recovery in the range 99.1–103.9% at five concentration levels. Long-term precision evaluated for 31 analyses over the period of three months did not exceed 2.9% RSD. It confirmed the column reusability and perfect stability of nano/micro composite sorbent in the presence of organic solvents and after repeated injection of a complex beer matrix. | en |
dc.format.extent | 9 | cs |
dc.identifier.doi | https://doi.org/10.1016/j.aca.2018.04.023 | |
dc.identifier.issn | 0003-2670 | cs |
dc.identifier.uri | https://dspace.tul.cz/handle/15240/32013 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S000326701830504X?via=ihub | |
dc.language.iso | eng | cs |
dc.publisher | Elsevier Ltd. | cs |
dc.relation.ispartofseries | 1 | cs |
dc.relation.uri | https://www.sciencedirect.com/science/article/pii/S000326701830504X?via=ihub | cs |
dc.rights.SHERPA/RoMEO | Analytica Chimica Acta (ISSN: 0003-2670, ESSN: 1873-4324), author cannot archive publisher's version/PDF | |
dc.subject | Polyamide 6 | cs |
dc.subject | Polyvinylidene difluoride | cs |
dc.subject | Polycaprolactone | cs |
dc.subject | Polyethylene nanofibers | cs |
dc.subject | On-line extraction | cs |
dc.subject | Ochratoxin A | cs |
dc.title | A comparison study of nanofiber, microfiber, and new composite nano/microfiber polymers used as sorbents for on-line solid phase extraction in chromatography system | cs |
local.citation.epage | 44-52 | cs |
local.citation.spage | 44-52 | cs |
local.identifier.publikace | 5484 | |
local.identifier.wok | 000432351500005 | en |
local.relation.issue | SEP 6 2018 | cs |