Aligned hydrogel tubes guide regeneration following spinal cord injury

Simple item page
dc.contributor.authorCarlson, Mitchell A.
dc.contributor.authorDumont, Courtney M.
dc.contributor.authorMunsell, Mary K.
dc.contributor.authorCiciriello, Andrew J.
dc.contributor.authorStrnadová, Kateřina
dc.contributor.authorPark, Jonghyuck
dc.contributor.authorCummings, Brian J.
dc.contributor.authorAnderson, Aileen J.
dc.contributor.authorShea, Lonnie D.
dc.date.accessioned2019-10-04T07:01:52Z
dc.date.available2019-10-04T07:01:52Z
dc.date.issued2019-03-01
dc.description.abstractDirecting the organization of cells into a tissue with defined architectures is one use of biomaterials for regenerative medicine. To this end, hydrogels are widely investigated as they have mechanical properties similar to native soft tissues and can be formed in situ to conform to a defect. Herein, we describe the development of porous hydrogel tubes fabricated through a two-step polymerization process with an intermediate microsphere phase that provides macroscale porosity (66.5%) for cell infiltration. These tubes were investigated in a spinal cord injury model, with the tubes assembled to conform to the injury and to provide an orientation that guides axons through the injury. Implanted tubes had good apposition and were integrated with the host tissue due to cell infiltration, with a transient increase in immune cell infiltration at 1 week that resolved by 2 weeks post injury compared to a gelfoam control. The glial scar was significantly reduced relative to control, which enabled robust axon growth along the inner and outer surface of the tubes. Axon density within the hydrogel tubes (1744 axons/mm(2)) was significantly increased more than 3-fold compared to the control (456 axons/mm(2)), with approximately 30% of axons within the tube myelinated. Furthermore, implantation of hydrogel tubes enhanced functional recovery relative to control. This modular assembly of porous tubes to fill a defect and directionally orient tissue growth could be extended beyond spinal cord injury to other tissues, such as vascular or musculoskeletal tissue.cs
dc.format.extent11 strancs
dc.identifier.doi10.1016/j.actbio.2018.12.052
dc.identifier.urihttps://dspace.tul.cz/handle/15240/153834
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1742706119300017
dc.language.isocscs
dc.publisherELSEVIER SCI LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
dc.relation.ispartofACTA BIOMATERIALIA
dc.subjectModular biomaterialcs
dc.subjectTissue repaircs
dc.subjectSpinal cord injurycs
dc.subjectAxon elongationcs
dc.titleAligned hydrogel tubes guide regeneration following spinal cord injurycs
local.citation.epage322
local.citation.spage312
local.relation.volume86
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Aligned hydrogel tubes guide regeneration following spinal cord injury.pdf
Size:
2.57 MB
Format:
Adobe Portable Document Format
Description:
článek
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections