Polyamide-Laccase Nanofiber Membrane for Degradation of Endocrine-Disrupting Bisphenol A, 17 alpha-ethinylestradiol, and Triclosan
| dc.contributor.author | Maryšková, Milena | |
| dc.contributor.author | Rysová, Miroslava | |
| dc.contributor.author | Novotný, Vít | |
| dc.contributor.author | Ševců, Alena | |
| dc.date.accessioned | 2020-01-28T08:03:14Z | |
| dc.date.available | 2020-01-28T08:03:14Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Contamination of potable water by endocrine disrupting chemicals (EDCs) is a growing problem worldwide. One of the possible treatments is the utilization of laccase enzyme catalyzing oxidation of phenolic structures of EDC when anchored in a polymeric nanofiber membrane. Previous studies failed to develop a membrane with a sufficiently active enzyme, or the immobilization process was too complicated and time-consuming. Here, we established an elegant method for immobilizing Trametes versicolor laccase onto polyamide 6 nanofibers (PA6-laccase) via adsorption and glutaraldehyde crosslinking, promoting high enzyme activity and easier applicability in water treatment technology. This simple and inexpensive immobilization ensures both repeated use, with over 88% of initial activity retained after five ABTS catalytic cycles, and enhanced storage stability. PA6-laccase was highly effective in degrading a 50-mu M EDC mixture, with only 7% of bisphenol A, 2% of 17 alpha-ethinylestradiol, and 30% of triclosan remaining after a 24-h catalytic process. The PA6-laccase membrane can lead to the improvement of novel technologies for controlling of EDC contamination in potable water. | cs |
| dc.format.extent | 14 stran | cs |
| dc.identifier.doi | 10.3390/polym11101560 | |
| dc.identifier.orcid | 0000-0001-7500-0150 Maryšková, Milena | |
| dc.identifier.orcid | 0000-0001-8772-704X Ševců, Alena | |
| dc.identifier.uri | https://dspace.tul.cz/handle/15240/154375 | |
| dc.identifier.uri | https://www.mdpi.com/2073-4360/11/10/1560 | |
| dc.language.iso | cs | cs |
| dc.publisher | MDPI | |
| dc.relation.ispartof | Polymers | |
| dc.subject | laccase | cs |
| dc.subject | polyamide 6 | cs |
| dc.subject | nanofibers | cs |
| dc.subject | wastewater treatment | cs |
| dc.subject | endocrine disrupting chemicals | cs |
| dc.title | Polyamide-Laccase Nanofiber Membrane for Degradation of Endocrine-Disrupting Bisphenol A, 17 alpha-ethinylestradiol, and Triclosan | cs |
| local.access | open access | |
| local.article.number | 1560 | |
| local.relation.issue | 10 | |
| local.relation.volume | 11 |