Composite hyaluronate-type I collagen-fibrin scaffold in the therapy of osteochondral defects in miniature pigs

Simple item page
dc.contributor.authorFilová, Eva
dc.contributor.authorRampichová, Michala
dc.contributor.authorHandl, Milan
dc.contributor.authorLytvynets, Andriy
dc.contributor.authorHalouzka, Roman
dc.contributor.authorUsvald, Dušan
dc.contributor.authorHlučílová, Jana
dc.contributor.authorProcházka, Radek
dc.contributor.authorDezortová, Monika
dc.contributor.authorRolencová, Eva
dc.contributor.authorKošťáková Kuželová, Eva
dc.contributor.authorTrč, Tomáš
dc.contributor.authorŠťastný, Eduard
dc.contributor.authorKoláčná, Lucie
dc.contributor.authorHájek, Milan
dc.contributor.authorMotlík, Jan
dc.contributor.authorAmler, Evžen
dc.date.accessioned2016-05-24
dc.date.available2016-05-24
dc.date.issued2007
dc.description.abstractThe potential of novel scaffold containing sodium hyaluronate, type I collagen, and fibrin was investigated in the regeneration of osteochondral defects in miniature pigs. Both autologous chondrocyte-seeded scaffolds and non-seeded scaffolds were implanted into two defects located in the non-weight-bearing zone of the femoral trochlea (defect A was located more distally and medially, defect B was located more proximally and laterally). Control defects were left untreated. Twelve weeks after the operation, the knees were evaluated in vivo using MRI Six months after the implantation, the defects were analyzed using MRI, histological, and immunohistochernical analysis. In the A defects of chondrocyte-seeded scaffold group, hyaline cartilage and fibrocartilage was formed, containing type II collagen, acidic and neutral glycosaminoglycans while the non-seeded scaffold group was predominantly filled with fibrocartilage. Defects in the control group were predominantly filled with fibrous tissue. Histomorphometric analysis of photomicrographs revealed a significantly higher amount of hyaline cartilage in the cell-seeded scaffold group in A defects than in other groups. Both scaffold groups in A defects showed significantly less fibrous tissue than cell-seeded defects B and the control group. Both histological and MRI analysis proved that the novel composite scaffold has a potential to regenerate osteochondral defects within six months.en
dc.formattext
dc.identifier.issn0862-8408
dc.identifier.scopus2-s2.0-38549158588
dc.identifier.urihttps://dspace.tul.cz/handle/15240/16418
dc.language.isoen
dc.publisherAcad Sciences Czech Republic, Inst Physiology
dc.publisherTechnická Univerzita v Libercics
dc.publisherTechnical university of Liberec, Czech Republicen
dc.relation.ispartofPhysiological Researchen
dc.sourcej-scopus
dc.sourcej-wok
dc.subjectosteochondral defecten
dc.subjectfibrinen
dc.subjectautologous chondrocytesen
dc.subjectcartilage regenerationen
dc.titleComposite hyaluronate-type I collagen-fibrin scaffold in the therapy of osteochondral defects in miniature pigsen
dc.typeArticle
local.accessopen
local.citation.epage16
local.citation.spage5
local.departmentDepartment of nonwovens and nanofiber materials
local.facultyFaculty of Textile Engineering
local.fulltextyes
local.identifier.pubmed17552900
local.identifier.stagRIV/68378041:_____/07:00095024
local.identifier.wok252436800002
local.relation.volume56
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
composite.pdf
Size:
12.05 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections