Experimental fortification of intestinal anastomoses with nanofibrous materials in a large animal model

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dc.contributor.authorRosendorf, Jáchym
dc.contributor.authorHoráková, Jana
dc.contributor.authorKlíčová, Markéta
dc.contributor.authorPálek, Richard
dc.contributor.authorČervenková, Lenka
dc.contributor.authorKural, Tomáš
dc.contributor.authorHošek, Petr
dc.contributor.authorKříž, Tomáš
dc.contributor.authorTégl, Václav
dc.contributor.authorMoulisová, Vladimíra
dc.contributor.authorTonar, Zbyněk
dc.contributor.authorTreska, Vladislav
dc.contributor.authorLukáš, David
dc.contributor.authorLiska, Václav
dc.date.accessioned2020-02-11T08:22:35Z
dc.date.available2020-02-11T08:22:35Z
dc.date.issued2020
dc.description.abstractAnastomotic leakage is a severe complication in gastrointestinal surgery. It is often a reason for reoperation together with intestinal passage blockage due to formation of peritoneal adhesions. Different materials as local prevention of these complications have been studied, none of which are nowadays routinely used in clinical practice. Nanofabrics created proved to promote healing with their structure similar to extracellular matrix. We decided to study their impact on anastomotic healing and formation of peritoneal adhesions. We performed an experiment on 24 piglets. We constructed 3 hand sutured end-to-end anastomoses on the small intestine of each pig. We covered the anastomoses with a sheet of polycaprolactone nanomaterial in the first experimental group, with a sheet of copolymer of polylactic acid with polycaprolactone in the second one and no fortifying material was used in the Control group. The animals were sacrificed after 3 weeks of observation. Clinical, biochemical and macroscopic signs of anastomotic leakage or intestinal obstruction were monitored, the quality of the scar tissue was assessed histologically, and a newly developed scoring system was employed to evaluate the presence of adhesions. The material is easy to manipulate with. There was no mortality or major morbidity in our groups. No statistical difference was found inbetween the groups in the matter of level of peritoneal adhesions or the quality of the anastomoses. We created a new adhesion scoring system. The material appears to be safe however needs to be studied further to prove itsʹ positive effects.cs
dc.format.extent12 strancs
dc.identifier.WebofScienceResearcherIDA-8450-2018 Horáková, Jana
dc.identifier.WebofScienceResearcherIDG-9288-2014 Lukáš, David
dc.identifier.doi10.1038/s41598-020-58113-4
dc.identifier.orcid0000-0003-2926-0570 Horáková, Jana
dc.identifier.orcid0000-0003-4037-6435 Lukáš, David
dc.identifier.urihttps://dspace.tul.cz/handle/15240/154399
dc.identifier.urihttps://www.nature.com/articles/s41598-020-58113-4
dc.language.isocscs
dc.publisherNature Research
dc.relation.ispartofScientific Reports
dc.subjectAnastomotic Leakcs
dc.subjectStoma closurecs
dc.subjectRectal Neoplasmscs
dc.titleExperimental fortification of intestinal anastomoses with nanofibrous materials in a large animal modelcs
local.accessopen access
local.article.number1134
local.identifier.publikace7087
local.relation.issue1
local.relation.volume10
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