Hydrochemical Conditions for Aerobic/Anaerobic Biodegradation of Chlorinated Ethenes—A Multi-Site Assessment

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dc.contributor.authorNěmeček, Jan
dc.contributor.authorMarková, Kristýna
dc.contributor.authorŠpánek, Roman
dc.contributor.authorAntoš, Vojtěch
dc.contributor.authorKozubek, Petr
dc.contributor.authorLhotský, Ondřej
dc.contributor.authorČerník, Miroslav
dc.date.accessioned2020-01-23T07:15:24Z
dc.date.available2020-01-23T07:15:24Z
dc.date.issued2020-01-23
dc.description.abstractA stall of cis-1,2-DCE and vinyl chloride (VC) is frequently observed during bioremediation of groundwater chloroethenes via reductive dechlorination. These chloroethenes may be oxidised by aerobic methanotrophs or ethenotrophs co-metabolically and/or metabolically. We assessed the potential for such oxidation at 12 sites (49 groundwater samples) using hydrochemical and molecular biological tools. Both ethenotroph (etnC and etnE) and methanotroph (mmoX and pmoA) functional genes were identified in 90% of samples, while reductive dehalogenase functional genes (vcrA and bvcA) were identified in 82%. All functional genes were simultaneously detected in 78% of samples, in actively biostimulated sites in 88% of samples. Correlation analysis revealed that cis-1,2-DCE concentration was positively correlated with vcrA, etnC and etnE, while VC concentration was correlated with etnC, etnE, vcrA and bvcA. However, feature selection based on random forest classification indicated a significant relationship for the vcrA in relation to cis-1,2-DCE, and vcrA, bvcA and etnE for VC and no prove of relationship between cis-1,2-DCE or VC and the methanotroph functional genes. Analysis of hydrochemical parameters indicated that aerobic oxidation of chloroethenes by ethenotrophs may take place under a range of redox conditions of aquifers and coincide with high ethene and VC concentrations.cs
dc.format.extent18 strancs
dc.identifier.WebofScienceResearcherIDB-4390-2013 Černík, Miroslav
dc.identifier.doi10.3390/w12020322
dc.identifier.orcid0000-0001-5967-4614 Němeček, Jan
dc.identifier.orcid0000-0002-0520-3494 Marková, Kristýna
dc.identifier.orcid0000-0002-7554-020X Černík, Miroslav
dc.identifier.urihttps://dspace.tul.cz/handle/15240/154368
dc.identifier.urihttps://www.mdpi.com/2073-4441/12/2/322
dc.language.isocscs
dc.publisherMDPI
dc.relation.ispartofWater 2020
dc.subjectchlorinated solventscs
dc.subjectbiological reductive dechlorinationcs
dc.subjectaerobic oxidationcs
dc.subjectqPCRcs
dc.subjectethenotrophscs
dc.subjectmethanotrophscs
dc.titleHydrochemical Conditions for Aerobic/Anaerobic Biodegradation of Chlorinated Ethenes—A Multi-Site Assessmentcs
local.accessopen access
local.relation.issue2
local.relation.volume12
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