Thermally enhanced in situ bioremediation of groundwater contaminated with chlorinated solvents; A field test

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dc.contributor.authorNěmeček Jancs
dc.contributor.authorSteinová Janacs
dc.contributor.authorŠpánek Romancs
dc.contributor.authorPluhař Tomášcs
dc.contributor.authorPokorný Petrcs
dc.contributor.authorNajmanová Petracs
dc.contributor.authorKnytl Vladislavcs
dc.contributor.authorČerník Miroslavcs
dc.date.accessioned2018-09-25T12:12:38Z
dc.date.available2018-09-25T12:12:38Z
dc.date.issued2018cs
dc.description.abstractIn situ bioremediation (ISB) using reductive dechlorination is a widely accepted but relatively slow approach compared to other technologies for the treatment of groundwater contaminated by chlorinated ethenes (CVOCs). Due to the known positive kinetic effect on microbial metabolism, thermal enhancement may be a viable means of accelerating ISB. We tested thermally enhanced ISB in aquifers situated in sandy saprolite and underlying fractured granite. The system comprised pumping, heating and subsequent injection of contaminated groundwater aiming at an aquifer temperature of 20–30 °C. A fermentable substrate (whey) was injected in separate batches. The test was monitored using hydrochemical and molecular tools (qPCR and NGS). The addition of the substrate and increase in temperature resulted in a rapid increase in the abundance of reductive dechlorinators (e.g., Dehalococcoides mccartyi, Dehalobacter sp. and functional genes vcrA and bvcA) and a strong increase in CVOC degradation. On day 34, the CVOC concentrations decreased by 87% to 96% in groundwater from the wells most affected by the heating and substrate. On day 103, the CVOC concentrations were below the LOQ resulting in degradation half-lives of 5 to 6 days. Neither an increase in biomarkers nor a distinct decrease in the CVOC concentrations was observed in a deep well affected by the heating but not by the substrate. NGS analysis detected Chloroflexi dechlorinating genera (Dehalogenimonas and GIF9 and MSBL5 clades) and other genera capable of anaerobic metabolic degradation of CVOCs. Of these, bacteria of the genera Acetobacterium, Desulfomonile, Geobacter, Sulfurospirillum, Methanosarcina and Methanobacterium were stimulated by the substrate and heating. In contrast, groundwater from the deep well (affected by heating only) hosted representatives of aerobic metabolic and aerobic cometabolic CVOC degraders. The test results document that heating of the treated aquifer significantly accelerated the treatment process but only in the case of an abundant substrate.en
dc.format.extent13cs
dc.identifier.doi10.1016/j.scitotenv.2017.12.047
dc.identifier.issn0048-9697cs
dc.identifier.urihttps://dspace.tul.cz/handle/15240/30983
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0048969717334666?via%3Dihub
dc.language.isoengcs
dc.publisherElsevier BVcs
dc.publisher.cityHolandskocs
dc.relation.ispartofseries1cs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0048969717334666cs
dc.subjectChlorinated solventscs
dc.subjectThermally enhanced bioremediationcs
dc.subjectBiological reductive dechlorinationcs
dc.subjectIndigenous microorganismscs
dc.subjectqPCRcs
dc.subjectNGScs
dc.titleThermally enhanced in situ bioremediation of groundwater contaminated with chlorinated solvents; A field testen
local.citation.epage743-755cs
local.citation.spage743-755cs
local.identifier.publikace4438
local.identifier.wok426349000077en
local.relation.issue2018cs
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