Impact of Various Sterilization and Disinfection Techniques on Electrospun Poly-epsilon-caprolactone

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dc.contributor.authorHoráková, Jana
dc.contributor.authorKlíčová, Markéta
dc.contributor.authorErben, Jakub
dc.contributor.authorKlápšťová, Andrea
dc.contributor.authorNovotný, Vít
dc.contributor.authorBěhálek, Luboš
dc.contributor.authorChvojka, Jiří
dc.date.accessioned2020-05-13T06:15:52Z
dc.date.available2020-05-13T06:15:52Z
dc.date.issued2020-04-21
dc.description.abstractElectrospun materials made from biodegradable polycaprolactone are used widely in various tissue engineering and regenerative medicine applications because of their morphological similarity to the extracellular matrix. However, the main prerequisite for the use of such materials in clinical practice consists of the selection of the appropriate sterilization technique. This study is devoted to the study of the impact of traditional sterilization and disinfection methods on a nanofibrous polycaprolactone layer constructed by means of the needleless electrospinning technique. It was determined that hydrogen peroxide plasma treatment led to the loss of fibrous morphology and the creation of a foil. However, certain sterilization (ethylene oxide, gamma irradiation, and peracetic acid) and disinfection techniques (ethanol and UV irradiation) were found not to lead to a change in morphology; thus, the study investigates their impact on thermal properties, molecular weight, and interactions with a fibroblast cell line. It was determined that the surface properties that guide cell adhesion and proliferation were affected more than the bulk properties. The highest proliferation rate of fibroblasts seeded on nanofibrous scaffolds was observed with respect to gamma-irradiated polycaprolactone, while the lowest proliferation rate was observed following ethylene oxide sterilization.cs
dc.format.extent8 strancs
dc.identifier.WebofScienceResearcherIDA-8450-2018 Horáková, Jana
dc.identifier.doi10.1021/acsomega.0c00503
dc.identifier.orcid0000-0003-2926-0570 Horáková, Jana
dc.identifier.orcid0000-0001-9856-1604 Erben, Jakub
dc.identifier.orcid0000-0002-6924-800X Klápšťová, Andrea
dc.identifier.orcid0000-0002-8749-3586 Běhálek, Luboš
dc.identifier.orcid0000-0001-8137-7878 Chvojka, Jiří
dc.identifier.urihttps://dspace.tul.cz/handle/15240/154811
dc.identifier.urihttps://pubs.acs.org/doi/abs/10.1021/acsomega.0c00503
dc.language.isocscs
dc.publisherAMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
dc.relation.ispartofACS OMEGA
dc.subjectGAMMA-IRRADIATIONcs
dc.subjectSILK FIBROINcs
dc.subjectSCAFFOLDScs
dc.subjectPROLIFERATIONcs
dc.subjectBIOMATERIALcs
dc.subjectNANOFIBERcs
dc.subjectPCLcs
dc.subjectNANOcs
dc.titleImpact of Various Sterilization and Disinfection Techniques on Electrospun Poly-epsilon-caprolactonecs
local.citation.epage8892
local.citation.spage8885
local.relation.issue15
local.relation.volume5
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