The restructuring of dopamine receptor subtype gene transcripts in c-fos KO mice

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dc.contributor.authorBeneš, Jan
dc.contributor.authorMravec, Boris
dc.contributor.authorKrižanova, Oľga
dc.contributor.authorKvětňanský, Richard
dc.contributor.authorMysliveček, Jaromír B.
dc.date.accessioned2015-10-26
dc.date.available2015-10-26
dc.date.issued2012
dc.description.abstractAlthough c-Fos protein is one of the principal molecules in intracellular signaling, c-fos gene disruption is associated with alterations in neuronal functions that do not correspond to its importance in function. The aim of the study was to evaluate the changes of dopaminergic system together with acetylcholinesterase (AChE) in c-fos disruption (KO). KO male mice showed an increase in D 1-like receptor (279% of WT) and D 2-like receptor (345% of WT) binding sites in the cortex. On the gene expression level (assessed by real-time PCR), lower quantities of D 1R-mRNA (0.64) and D 5R-mRNA (0.6) were found in females when compared to males in the frontal cortex, higher D 2R-mRNA in the parietal (1.43) and temporal (2.64) cortex and lower AChE-mRNA (0.67). On the contrary, female striatum contained higher level of D 2R-mRNA (1.62) and AChE-mRNA (1.57) but lower level of D 3R-mRNA (0.73). Hypothalamic D 1R-mRNA, D 2R-mRNA and D 4R-mRNA were higher in females (1.38, 1.63, and 1.68, respectively). Disruption of c-fos increased selectively D 5R-mRNA (1.31) in male parietal cortex, D 2R-mRNA (1.72) in male temporal cortex, and cerebellar D 2R-mRNA in both males (1.43) and females (1.42), respectively. In females, we found rather decrease in DR-mRNA. Multiple correlations in mRNA quantities (in WT mice) were found, which changed considerably upon c-fos KO. Main interactions in WT were inter-regional, CNS of KO underwent an extensive restructuring comprising intraregional interactions in the frontal cortex, hypothalamus, and cerebellum. These changes in DR (between others) could be considered as one of the adaptive mechanisms in c-fos KO mice. © 2012 Elsevier Inc.en
dc.formattext
dc.identifier.doi10.1016/j.brainresbull.2012.02.006
dc.identifier.issn0361-9230
dc.identifier.scopus2-s2.0-84859158438
dc.identifier.urihttps://dspace.tul.cz/handle/15240/13301
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.publisherTechnická Univerzita v Libercics
dc.publisherTechnical university of Liberec, Czech Republicen
dc.relation.ispartofBrain Research Bulletinen
dc.sourcej-scopus
dc.sourcej-wok
dc.subjectc-fosen
dc.subjectdopamine receptorsen
dc.subjectcerebral cortexen
dc.subjectcerebellumen
dc.subjecthypothalamusen
dc.subjectstriatumen
dc.subjecthippocampusen
dc.titleThe restructuring of dopamine receptor subtype gene transcripts in c-fos KO miceen
dc.typeArticle
local.citation.epage520
local.citation.spage511
local.facultyInstitute of Health Studies
local.fulltextyes
local.identifier.codenBRBUD
local.identifier.pubmed22374467
local.identifier.wok303487300003
local.relation.issue6
local.relation.volume87
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