Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

Simple item page
dc.contributor.authorFilová, Eva
dc.contributor.authorTonar, Zbyněk
dc.contributor.authorLukášová, Věra
dc.contributor.authorBuzgo, Matěj
dc.contributor.authorLitvinec, Andrej
dc.contributor.authorRampichová, Michala
dc.contributor.authorBeznoska, Jiří
dc.contributor.authorPlecner, Martin
dc.contributor.authorStaffa, Andrea
dc.contributor.authorDaňková, Jana
dc.contributor.authorSoural, Miroslav
dc.contributor.authorChvojka, Jiří
dc.contributor.authorMalečková, Anna
dc.contributor.authorKrálíčková, Milena
dc.contributor.authorAmler, Evžen
dc.date.accessioned2020-09-24T12:06:03Z
dc.date.available2020-09-24T12:06:03Z
dc.date.issued2020
dc.description.abstractHydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested; fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µm) and further modified by polyethylene glycol–biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation.cs
dc.format.extent27 strancs
dc.identifier.doi10.3390/nano10081504
dc.identifier.orcid0000-0001-8137-7878 Chvojka, Jiří
dc.identifier.urihttps://dspace.tul.cz/handle/15240/157801
dc.identifier.urihttps://www.mdpi.com/2079-4991/10/8/1504
dc.language.isocscs
dc.publisherMDPI
dc.relation.ispartofNanomaterials 2020
dc.subjectpoly-ε-caprolactonecs
dc.subjectcartilagecs
dc.subjectCD44 antibodycs
dc.subjectcollagencs
dc.subjectfibrincs
dc.subjectmicroparticlescs
dc.titleHydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbitscs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Hydrogel Containing.pdf
Size:
4.5 MB
Format:
Adobe Portable Document Format
Description:
článek
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections