Stratification of chlorinated ethenes natural attenuation in an alluvial aquifer assessed by hydrochemical and biomolecular tools

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dc.contributor.authorNěmeček Jancs
dc.contributor.authorDolinová Ivacs
dc.contributor.authorMacháčková Jiřinacs
dc.contributor.authorŠpánek Romancs
dc.contributor.authorŠevců Alenacs
dc.contributor.authorLederer Tomášcs
dc.contributor.authorČerník Miroslavcs
dc.date.accessioned2018-09-25T12:12:23Z
dc.date.available2018-09-25T12:12:23Z
dc.date.issued2017cs
dc.description.abstractBiomolecular and hydrochemical tools were used to evaluate natural attenuation of chlorinated ethenes in a Quaternary alluvial aquifer located close to a historical source of large-scale tetrachloroethylene (PCE) contamination. Distinct stratification of redox zones was observed, despite the aquifer's small thickness (2.8 m). The uppermost zone of the target aquifer was characterised by oxygen- and nitrate-reducing conditions, with mixed iron- to sulphate-reducing conditions dominant in the lower zone, along with indications of methanogenesis. Natural attenuation of PCE was strongly influenced by redox heterogeneity, while higher levels of PCE degradation coincided with iron- to sulphate reducing conditions. Next generation sequencing of the middle and/or lower zones identified anaerobic bacteria (Firmicutes, Chloroflexi, Actinobacteria and Bacteroidetes) associated with reductive dechlorination. The relative abundance of dechlorinators (Dehalococcoides mccartyi, Dehalobacter sp.) identified by real-time PCR in soil from the lower levels supports the hypothesis that there is a significant potential for reductive dechlorination of PCE. Local conditions were insufficiently reducing for rapid complete dechlorination of PCE to harmless ethene. For reliable assessment of natural attenuation, or when designing monitoring or remedial systems, vertical stratification of key biological and hydrochemical markers should be analysed as standard, even in shallow aquifers.en
dc.format.extent11cs
dc.identifier.WebofScienceResearcherIDB-4390-2013 Černík Miroslav
dc.identifier.doi10.1016/j.chemosphere.2017.06.100
dc.identifier.issn0045-6535cs
dc.identifier.orcid0000-0001-5967-4614 Němeček Jan
dc.identifier.orcid0000-0002-4854-3720 Špánek Roman
dc.identifier.orcid0000-0001-8772-704X Ševců Alena
dc.identifier.orcid0000-0002-7554-020X Černík Miroslav
dc.identifier.urihttps://dspace.tul.cz/handle/15240/30945
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0045653517310044?via%3Dihub
dc.language.isoengcs
dc.publisherElseviercs
dc.relation.ispartofseries0cs
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/28672697cs
dc.subjectChlorinated solventscs
dc.subjectNatural attenuationcs
dc.subjectIndigenous microorganismscs
dc.subjectHydrochemical markerscs
dc.subjectBiological markerscs
dc.titleStratification of chlorinated ethenes natural attenuation in an alluvial aquifer assessed by hydrochemical and biomolecular toolsen
local.citation.epage1157-1167cs
local.citation.spage1157-1167cs
local.identifier.publikace4399
local.identifier.wok407525500129en
local.relation.issueOctober 2017cs
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