Browsing by Author "Votrubová Martina"
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- ItemRemediation of Chlorinated Ethenes Using Reactive Iron Barrier and Its Impact on Indigenous Bacteria(International ASET Inc, 2014) Czinnerová Marie; Ševců Alena; Dolinová Iva; Votrubová MartinaChlorinated ethenes are among the most often detected organic contaminants ingroundwater. Under anaerobic conditions, tetrachloroethene (PCE) and trichloroethene (TCE) can be degraded by the sequence of reductive dechlorination steps through the intermediate products cis-dichloroethene (cis-DCE) and vinyl chloride (VC) to ethene. This process is driven either by specific microorganisms or by reductive agents, such as nano or microscale zero-valent iron particles (nZVI or mZVI). Apermeable reactive barrierwas prepared by applying a mixture of nZVI and mZVI to the series of wells downstream fromthe source of the contamination. Polymerase chain reaction (PCR) and real-time PCR analysis of microbial composition of groundwater upstream and downstream of the barrier revealed strong influence of iron-driven dechlorination on the recovery of microbial colonization of groundwater including bacteria capable of degrading chlorinated ethenes.