Browsing by Author "Steinová Jana"
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- ItemAnaerobic microbial corrosion of carbon steel under conditions relevant for deep geological repository of nuclear waste(2021-01-01) Shrestha Rojina; Černoušek Tomáš; Stoulil Jan; Kovářová Hana; Sihelská Kristína; Špánek Roman; Ševců Alena; Steinová Jana
- ItemAssessment of biodegradation potential at a site contaminated by a mixture of BTEX, chlorinated pollutants and pharmaceuticals using passive sampling methods; Case study(ELSEVIER SCIENCE BV, 2017-01-01) Lhotský Ondřej; Krákorová Eva; Linhartová Lucie; Křesinová Zdena; Steinová Jana; Dvořák Lukáš; Rodsand Torgeir; Filipová Alena; Kroupová Kristýna; Wimmerová Lenka; Kukačka Jan; Cajthaml TomášThe present study describes a pilot remediation test of a co-mingled plume containing BTEX, chlorinated pollutants and pharmaceuticals. Remediation was attempted using a combination of various approaches, including a pump and treat system applying an advanced oxidation process and targeted direct push injections of calcium peroxide. The remediation process was monitored intensively and extensively throughout the pilot test using various conventional and passive sampling methods, including next-generation amplicon sequencing. The results showed that the injection of oxygen-saturated treated water with residual hydrogen peroxide and elevated temperature enhanced the in situ removal of monoaromatics and chlorinated pollutants. In particular, in combination with the injection of calcium peroxide, the conditions facilitated the in situ bacterial biodegradation of the pollutants. The mean groundwater concentration of benzene decreased from 1349 μg·L− 1 prior to the test to 3 μg·L− 1 within 3 months after the calcium peroxide injections; additionally, monochlorobenzene decreased from 1545 μg·L− 1 to 36 μg·L− 1, and toluene decreased from 143 μg·L− 1 to 2 μg·L− 1. Furthermore, significant degradation of the contaminants bound to the soil matrix in less permeable zones was observed. Based on a developed 3D model, 90% of toluene and 88% of chlorobenzene bound to the soil were removed during the pilot test, and benzene was removed almost completely. On the other hand, the psychopharmaceuticals were effectively removed by the employed advanced oxidation process only from the treated water, and their concentration in groundwater remained stagnant due to inflow from the surroundings and their absence of in situ degradation. The employment of passive sampling techniques, including passive diffusion bags (PDB) for volatile organic pollutants and their respective transformation products, polar organic compound integrative samplers (POCIS) for the pharmaceuticals and in situ soil microcosms for microbial community analysis, was proven to be suitable for monitoring remediation in saturated zones.
- ItemCombination of nanoscale-zero-valent iron and organic sub-strate stimulation for efficient remediation of co-mingled plume contaminated with Cr(VI) and chlorinated solvents(2016-01-01) Cajthaml Tomáš; Němeček Jan; Pokorný Petr; Lhotský Ondřej; Knytl Vladislav; Najmanová Petra; Steinová Jana; Černík Miroslav; Filipová Alena; Filip Jan
- ItemCombined nano-biotechnology for in-situ remediation of mixed contamination of groundwater by hexavalent chromium and chlorinated solvents(ELSEVIER SCIENCE BV, 2016-01-01) Němeček Jan; Pokorný Petr; Lhotský Ondřej; Knytl Vladislav; Najmanová Petra; Steinová Jana; Černík Miroslav; Filipová Alena; Filip Jan; Cajthaml Tomáš
- ItemEvaluation of two different methods for endotoxin detection in nanoparticle suspensions(2016-01-01) Steinová Jana; Bobčíková Kateřina; Hristov Delyan R.; Ševců Alena
- ItemLarge beech (Fagus sylvatica) trees as 'lifeboats' for lichen diversity in central European forests(SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS, 2016-01-01) Hofmeister Jenyk; Hošek Jan; Malíček Jiří; Palice Zdeněk; Syrovátkova Lada; Steinová Jana; Černajová Ivana
- ItemMicrobial corrosion in deep geological repository, effect on canister for spent nuclear fuel(2015-01-01) Shrestha Rojina; Ševců Alena; Černík Miroslav; Dobrev David; Špaček Pavel; Stoulil Jan; Steinová Jana
- ItemMICROBIAL CORROSION UNDER DEEP GEOLOGICAL HIGH-LEVEL RADIOACTIVE WASTE REPOSITORY CONDITIONS FOR CONCEPT STEEL-BASED CANISTER - COMPACTED BENTONITE - project report year 2016(2016-01-01) Černík Miroslav; Steinová Jana; Ševců Alena; Mikeš Jiří; Špaček Pavel; Dobrev David; Černoušek Tomáš; Stoulil Jan
- ItemMicrobial Corrosion Under Deep Geological High-level Radioactive Waste Repository Conditions for Concept Steel-based Canister - Compacted Bentonite - Project Report Year 2016(2017-01-01) Černík Miroslav; Steinová Jana; Ševců Alena; Burkartová Kateřina; Černá Kateřina; Mikeš Jiří; Pavel Špaček; Dobrev David; Černoušek Tomáš; Stoulil Jan
- ItemMicrobial corrosion under the conditions of a deep geological repository for the concept of steel UOS - compacted bentonite(2019-01-01) Černík Miroslav; Steinová Jana; Špaček Pavel; Mikeš Jiří; Dobrev David; Černoušek Tomáš; Stoulil Jan
- ItemMicrobial screening at the Bukov URF and the Rožná mine Final report of the project(2019-01-01) Steinová Jana; Burkartová Kateřina; Mikeš Jiří; Zuna Milan; Černík Miroslav
- ItemMicrobially influenced corrosion of carbon steel in the presence of anaerobic sulphate-reducing bacteria(Taylor and Francis, 2020-01-01) Shrestha Rojina; Špánek Roman; Ševců Alena; Steinová Jana
- ItemMicrobially influenced corrosion of container material(Elsevier, 2020-01-01) Shrestha Rojina; Ševců Alena; Steinová Jana
- ItemMicrobially influenced corrosion of container material(Elsevier, 2020-01-01) Černoušek Tomáš; Ševců Alena; Shrestha Rojina; Steinová Jana; Kokinda Jakub; Vizelková Kateřina
- ItemMolecular techniques for understanding microbial abundance and activity in wasteforms(Elsevier, 2020-01-01) Steinová Jana; Hlaváčková Veronika; Černá Kateřina
- ItemPharmaceuticals, benzene, toluene and chlorobenzene removal from contaminated groundwater by combined UV/H2O2 photo-oxidation and aeration(2017-01-01) Lhotský Ondřej; Krákorová Eva; Mašín Pavel; Žebrák Radim; Linhartová Lucie; Křesinová Zdena; Kašlík Josef; Steinová Jana; Rodsand Torgeir; Filipová Alena; Petrů Klára; Kroupová Kristýna; Cajthaml TomášThis study was performed to test the feasibility of several decontamination methods for remediatingheavily contaminated groundwater in a real contaminated locality in the Czech Republic, where apharmaceuticals plant has been in operation for more than 80 years. The site is polluted mainly byrecalcitrant psychopharmaceuticals and monoaromatic hydrocarbons, such as benzene, toluene andchlorobenzene. For this purpose, an advanced oxidation technique employing UV radiation withhydrogen peroxide dosing was employed, in combination with simple aeration pretreatment. The resultsshowed that UV/H2O2was an efficient and necessary step for degradation of the pharmaceuticals;however, the monoaromatics were already removed during the aeration step. Characterization of theremoval mechanisms participating in the aeration revealed that volatilization, co-precipitation andbiodegradation contributed to the process. Thesefindings were supported by bacterial metabolite ana-lyses, phospholipid fatty acid analysis, qPCR of representatives of the degradative genes and detailedcharacterization of the formed precipitate using M€ossbauer spectroscopy and scanning electron micro-scopy. Further tests were carried out in a continuous arrangement directly connected to the wells alreadypresent in the locality. The results documented the feasibility of combination of the photo-reactoremploying UV/H2O2together with aeration pretreatment for 4 months, where the overall decontami-nation efficiency ranged from 72% to 99% of the pharmaceuticals. We recorded even better results for themonoaromatics decontamination except for one month, when we encountered some technical problemswith the aeration pump. This demonstrated the necessity of using the aeration step.
- ItemSurvival of microorganisms in bentonite subjected to different levels of irradiation and pressure(2019-01-01) Černá Kateřina; Černoušek Tomáš; Polívka Petr; Ševců Alena; Steinová Jana
- ItemThe effect of Caesium ions on a natural anaerobic microbial community(Czech Environment Management Center, 2018-01-01) Shrestha Rojina; Steinová Jana; Ševců Alena; Černoušek Tomáš; Kovářová Hana; Jakub Kokinda; Romana Hadravová
- ItemThermally enhanced in situ bioremediation of groundwater contaminated with chlorinated solvents; A field test(Elsevier BV, 2018-01-01) Němeček Jan; Steinová Jana; Špánek Roman; Pluhař Tomáš; Pokorný Petr; Najmanová Petra; Knytl Vladislav; Černík MiroslavIn situ bioremediation (ISB) using reductive dechlorination is a widely accepted but relatively slow approach compared to other technologies for the treatment of groundwater contaminated by chlorinated ethenes (CVOCs). Due to the known positive kinetic effect on microbial metabolism, thermal enhancement may be a viable means of accelerating ISB. We tested thermally enhanced ISB in aquifers situated in sandy saprolite and underlying fractured granite. The system comprised pumping, heating and subsequent injection of contaminated groundwater aiming at an aquifer temperature of 20–30 °C. A fermentable substrate (whey) was injected in separate batches. The test was monitored using hydrochemical and molecular tools (qPCR and NGS). The addition of the substrate and increase in temperature resulted in a rapid increase in the abundance of reductive dechlorinators (e.g., Dehalococcoides mccartyi, Dehalobacter sp. and functional genes vcrA and bvcA) and a strong increase in CVOC degradation. On day 34, the CVOC concentrations decreased by 87% to 96% in groundwater from the wells most affected by the heating and substrate. On day 103, the CVOC concentrations were below the LOQ resulting in degradation half-lives of 5 to 6 days. Neither an increase in biomarkers nor a distinct decrease in the CVOC concentrations was observed in a deep well affected by the heating but not by the substrate. NGS analysis detected Chloroflexi dechlorinating genera (Dehalogenimonas and GIF9 and MSBL5 clades) and other genera capable of anaerobic metabolic degradation of CVOCs. Of these, bacteria of the genera Acetobacterium, Desulfomonile, Geobacter, Sulfurospirillum, Methanosarcina and Methanobacterium were stimulated by the substrate and heating. In contrast, groundwater from the deep well (affected by heating only) hosted representatives of aerobic metabolic and aerobic cometabolic CVOC degraders. The test results document that heating of the treated aquifer significantly accelerated the treatment process but only in the case of an abundant substrate.
- ItemTool V: Microbiological Methods for Monitoring nZVI Performance in Groundwater Conditions(Springer, 2020-01-01) Ševců Alena; Dolinová Iva; Cajthaml Tomáš; Steinová Jana; Špánek Roman