Browsing by Author "Kuráň, Pavel"
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- ItemBiodegradation of High Concentrations of Aliphatic Hydrocarbons in Soil from a Petroleum Refinery: Implications for Applicability of New Actinobacterial Strains(MDPI, 2019-11-09) Trögl, Josef; Esuola, Catherine Olwakemi; Kříženecká, Sylvie; Kuráň, Pavel; Seidlová, Lenka; Veronesi-Dáňová, Petra; Popelka, Jan; Babalola, Olubukola Oluranti; Hrabák, Pavel; Czinnerová, Marie; Kakosová, Eva; Ševců, Alena; Tischler, DirkAt present, there is great demand for new resistant and metabolically active strains of biodegrading bacteria capable of degrading high concentrations of petroleum pollutants. In this study, we undertook a series of pot-based biodegradation experiments on soil from a petroleum refinery lagoon heavily polluted with aliphatic hydrocarbons (81.6 ± 2.5 g·kg−1 dry weight) and metals. Periodical bioaugmentation with either a mixture of isolated degraders identified as Bacillus sp. and Ochrobactrum sp. or biostimulation with nutrient medium, singly or in combination, did not produce any significant decrease in hydrocarbons, even after 455 days. Inoculation with Gordonia rubripertincta CWB2 and Rhodococcus erythropolis S43 in iron-limited media, however, resulted in a significant decrease in hydrocarbons 45 days after bioaugmentation. These actinobacterial strains, therefore, show significant potential for bioremediation of such highly polluted soils.
- ItemSample pretreatment in determination of anions in mine waters with high Iron contents(Technická Univerzita v Liberci, 2013) Kuráň, Pavel; Čmelík, Jiří; Pilařová, Vera; Krejčová, Michaela; Janoš, Pavel; Kuráň, Pavel; Čmelík, Jiří; Pilařová, Vera; Krejčová, Michaela; Janoš, PavelReliable analysis of anions in mine waters with high iron and sulfate contents is important for their characterization. However, both traditional and LC methods failed in direct anion analysis. The article describes two sample pretreatments - alkalization and cation exchange. The pretreated water samples were analyzed by potentiometric titration, spectrophotometry, ion chromatography with conductivity detector in suppressed regime and HPLC with diode array detector using indirect UV detection at 260 nm. Both the sample pretreatments enable determination of some anions, the former procedure being more suitable for the purpose. Potentiometric titration and spectrophotometry seem to be the most suitable due to low detection limits for all the selected anions ranging from 0.4 to 1.8 mg l-1. The application of ion chromatography and liquid chromatography with diode array detector for the purpose is also possible. However, due to very high detection limits of samples pretreated by cation exchange (26-121 mg l-1) and low recoveries of samples pretreated by alkalization (<17 %) for all the selected anions, HPLC-DAD is not suitable for analysis of real samples. Validation and analytical characteristics of the methods are given and discussed.