Browsing by Author "Klápšťová, Andrea"

Now showing 1 - 5 of 5
  • Thumbnail Image
    Item
    Impact of Various Sterilization and Disinfection Techniques on Electrospun Poly-epsilon-caprolactone
    (AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2020-04-21) Horáková, Jana; Klíčová, Markéta; Erben, Jakub; Klápšťová, Andrea; Novotný, Vít; Běhálek, Luboš; Chvojka, Jiří
    Electrospun materials made from biodegradable polycaprolactone are used widely in various tissue engineering and regenerative medicine applications because of their morphological similarity to the extracellular matrix. However, the main prerequisite for the use of such materials in clinical practice consists of the selection of the appropriate sterilization technique. This study is devoted to the study of the impact of traditional sterilization and disinfection methods on a nanofibrous polycaprolactone layer constructed by means of the needleless electrospinning technique. It was determined that hydrogen peroxide plasma treatment led to the loss of fibrous morphology and the creation of a foil. However, certain sterilization (ethylene oxide, gamma irradiation, and peracetic acid) and disinfection techniques (ethanol and UV irradiation) were found not to lead to a change in morphology; thus, the study investigates their impact on thermal properties, molecular weight, and interactions with a fibroblast cell line. It was determined that the surface properties that guide cell adhesion and proliferation were affected more than the bulk properties. The highest proliferation rate of fibroblasts seeded on nanofibrous scaffolds was observed with respect to gamma-irradiated polycaprolactone, while the lowest proliferation rate was observed following ethylene oxide sterilization.
  • Thumbnail Image
    Item
    Impact of Various Sterilization and Disinfection Techniques on Electrospun Poly-ϵ-caprolactone
    (American Chemical Society, 2020-01-01) Horáková, Jana; Klíčová, Markéta; Erben, Jakub; Klápšťová, Andrea; Novotný, Vít; Běhálek, Luboš; Chvojka, Jiří
    Electrospun materials made from biodegradable polycaprolactone are used widely in various tissue engineering and regenerative medicine applications because of their morphological similarity to the extracellular matrix. However, the main prerequisite for the use of such materials in clinical practice consists of the selection of the appropriate sterilization technique. This study is devoted to the study of the impact of traditional sterilization and disinfection methods on a nanofibrous polycaprolactone layer constructed by means of the needleless electrospinning technique. It was determined that hydrogen peroxide plasma treatment led to the loss of fibrous morphology and the creation of a foil. However, certain sterilization (ethylene oxide, gamma irradiation, and peracetic acid) and disinfection techniques (ethanol and UV irradiation) were found not to lead to a change in morphology; thus, the study investigates their impact on thermal properties, molecular weight, and interactions with a fibroblast cell line. It was determined that the surface properties that guide cell adhesion and proliferation were affected more than the bulk properties. The highest proliferation rate of fibroblasts seeded on nanofibrous scaffolds was observed with respect to gamma-irradiated polycaprolactone, while the lowest proliferation rate was observed following ethylene oxide sterilization.
  • Thumbnail Image
    Item
    Novel double-layered planar scaffold combining electrospun PCL fibers and PVA hydrogels with high shape integrity and water stability
    (Elsevier B.V., 2020-03-01) Klíčová, Markéta; Klápšťová, Andrea; Chvojka, Jiří; Kopřivová, Barbora; Jenčová, Věra; Horáková, Jana
    Novel double-layered materials with different properties of each side were prepared via needleless electrospinning and compared in terms of morphology, wettability, adhesion and proliferation of mouse fibroblasts. The materials consist of hydrophilic poly(vinyl alcohol) fibers with low (PVA_L) or high (PVA_H) degree of hydrolysis, and hydrophobic poly(ε-caprolactone) (PCL) fibrous layer. Although the PVA_L fibers were fully dissolved following a water exposure, the shape of the scaffold was maintained due to water stable PCL layer. Exposing PVA_H based fibrous layer to water created a hydrogel-like structure with shape defined by the PCL layer. According to the MTT assay, the mouse fibroblasts seeded on the scaffold exhibited the greatest proliferative activity on the PCL fibers. These double-layered scaffolds with different features on each side are very promising for many novel medical applications such as wound dressing or abdominal adhesion prevention.
  • Thumbnail Image
    Item
    Výroba modifikovaných nanokompozitních materiálů kombinací elektrostatického zvlákňování a sprejování
    (Technická Univerzita v Liberci, 2012-01-01) Klápšťová, Andrea; Mikeš, Petr
    Tato bakalářská práce se zabývá výrobou modifikovaných nanokompozitních materiálů kombinací elektrostatického zvlákňování a rozprašování. V teoretické části práce jsou vysvětleny oba pojmy a shrnuty parametry ovlivňující proces elektrostatického rozprašování. Dále jsou zde definovány použité polymery a aktivní látky inkorporované do polymerního obalu a vysvětlen způsob enkapsulování těchto látek. Experimentální část se zabývá popisem použitých chemikálií a laboratorních zařízení, dále způsobem výroby polymerních nanočástic a enkapsulací aktivních látek do polymerního obalu technologií elektrostatického rozprašování. Následně je popsána výroba nanokompozitních materiálů kombinováním obou technologií.
  • Thumbnail Image
    Item
    Využití elektrosprayingu pro cílené dodávání léčiv a funkcionalizaci scaffoldů
    (Technická Univerzita v Liberci, 2014-01-01) Klápšťová, Andrea; Mikeš, Petr
    Diplomová práce se zabývá využitím elektrosprayingu pro cílené dodávání léčiv a funkcionalizaci scaffoldů. V teoretické části práce jsou vysvětleny jednotlivé pojmy, včetně parametrů ovlivňujících proces elektrostatického rozprašování. Dále jsou zde definovány použité polymery, rozpouštědlové systémy a aktivní látky inkorporované do polymerního obalu a vysvětlen princip a způsob enkapsulace těchto látek. Experimentální část se zabývá technologií výroby a optimalizací nanočástic z PVA včetně možností jejich síťování. Dále je zde popsána enkapsulace aktivních látek do polymerního obalu technologií elektrospraying. V závěru je objasněna výroba nanokompozitních materiálů kombinováním obou technologií.