Browsing by Author "Švarcová Tereza"
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- ItemThe effect of ethylene oxide sterilization on electrospun vascular grafts made from biodegradable polyesters(ELSEVIER SCIENCE BV, 2018) Horáková Jana; Mikeš Petr; Šaman Aleš; Jenčová Věra; Klápšťová Andrea; Švarcová Tereza; Ackermann Michal; Novotný Vít; Suchý Tomáš; Lukáš DavidThe study describes the detailed examination of the effect of ethylene oxide sterilization on electrospun scaffolds constructed from biodegradable polyesters. Different fibrous layers fabricated from polycaprolactone (PCL) and a copolymer consisting of polylactide and polycaprolactone (PLCL) were investigated for the determination of their mechanical properties, degradation rates and interaction with fibroblasts. It was discovered that the sterilization procedure influenced the mechanical properties of the electrospun PLCL copolymer scaffold to the greatest extent. No effect of ethylene oxide sterilization on degradation behavior was observed. However, a delayed fibroblast proliferation rate was noticed with concern to the ethylene oxide sterilized samples compared to the ethanol sterilization of the materials.
- ItemElectrospun vascular grafts fabricated from poly(L-lactide-co-epsilon-caprolactone) used as a bypass for the rabbit carotid artery(IOP Publishing, 2018) Horáková Jana; Mikeš Petr; Lukáš David; Šaman Aleš; Jenčová Věra; Klápšťová Andrea; Švarcová Tereza; Ackermann Michal; Novotný Vít; Kaláb Martin; Lonský Vladimír; Bartoš Martin; Rampichova Michala; Litvinec Andrej; Kubíková Tereza; Tomášek Petr; Tonar ZbyněkThe study involved the electrospinning of the copolymer poly(L-lactide-co-ε-caprolactone) (PLCL) into tubular grafts. The subsequent material characterization, including micro-computed tomography analysis, revealed a level of porosity of around 70%, with pore sizes of 9.34 ± 0.19 μm and fiber diameters of 5.58 ± 0.10 μm. Unlike fibrous polycaprolactone, the electrospun PLCL copolymer promoted fibroblast and endothelial cell adhesion and proliferation in vitro. Moreover, the regeneration of the vessel wall was detected following implantation and, after six months, the endothelialization of the lumen and the infiltration of arranged smooth muscle cells producing collagen was observed. However, the degradation rate was found to be accelerated in the rabbit animal model. The study was conducted under conditions that reflected the clinical requirements—the prostheses were sutured in the end-to-side fashion and the long-term end point of prosthesis healing was assessed. The regeneration of the vessel wall in terms of endothelialization, smooth cell infiltration and the presence of collagen fibers was observed after six months in vivo. A part of the grafts failed due to the rapid degradation rate of the PLCL copolymer.