Příprava a analýza modifikovaných polypyrrolových vrstev
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Tato diplomová práce se zabývá přípravou cyklodextrinem (CD) modifikovaných vrstev polypyrrolu (PPy) na polykaprolaktonových (PCL) nanovláknech. V teoretické části práce jsou popsány fyzikální a chemické vlastnosti polypyrrolu, způsoby jeho přípravy, stejně jako jeho interakce s živými organismy. Dále jsou popsány vybrané případy modifikace vláken-ných substrátů polypyrolem. Pozornost je věnována také syntéze konjugátu pyrrolu a -cyklodextrinu, který sloužil jako monomer při přípravě modifikovaných PPy vrstev. V praktické části byla připravena nemodifikovaná PPy vrstva na povrchu PCL nanovláken chemickou oxidativní polymerací. Tato vrstva byla podrobena studiu morfologie jejího po-vrchu skenovací elektronovou mikroskopií. Dále byla na povrchu PCL nanovláken připra-vena vrstva kopolymeru pyrrolu a konjugátu pyrrolu a -cyklodextrinu v molárním poměru 19:1. Za účelem sledování počtu molekul -CD přístupných k tvorbě inkluzních komplexů na povrchu vrstvy bylo navržena řada detekčních molekul, byly diskutovány metody jejich syntézy a analýzy vzniklých produktů. Připravené vrstvy byly testovány na adsorpci proteinů pomocí spektrofotometrického měření koncentrace hovězího sérového albuminu (BSA), označeného fluorescenční molekulou. Modifikovaná PPy vrstva vykázala v tomto testu nej-vyšší hodnoty adsorpce proteinů, stejně jako nejvíce podporovala proliferaci a adhezi 3T3 myších fibroblastů při in vitro testování připravených vrstev.
This diploma thesis is dealing with preparation of cyclodextrin (CD) modified polypyrrole layers on the surface of polycaprolactone (PCL) nanofibers. In the theoretical part of this thesis, physical and chemical properties of polypyrrole are described, as well as methods of its preparation and its interaction with living organisms. Selected examples of polypyrrole modified fiber materials are presented. Synthesis of pyrrole conjugate with -CD, which served as monomer in subsequent preparation of modified PPy layers, is also listed. In ex-perimental part of this thesis preparation of unmodified PPy layer on the surface of PCL nanofibers was performed by chemical oxidative polymerization. Surface morphology of this layer was studied using scanning electron microscopy. Subsequently layer of pyr-role/pyrrole -cyclodextrin copolymer in molar ratio 19:1 was prepared on the surface of PCL nanofibers. Several detection molecules were designed to monitor the number of -CD molecules available to inclusion complex formation. Methods of synthesis of these detection molecules as well as analysis of reaction products were discussed Prepared surface layers were tested on protein adhesion by spectrophotometric measurement of concentration of bovine serum albumin (BSA) conjugated with fluorescent molecule. Layers modified with cyclodextrin showed the best protein adhesion of all samples. During in vitro testing of pre-pared layers by 3T3 mouse fibroblast, cyclodextrin-modified layers promoted cell prolifera-tion and adhesion most of all tested samples.
This diploma thesis is dealing with preparation of cyclodextrin (CD) modified polypyrrole layers on the surface of polycaprolactone (PCL) nanofibers. In the theoretical part of this thesis, physical and chemical properties of polypyrrole are described, as well as methods of its preparation and its interaction with living organisms. Selected examples of polypyrrole modified fiber materials are presented. Synthesis of pyrrole conjugate with -CD, which served as monomer in subsequent preparation of modified PPy layers, is also listed. In ex-perimental part of this thesis preparation of unmodified PPy layer on the surface of PCL nanofibers was performed by chemical oxidative polymerization. Surface morphology of this layer was studied using scanning electron microscopy. Subsequently layer of pyr-role/pyrrole -cyclodextrin copolymer in molar ratio 19:1 was prepared on the surface of PCL nanofibers. Several detection molecules were designed to monitor the number of -CD molecules available to inclusion complex formation. Methods of synthesis of these detection molecules as well as analysis of reaction products were discussed Prepared surface layers were tested on protein adhesion by spectrophotometric measurement of concentration of bovine serum albumin (BSA) conjugated with fluorescent molecule. Layers modified with cyclodextrin showed the best protein adhesion of all samples. During in vitro testing of pre-pared layers by 3T3 mouse fibroblast, cyclodextrin-modified layers promoted cell prolifera-tion and adhesion most of all tested samples.
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Subject(s)
polypyrrol, cyklodextrin, nanovlákna, povrchové vrstvy, tkáňové nosiče, polypyrrole, cyclodextrin, nanofibers, surface layers, tissue scafolds