Browsing by Author "Koláčná, Lucie"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Biochemical and biophysical aspects of collagen nanostructure in the extracellular matrix
    (Technická Univerzita v Liberci, 2007-01-01) Koláčná, Lucie; Bakešová, J.; Varga, Ferdinand; Košťáková Kuželova, Eva; Plánka, Ladislav; Nečas, Alois; Lukáš, David; Amler, Evžen; Pelouch, Václav
    ECM is composed of different collagenous and non-collagenous proteins. Collagen nanofibers play a dominant role in maintaining the biological and structural integrity of various tissues and organs, including bone, skin, tendon, blood vessels, and cartilage. Artificial collagen nanofibers are increasingly significant in numerous tissue engineering applications and seem to be ideal scaffolds for cell growth and proliferation. The modern tissue engineering task is to develop three-dimensional scaffolds of appropriate biological and biomechanical properties, at the same time mimicking the natural extracellular matrix and promoting tissue regeneration. Furthermore, it should be biodegradable, bioresorbable and non-inflammatory, should provide sufficient nutrient supply and have appropriate viscoelasticity and strength. Attributed to collagen features mentioned above, collagen fibers represent an obvious appropriate material for tissue engineering scaffolds. The aim of this minireview is, besides encapsulation of the basic biochemical and biophysical properties of collagen, to summarize the most promising modern methods and technologies for production of collagen nanofibers and scaffolds for artificial tissue development. © 2007 Institute of Physiology, v. v. i., Academy of Sciences of the Czech Republic.
  • Thumbnail Image
    Item
    Composite hyaluronate-type I collagen-fibrin scaffold in the therapy of osteochondral defects in miniature pigs
    (Acad Sciences Czech Republic, Inst Physiology, 2007-01-01) Filová, Eva; Rampichová, Michala; Handl, Milan; Lytvynets, Andriy; Halouzka, Roman; Usvald, Dušan; Hlučílová, Jana; Procházka, Radek; Dezortová, Monika; Rolencová, Eva; Košťáková Kuželová, Eva; Trč, Tomáš; Šťastný, Eduard; Koláčná, Lucie; Hájek, Milan; Motlík, Jan; Amler, Evžen
    The potential of novel scaffold containing sodium hyaluronate, type I collagen, and fibrin was investigated in the regeneration of osteochondral defects in miniature pigs. Both autologous chondrocyte-seeded scaffolds and non-seeded scaffolds were implanted into two defects located in the non-weight-bearing zone of the femoral trochlea (defect A was located more distally and medially, defect B was located more proximally and laterally). Control defects were left untreated. Twelve weeks after the operation, the knees were evaluated in vivo using MRI Six months after the implantation, the defects were analyzed using MRI, histological, and immunohistochernical analysis. In the A defects of chondrocyte-seeded scaffold group, hyaline cartilage and fibrocartilage was formed, containing type II collagen, acidic and neutral glycosaminoglycans while the non-seeded scaffold group was predominantly filled with fibrocartilage. Defects in the control group were predominantly filled with fibrous tissue. Histomorphometric analysis of photomicrographs revealed a significantly higher amount of hyaline cartilage in the cell-seeded scaffold group in A defects than in other groups. Both scaffold groups in A defects showed significantly less fibrous tissue than cell-seeded defects B and the control group. Both histological and MRI analysis proved that the novel composite scaffold has a potential to regenerate osteochondral defects within six months.