Autor | Rachael L. Hardison | |
---|---|---|
Autor | Sarah W. Nelson | |
Autor | Daniela Barriga | |
Autor | Jessica M. Ghere | |
Autor | Gabrielle A. Fenton | |
Autor | Ryan R. James | |
Autor | Michael J. Stewart | |
Autor | Sangdon Lee | |
Autor | M. Worth Calfee | |
Autor | Shawn P. Ryan | |
Autor | Megan W. Howard | |
Typ | Článek v časopise | |
Datum | 2021-12-08 | |
Abstrakt | This study evaluated the efficacy of detergent-based surface cleaning methods against Murine Hepatitis Virus A59 (MHV) as a surrogate coronavirus for SARS-CoV-2. MHV (5% soil load in culture medium or simulated saliva) was inoculated onto four different high-touch materials [stainless steel (SS), Acrylonitrile Butadiene Styrene plastic (ABS), Formica, seat fabric (SF)]. Immediately and two-hours post-inoculation, coupons were cleaned (damp wipe wiping) with and without pre-treatment with detergent solution or 375 ppm hard water. Results identified that physical removal (no pre-treatment) removed >2.3 log10 MHV on ABS, SS, and Formica when surfaces were cleaned immediately. Pre-treatment with detergent or hard water increased effectiveness over wet wiping two-hours post-inoculation; pre-treatment with detergent significantly increased (p ≤ 0.05) removal of MHV in simulated saliva, but not in culture media, over hard water pre-treatment (Formica and ABS). Detergent and hard water cleaning methods were ineffective on SF under all conditions. Overall, efficacy of cleaning methods against coronaviruses are material- and matrix-dependent; pre-wetting surfaces with detergent solutions increased efficacy against coronavirus suspended in simulated saliva. This study provides data highlighting the importance of incorporating a pre-wetting step prior to detergent cleaning and can inform cleaning strategies to reducing coronavirus surface transmission. | |
Přístup | 16. 12. 2021 10:30:05 | |
Publikace | Journal of Occupational and Environmental Hygiene | |
Ročník | 0 | |
Číslo | ja | |
Rozsah | 1-13 | |
Extra | Publisher: Taylor & Francis _eprint: https://doi.org/10.1080/15459624.2021.2015075 PMID: 34878351 | |
ISSN | 1545-9624 | |
Katalog knihovny | Taylor and Francis+NEJM | |
DOI | 10.1080/15459624.2021.2015075 | |
Select in Zotero | zotero://select/library/items/CBKRYJRF | |
Datum přidání | 16. 12. 2021 10:30:05 | |
Upraveno | 16. 12. 2021 12:13:39 |
Autor | M. Varbanov | |
---|---|---|
Autor | I. Bertrand | |
Autor | S. Philippot | |
Autor | C. Retourney | |
Autor | M. Gardette | |
Autor | C. Hartard | |
Autor | H. Jeulin | |
Autor | R. E. Duval | |
Autor | J. -F. Loret | |
Autor | E. Schvoerer | |
Autor | C. Gantzer | |
Typ | Článek v časopise | |
Datum | 2021-11-25 | |
Abstrakt | High concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome have been described in wastewater and sewage sludge. It raises the question of the security of land sludge disposal practices during a pandemic. This study aimed to compare SARS-CoV-2's resistance to the main inactivating factors in sludge treatments, pH and heat, to that of native wastewater somatic coliphages. The latest can be easily used as an indicator of treatment efficiency in the field. The effects of heat treatment and pH on the survival of SARS-CoV-2 and somatic coliphages were investigated in simple media. The T90 value (time required for a 90% reduction in the virus or a 1 × log10 decline) at 50 °C was about 4 min for infectious SARS-CoV-2, and around 133 min for infectious somatic coliphages, with no decrease in SARS-CoV-2 genome. For infectious SARS-CoV-2, a slight decrease (<1 log10 unit) was observed at pH 9 or 10 for 10 min; the decrease was over 5 log10 units at pH 11. However, both SARS-CoV-2 genome and infectious somatic coliphages decreased by less than 1 log10 unit at pH 12. All thermal or pH-based treatments that can remove or significantly reduce infectious somatic coliphages (>4 log10) can be considered efficient treatments for infectious SARS-CoV-2. We concluded that somatic coliphages can be considered highly conservative and easy to use indicators of the inactivation of SARS-CoV-2 during treatments based on heat and alkaline pH. | |
Přístup | 16. 12. 2021 10:57:22 | |
Publikace | Science of The Total Environment | |
Jazyk | en | |
Ročník | 797 | |
Rozsah | 149112 | |
ISSN | 0048-9697 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Science of The Total Environment | |
DOI | 10.1016/j.scitotenv.2021.149112 | |
Select in Zotero | zotero://select/library/items/HFTIGVZ8 | |
Datum přidání | 16. 8. 2021 8:10:49 | |
Upraveno | 16. 12. 2021 14:21:38 |
Autor | Marcilio Jorge Fumagalli | |
---|---|---|
Autor | Carlos Fabiano Capato | |
Autor | Luiza Antunes de Castro-Jorge | |
Autor | William Marciel de Souza | |
Autor | Eurico Arruda | |
Autor | Luiz Tadeu Moraes Figueiredo | |
Typ | Článek v časopise | |
Datum | 2021-11-02 | |
Abstrakt | Viral stability under stress conditions may directly affect viral dissemination, seasonality, and pathogenesis. We exposed airborne viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), mumps virus, coxsackievirus B5, human rhinovirus A16, and respiratory syncytial virus, to different temperatures, UV light exposure time, pH values, and osmotic pressures and measured the remaining viral infectivity. Reduced thermal stability was observed for coxsackievirus B5 at 45 °C, while SARS-CoV-2 demonstrated residual infectivity at 55 °C. UV light exposure was an efficient means of viral inactivation but was less efficient for non-enveloped viruses. Rhinovirus A16 and respiratory syncytial virus demonstrated extreme sensitivity to acid conditions, while SARS-CoV-2, rhinovirus A16, and respiratory syncytial virus were unstable in an alkaline environment. The information obtained in this study will be useful for the development of viral inactivation methods and may be correlated with epidemiological and seasonal viral characteristics. | |
Přístup | 16. 11. 2021 9:21:52 | |
Publikace | Archives of Virology | |
Jazyk | en | |
ISSN | 1432-8798 | |
Katalog knihovny | Springer Link | |
Zkrácený název časopisu | Arch Virol | |
DOI | 10.1007/s00705-021-05293-7 | |
Select in Zotero | zotero://select/library/items/E6K85ICU | |
Datum přidání | 16. 11. 2021 9:21:52 | |
Upraveno | 16. 11. 2021 10:30:13 |
Autor | Y. Suzuki | |
---|---|---|
Autor | T. Hishiki | |
Autor | A. Emi | |
Autor | S. Sakaguchi | |
Autor | R. Itamura | |
Autor | R. Yamamoto | |
Autor | T. Matsuzawa | |
Autor | K. Shimotohno | |
Autor | M. Mizokami | |
Autor | T. Nakano | |
Autor | N. Yamamoto | |
Typ | Článek v časopise | |
Datum | 2021-10-14 | |
Abstrakt | Air spaces and material surfaces in a pathogen-contaminated environment can often be a source of infection to humans, and disinfection has become a common intervention focused on reducing the contamination levels. In this study, we examined the efficacy of SAIW, a unique electrolyzed water with chlorine-free, high pH, high concentration of dissolved hydrogen, and low oxygen reduction potential, for the inactivation of several viruses and bacteria. Infectivity assays revealed that initial viral titers of enveloped and non-enveloped viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus, herpes simplex virus type 1, human coronavirus, feline calicivirus, and canine parvovirus, were reduced by 2.9- to 5.5-log10 within 30 s of SAIW exposure. Similarly, the culturability of three Gram-negative bacteria (Escherichia coli, Salmonella, and Legionella) dropped down by 1.9- to 4.9-log10 within 30 s of SAIW treatment. Mechanistically, treatment with SAIW was found to significantly decrease the binding and subsequent entry efficiencies of SARS-CoV-2 on Vero cells. Finally, we showed that this chlorine-free electrolytic ion water had no acute inhalation toxicity in mice, demonstrating that SAIW holds promise for a safer antiviral and antibacterial disinfectant. © 2021 Elsevier Inc. | |
Publikace | Biochemical and Biophysical Research Communications | |
Ročník | 575 | |
Rozsah | 36-41 | |
Archiv | Scopus | |
Katalog knihovny | Scopus | |
DOI | 10.1016/j.bbrc.2021.08.048 | |
Select in Zotero | zotero://select/library/items/DK8BHMN9 | |
Datum přidání | 14. 10. 2021 13:32:16 | |
Upraveno | 14. 10. 2021 14:18:58 |
Autor | Franco Cicconetti | |
---|---|---|
Autor | Piero Sestili | |
Autor | Valeria Madiai | |
Autor | Maria Cristina Albertini | |
Autor | Luigi Campanella | |
Autor | Sofia Coppari | |
Autor | Daniele Fraternale | |
Autor | Bryan Saunders | |
Autor | Laura Teodori | |
Typ | Článek v časopise | |
Datum | 2021-10-11 | |
Abstrakt | The COVID-19 pandemic and its virus variants continue to pose a serious and long-lasting threat worldwide. To combat the pandemic, the world’s largest COVID-19 vaccination campaign is currently ongoing. As of July 19th 2021, 26.2% of the world population has received at least one dose of a COVID-19 vaccine (1.04 billion), and one billion has been fully vaccinated, with very high vaccination rates in countries like Israel, Malta, and the UEA. Conversely, only 1% of people in low-income countries have received at least one dose with examples of vaccination frequency as low as 0.07% in the Democratic Republic of Congo. It is thus of paramount importance that more research on alternate methods to counter cell infection and propagation is undertaken that could be implemented in low-income countries. Moreover, an adjunctive therapeutic intervention would help to avoid disease exacerbation in high-rate vaccinated countries too. Based on experimental biochemical evidence on viral cell fusion and propagation, herein we identify (i) extracellular pH (epH), (ii) temperature, and (iii) humidity and osmolarity as critical factors. These factors are here in discussed along with their implications on mucus thick layer, proteases, abundance of sialic acid, vascular permeability and exudate/edema. Heated, humidified air containing sodium bicarbonate has long been used in the treatment of certain diseases, and here we argue that warm inhalation of sodium bicarbonate might successfully target these endpoints. Although we highlight the molecular/cellular basis and the signalling pathways to support this intervention, we underscore the need for clinical investigations to encourage further research and clinical trials. In addition, we think that such an approach is also important in light of the high mutation rate of this virus originating from a rapid increase. | |
Publikace | PeerJ | |
Jazyk | English | |
Archiv | Coronavirus Research Database; ProQuest Central; Publicly Available Content Database | |
Místo v archivu | 2580798699 | |
Extra | Place: San Diego Publisher: PeerJ, Inc. | |
DOI | 10.7717/peerj.12227 | |
Select in Zotero | zotero://select/library/items/UD2UWGPY | |
Datum přidání | 14. 10. 2021 13:28:21 | |
Upraveno | 14. 10. 2021 14:20:06 |
Copyright - © 2021 Cicconetti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Poslední aktualizace - 2021-10-12
Autor | M Khalid Ijaz | |
---|---|---|
Autor | Raymond W Nims | |
Autor | Sarah de Szalay | |
Autor | Joseph R Rubino | |
Typ | Článek v časopise | |
Datum | 2021-09-17 | |
Abstrakt | Public Health Agencies worldwide (World Health Organization, United States Centers for Disease Prevention & Control, Chinese Center for Disease Control and Prevention, European Centre for Disease Prevention and Control, etc.) are recommending hand washing with soap and water for preventing the dissemination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. In this review, we have discussed the mechanisms of decontamination by soap and water (involving both removal and inactivation), described the contribution of the various components of formulated soaps to performance as cleansers and to pathogen inactivation, explained why adherence to recommended contact times is critical, evaluated the possible contribution of water temperature to inactivation, discussed the advantages of antimicrobial soaps vs. basic soaps, discussed the differences between use of soap and water vs. alcohol-based hand sanitizers for hand decontamination, and evaluated the limitations and advantages of different methods of drying hands following washing. While the paper emphasizes data applicable to SARS-CoV-2, the topics discussed are germane to most emerging and re-emerging enveloped and non-enveloped viruses and many other pathogen types. | |
Publikace | PeerJ | |
Jazyk | English | |
Archiv | Coronavirus Research Database; ProQuest Central; Publicly Available Content Database | |
Místo v archivu | 2573627448 | |
Extra | Place: San Diego Publisher: PeerJ, Inc. | |
DOI | 10.7717/peerj.12041 | |
Select in Zotero | zotero://select/library/items/4WSKYIFA | |
Datum přidání | 14. 10. 2021 13:28:21 | |
Upraveno | 14. 10. 2021 14:19:44 |
Copyright - © 2021 Ijaz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Název - World Health Organization; Centers for Disease Control & Prevention--CDC
Poslední aktualizace - 2021-09-18
SubjectsTermNotLitGenreText - United States--US
Autor | Alba Cano-Vicent | |
---|---|---|
Autor | Alberto Tunon-Molina | |
Autor | Miguel Marti | |
Autor | Yukiko Muramoto | |
Autor | Takeshi Noda | |
Autor | Kazuo Takayama | |
Autor | Angel Serrano-Aroca | |
Typ | Článek v časopise | |
Datum | 2021-09-14 | |
Abstrakt | Infection prevention clothing is becoming an essential protective tool in the current pandemic, especially because now we know that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can easily infect humans in poorly ventilated indoor spaces. However, commercial infection prevention clothing is made of fabrics that are not capable of inactivating the virus. Therefore, viral infections of symptomatic and asymptomatic individuals wearing protective clothing such as masks can occur through aerosol transmission or by contact with the contaminated surfaces of the masks, which are suspected as an increasing source of highly infectious biological waste. Herein, we report an easy fabrication method of a novel antiviral non-woven fabric containing polymer filaments that were coated with solidified hand soap. This extra protective fabric is capable of inactivating enveloped viruses such as SARS-CoV-2 and phage F6 within 1 min of contact. In this study, this antiviral fabric was used to fabricate an antiviral face mask and did not show any cytotoxic effect in human keratinocyte HaCaT cells. Furthermore, this antiviral non-woven fabric could be used for the fabrication of other infection prevention clothing such as caps, scrubs, shirts, trousers, disposable gowns, overalls, hoods, aprons, and shoe covers. Therefore, this low-cost technology could provide a wide range of infection-protective tools to combat COVID-19 and future pandemics in developed and underdeveloped countries. | |
Přístup | 14. 10. 2021 13:36:45 | |
Publikace | Acs Omega | |
Krátký název | Antiviral Face Mask Functionalized with Solidified Hand Soap | |
Jazyk | English | |
Ročník | 6 | |
Číslo | 36 | |
Rozsah | 23495-23503 | |
Extra | Place: Washington Publisher: Amer Chemical Soc WOS:000696531700049 | |
ISSN | 2470-1343 | |
Katalog knihovny | Web of Science Nextgen | |
Zkrácený název časopisu | ACS Omega | |
DOI | 10.1021/acsomega.1c03511 | |
Select in Zotero | zotero://select/library/items/T2G8YZRE | |
Datum přidání | 14. 10. 2021 13:36:45 | |
Upraveno | 14. 10. 2021 14:19:16 |
Autor | Yusuf Oloruntoyin AYIPO | |
---|---|---|
Autor | Sani Najib YAHAYA | |
Autor | Halimah Funmilayo BABAMALE | |
Autor | Iqrar AHMAD | |
Autor | Harun PATEL | |
Autor | Mohd Nizam MORDI | |
Typ | Článek v časopise | |
Datum | 2021-08-30 | |
Abstrakt | The nsp3 macrodomain is implicated in the viral replication, pathogenesis and host immune responses through the removal of ADP-ribosylation sites during infections of coronaviruses including the SARS-CoV-2. It has ever been modulated by macromolecules including the ADP-ribose until Ni and co-workers recently reported its inhibition and plasticity enhancement unprecedentedly by remdesivir metabolite, GS-441524, creating an opportunity for investigating other biodiverse small molecules such as β-Carboline (βC) alkaloids. In this study, 1497 βC analogues from the HiT2LEAD chemical database were screened, using computational approaches of Glide XP docking, molecular dynamics simulation and pk-CSM ADMET predictions. Selectively, βC ligands, 129, 584, 1303 and 1323 demonstrated higher binding affinities to the receptor, indicated by XP docking scores of –10.72, –10.01, –9.63 and –9.48 kcal/ mol respectively than remdesivir and GS-441524 with –4.68 and –9.41 kcal/mol respectively. Consistently, their binding free energies were –36.07, –23.77, –24.07 and –17.76 kcal/mol respectively, while remdesivir and GS-441524 showed –21.22 and –24.20 kcal/mol respectively. Interestingly, the selected βC ligands displayed better stability and flexibility for enhancing the plasticity of the receptor than GS-441524, especially 129 and 1303. Their predicted ADMET parameters favour druggability and low expressions for toxicity. Thus, they are recommended as promising adjuvant/standalone anti-SARS-CoV-2 candidates for further study. | |
Publikace | Turkish Journal of Biology | |
Ročník | 45 | |
Rozsah | 503-517 | |
ISSN | 1300-0152 | |
Zkrácený název časopisu | Turk J Biol | |
DOI | 10.3906/biy-2106-64 | |
Select in Zotero | zotero://select/library/items/7R5EGLRD | |
Datum přidání | 16. 11. 2021 9:34:40 | |
Upraveno | 16. 11. 2021 9:40:35 |
Autor | Miriam Simon | |
---|---|---|
Autor | Michael Veit | |
Autor | Klaus Osterrieder | |
Autor | Michael Gradzielski | |
Typ | Článek v časopise | |
Datum | 2021-08-16 | |
Abstrakt | We provide here a general view on the interactions of surfactants with viruses, with a particular emphasis on how such interactions can be controlled and employed for inhibiting the infectivity of enveloped viruses, including coronaviruses. The aim is to provide to interested scientists from different fields, including chemistry, physics, biochemistry, and medicine, an overview of the basic properties of surfactants and (corona)viruses, which are relevant to understanding the interactions between the two. Various types of interactions between surfactant and virus are important, and they act on different components of a virus such as the lipid envelope, membrane (envelope) proteins and nucleocapsid proteins. Accordingly, this cannot be a detailed account of all relevant aspects but instead a summary that bridges between the different disciplines. We describe concepts and cover a selection of the relevant literature as an incentive for diving deeper into the relevant material. Our focus is on more recent developments around the COVID-19 pandemic caused by SARS-CoV-2, applications of surfactants against the virus, and on the potential future use of surfactants for pandemic relief. We also cover the most important aspects of the historical development of using surfactants in combatting virus infections. We conclude that surfactants are already playing very important roles in various directions of defence against viruses, either directly, as in disinfection, or as carrier components of drug delivery systems for prophylaxis or treatment. By designing tailor-made surfactants, and consequently, advanced formulations, one can expect more and more effective use of surfactants, either directly as antiviral compounds or as part of more complex formulations. | |
Přístup | 16. 8. 2021 8:23:32 | |
Publikace | Current Opinion in Colloid & Interface Science | |
Jazyk | en | |
Ročník | 55 | |
Rozsah | 101479 | |
ISSN | 1359-0294 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Current Opinion in Colloid & Interface Science | |
DOI | 10.1016/j.cocis.2021.101479 | |
Select in Zotero | zotero://select/library/items/VGT923YE | |
Datum přidání | 16. 8. 2021 8:23:32 | |
Upraveno | 16. 8. 2021 14:18:51 |
Autor | Stephen H. Bell | |
---|---|---|
Autor | Derek J. Fairley | |
Autor | Hannele Kettunen | |
Autor | Juhani Vuorenmaa | |
Autor | Juha Orte | |
Autor | Connor G. G. Bamford | |
Autor | John W. McGrath | |
Typ | Zpráva | |
Datum | 2021-07-19 | |
Abstrakt | Chemical methods of virus inactivation are used routinely to prevent viral transmission in both a personal hygiene capacity but also in at-risk environments like hospitals. Several ‘virucidal’ products exist, including hand soaps, gels and surface disinfectants. Resin acids, which can be derived from Tall oil produced from trees, have been shown to exhibit anti-bacterial activity. However, whether these products or their derivatives have virucidal activity is unknown. Here, we assessed the capacity of Rosin soap to inactivate a panel of pathogenic mammalian viruses in vitro. We show that Rosin soap can inactivate the human enveloped viruses: influenza A virus (IAV), respiratory syncytial virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For IAV, rosin soap could provide a 100,000-fold reduction in infectivity. However, Rosin soap failed to affect the non-enveloped encephalomyocarditis virus (EMCV). The inhibitory effect of Rosin soap against IAV infectivity was dependent on its concentration but not dependent on incubation time nor temperature. Together, we demonstrate a novel chemical inactivation method against enveloped viruses, which could be of use in preventing virus infections in certain settings. Importance Viruses remain a significant cause of human disease and death, most notably illustrated through the current Covid-19 pandemic. Control of virus infection continues to pose a significant global health challenge to the human population. Viruses can spread through multiple routes, including via environmental and surface contamination where viruses can remain infectious for days. Methods to inactivate viruses on such surfaces may help mitigate infection. Here we present evidence identifying a novel ‘virucidal’ product in Rosin soap, which is produced from Tall oil from coniferous trees. Rosin soap was able to rapidly and potently inactivate influenza virus and other enveloped viruses. | |
Přístup | 16. 8. 2021 8:01:08 | |
URL | https://www.biorxiv.org/content/10.1101/2021.07.19.452918v1 | |
Jazyk | en | |
Rozsah | 2021.07.19.452918 | |
Práva | © 2021, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Extra | Company: Cold Spring Harbor Laboratory DOI: 10.1101/2021.07.19.452918 Distributor: Cold Spring Harbor Laboratory Label: Cold Spring Harbor Laboratory Section: New Results Type: article | |
Katalog knihovny | bioRxiv | |
Select in Zotero | zotero://select/library/items/INGQ3S3I | |
Datum přidání | 16. 8. 2021 8:01:08 | |
Upraveno | 16. 8. 2021 8:01:08 |
Autor | Mejdi Snoussi | |
---|---|---|
Autor | Alaeddine Redissi | |
Autor | Amor Mosbah | |
Autor | Vincenzo De Feo | |
Autor | Mohd Adnan | |
Autor | Kaïss Aouadi | |
Autor | Mousa Alreshidi | |
Autor | Mitesh Patel | |
Autor | Adel Kadri | |
Autor | Emira Noumi | |
Typ | Článek v časopise | |
Datum | 2021-07-13 | |
Abstrakt | The main objective of this study is to find out the anti-SARS-CoV-2 potential of emetine by using molecular docking and dynamic simulation approaches. Interestingly, molecular docking studies suggest that Emetine showed significant binding affinity toward Nsp15 (-10.8kcal/mol) followed by Nsp12 (-9.5kcal/mol), RNA-dependent RNA polymerase, RdRp (-9.5kcal/mol), Nsp16 (-9.4kcal/mol), Nsp10 (-9.2kcal/mol), Papain-like protein (-9.0kcal/mol), Nsp13 (-9.0kcal/mol), Nsp14 (-8.9kcal/mol) and Spike Protein Receptor Domain (-8.8kcal/mol) and chymotrypsin-like protease, 3CLpro (-8.5kcal/mol), respectively, which are essential for viral infection and replication. In addition, molecular dynamic simulation (MD) was also performed for 140ns to explore the stability behavior of the main targets and inhibitor complexes as well as the binding mechanics of the ligand to the target proteins. The obtained MD results followed by absolute binding energy calculation confirm that the binding of emetine at the level of the various receptors is more stable. The complex EmetineNSP15, mechanistically was stabilized as follows: Emetine first binds to the monomer, after, binds to the second inducing the formation of a dimer which in turn leading to the formation of complex that simulation stabilizes it at a value less than 5Å. Overall, supported by the powerful and good pharmacokinetic data of Emetine, our findings with clinical trials may be helpful to confirm that Emetine could be promoted in the prevention and eradication of COVID-19 by reducing the severity in the infected persons and therefore can open possible new strategies for drug repositioning. Communicated by Ramaswamy H. Sarma. © 2021 Informa UK Limited, trading as Taylor & Francis Group. | |
Přístup | 16. 8. 2021 8:03:24 | |
Publikace | Journal of Biomolecular Structure and Dynamics | |
Krátký název | Emetine, a potent alkaloid for the treatment of SARS-CoV-2 targeting papain-like protease and non-structural proteins | |
Jazyk | en | |
Rozsah | 1-14 | |
ISSN | 1538-0254 | |
Katalog knihovny | DOI.org (Crossref) | |
Zkrácený název časopisu | Journal of Biomolecular Structure and Dynamics | |
DOI | 10.1080/07391102.2021.1946715 | |
Select in Zotero | zotero://select/library/items/4D5F9CZ9 | |
Datum přidání | 16. 8. 2021 8:03:02 | |
Upraveno | 16. 8. 2021 14:19:40 |
Autor | Bin Wang | |
---|---|---|
Typ | Článek v časopise | |
Datum | 2021-06-01 | |
Abstrakt | The frequent outbreaks of life-threatening RNA viruses, including the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), pose tremendous challenges to humanity. The author proposes that creating a more alkaline extracellular environment that is unsuitable for the fusion between the envelope of SARS-CoV-2 and the host cell membrane is a promising method to prevent the entry of coronaviruses into human cells. The alkaline environment could be achieved by exposing the general public to water-clustered negative air ions (NAIs), both indoors and outdoors, to induce a gradual increase in the pH of the human body. Previous studies have demonstrated that there are no harmful effects of high-concentration NAIs on human health. | |
Přístup | 14. 10. 2021 13:53:05 | |
Publikace | Genome | |
Ročník | 64 | |
Číslo | 6 | |
Rozsah | 595-598 | |
Extra | Publisher: NRC Research Press | |
ISSN | 0831-2796 | |
Katalog knihovny | cdnsciencepub.com (Atypon) | |
DOI | 10.1139/gen-2020-0167 | |
Select in Zotero | zotero://select/library/items/FE7RW4X3 | |
Datum přidání | 14. 10. 2021 13:53:05 | |
Upraveno | 14. 10. 2021 14:19:09 |
Autor | Ralf Lucassen | |
---|---|---|
Autor | Mirko Weide | |
Autor | Dirk Bockmühl | |
Typ | Článek v časopise | |
Datum | 2021-06 | |
Abstrakt | Not only since SARS-CoV-2, have transmission routes of viruses been of interest. Noroviruses e.g., can be transmitted via smear infection, are relatively stable in the environment and very resistant to chemical disinfection. Some studies determined the virucidal efficacy of laundering processes, but few studies focused on the virucidal efficacy of dishwashing processes. Here, especially consumer related conditions are of interest. Households for example are a hotspot of norovirus infection and thus a sufficient reduction of these and other viruses from dishes must be insured to avoid an infection via this route. The likelihood of such an event should not be underestimated, since it was shown that the washing machine can be a reservoir for the transmission of extended spectrum beta-lactamase producing bacteria in newborns. Although viruses do not replicate in these devices a transmission via contaminated cutlery e.g., cannot be excluded. Using a consumer related approach to determine the virucidal efficacy of dishwashers, we found a combination of a bleach containing dishwasher detergent, a cleaning temperature of 45 °C for 45 min and a rinsing temperature of 50 °C, to be sufficient to reduces viral titer of bovine corona virus, murine norovirus and modified vaccinia virus by 4.8, 4.2 and 3.8 logarithmic stages respectively. | |
Přístup | 14. 10. 2021 13:58:10 | |
Publikace | Microbiology Research | |
Jazyk | en | |
Ročník | 12 | |
Číslo | 2 | |
Rozsah | 395-402 | |
Práva | http://creativecommons.org/licenses/by/3.0/ | |
Extra | Number: 2 Publisher: Multidisciplinary Digital Publishing Institute | |
Katalog knihovny | www.mdpi.com | |
DOI | 10.3390/microbiolres12020027 | |
Select in Zotero | zotero://select/library/items/CD428G8R | |
Datum přidání | 14. 10. 2021 13:58:10 | |
Upraveno | 14. 10. 2021 14:19:04 |
Autor | Toni Luise Meister | |
---|---|---|
Autor | Jill Fortmann | |
Autor | Daniel Todt | |
Autor | Natalie Heinen | |
Autor | Alfred Ludwig | |
Autor | Yannick Brüggemann | |
Autor | Carina Elsner | |
Autor | Ulf Dittmer | |
Autor | Joerg Steinmann | |
Autor | Stephanie Pfaender | |
Autor | Eike Steinmann | |
Typ | Článek v časopise | |
Datum | 2021-05-16 | |
Abstrakt | The emergence of novel SARS-CoV-2 B.1.1.7 and B.1.351 variants of concern with increased transmission dynamics has raised questions regarding stability and disinfection of these viruses. In this study, we analyzed surface stability and disinfection of the currently circulating SARS-CoV-2 variants B.1.1.7 and B.1.351 compared to the wild type. Treatment with heat, soap and ethanol revealed similar inactivation profiles indicative of a comparable susceptibility towards disinfection. Furthermore, we observed comparable surface stability on steel, silver, copper and face masks. Overall, our data support the application of currently recommended hygiene concepts to minimize the risk of B.1.1.7 and B.1.351 transmission. | |
Publikace | The Journal of Infectious Diseases | |
Jazyk | en | |
Číslo | jiab260 | |
Extra | Place: United States Publisher: Oxford University Press | |
ISSN | 1537-6613 | |
Zkrácený název časopisu | The Journal of Infectious Diseases | |
DOI | 10.1093/infdis/jiab260 | |
Select in Zotero | zotero://select/library/items/HZ5ALLGC | |
Datum přidání | 7. 7. 2021 15:17:18 | |
Upraveno | 7. 7. 2021 15:35:06 |
Autor | Rajesh Ghosh | |
---|---|---|
Autor | Ayon Chakraborty | |
Autor | Ashis Biswas | |
Autor | Snehasis Chowdhuri | |
Typ | Článek v časopise | |
Datum | 2021-04 | |
Abstrakt | Among J. adhatoda six alkaloids, anisotine interacts strongly with SARS CoV-2 Mpro. • Anisotine interacts with both the catalytic residues (His41 and Cys145) of Mpro. • The stability of Mpro-anisotine complex is assessed using 100 ns MD simulation. • Anisotine is proposed to inhibit the proteolytic activity of SARS CoV-2 Mpro. The COVID-19 pandemic, caused by SARS CoV-2, is responsible for millions of death worldwide. No approved/proper therapeutics is currently available which can effectively combat this outbreak. Several attempts have been undertaken in the search of effective drugs to control the spread of SARS CoV-2 infection. The main protease (Mpro), key component for the cleavage of the viral polyprotein, is considered to be one of the important drug targets for treating COVID-19. Various phytochemicals, including polyphenols and alkaloids, have been proposed as potent inhibitors of Mpro. The alkaloids from leaf extracts of Justicia adhatoda have also been reported to possess anti-viral activity. But whether these alkaloids exhibit any inhibitory effect on SARS CoV-2 Mpro is far from clear. To explore this in detail, we have adopted computational approaches. Justicia adhatoda alkaloids possessing proper drug-likeness properties and two anti-HIV drugs (lopinavir and darunavir; having binding affinity -7.3 to -7.4 kcal/mol) were docked against SARS CoV-2 Mpro to study their binding properties. Only one alkaloid (anisotine) had interaction with both the catalytic residues (His41 and Cys145) of Mpro and exhibited good binding affinity (-7.9 kcal/mol). Molecular dynamic simulations (100 ns) revealed that Mpro-anisotine complex is more stable, conformationally less fluctuated; slightly less compact and marginally expanded than Mpro-darunavir/lopinavir complex. Even the number of intermolecular H-bonds and MM-GBSA analysis suggested that anisotine is a more potent Mpro inhibitor than the two previously recommended antiviral drugs (lopinavir and darunavir) and may evolve as a promising anti-COVID-19 drug if proven in animal experiments and on patients. | |
Přístup | 7. 7. 2021 15:02:02 | |
Publikace | Journal of Molecular Structure | |
Krátký název | Identification of alkaloids from Justicia adhatoda as potent SARS CoV-2 main protease inhibitors | |
Jazyk | en | |
Ročník | 1229 | |
Rozsah | 129489 | |
ISSN | 0022-2860 | |
Katalog knihovny | DOI.org (Crossref) | |
Zkrácený název časopisu | Journal of Molecular Structure | |
DOI | 10.1016/j.molstruc.2020.129489 | |
Select in Zotero | zotero://select/library/items/RM5BVEVU | |
Datum přidání | 7. 7. 2021 15:02:02 | |
Upraveno | 7. 7. 2021 15:03:06 |
Autor | Avinash Eknath Gawade | |
---|---|---|
Autor | S. R. Bale | |
Typ | Článek v časopise | |
Datum | 2021-02-17 | |
Abstrakt | Novel Coronavirus (2019-nCoV) is a causative agent of COVID-19. The perilous pandemic caused by 2019-nCoV erupted in late 2019 in China has blanketed the whole world and continues to do so. The incidence of the disease, its morbidity, and mortality is high. Strategic planning to contain a virus, fast track research for prophylactics and therapeutics are underway. Food is one of the components of complementary and alternative medicine of the cultures all over the world. Routine diet and its management which is an easily manageable affair will also play crucial to improve an individual’s resistance to infection. In recent years alkaline diet has secured a large number of proponents and gained popularity among the scientific community as well. Its significance for the treatment of many non-communicable diseases has been recognized. The diet or food component whose net potential renal acid load (PRAL) is low is defined as an alkaline diet. We hypothesize that the alkaline diet could significantly boost an individual’s physiological resistance and immunity against 2019-nCoV by targeting endosomal pH which plays an important role in viral pathogenesis. Hydroxychloroquine, a therapeutic drug that is being used at present for COVID-19 treatment also targets endosomal pH to viral prevent infection. In the present article, we have discussed the significance of diet in traditional medicine, pH homeostasis, the role of pH in 2019-nCoV pathogenesis and the possibilities of how the alkaline diet could interfere with the initial stages of infection. Clinical evidences of the use of the alkaline diet for the treatment of infectious and non-infectious diseases have been reviewed. Further, technical details of the alkaline diet, its correlation to Ayurvedic dietetics have been discussed. Though the current article emphasizes the management of the COVID-19 in the light of pandemic, the approach holds grounds for the other respiratory tract viral pathogens too whose pathogenesis is similar to 2019-nCoV. Future experimental studies to establish such correlation are warranted. Adopting the food which has low dietary acid load (alkaline diet) will aid in boosting the physiological resistance resulting in improved health conditions to fight against 2019-nCoV. | |
Přístup | 12. 4. 2021 9:25:24 | |
URL | http://14.139.47.23/index.php/IJTK/article/view/37864 | |
Publikace | Indian Journal of Traditional Knowledge (IJTK) | |
Krátký název | A nutritional intervention against COVID-19 | |
Jazyk | en | |
Ročník | 19 | |
Číslo | 0 | |
Rozsah | S 158-S 163 | |
Extra | Number: 0 | |
ISSN | 0975-1068 | |
Katalog knihovny | 14.139.47.23 | |
Select in Zotero | zotero://select/library/items/MEFVCP8U | |
Datum přidání | 12. 4. 2021 9:25:24 | |
Upraveno | 4. 5. 2021 15:34:29 |
Autor | Davide Mileto | |
---|---|---|
Autor | Alessandro Mancon | |
Autor | Federica Staurenghi | |
Autor | Alberto Rizzo | |
Autor | Stefano Econdi | |
Autor | Maria Rita Gismondo | |
Autor | Matteo Guidotti | |
Typ | Článek v časopise | |
Datum | 2021-02-16 | |
Abstrakt | A diluted 3% w/w hydrogen peroxide solution acidified to pH 2.5 by adding citric acid inactivated SARS-CoV-2 virus by more than 4 orders of magnitude in 5 min. After a contact time of 15 min, no viral replication was detected. Aqueous solutions of sodium percarbonate inactivated coronavirus by >3 log10 diminution in 15 min. Conversely, H2O2 solutions with no additives displayed a scarce virucidal activity (1.1 log10 diminution in 5 min), confirming that a pH-modifying ingredient is necessary to have a H2O2-based disinfectant active against the novel coronavirus. | |
Přístup | 12. 4. 2021 9:23:05 | |
Publikace | ACS Chemical Health & Safety | |
Krátký název | Inactivation of SARS-CoV-2 in the Liquid Phase | |
Extra | Publisher: American Chemical Society | |
Katalog knihovny | ACS Publications | |
Zkrácený název časopisu | ACS Chem. Health Saf. | |
DOI | 10.1021/acs.chas.0c00095 | |
Select in Zotero | zotero://select/library/items/BJSD88MY | |
Datum přidání | 12. 4. 2021 9:23:05 | |
Upraveno | 12. 4. 2021 9:23:27 |
Autor | T. A. Cutts | |
---|---|---|
Autor | R. W. Nims | |
Autor | S. S. Theriault | |
Autor | E. Bruning | |
Autor | J. R. Rubino | |
Autor | M. K. Ijaz | |
Typ | Článek v časopise | |
Datum | 2021-02-10 | |
Abstrakt | Background Hand washing is an important targeted hygiene intervention for limiting the spread of infectious agents, including the Ebola virus, which continues to re-emerge. We have assessed the virucidal efficacy of a commercially available liquid hand wash product (LHW) for inactivating Ebola virus. Methods The ASTM E1052-11 Standard was used to evaluate the efficacy of an LHW containing the microbicidal active salicylic acid for inactivating Ebola virus - Makona variant suspended in an organic load. Three concentrations (12.5%, 25%, 50%) of three lots of LHW prepared in 440 ppm hard water were evaluated at room temperature for 20, 30, and 60 s contact time. Results A 25% solution of the LHW caused 4.5 log10 and 4.8 log10 reduction in Ebola virus titer within 20 and 30 s, respectively. The efficacy of a 12.5% LHW solution was lower (1.9 and 2.0 log10 reduction in titer within 20 and 30 s, respectively). The efficacy of the 50% LHW solution could not be measured, due to inability to sufficiently neutralize the LHW at the end of exposure. Conclusion These results suggest the potential utility of an appropriately formulated liquid hand wash agent during Ebola virus disease outbreaks for use within healthcare, community, and home settings. Such an LHW should also be effective against other enveloped viruses, such as the pandemic coronavirus SARS-CoV-2. | |
Přístup | 10. 2. 2021 9:55:09 | |
Publikace | Infection Prevention in Practice | |
Krátký název | Hand hygiene | |
Jazyk | en | |
Ročník | 3 | |
Číslo | 1 | |
Rozsah | 100122 | |
ISSN | 2590-0889 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Infection Prevention in Practice | |
DOI | 10.1016/j.infpip.2021.100122 | |
Select in Zotero | zotero://select/library/items/TC5XJM22 | |
Datum přidání | 10. 2. 2021 9:55:09 | |
Upraveno | 5. 3. 2021 10:32:53 |
Autor | E. Feresin | |
---|---|---|
Autor | M. Kondratiuk | |
Autor | J. Nowack | |
Autor | L. Gooßen | |
Typ | Článek v časopise | |
Datum | 2021-02 | |
Abstrakt | Citizens can adopt behavioural precautions against the SARS-CoV-2 virus. Hand hygiene practices that require water and soap or alcohol-water solutions are easy to perform, highly effective preventive measure against the virus. A closer look at the processes happening at molecular level during hand washing/sanitizing reveals the importance of solvation science. In both practices, solvation effects have peculiar and pivotal consequences, and the role of the water solvent is difficult to overestimate: Water assists soap molecules that tear apart the viruś membrane; water mediates and assist the alcohol in inactivating the virus. An understanding of solvation processes is necessary in order to grasp why disinfectants only have to be effective outside but not inside the body. Besides, the principle “more is better” does not apply to the use of soap nor to the alcohol content in a disinfectant. Instead, the right mixture of components ensures ideal effectiveness while providing good skin care. © 2021, Wiley-VCH Verlag. All rights reserved. | |
Publikace | Chemie in Unserer Zeit | |
Krátký název | Handreinigung auf molekularer Ebene – Die Rolle der Solvatation: Das Coronavirus auf einfache Weise zerstören | |
Jazyk | German | |
Ročník | 55 | |
Číslo | 1 | |
Rozsah | 28-37 | |
Archiv | Scopus | |
ISSN | 0009-2851 | |
Katalog knihovny | Scopus | |
Zkrácený název časopisu | Chem. Unserer Zeit | |
DOI | 10.1002/ciuz.202000083 | |
Select in Zotero | zotero://select/library/items/5CCNUM4V | |
Datum přidání | 7. 7. 2021 14:49:20 | |
Upraveno | 16. 8. 2021 14:20:05 |
Autor | Ézio R. A. de Sá | |
---|---|---|
Autor | Allan N. Costa | |
Autor | Rayla K. M. Costa | |
Autor | Janilson L. Souza | |
Autor | Ricardo M. Ramos | |
Autor | Francisco das C. A. Lima | |
Typ | Článek v časopise | |
Datum | 2021-01-12 | |
Abstrakt | The disease outbreak caused by SARS-CoV-2 continues to rise worldwide, even in countries which have considered it controlled. As new cases appear daily, infecting millions of people and causing thousands of deaths, the current in silico study aims to investigate the imidazolic alkaloids of the species Pilocarpus microphyllus (Jaborandi) as a potential inhibitory activity against the Mpro protease from SARS-CoV-2, since it plays a fundamental role in the processing of polyproteins that are translated from viral RNA. Jaborandi is distributed in some Brazilian biomes, being easily identified, yet little researched, with proven anti-inflammatory, contraceptive, anti-diabetic and gastroprotective activities. In this work, DFT calculation of thermodynamic properties, electrostatic potential surface, frontier molecular orbitals and descriptors of chemical reactivity of imidazolic alkaloids were associated with the use of molecular docking techniques, molecular dynamics and ADMET predictions. One can verify a good reactivity chemistry and energetic stability of epiisopiloturine, epiisopilosine, isopilosine and e pilosine with some residues of amino acids present in the active site of the main protease of COVID-19. In this sense, the results point out to the imidazolic alkaloids of Jaborandi as promising targets for in vitro and in vivo tests, as possible candidates for inhibitors of the enzyme Mpro. | |
Přístup | 14. 1. 2021 13:31:51 | |
Publikace | Molecular Simulation | |
Ročník | 47 | |
Číslo | 1 | |
Rozsah | 1-14 | |
Extra | Publisher: Taylor & Francis _eprint: https://www.tandfonline.com/doi/pdf/10.1080/08927022.2021.1873321 | |
ISSN | 0892-7022 | |
Katalog knihovny | Taylor and Francis+NEJM | |
DOI | 10.1080/08927022.2021.1873321 | |
Select in Zotero | zotero://select/library/items/UAA5A8B3 | |
Datum přidání | 14. 1. 2021 13:31:51 | |
Upraveno | 16. 8. 2021 14:08:33 |
Autor | Yetkin Zeki Yilmaz | |
---|---|---|
Autor | Begum Bahar Yilmaz | |
Autor | Yusuf Emre Ozdemir | |
Autor | Bekir Sami Kocazeybek | |
Autor | Ridvan Karaali | |
Autor | Doğan Çakan | |
Autor | Hasan Ahmet Ozdogan | |
Autor | Aysegul Batioglu-Karaaltin | |
Typ | Článek v časopise | |
Datum | 2021 | |
Abstrakt | Objective The causative agent of COVID-19 is a novel member of coronaviridaes, SARS-CoV-2. It has been reported that the spike (S) protein of SARS-CoV-2 is responsible of infectivity. The S protein is demonstrated to be inactivated under environmental condition, such as hypertonicity and alkaline pH. The aim of the study was to investigate the effect of hypertonic alkaline nasal irrigation (HANI) on SARS-CoV-2. Methods Sixty patients divided into two groups. The patients in Group 1 used hydroxychloroquine (HCQ), and the patients in Group 2 used HCQ and HANI. Nasopharyngeal samples were collected at the beginning, on 3rd and 7th day of the PCR test positivity. The nasopharyngeal viral load (NVL) changes analyzed with quantitative PCR. Results NVL decrease in weekly period was statistically significant for both groups, when the difference between NVL day 0 and 3rd in Group 1 and NVL difference between day 0 and 3rd in Group 2 were compared. The difference between Groups 1 and 2 in terms of NVL change was statistically significant (P < 0.05). Conclusion We demonstrated a significant decrease in nasopharyngeal SARS-CoV-2 load with HANI solution and suggest that HANI may be promising modality for the COVID-19 treatment. Level of evidence IB | |
Přístup | 16. 12. 2021 10:14:32 | |
Publikace | Laryngoscope Investigative Otolaryngology | |
Jazyk | en | |
Ročník | 6 | |
Číslo | 6 | |
Rozsah | 1240-1247 | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/lio2.686 | |
ISSN | 2378-8038 | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1002/lio2.686 | |
Select in Zotero | zotero://select/library/items/M4X5GV5J | |
Datum přidání | 16. 12. 2021 10:14:32 | |
Upraveno | 16. 12. 2021 10:14:51 |
Autor | M. Khalid Ijaz | |
---|---|---|
Autor | Raymond Nims | |
Autor | Sarah de Szalay | |
Autor | Joseph R. Rubino | |
Typ | Článek v časopise | |
Datum | 2020-12-18 | |
Abstrakt | Public Health Agencies worldwide (World Health Organization, U.S. Centers for Disease Prevention and Control, Chinese Center for Disease Control and Prevention, European Centre for Disease Prevention and Control, etc.) are recommending hand washing with soap and water for preventing the dissemination of SARS-CoV-2 infections. In this review, we have discussed the mechanism of decontamination by soap and water (involving both removal and inactivation), described the contribution of the various components of formulated soaps to performance as cleansers and to pathogen inactivation, explained why adherence to recommended contact times is critical, evaluated the possible contribution of water temperature to inactivation, discussed the advantages of antimicrobial soaps versus basic soaps, discussed the differences between use of soap and water versus alcohol-based hand sanitizers for hand decontamination, and evaluated the limitations and advantages of different methods of drying hands following washing. While the paper emphasizes data applicable to SARS-CoV-2, the topics discussed are germane to most emerging and re-emerging enveloped and non-enveloped viruses and other pathogens. | |
Přístup | 14. 1. 2021 13:47:30 | |
Krátký název | Soap, Water, and SARS-CoV-2 | |
Extra | DOI: | |
Katalog knihovny | OSF Preprints | |
DOI | 10.31219/osf.io/eazct | |
Select in Zotero | zotero://select/library/items/4X6IATJ7 | |
Datum přidání | 14. 1. 2021 13:47:30 | |
Upraveno | 16. 11. 2021 9:42:51 |
Autor | Tayeb Basta | |
---|---|---|
Typ | Článek v časopise | |
Datum | 2020-12-10 | |
Abstrakt | In this work, we introduce a versatile ingredient that is available on our kitchen shelves. It is called sodium bicarbonate and commonly known as baking soda. We found out that this substance is cable of balancing the pH level in the human body. Scientists emphasize that acidosis in the blood is reflected by a drop of pH level to less than 7.35. On top of that, they asserted that the existence of acidosis in the blood simplifies the entry of viruses to host cells and then thrive and replicate inside their hosts. And based on these facts, we reach a conclusion stating that sodium bicarbonate is capable of defeating covid-19. | |
URL | https://www.researchgate.net/publication/346929536_Sodium_Bicarbonate_defeats_Coronavirus | |
Publikace | Researchgate | |
Katalog knihovny | ResearchGate | |
Select in Zotero | zotero://select/library/items/IUDTXLB6 | |
Datum přidání | 12. 4. 2021 9:17:18 | |
Upraveno | 4. 5. 2021 15:34:31 |
Autor | Rajpreet Kaur | |
---|---|---|
Autor | Anita Gupta | |
Typ | Článek v časopise | |
Datum | 2020-12-09 | |
Abstrakt | COVID-19, a pandemic, threatens the entire world by its multiplicative deadly behavior. Virus has caged the world’s people into their homes / isolation as their protective gear. Transmission rate, Case Fatality Rate, and Asymptomatic Transmission parameters determine the savage of COVID-19. More than a couple of months, mankind is staying locked in one’s home, which has never happened in the lifetime of any other generation. World Health Organization highlights the importance of hand Hygiene (Handwashing) by Soap/surfactant for 20 seconds and the use of alcohol-based Sanitizers as a part of personal health care. Soap functions as a weapon for sterilization of any viruses present on the surface. The lipid envelope of virusesis more susceptible to heat, desiccation or action of surfactants. Single tailed Quaternary ammonium compound surfactant is most commonly used as disinfectant or cleaning agent. Similarly, Gemini surfactants can also play a vital role as disinfectant / sanitizerin future. It is suggested that Gemini surfactants have imperative function in Air sanitation. Also, there is a valid comparison of Coronavirus with other SARS diseases. The deadly nature of virus can be understood by knowing its mechanism of action and mode of transmission. Once a chain of human to human transmission is initiated then it is very tricky to rupture the chain. | |
Přístup | 15. 12. 2020 12:01:31 | |
Publikace | International Journal of Engineering Technologies and Management Research | |
Jazyk | en | |
Ročník | 7 | |
Číslo | 12 | |
Rozsah | 1-16 | |
Práva | Copyright (c) 2020 Rajpreet Kaur, Anita Gupta | |
Extra | Number: 12 | |
ISSN | 2454-1907 | |
Katalog knihovny | www.granthaalayahpublication.org | |
DOI | 10.29121/ijetmr.v7.i12.2020.807 | |
Select in Zotero | zotero://select/library/items/DXISNP5G | |
Datum přidání | 15. 12. 2020 12:01:31 | |
Upraveno | 5. 3. 2021 10:33:12 |
Autor | Fatemeh Saadatpour | |
---|---|---|
Autor | Fatemeh Mohammadipanah | |
Typ | Článek v časopise | |
Datum | 2020-12-02 | |
Abstrakt | The transmission control of the newly emerged severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is the most effective strategy by the absence of its specified vaccine or drug. Although the aerosol mediated transmission of SARS‐CoV‐2 has been confirmed, the physicochemical treatment of the biotic and abiotic objects is still the most promising approach in its infection control. The front line of the most effective disinfecting compounds on SARS‐CoV‐2 implies to be sodium hypochlorite, ethanol, hydrogen peroxide, quaternary ammonium compounds, and phenolic compounds, respectively. However, widely used compounds of alkyldimethylbenzylammonium chloride (benzalkonium chloride) biguanides (chlorhexidine) have not shown the multitude load reduction in less than 10 minutes. The susceptibility of SARS‐CoV‐2 to physical treatment follows the pattern of heat, acidity, and UV radiation. Rather all of the mentioned physical or chemical treatments, target the envelope proteins of the coronavirus mainly by impairing its entry to host cells. The anti‐SARS‐CoV‐2 activity of combinatorial physicochemical treatments or evaluation of new chemical entities or physical treatments such as microwave irradiation still needs to be explored. Therefore, the development of a reliable decontamination protocol for SARS‐CoV‐2 demands revealing its stability pattern study vs a spectrum of single and combinatorial physicochemical parameters. | |
Publikace | Health Science Reports | |
Jazyk | English | |
Ročník | 3 | |
Číslo | 4 | |
Archiv | Coronavirus Research Database | |
Místo v archivu | 2466347748 | |
Extra | Place: Hoboken Publisher: John Wiley & Sons, Inc. | |
DOI | 10.1002/hsr2.213 | |
Select in Zotero | zotero://select/library/items/39N4PQ43 | |
Datum přidání | 14. 12. 2020 14:13:24 | |
Upraveno | 5. 3. 2021 10:33:17 |
Copyright - © 2020. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright - © 2020. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Název - Environmental Protection Agency--EPA
Poslední aktualizace - 2020-12-03
Poslední aktualizace - 2020-12-14
SubjectsTermNotLitGenreText - Middle East
Autor | Kaisen Lin | |
---|---|---|
Autor | Chase R Schulte | |
Autor | Linsey C Marr | |
Typ | Článek v časopise | |
Datum | 2020-12 | |
Abstrakt | The survival of viruses in droplets is known to depend on droplets’ chemical composition, which may vary in respiratory fluid between individuals and over the course of disease. This relationship is also important for understanding the persistence of viruses in droplets generated from wastewater, freshwater, and seawater. We investigated the effects of salt (0, 1, and 35 g/L), protein (0, 100, and 1000 μg/mL), surfactant (0, 1, and 10 μg/mL), and droplet pH (4.0, 7.0, and 10.0) on the viability of viruses in 1-μL droplets pipetted onto polystyrene surfaces and exposed to 20%, 50%, and 80% relative humidity (RH) using a culture-based approach. Results showed that viability of MS2, a non-enveloped virus, was generally higher than that of Φ6, an enveloped virus, in droplets after 1 hour. The chemical composition of droplets greatly influenced virus viability. Specifically, the survival of MS2 was similar in droplets at different pH values, but the viability of Φ6 was significantly reduced in acidic and basic droplets compared to neutral ones. The presence of bovine serum albumin protected both MS2 and Φ6 from inactivation in droplets. The effects of sodium chloride and the surfactant sodium dodecyl sulfate varied by virus type and RH. Meanwhile, RH affected the viability of viruses as shown previously: viability was lowest at intermediate to high RH. The results demonstrate that the viability of viruses is determined by the chemical composition of carrier droplets, especially pH and protein content, and environmental factors. These findings emphasize the importance of understanding the chemical composition of carrier droplets in order to predict the persistence of viruses contained in them. | |
Publikace | PLoS One | |
Jazyk | English | |
Ročník | 15 | |
Číslo | 12 | |
Archiv | Coronavirus Research Database; ProQuest Central | |
Místo v archivu | 2468200634 | |
Extra | Place: San Francisco Publisher: Public Library of Science | |
DOI | 10.1371/journal.pone.0243505 | |
Select in Zotero | zotero://select/library/items/AK2UD5SF | |
Datum přidání | 14. 12. 2020 15:40:55 | |
Upraveno | 5. 3. 2021 10:33:23 |
Copyright - © 2020 Lin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Název - Virginia Polytechnic Institute & State University
Poslední aktualizace - 2020-12-09
SubjectsTermNotLitGenreText - United States--US; Virginia
Autor | Tayeb Basta | |
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Typ | Článek v časopise | |
Datum | 2020-12 | |
Abstrakt | Scientists stress that the human body must keep its pH within the range of 7.35-7.45 to survive and function properly. Acidosis in the blood is reflected by a drop of pH level to less than 7.35. On top of that, they assert that the existence of acidosis in the blood simplifies the entry of viruses to host cells and then thrive and replicate. Based on these facts, we introduce a new strategic approach to fight against coronavirus. We suggest depleting the virus resources instead of destroying it through drugs or improving the immune system through vaccination. We introduce a versatile ingredient that is available on our kitchen shelves. It is called sodium bicarbonate and commonly known as baking soda. We found out that this substance can balance the pH level in the human body and consequently is capable of defeating covid-19. | |
URL | https://www.researchgate.net/profile/Tayeb-Basta/publication/346929536_Sodium_Bicarbonate_defeats_Coronavirus/links/606c1245458515614d3a55fc/Sodium-Bicarbonate-defeats-Coronavirus.pdf | |
Publikace | ResearchGate | |
Jazyk | en | |
Rozsah | 6 | |
Katalog knihovny | ResearchGate | |
Select in Zotero | zotero://select/library/items/LSPXBQ8M | |
Datum přidání | 3. 5. 2021 9:43:21 | |
Upraveno | 4. 5. 2021 15:34:30 |
Autor | Rodolfo A. Salido | |
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Autor | Sydney C. Morgan | |
Autor | Maria I. Rojas | |
Autor | Celestine G. Magallanes | |
Autor | Clarisse Marotz | |
Autor | Peter DeHoff | |
Autor | Pedro Belda-Ferre | |
Autor | Stefan Aigner | |
Autor | Deborah M. Kado | |
Autor | Gene W. Yeo | |
Autor | Jack A. Gilbert | |
Autor | Louise Laurent | |
Autor | Forest Rohwer | |
Autor | Rob Knight | |
Typ | Článek v časopise | |
Datum | 2020-11-23 | |
Abstrakt | Although SARS-CoV-2 is primarily transmitted by respiratory droplets and aerosols, transmission by fomites remains plausible. During Halloween, a major event for children in numerous countries, SARS-CoV-2 transmission risk via candy fomites worries many parents. To address this concern, we enrolled 10 recently diagnosed asymptomatic or mildly/moderately symptomatic COVID-19 patients to handle typical Halloween candy (pieces individually wrapped) under three conditions: normal handling with unwashed hands, deliberate coughing and extensive touching, and normal handling following handwashing. We then used a factorial design to subject the candies to two posthandling treatments: no washing (untreated) and household dishwashing detergent. We measured SARS-CoV-2 load by reverse transcriptase quantitative PCR (RT-qPCR) and loop-mediated isothermal amplification (LAMP). From the candies not washed posthandling, we detected SARS-CoV-2 on 60% of candies that were deliberately coughed on, 60% of candies normally handled with unwashed hands, but only 10% of candies handled after hand washing. We found that treating candy with dishwashing detergent reduced SARS-CoV-2 load by 62.1% in comparison to untreated candy. Taken together, these results suggest that although the risk of transmission of SARS-CoV-2 by fomites is low even from known COVID-19 patients, viral RNA load can be reduced to near zero by the combination of handwashing by the infected patient and ≥1 min detergent treatment after collection. We also found that the inexpensive and fast LAMP protocol was more than 80% concordant with RT-qPCR. IMPORTANCE The COVID-19 pandemic is leading to important tradeoffs between risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission and mental health due to deprivation from normal activities, with these impacts being especially profound in children. Due to the ongoing pandemic, Halloween activities will be curtailed as a result of the concern that candy from strangers might act as fomites. Here, we demonstrate that these risks can be mitigated by ensuring that, prior to handling candy, the candy giver washes their hands and, after receipt, by washing candy with household dishwashing detergent. Even in the most extreme case, with candy deliberately coughed on by known COVID-19 patients, viral load was reduced dramatically after washing with household detergent. We conclude that with reasonable precautions, even if followed only by either the candy giver or the candy recipient, the risk of viral transmission by this route is very low. | |
Přístup | 23. 11. 2020 11:05:51 | |
Publikace | mSystems | |
Jazyk | en | |
Ročník | 5 | |
Číslo | 6 | |
Práva | Copyright © 2020 Salido et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. | |
Extra | Publisher: American Society for Microbiology Journals Section: Observation PMID: 33127739 | |
ISSN | 2379-5077 | |
Katalog knihovny | msystems.asm.org | |
DOI | 10.1128/mSystems.01074-20 | |
Select in Zotero | zotero://select/library/items/ZY5P5Q4I | |
Datum přidání | 23. 11. 2020 11:05:51 | |
Upraveno | 5. 3. 2021 10:33:27 |
Autor | L. Coiffard | |
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Autor | C. Couteau | |
Typ | Článek v časopise | |
Datum | 2020-11-13 | |
Abstrakt | Soap has been used by humankind since ancient times and was probably already known to the Sumerians. It is a fatty acid salt obtained from the reaction of a strong base with a fatty substance of animal (tallow) or plant origin (oil). This reaction is called saponification. Syndets, on the other hand, are much more recent and have been in use for about a century. In the case of liquid syndets, they are mainly alkyl sulphates and their derivatives alkyl ether sulphates while isethionates and sarcosinates are more commonly found in solid syndets. Synthetic soaps and detergents are surfactants and, as such, they have detergent properties. The way soap works accounts for its antimicrobial properties. Thanks to its amphiphilic structure, it is able to interact with the lipid membranes of microorganisms (viruses, bacteria, etc.) and inactivate them. In this coronavirus pandemic period, health authorities worldwide recommend hand washing with soap and water. We therefore wanted to provide a summary of the chemical characteristics and applications of soaps, on the one hand, and synthetic detergents, on the other. Soap is not the only product used for hand hygiene and, given the current situation, alternatives are complex and varied. | |
Přístup | 23. 11. 2020 10:42:21 | |
Publikace | European Review for Medical and Pharmacological Sciences | |
Krátký název | Soap and syndets | |
Jazyk | en | |
Ročník | 24 | |
Číslo | 21 | |
Rozsah | 11432-11439 | |
Extra | WOS:000591376000065 | |
Katalog knihovny | Web of Science | |
DOI | 10.26355/eurrev_202011_23637 | |
Select in Zotero | zotero://select/library/items/2YRP3YY9 | |
Datum přidání | 23. 11. 2020 10:42:21 | |
Upraveno | 7. 7. 2021 15:35:30 |
Autor | Hideyuki Takano | |
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Typ | Článek v časopise | |
Datum | 2020-11 | |
Abstrakt | Pulmonary surfactant is considered to be one of the soaps. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the other enveloped viruses become very weak against surfactant. The SARS virus binds to angiotensin-converting enzyme (ACE2) receptor and causes pneumonia. In the lung, the ACE2 receptor sits on the top of lung cells known as alveolar epithelial type II (AE2) cells. These cells play an important role in producing surfactant. Pulmonary surfactant is believed to regulate the alveolar surface tension in mammalian lungs. To our knowledge, AE2 cells are believed to act as immunoregulatory cells; however, pulmonary surfactant itself has not been believed to act as a defender against the enveloped viruses. This study hypothesises that pulmonary surfactant may be a strong defender of enveloped viruses. Therefore, old coronaviruses merely cause pneumonia. On the contrary, new SARS-CoV-2 can suppress the production of surfactant that binds to the ACE2 of AE2 cells. The coronavirus can survive in the lung tissue because of the exhaustion of pulmonary surfactant. | |
Publikace | Medical Hypotheses | |
Ročník | 144 | |
Rozsah | 110020 | |
Extra | WOS:000593985600014 | |
Katalog knihovny | Web of Science | |
DOI | 10.1016/j.mehy.2020.110020 | |
Select in Zotero | zotero://select/library/items/EELKLRAN | |
Datum přidání | 7. 7. 2021 14:40:58 | |
Upraveno | 7. 7. 2021 15:36:52 |
Autor | Reza Jahromi | |
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Autor | Vahid Mogharab | |
Autor | Hossein Jahromi | |
Autor | Arezoo Avazpour | |
Typ | Článek v časopise | |
Datum | 2020-11 | |
Abstrakt | Our surrounding environment, especially often-touched contaminated surfaces, plays an important role in the transmission of pathogens in society. The shortage of effective sanitizing fluids, however, became a global challenge quickly after the coronavirus disease-19 (COVID-19) outbreak in December 2019. In this study, we present the effect of surfactants on coronavirus (SARS-CoV-2) viricidal efficiency in sanitizing fluids. Sodium dodecylbenzenesulfonate (SDBS), sodium laureth sulfate (SLS), and two commercial dish soap and liquid hand soap were studied with the goal of evaporation rate reduction in sanitizing liquids to maximize surface contact time. Twelve fluids with different recipes composed of ethanol, isopropanol, SDBS, SLS, glycerin, and water of standardized hardness (WSH) were tested for their evaporation time and viricidal efficiency. Evaporation time increased by 17-63% when surfactant agents were added to the liquid. In addition, surfactant incorporation enhanced the viricidal efficiency between 15 and 27% according to the 4-field test in the EN 16615:2015 European Standard method. Most importantly, however, we found that surfactant addition provides a synergistic effect with alcohols to inactivate the SARS-CoV-2 virus. This study provides a simple, yet effective solution to improve the virucidal efficiency of commonly used sanitizers. | |
Publikace | Food and Chemical Toxicology | |
Ročník | 145 | |
Rozsah | 111702 | |
Extra | WOS:000580355300039 | |
Katalog knihovny | Web of Science | |
DOI | 10.1016/j.fct.2020.111702 | |
Select in Zotero | zotero://select/library/items/CVHHMPBY | |
Datum přidání | 7. 7. 2021 14:40:58 | |
Upraveno | 7. 7. 2021 15:37:17 |
Autor | Stephen R. Welch | |
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Autor | Katherine A. Davies | |
Autor | Hubert Buczkowski | |
Autor | Nipunadi Hettiarachchi | |
Autor | Nicole Green | |
Autor | Ulrike Arnold | |
Autor | Matthew Jones | |
Autor | Matthew J. Hannah | |
Autor | Reah Evans | |
Autor | Christopher Burton | |
Autor | Jane E. Burton | |
Autor | Malcolm Guiver | |
Autor | Patricia A. Cane | |
Autor | Neil Woodford | |
Autor | Christine B. Bruce | |
Autor | Allen D. G. Roberts | |
Autor | Marian J. Killip | |
Typ | Článek v časopise | |
Datum | 2020-11 | |
Abstrakt | The COVID-19 pandemic has necessitated a multifaceted rapid response by the scientific community, bringing researchers, health officials, and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with the etiological agent, the novel human coronavirus SARS-CoV-2. Work with infectious SARS-CoV-2 is currently restricted to high-containment laboratories, but material can be handled at a lower containment level after inactivation. Given the wide array of inactivation reagents that are being used in laboratories during this pandemic, it is vital that their effectiveness is thoroughly investigated. Here, we evaluated a total of 23 commercial reagents designed for clinical sample transportation, nucleic acid extraction, and virus inactivation for their ability to inactivate SARS-CoV-2, as well as seven other common chemicals, including detergents and fixatives. As part of this study, we have also tested five filtration matrices for their effectiveness at removing the cytotoxic elements of each reagent, permitting accurate determination of levels of infectious virus remaining following treatment. In addition to providing critical data informing inactivation methods and risk assessments for diagnostic and research laboratories working with SARS-CoV-2, these data provide a framework for other laboratories to validate their inactivation processes and to guide similar studies for other pathogens. | |
Publikace | Journal of Clinical Microbiology | |
Ročník | 58 | |
Číslo | 11 | |
Rozsah | e01713-20 | |
Extra | WOS:000582218400031 | |
Katalog knihovny | Web of Science | |
DOI | 10.1128/JCM.01713-20 | |
Select in Zotero | zotero://select/library/items/9VKNHAC9 | |
Datum přidání | 7. 7. 2021 14:40:58 | |
Upraveno | 7. 7. 2021 15:36:40 |
Autor | Junmin Zhang | |
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Autor | Susan L. Morris‐Natschke | |
Autor | Di Ma | |
Autor | Xiao-Fei Shang | |
Autor | Chen-Jie Yang | |
Autor | Ying-Qian Liu | |
Autor | Kuo-Hsiung Lee | |
Typ | Článek v časopise | |
Datum | 2020-10-31 | |
Abstrakt | Indolizidine alkaloids are chemical constituents isolated from various marine and terrestrial plants and animals, including but not limited to trees, fungi, ants, and frogs, with a myriad of important biological activities. In this review, we discuss the biological activity and pharmacological effects of indolizidine alkaloids and offer new avenues toward the discovery of new and better drugs based on these naturally occurring compounds. | |
Přístup | 2. 11. 2020 10:24:20 | |
Publikace | Medicinal Research Reviews | |
Jazyk | en | |
Ročník | n/a | |
Číslo | n/a | |
Rozsah | 1-33 | |
Práva | © 2020 Wiley Periodicals LLC | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/med.21747 | |
ISSN | 1098-1128 | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1002/med.21747 | |
Select in Zotero | zotero://select/library/items/L5TY5A8U | |
Datum přidání | 2. 11. 2020 10:24:21 | |
Upraveno | 5. 3. 2021 10:34:10 |
Autor | Ibrahim M. Banat | |
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Autor | Quentin Carboué | |
Autor | Gerardo Saucedo-Castañeda | |
Autor | José de Jesús Cázares-Marinero | |
Typ | Článek v časopise | |
Datum | 2020-10-24 | |
Abstrakt | Surfactants are multipurpose products found in most sectors of contemporary industry. Their large-scale manufacturing has been mainly carried out using traditional chemical processes. Some of the chemical species involved in their production are considered hazardous and some industrial processes employing them categorised as “having potential negative impact on the environment”. Biological surfactants have therefore been generally accepted worldwide as suitable sustainable greener alternatives. Biosurfactants exhibit the same functionalities of synthetic analogues while having the ability to synergize with other molecules improving performances; this strengthens the possibility of reaching different markets via innovative formulations. Recently, their use was suggested to help combat Covid-19. In this review, an analysis of recent bibliography is presented with descriptions, statistics, classifications, applications, advantages, and challenges; evincing the reasons why biosurfactants can be considered as the chemical specialities of the future. Finally, the uses of the solid-state fermentation as a production technology for biosurfactants is presented. | |
Přístup | 2. 11. 2020 9:52:13 | |
Publikace | Bioresource Technology | |
Krátký název | Biosurfactants | |
Jazyk | en | |
Rozsah | 124222 | |
ISSN | 0960-8524 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Bioresource Technology | |
DOI | 10.1016/j.biortech.2020.124222 | |
Select in Zotero | zotero://select/library/items/AE9XXK6W | |
Datum přidání | 2. 11. 2020 9:52:13 | |
Upraveno | 5. 3. 2021 10:34:13 |
Autor | Gideon A. Gyebi | |
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Autor | Adegbenro P. Adegunloye | |
Autor | Ibrahim M. Ibrahim | |
Autor | Oludare M. Ogunyemi | |
Autor | Saheed O. Afolabi | |
Autor | Olalekan B. Ogunro | |
Typ | Článek v časopise | |
Datum | 2020-10-22 | |
Abstrakt | COVID-19 is a respiratory disease caused by SARS-CoV-2, an enveloped positive sense RNA virus. The SARS-CoV-2 spike glycoprotein, human angiotensin-converting enzyme 2 (ACE2) and human transmembrane protease serine 2 (TMPRSS2) are essential for the host cell-mediated viral entry. Targeting these proteins represent viable options to stop the first stage of infection and transmission. Hence, 97 alkaloids from African medicinal plants with reported antiviral activity were evaluated for this purpose via in silico studies. These alkaloids were docked for their interactions with SARS-CoV-2 spike glycoprotein, ACE2, and TMPRSS2. Top 20 alkaloids with highest binding affinities were further screened for their interactions with spike glycoprotein of SARS-CoV and MERS-CoV, and with ACE2-SARS-CoV-2 receptor-binding domain complex (ACE2-RBD). The energy profiling, molecular dynamics simulation (MDS), binding free energy base on Molecular Mechanics/Generalized Born Surface Area (MMGBSA), clustering of MDS trajectories, and virtual physicochemical and pharmacokinetic screening of the best docked alkaloids were performed. Results revealed that more than 15 alkaloids interacted better than the reference compounds. 10–Hydroxyusambarensine and Cryptospirolepine were docked in a similar binding pattern to the S1-specificy pocket of TMPRSS2 as camostat (reference inhibitor). The strong binding affinities, stability of the alkaloid-protein complexes and amino acid interactions displayed by cryptospirolepine, 10-hydroxyusambarensine, and cryptoquindoline with important binding hotspots of the proteins suggest these alkaloids have the potential of altering the capacity of SARS-CoV-2 membrane mediated host cell entry. Further in vitro and in vivo evaluation of these “drug-like” alkaloids as potential inhibitors of coronavirus cell entry is proposed. Communicated by Ramaswamy H. Sarma | |
Přístup | 2. 11. 2020 10:17:45 | |
Publikace | Journal of Biomolecular Structure and Dynamics | |
Krátký název | Prevention of SARS-CoV-2 cell entry | |
Ročník | 0 | |
Číslo | 0 | |
Rozsah | 1-25 | |
Extra | Publisher: Taylor & Francis _eprint: https://doi.org/10.1080/07391102.2020.1835726 PMID: 33089728 | |
ISSN | 0739-1102 | |
Katalog knihovny | Taylor and Francis+NEJM | |
DOI | 10.1080/07391102.2020.1835726 | |
Select in Zotero | zotero://select/library/items/FDGK9D78 | |
Datum přidání | 2. 11. 2020 10:17:45 | |
Upraveno | 5. 3. 2021 10:34:16 |
Autor | Mohana Devi Subramaniam | |
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Autor | Dhivya Venkatesan | |
Autor | Mahalaxmi Iyer | |
Autor | Sarathbabu Subbarayan | |
Autor | Vivekanandhan Govindasami | |
Autor | Ayan Roy | |
Autor | Arul Narayanasamy | |
Autor | Siva Kamalakannan | |
Autor | Abilash Valsala Gopalakrishnan | |
Autor | Raviminickam Thangarasu | |
Autor | Nachimuthu Senthil Kumar | |
Autor | Balachandar Vellingiri | |
Typ | Článek v časopise | |
Datum | 2020-09-30 | |
Abstrakt | Coronavirus Disease 2019 (COVID-19) has grown to be global public health emergency. The biosurfactants (BSs) are surface-active biomolecules with unique properties and wide applications. Several microbes synthesize secondary metabolites with surface-active properties which has wide range of anti-inflammatory and anti-viral role.. The monocytes and neutrophils are activated by bacteria which subsequently result in high secretion of pro-inflammatory cytokines (TNF-α, IL-6, IL-8, IL-12, Il-18 and IL-1β) and toll-like receptors-2 (TLR-2). Following the inflammatory response, BSs induce the production of cationic proteins, reactive oxygen species (ROS) and lysozyme, and thus can be utilized for therapeutic purposes. This review provides recent advances in the anti-inflammatory and antiviral activities of biosurfactants and discusses the potential use of these compounds against COVID-19, highlighting the need for in-vitro and in-vivo approaches to confirm this hypothesis. This suggestion is necessary because there are still no studies that have focused on the use of biosurfactants against COVID-19. | |
Přístup | 13. 10. 2020 13:45:28 | |
Publikace | Current Opinion in Environmental Science & Health | |
Krátký název | Biosurfactants and anti-inflammatory activity | |
Jazyk | en | |
ISSN | 2468-5844 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Current Opinion in Environmental Science & Health | |
DOI | 10.1016/j.coesh.2020.09.002 | |
Select in Zotero | zotero://select/library/items/Q86T7S7M | |
Datum přidání | 13. 10. 2020 13:45:28 | |
Upraveno | 5. 3. 2021 10:34:19 |
Autor | Lawrence Sheringham Borquaye | |
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Autor | Edward Ntim Gasu | |
Autor | Gilbert Boadu Ampomah | |
Autor | Lois Kwane Kyei | |
Autor | Margaret Amerley Amarh | |
Autor | Caleb Nketia Mensah | |
Autor | Daniel Nartey | |
Autor | Michael Commodore | |
Autor | Abigail Kusiwaa Adomako | |
Autor | Philipina Acheampong | |
Autor | Jehoshaphat Oppong Mensah | |
Autor | David Batsa Mormor | |
Autor | Caleb Impraim Aboagye | |
Typ | Článek v časopise | |
Datum | 2020-09-22 | |
Abstrakt | The ongoing global pandemic caused by the human coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has infected millions of people and claimed hundreds of thousands of lives. The absence of approved therapeutics to combat this disease threatens the health of all persons on earth and could cause catastrophic damage to society. New drugs are therefore urgently required to bring relief to people everywhere. In addition to repurposing existing drugs, natural products provide an interesting alternative due to their widespread use in all cultures of the world. In this study, alkaloids fromCryptolepis sanguinolentahave been investigated for their ability to inhibit two of the main proteins in SARS-CoV-2, the main protease and the RNA-dependent RNA polymerase, usingin silicomethods. Molecular docking was used to assess binding potential of the alkaloids to the viral proteins whereas molecular dynamics was used to evaluate stability of the binding event. The results of the study indicate that all 13 alkaloids bind strongly to the main protease and RNA-dependent RNA polymerase with binding energies ranging from -6.7 to -10.6 kcal/mol. In particular, cryptomisrine, cryptospirolepine, cryptoquindoline, and biscryptolepine exhibited very strong inhibitory potential towards both proteins. Results from the molecular dynamics study revealed that a stable protein-ligand complex is formed upon binding. Alkaloids fromCryptolepis sanguinolentatherefore represent a promising class of compounds that could serve as lead compounds in the search for a cure for the corona virus disease. | |
Publikace | Biomed Research International | |
Krátký název | Alkaloids from Cryptolepis sanguinolenta as Potential Inhibitors of SARS-CoV-2 Viral Proteins | |
Ročník | 2020 | |
Rozsah | 5324560 | |
Extra | WOS:000578289700002 | |
Katalog knihovny | Web of Science | |
DOI | 10.1155/2020/5324560 | |
Select in Zotero | zotero://select/library/items/D5PQQ2DW | |
Datum přidání | 7. 7. 2021 14:40:58 | |
Upraveno | 7. 7. 2021 15:37:55 |
Autor | Sayandip Mukherjee | |
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Autor | Carol K. Vincent | |
Autor | Harshinie W. Jayasekera | |
Autor | Ashish Shrikant Yekhe | |
Typ | Článek v časopise | |
Datum | 2020-09-17 | |
Abstrakt | Background Non-therapeutic interventions such as practicing good hand hygiene continue to be the mainstay of protection from SARS-CoV-2 and other emerging respiratory viruses. Methods We have evaluated a range of commercially available personal care products including soaps, handwash liquids and alcohol-based hand sanitizers for antiviral efficacy against a clinical isolate of SARS-CoV-2 using internationally accepted standardized protocols at user-relevant contact time-points and product dilutions. Results All the tested products resulted in 3 to 4 log reduction of SARS-CoV-2 titer. Conclusion Our data re-affirms recommendations by global public health authorities that proper hand hygiene can reduce SARS-CoV-2 viral load significantly which should likely limit spread of the contagion. | |
Přístup | 13. 10. 2020 13:51:50 | |
Publikace | Infection, Disease & Health | |
Jazyk | en | |
ISSN | 2468-0451 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Infection, Disease & Health | |
DOI | 10.1016/j.idh.2020.09.003 | |
Select in Zotero | zotero://select/library/items/487XS2ZI | |
Datum přidání | 13. 10. 2020 13:51:50 | |
Upraveno | 5. 3. 2021 10:34:23 |
Autor | Maurizio Manigrasso | |
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Autor | Carmela Protano | |
Autor | Ettore Guerriero | |
Autor | Matteo Vitali | |
Autor | Pasquale Avino | |
Typ | Článek v časopise | |
Datum | 2020-09-17 | |
Abstrakt | Ammonia is a common factor linking air in bat caves and air pollution in the proximity of agricultural fields treated with livestock farming sewage and slaughterhouses, where important clusters of COVID-19 have recently been reported all over the world. Such a commonality has a further connection with the known behavior of some viruses of the coronavirus family, such as the murine hepatitis virus, whose spike glycoprotein (S) can be triggered to a membrane-binding conformation at pH 8.0. Within the airborne route of virus transmission, with particular relevance for crowded and enclosed environments, these observations have prompted a hypothesis that may represent a contributing cause to interpret the geographical variability of the virus diffusion and the surging rise of COVID-19 cases in slaughterhouses all over the world. The hypothesis is that, in these environments, the SARS-CoV-2 S protein may find on a fraction of the airborne particles an alkaline pH, favorable to trigger the conformational changes, needed to induce the fusion of the viral envelope with the plasma membrane of the target cells. | |
Přístup | 13. 10. 2020 14:53:37 | |
Publikace | Atmosphere | |
Jazyk | en | |
Ročník | 11 | |
Číslo | 9 | |
Rozsah | 995 | |
Práva | http://creativecommons.org/licenses/by/3.0/ | |
Extra | Number: 9 Publisher: Multidisciplinary Digital Publishing Institute | |
Katalog knihovny | www.mdpi.com | |
DOI | 10.3390/atmos11090995 | |
Select in Zotero | zotero://select/library/items/J93DCJII | |
Datum přidání | 13. 10. 2020 14:53:37 | |
Upraveno | 5. 3. 2021 10:34:26 |
Autor | Nausheen Joondan | |
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Autor | Harsha Devi Angundhooa | |
Autor | Minu Gupta Bhowon | |
Autor | Prakashanand Caumul | |
Autor | Sabina Jhaumeer Laulloo | |
Typ | Článek v časopise | |
Datum | 2020-09-09 | |
Abstrakt | In this work, we report the application of coconut oil derived N-acyl prolinate surfactant as a potential ingredient in laundry detergent formulation using biological, physicochemical and molecular docking approach. The properties of the sodium cocyl prolinate surfactant 2 were compared with those of sodium cocoate surfactant 1, a sodium salt of fatty acids from coconut oil, as well as the conventional surfactant sodium dodecyl sulphate (SDS) which is commonly used in the detergent industry. Sodium cocyl prolinate surfactant 2 showed a lower foaming ability compared to SDS and was found to exert a better detergency at a much lower temperature (25°C) compared to surfactant 1 and SDS. The coconut oil based surfactants 1 and 2 displayed a better antibacterial activity against gram positive strains compared to SDS. In view of studying the effectiveness of the surfactants against the severe acute respiratory syndrome corona virus 2, SARS-CoV-2 (COVID-19) which might remain on the surface of contaminated clothes, molecular docking of surfactants 1 and 2 with the spike protein of SARS-CoV-2 was carried out. Surfactant 2 showed an optimum interaction with the amino acid residues of the spike protein which is responsible for the binding of the virus with the host receptors. Molecular docking with savinase, an enzyme used in laundry formulation showed that sodium cocyl prolinate surfactant 2 and SDS displayed comparable interactions with the enzyme. Overall, this study has shown that sodium cocyl prolinate surfactant 2 can be a potential candidate in laundry detergent formulation for machine washing due to its relatively low foaming ability and good detergency properties at a much lower temperature (25°C), making it more energy-efficient. Surfactant 2 was also found to be a promising antimicrobial agent in laundry detergent due to its moderate antibacterial activity and its interaction with the spike protein of SARS-CoV-2, which can help to reduce the spread of any epidemic or pandemic diseases. | |
Přístup | 13. 10. 2020 15:07:54 | |
Publikace | Tenside Surfactants Detergents | |
Ročník | 57 | |
Číslo | 5 | |
Rozsah | 361-374 | |
Extra | Publisher: Hanser Verlag | |
ISSN | 0932-3414 | |
Katalog knihovny | hanser-elibrary.com (Atypon) | |
Zkrácený název časopisu | TSD | |
DOI | 10.3139/113.110705 | |
Select in Zotero | zotero://select/library/items/SAV26UBH | |
Datum přidání | 13. 10. 2020 15:07:54 | |
Upraveno | 5. 3. 2021 10:34:34 |
Autor | National Health Library & Knowledge Service (NHLKS) | |
---|---|---|
Autor | Helen Clark | |
Autor | Gethin White | |
Autor | Siobhan McCarthy | |
Autor | Brendan Leen | |
Typ | Článek v časopise | |
Datum | 2020-08-20 | |
Abstrakt | ECDC notes that alcohol-based disinfectants have been shown to significantly reduce infectivity of enveloped viruses such as SARS-CoV-2 in concentrations of 70%-80% with one-minute exposure time2. CDC recommends that unless hands are visibly soiled, an alcohol-based hand rub is preferred over soap and water in most clinical scenarios due to evidence of better compliance compared to soap and water3. Hand rubs are generally less irritating to hands and are effective in the absence of a sink. CDC does not have a recommended alternative to hand rub products with greater than 60% ethanol or 70% isopropanol as active ingredients. Benzalkonium chloride along with both ethanol and isopropanol is deemed eligible by FDA for use in the formulation of hand rubs for use in healthcare settings. However, available evidence indicates that benzalkonium chloride has less reliable activity against certain bacteria and viruses than either of the alcohols. BMJ Best practice refers to CDC and WHO guidance, and cites the need to wash hands often with soap and water for at least 20 seconds, or with an alcohol-based hand sanitiser that contains at least 60% alcohol4. Berardi et al5 note that disinfectant effectiveness in alcohol-based hand rubs depends on: type of alcohol; concentrations; quantity applied on hands; and time of exposure. Isopropanol, ethanol, n-propanol or combinations of these alcohols are most commonly used in hand rubs. As distinct from other antiseptics, these alcohols do not have the potential for acquired bacterial resistance and none are effective against bacterial spores. When used at the same concentration, ethanol seems to have a lower bactericidal activity than propanols. However, ethanol has superior viricidal activity than propanols against non-enveloped viruses. Also, skin tolerance is better with ethanol compared to n-propanol or isopropanol. Thus, ethanol is often the alcohol of choice in alcohol-based hand rub preparations. Ethanol concentrations of 60% to 95% (v/v) are deemed safe and effective for disinfection by the United States Food and Drug Administration, CDC and the WHO, including for use against SARS-CoV-2. The choice for formulation rather than the concentration of alcohol is also an important consideration. The authors conclude that despite all of the products on the market ethanol content in alcohol-based hand rubs should be between 60% and 95%5. Kratzel et al found that SARS-CoV-2 was efficiently inactivated by WHO-recommended formulations, supporting their use in healthcare systems and viral outbreaks. The residual effect of hand sanitisers is discussed by Wilson et al, concluding that residual antinoroviral hand sanitizers may reduce infection risks for up to 4 hours. Appelgrein et al studied ozonated water compared with propanol-based hand rubs and found ozone to be inferior to propan-1-ol 60% hand rub for hand asepsis. However, Breideblik et al recommend ozonised water as an alternative that is viable especially for people with skin problems which alcohol-based products may adversely affect. | |
Přístup | 10. 9. 2020 11:09:51 | |
URL | https://www.lenus.ie/handle/10147/628144 | |
Krátký název | What is the efficacy of 60% alcohol-based hand gel vs non-alcohol-based hand gel for both COVID-19 and non-COVID-19 scenarios? | |
Jazyk | en | |
Extra | Accepted: 2020-08-25T14:12:45Z Publisher: Health Service Executive | |
Katalog knihovny | www.lenus.ie | |
Select in Zotero | zotero://select/library/items/HGNRYLTE | |
Datum přidání | 10. 9. 2020 11:09:51 | |
Upraveno | 4. 5. 2021 15:34:30 |
Autor | Luiz Alberto Colnago | |
---|---|---|
Autor | Iara Maria Trevisol | |
Autor | Daiane Voss Rech | |
Autor | Lucimara Aparecida Forato | |
Autor | Cirlei Igreja do Nascimento Mitre | |
Autor | José Paulo Gagliardi Leite | |
Autor | Cintia Hiromi Okino | |
Typ | Článek v časopise | |
Datum | 2020-08-19 | |
Abstrakt | COVID-19 infection, caused by SARS-CoV-2, is inequitably distributed and more lethal among populations with lower socioeconomic status. Direct contact with contaminated surfaces has been one of the virus sources, as it remains infective up to days. Several disinfectants have been shown to inactivate SARS-CoV-2 but they rapidly evaporate, are flammable or toxic and may be scarce or inexistent for the vulnerable populations. Therefore, we are proposing a simple, easy to prepare, low-cost and efficient antiviral films, made with wide available dishwasher detergent, which can be spread in hands and inanimate surfaces and maintains virucidal activity for longer periods than the current sanitizers. Avian coronavirus (ACoV) was used as model of challenge to test the antivirus efficacy of proposed films. Polystyrene microplates were covered with a thin layer of detergent formula. After drying, the films were exposed to different virus doses for 10 minutes and virus infectivity were determined using embryonated chicken eggs and RNA virus quantification in allantoic fluids by RT-qPCR. The films showed to inactive the ACoV (ranging from 103.66 to 106.66 EID50), which is chemically and morphologically similar to SARSCoV-2 and may constitute an excellent alternative to minimize the spread of Covid-19. | |
Publikace | MDPI AG Preprints | |
Jazyk | English | |
Archiv | Coronavirus Research Database; ProQuest Central | |
Místo v archivu | 2435337758 | |
Extra | Place: Basel Publisher: MDPI AG | |
DOI | 10.20944/preprints202008.0410.v1 | |
Select in Zotero | zotero://select/library/items/ZFXMSKK7 | |
Datum přidání | 9. 9. 2020 15:23:11 | |
Upraveno | 5. 3. 2021 10:34:44 |
Copyright - © 2020. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Poslední aktualizace - 2020-08-20
Autor | Edward I. Patterson | |
---|---|---|
Autor | Tessa Prince | |
Autor | Enyia R. Anderson | |
Autor | Aitor Casas-Sanchez | |
Autor | Shirley L. Smith | |
Autor | Cintia Cansado-Utrilla | |
Autor | Tom Solomon | |
Autor | Michael J. Griffiths | |
Autor | Álvaro Acosta-Serrano | |
Autor | Lance Turtle | |
Autor | Grant L. Hughes | |
Typ | Článek v časopise | |
Datum | 2020-08-15 | |
Abstrakt | The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels. | |
Přístup | 9. 9. 2020 15:16:09 | |
Publikace | The Journal of Infectious Diseases | |
Jazyk | en | |
Katalog knihovny | academic.oup.com | |
Zkrácený název časopisu | J Infect Dis | |
DOI | 10.1093/infdis/jiaa507 | |
Select in Zotero | zotero://select/library/items/88T88ISG | |
Datum přidání | 9. 9. 2020 15:16:09 | |
Upraveno | 5. 3. 2021 10:34:48 |
Autor | Edris Joonaki | |
---|---|---|
Autor | Aliakbar Hassanpouryouzband | |
Autor | Caryn L. Heldt | |
Autor | Oluwatoyin Areo | |
Typ | Článek v časopise | |
Datum | 2020-08-06 | |
Abstrakt | The surface stability and resulting transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), specifically in indoor environments, have been identified as a potential pandemic challenge requiring investigation. This novel virus can be found on various surfaces in contaminated sites such as clinical places; however, the behavior and molecular interactions of the virus with respect to the surfaces are poorly understood. Regarding this, the virus adsorption onto solid surfaces can play a critical role in transmission and survival in various environments. In this article, we first give an overview of existing knowledge concerning viral spread, molecular structure of SARS-CoV-2, and the virus surface stability is presented. Then, we highlight potential drivers of the SARS-CoV-2 surface adsorption and stability in various environmental conditions. This theoretical analysis shows that different surface and environmental conditions including temperature, humidity, and pH are crucial considerations in building fundamental understanding of the virus transmission and thereby improving safety practices. | |
Přístup | 19. 8. 2020 14:31:16 | |
Publikace | Chem | |
Jazyk | en | |
ISSN | 2451-9294 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Chem | |
DOI | 10.1016/j.chempr.2020.08.001 | |
Select in Zotero | zotero://select/library/items/H9JYRW7E | |
Datum přidání | 19. 8. 2020 14:31:16 | |
Upraveno | 5. 3. 2021 10:34:52 |
Autor | Tongqing Zhou | |
---|---|---|
Autor | Yaroslav Tsybovsky | |
Autor | Adam S Olia | |
Autor | Jason Gorman | |
Autor | Micah A Rapp | |
Autor | Gabriele Cerutti | |
Autor | Gwo-Yu Chuang | |
Autor | Phinikoula S Katsamba | |
Autor | Alexandra Nazzari | |
Autor | Jared M Sampson | |
Autor | Arne Schon | |
Autor | Pengfei D Wang | |
Autor | Jude Bimela | |
Autor | Wei Shi | |
Autor | Teng I-Ting | |
Autor | Baoshan Zhang | |
Autor | Jeffrey C Boyington | |
Autor | Mallika Sastry | |
Autor | Tyler Stephens | |
Autor | Jonathan Stuckey | |
Autor | Shuishu Wang | |
Autor | Richard A Friesner | |
Autor | David D Ho | |
Autor | John R Mascola | |
Autor | Lawrence Shapiro | |
Autor | Peter D Kwong | |
Typ | Článek v časopise | |
Datum | 2020-07-31 | |
Abstrakt | The SARS-CoV-2 spike employs mobile receptor-binding domains (RBDs) to engage the ACE2 receptor and to facilitate virus entry. Antibodies can engage RBD but some, such as CR3022, fail to inhibit entry despite nanomolar spike affinity. Here we show the SARS-CoV-2 spike to have low unfolding enthalpy at serological pH and up to 10-times more unfolding enthalpy at endosomal pH, where we observe significantly reduced CR3022 affinity. Cryo-EM structures -at serological and endosomal pH- delineated spike recognition of up to three ACE2 molecules, revealing RBD to freely adopt the 'up' conformation. In the absence of ACE2, single-RBD-up conformations dominated at pH 5.5, resolving into a locked all-down conformation at lower pH. Notably, a pH-dependent refolding region (residues 824-858) at the spike-interdomain interface displayed dramatic structural rearrangements and mediated RBD positioning and spike shedding of antibodies like CR3022. An endosomal mechanism involving spike-conformational change can thus facilitate immune evasion from RBD-'up'-recognizing antibody. Competing Interest Statement The authors have declared no competing interest. | |
Publikace | BioRxiv | |
Jazyk | English | |
Archiv | Coronavirus Research Database; ProQuest Central | |
Místo v archivu | 2429343649 | |
Extra | Place: Cold Spring Harbor Publisher: Cold Spring Harbor Laboratory Press | |
DOI | 10.1101/2020.07.04.187989 | |
Select in Zotero | zotero://select/library/items/6F69BPMX | |
Datum přidání | 19. 8. 2020 14:14:05 | |
Upraveno | 5. 3. 2021 10:34:55 |
Copyright - © 2020. This article is published under https://creativecommons.org/publicdomain/zero/1.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Poslední aktualizace - 2020-08-01
Autor | Kwok-Hung Chan | |
---|---|---|
Autor | Siddharth Sridhar | |
Autor | Ricky Ruiqi Zhang | |
Autor | Hin Chu | |
Autor | Agnes Yim-Fong Fung | |
Autor | Gabriella Chan | |
Autor | Jasper Fuk-Woo Chan | |
Autor | Kelvin Kai-Wang To | |
Autor | Ivan Fan-Ngai Hung | |
Autor | Vincent Chi-Chung Cheng | |
Autor | Kwok-Yung Yuen | |
Typ | Článek v časopise | |
Datum | 2020-07-09 | |
Abstrakt | Background In late 2019, a novel human coronavirus, SARS-CoV-2, emerged in Wuhan, China. This virus has caused a global pandemic involving more than 200 countries. SARS-CoV-2 is highly adapted to humans and readily transmits from person-to-person. Aim The aim of this study was to investigate the infectivity of SARS-CoV-2 under various environmental factors, disinfectants and different pH conditions. The efficacy of a variety of laboratory virus inactivation methods and home disinfectants against SARS-CoV-2 were investigated. Methods The residual virus in dried form or in solution was titrated on Vero E6 cell line at day 0, 1, 3, 5, and 7 after incubation at different temperatures. The viability of virus was determined after treatment with different disinfectants and pH solutions at room temperature (20∼25oC). Findings SARS-CoV-2 was able to retain viability for 3-5 days in dried form or 7 days in solution at room temperature. SARS-CoV-2 could be detected under a wide range of pH conditions from pH4 to pH11 for several days and 1 to 2 days in stool at room temperature but lost 5 logs of infectivity. A variety of commonly used disinfectants and laboratory inactivation procedures were found to reduce viral viability effectively. Conclusion This study demonstrates the stability of SARS-CoV-2 on environmental surfaces and raises the possibility of faecal-oral transmission. Commonly used fixatives, nucleic acid extraction methods and heat inactivation were found to significantly reduce viral infectivity that could ensure hospital and laboratory safety during the COVID-19 pandemic. | |
Přístup | 28. 7. 2020 9:00:29 | |
Publikace | Journal of Hospital Infection | |
Jazyk | en | |
ISSN | 0195-6701 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Hospital Infection | |
DOI | 10.1016/j.jhin.2020.07.009 | |
Select in Zotero | zotero://select/library/items/M36S6YUU | |
Datum přidání | 27. 7. 2020 13:14:14 | |
Upraveno | 5. 3. 2021 10:34:58 |
Autor | K. Pramod | |
---|---|---|
Autor | Sabna Kotta | |
Autor | U. S. Jijith | |
Autor | A. Aravind | |
Autor | M. Abu Tahir | |
Autor | C. S. Manju | |
Autor | H. V. Gangadharappa | |
Typ | Článek v časopise | |
Datum | 2020-07-07 | |
Abstrakt | Hand hygiene by washing with soap and water is recommended for the prevention of COVID-19 spread. Soaps and detergents are explained to act by damaging viral spike glycoproteins (peplomers) or by washing out the virus through entrapment in the micelles. Technically, soaps come under a functional category of molecules known as surfactants. Surfactants are widely used in pharmaceutical formulations as excipients. We wonder why surfactants are still not tried for prophylaxis or therapy against COVID-19? That too when many of them have proven antiviral properties. Moreover, lung surfactants have already shown benefits in respiratory viral infections. Therefore, we postulate that surfactant-based prophylaxis and therapy would be promising. We believe that our hypothesis would stimulate debate or new research exploring the possibility of surfactant-based prophylaxis and therapy against COVID-19. The success of a surfactant-based technique would save the world from any such pandemic in the future too. | |
Přístup | 28. 7. 2020 9:04:38 | |
Publikace | Medical Hypotheses | |
Krátký název | Surfactant-based prophylaxis and therapy against COVID-19 | |
Jazyk | en | |
Ročník | 143 | |
Rozsah | 110081 | |
ISSN | 0306-9877 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Medical Hypotheses | |
DOI | 10.1016/j.mehy.2020.110081 | |
Select in Zotero | zotero://select/library/items/TNCIKEYA | |
Datum přidání | 28. 7. 2020 9:04:38 | |
Upraveno | 5. 3. 2021 10:35:03 |
Autor | M. A. Islam | |
---|---|---|
Typ | Článek v časopise | |
Datum | 2020-06-12 | |
Abstrakt | Present study has been designed to clarify the mechanism of inactivation of the RNA enveloped viruses in-vitro. For that, avian Coronavirus (Avian Infectious Bronchitis Virus, AIBV) and Bird flu virus (Low Pathogenic Avian Influenza Virus, LPAIV, H9N2) were selected for this study. Both the viruses were subjected to heat (25 to 70°C and pH (1.0 to 14.0) treatment for 1 to 120 min and 30 s to 5 min respectively and their infectivity was checked by measuring virus titre through hemagglutination (HA) test, chicken embryo inoculation and RT-iiPCR. The results clearly indicates that both the viruses required 55°C for 90 min, 60°C for 60 min, 65°C for 15 min and 70°C for 2 and 3 min respectively to inactivate. Results of pH sensitivity using various detergents and chemicals exposure indicates that the agents having moderate acidic pH (5.0 to 6.0), neutral to moderate alkaline pH (7.0 to 10.0) and extreme alkaline pH (13.0 to 14.0) could inactivate AIBV within 20 to 30 s and the H9N2 virus was inactivated in pH range <3.0 and 13.0 to 14.0 within 5 min. The 70% ethanol or isopropanol was found highly effective to inactivate both the viruses in a minute. More interestingly, juice of various citrus fruits also exhibited antiviral activities in-vitro. Results of the present study indicated that the enveloped RNA viruses of any families could easily be inactivated using very cheap and available chemicals and detergents. Chicken embryos propagation and molecular detection by RT-iiPCR also indicated that ultra-acidic and extreme alkaline pH and higher temperature treatment successfully inactivated both the viruses. Finding of the present study also indicated that any viruses of the family coronaviridae including SARS-CoV-2 and other respiratory viruses of human and animal can be inactivated easily by exposure at high temperature and extremely low and high pH treatment. | |
Přístup | 7. 7. 2020 10:31:53 | |
Publikace | International Research Journal of Medicine and Medical Sciences | |
Krátký název | Thermal and pH sensitivity of avian corona and influenza viruses | |
Ročník | 8 | |
Číslo | 2 | |
Rozsah | 42-56 | |
Katalog knihovny | www.netjournals.org | |
DOI | 10.30918/IRJMMS.82.20.028 | |
Select in Zotero | zotero://select/library/items/BR7QISDI | |
Datum přidání | 7. 7. 2020 10:31:53 | |
Upraveno | 5. 3. 2021 10:35:05 |
Autor | Reza Jahromi | |
---|---|---|
Autor | Vahid Mogharab | |
Autor | Hossein Jahromi | |
Autor | Arezoo Avazpour | |
Typ | Článek v časopise | |
Datum | 2020-06-01 | |
Abstrakt | Our surrounding environment, especially often-touched contaminated surfaces, plays an important role in the transmission of pathogens in society. The shortage of effective sanitizing fluids, however, became a global challenge quickly after the coronavirus disease-19 (COVID-19) outbreak in December 2019. In this study, we present the effect of surfactants on coronavirus (SARS-CoV-2) virucidal efficiency in sanitizing fluids. Sodium dodecylbenzenesulfonate (SDBS), sodium laureth sulfate (SLS), and two commercial dish soap and liquid hand soap were studied with the goal of evaporation rate reduction in sanitizing liquids to maximize surface contact time. Twelve fluids with different recipes composed of ethanol, isopropanol, SDBS, SLS, glycerin, and water of standardized hardness (WSH) were tested for their evaporation time and virucidal efficiency. Evaporation time increased by 17-63% when surfactant agents were added to the liquid. In addition, surfactant incorporation enhanced the virucidal efficiency between 15-27% according to the 4-field test in the EN 16615:2015 European Standard method. Most importantly, however, we found that surfactant addition provides a synergistic effect with alcohols to inactivate the SARS-CoV-2 virus. This study provides a simple, yet effective solution to improve the virucidal efficiency of commonly used sanitizers. | |
Přístup | 7. 7. 2020 10:39:39 | |
Publikace | bioRxiv | |
Jazyk | en | |
Rozsah | 2020.05.29.124107 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Section: New Results | |
Katalog knihovny | www.biorxiv.org | |
DOI | 10.1101/2020.05.29.124107 | |
Select in Zotero | zotero://select/library/items/9XMTM6FT | |
Datum přidání | 7. 7. 2020 10:39:39 | |
Upraveno | 5. 3. 2021 10:35:09 |
Autor | Qing-Xia Ma | |
---|---|---|
Autor | Shan Hu | |
Autor | Hong-Liang Zhang | |
Autor | Gui-Mei Li | |
Autor | Rui-Mei Yang | |
Autor | Ji-Ming Chen | |
Typ | Článek v časopise | |
Datum | 2020-05-31 | |
Abstrakt | The surge of patients in the pandemic of COVID‐19 caused by the novel coronavirus SARS‐CoV‐2 may overwhelm the medical systems of many countries. Mask‐wearing and handwashing can slow the spread of the virus, but currently, masks are in shortage in many countries, and timely handwashing is often impossible. In this study, the efficacy of three types of masks and instant hand wiping was evaluated using the avian influenza virus to mock the coronavirus. Virus quantification was performed using real‐time reverse transcription‐polymerase chain reaction. Previous studies on mask‐wearing were reviewed. The results showed that instant hand wiping using a wet towel soaked in water containing 1.00% soap powder, 0.05% active chlorine, or 0.25% active chlorine from sodium hypochlorite removed 98.36%, 96.62%, and 99.98% of the virus from hands, respectively. N95 masks, medical masks, and homemade masks made of four‐layer kitchen paper and one‐layer cloth could block 99.98%, 97.14%, and 95.15% of the virus in aerosols. Medical mask‐wearing which was supported by many studies was opposed by other studies possibly due to erroneous judgment. With these data, we propose the approach of mask‐wearing plus instant hand hygiene (MIH) to slow the exponential spread of the virus. This MIH approach has been supported by the experiences of seven countries in fighting against COVID‐19. Collectively, a simple approach to slow the exponential spread of SARS‐CoV‐2 was proposed with the support of experiments, literature review, and control experiences. | |
Přístup | 18. 5. 2020 13:25:08 | |
Publikace | Journal of Medical Virology; Hoboken | |
Jazyk | English | |
Práva | © 2020. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the associated terms available at https://novel-coronavirus.onlinelibrary.wiley.com/ | |
Extra | Place: Hoboken, United States, Hoboken Publisher: John Wiley & Sons, Inc. | |
ISSN | 1096-9071 | |
Katalog knihovny | ProQuest | |
DOI | 10.1002/jmv.25805 | |
Select in Zotero | zotero://select/library/items/63BM3GFW | |
Datum přidání | 18. 5. 2020 13:25:08 | |
Upraveno | 5. 3. 2021 10:35:12 |
Autor | Christin Scheller | |
---|---|---|
Autor | Finja Krebs | |
Autor | Robert Minkner | |
Autor | Isabel Astner | |
Autor | Maria Gil‐Moles | |
Autor | Hermann Wätzig | |
Typ | Článek v časopise | |
Datum | 2020-05-29 | |
Abstrakt | The material properties of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and its proteins are discussed. We review the viral structure, size, rigidity, lipophilicity, isoelectric point, buoyant density and centrifugation conditions, stability against pH, temperature, UV light, gamma radiation and susceptibility to various chemical agents, including solvents and detergents. Possible inactivation, downstream and formulation conditions are given, including suitable buffers and some first ideas for quality‐control methods. This information supports vaccine development and discussion with competent authorities during vaccine approval and is certainly related to drug‐targeting strategies and hygienics. Several instructive tables are given, including the pI and grand average of hydropathicity (GRAVY) of SARS‐CoV‐1 and ‐2 proteins in comparison. SARS‐CoV‐1 and SARS‐CoV‐2 are similar in many regards, so information can often be derived. Both are unusually stable, but sensitive at their lipophilic membranes. However, since seemingly small differences can have strong effects, e.g., on immunologically relevant epitope settings, unevaluated knowledge transfer from SARS‐CoV‐1 to SARS‐CoV‐2 cannot be advised. Published knowledge regarding downstream processes, formulations and quality assuring methods is, as yet, limited. However, standard approaches employed for other viruses and vaccines seem to be feasible, including virus inactivation, centrifugation conditions and the use of adjuvants. This article is protected by copyright. All rights reserved | |
Přístup | 7. 7. 2020 7:07:53 | |
Publikace | ELECTROPHORESIS | |
Jazyk | English | |
Práva | © 2020. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the associated terms available at https://novel-coronavirus.onlinelibrary.wiley.com/ | |
Extra | Place: Hoboken, United States, Hoboken Publisher: John Wiley & Sons, Inc. | |
ISSN | 1522-2683 | |
Katalog knihovny | ProQuest | |
DOI | 10.1002/elps.202000121 | |
Select in Zotero | zotero://select/library/items/5I2L6TTP | |
Datum přidání | 7. 7. 2020 7:07:54 | |
Upraveno | 5. 3. 2021 10:35:14 |
Autor | Jane Lee Jia Jing | |
---|---|---|
Autor | Thong Pei Yi | |
Autor | Rajendran J. C. Bose | |
Autor | Jason R. McCarthy | |
Autor | Nagendran Tharmalingam | |
Autor | Thiagarajan Madheswaran | |
Typ | Článek v časopise | |
Datum | 2020-05-11 | |
Abstrakt | Hand hygiene is of utmost importance as it may be contaminated easily from direct contact with airborne microorganism droplets from coughs and sneezes. Particularly in situations like pandemic outbreak, it is crucial to interrupt the transmission chain of the virus by the practice of proper hand sanitization. It can be achieved with contact isolation and strict infection control tool like maintaining good hand hygiene in hospital settings and in public. The success of the hand sanitization solely depends on the use of effective hand disinfecting agents formulated in various types and forms such as antimicrobial soaps, water-based or alcohol-based hand sanitizer, with the latter being widely used in hospital settings. To date, most of the effective hand sanitizer products are alcohol-based formulations containing 62%–95% of alcohol as it can denature the proteins of microbes and the ability to inactivate viruses. This systematic review correlated with the data available in Pubmed, and it will investigate the range of available hand sanitizers and their effectiveness as well as the formulation aspects, adverse effects, and recommendations to enhance the formulation efficiency and safety. Further, this article highlights the efficacy of alcohol-based hand sanitizer against the coronavirus. | |
Přístup | 10. 9. 2020 11:07:46 | |
Publikace | International Journal of Environmental Research and Public Health | |
Krátký název | Hand Sanitizers | |
Jazyk | en | |
Ročník | 17 | |
Číslo | 9 | |
Práva | http://creativecommons.org/licenses/by/3.0/ | |
Extra | Number: 9 Publisher: Multidisciplinary Digital Publishing Institute | |
Katalog knihovny | www.mdpi.com | |
DOI | 10.3390/ijerph17093326 | |
Select in Zotero | zotero://select/library/items/CUV9YUA4 | |
Datum přidání | 10. 9. 2020 11:07:46 | |
Upraveno | 5. 3. 2021 10:35:17 |
Autor | Narendra Kumar Chaudhary | |
---|---|---|
Autor | Nabina Chaudhary | |
Autor | Manis Dahal | |
Autor | Biswash Guragain | |
Autor | Sumie Rai | |
Autor | Rahul Chaudhary | |
Autor | K. M. Sachin | |
Autor | Reena Lamichhane-Khadka | |
Autor | Ajaya Bhattarai | |
Typ | Článek v časopise | |
Datum | 2020-05-07 | |
Abstrakt | Today, the entire globe is struggling to deal with the greatest pandemic of the century, COVID-19. With no clinically approved treatments available, we are left with no options other than following the preventive measures issued by the World Health Organization (WHO). Among many others, hand washing with soap and water has been emphasized the most because it is cost-effective and easily accessible to the general public. Various studies have reported that soaps offer unique chemical properties that can disinfect the virus as a whole. However, there is still ambiguity in the general public about whether soaps can really shield us from this highly contagious disease. In an attempt to help eliminate the ambiguity, we analyzed the mechanisms underlying the efficacy of soap and its prospect for preventing the spread of COVID-19. In this paper, we have provided an overview of the history and characteristics of SARS-CoV-2 (COVID-19), the detailed mechanisms of the deactivation of viruses by soaps, and the potential effectiveness of soap in eliminating coronaviruses including SARS-CoV-2. | |
Přístup | 27. 5. 2020 9:45:38 | |
URL | https://www.preprints.org/manuscript/202005.0060/v2 | |
Jazyk | en | |
Extra | Publisher: Preprints | |
Katalog knihovny | www.preprints.org | |
Select in Zotero | zotero://select/library/items/YCHQC2SW | |
Datum přidání | 27. 5. 2020 9:45:38 | |
Upraveno | 4. 5. 2021 15:34:29 |
Autor | Indranil Chakraborty | |
---|---|---|
Typ | Článek v časopise | |
Datum | 2020-05-07 | |
Abstrakt | The emergence of SARS-CoV-2 is the third introduction of a highly pathogenic coronavirus into the human population after the severe acute respiratory syndrome coronavirus (SARS-COV) and the Middle East respiratory syndrome coronavirus (MERS-COV). Even after taking thousands of human lives, there is no effective curative treatment till date. This virus infects human body by means of aerosol, droplets and contact. Mucous membrane mainly respiratory epithelium is the point of entry in case of SARS-COV2. Then it fuses with the pneumocytes and macrophages of respiratory epithelium by a complex protein-protein interaction. And this initial vital process of fusion needs acidic micro-environment. Here I propose a novel therapeutic approach that can prevent the entry of the virus into host cell and thus will curb down the infection rate and its severity significantly. Moderate alkaline solution of sodium bicarbonate will be used by a nebulizer, rotahaler or inhaler to the respiratory tract to make the airway surface fluid alkaline from acidic and this will hinder the entry of virus to host cells. | |
Přístup | 20. 8. 2020 12:24:56 | |
Publikace | Journal of Current Medical Research and Opinion | |
Jazyk | en | |
Ročník | 3 | |
Číslo | 05 | |
Rozsah | 465–467 | |
Práva | Copyright (c) 2020 Journal of Current Medical Research and Opinion | |
Extra | Number: 05 | |
ISSN | 2589-8779 | |
Katalog knihovny | cmro.in | |
Zkrácený název časopisu | 1 | |
DOI | 10.15520/jcmro.v3i05.289 | |
Select in Zotero | zotero://select/library/items/L67FZ3KC | |
Datum přidání | 20. 8. 2020 12:24:56 | |
Upraveno | 5. 3. 2021 10:35:22 |
Autor | Alex W H Chin | |
---|---|---|
Autor | Julie T S Chu | |
Autor | Mahen R A Perera | |
Autor | Kenrie P Y Hui | |
Autor | Hui-Ling Yen | |
Autor | Michael C W Chan | |
Autor | Malik Peiris | |
Autor | Leo L M Poon | |
Typ | Článek v časopise | |
Datum | 2020-05-01 | |
Abstrakt | We previously reported the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in different clinical samples.1 This virus can be detected on different surfaces in a contaminated site.2 Here, we report the stability of SARS-CoV-2 in different environmental conditions. | |
Přístup | 12. 4. 2021 9:47:47 | |
URL | https://www.sciencedirect.com/science/article/pii/S2666524720300033 | |
Publikace | The Lancet Microbe | |
Jazyk | en | |
Ročník | 1 | |
Číslo | 1 | |
Rozsah | e10 | |
ISSN | 2666-5247 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | The Lancet Microbe | |
DOI | 10.1016/S2666-5247(20)30003-3 | |
Select in Zotero | zotero://select/library/items/UZMKINE4 | |
Datum přidání | 12. 4. 2021 9:47:47 | |
Upraveno | 12. 4. 2021 9:49:54 |
Autor | Bhawani Singh Sonigara | |
---|---|---|
Autor | KumKum Sarangdevot | |
Autor | M S Ranawat | |
Typ | Článek v časopise | |
Datum | 2020-05 | |
Abstrakt | A virus is made up of nucleic acid genome (RNA or DNA), Protein, and outer fatty layer. All these three structure forms the complete structure of virus. These all are attached too weakly with each other. The attachment is non- covalent in nature and interacts with protein, RNA and lipids. Together these act together like a Velcro so it is very hard to break up the self-assembled viral particle. But the soap makes it easy to break this bond by dissolving the lipid layer and also weakening the other bonding within the virus molecule and thus the virus dies and falls apart. Soap contains amphiphiles, which are fat-like compounds and are similar to the lipids found in the membrane of virus. | |
URL | http://chemrj.org/download/vol-5-iss-3-2020/chemrj-2020-05-03-24-27.pdf | |
Publikace | Chemistry Research Journal | |
Jazyk | en | |
Ročník | 5 | |
Číslo | 3 | |
Rozsah | 24-27 | |
ISSN | 2455-8990 | |
Katalog knihovny | Zotero | |
Select in Zotero | zotero://select/library/items/IVJUMEF9 | |
Datum přidání | 7. 7. 2020 10:49:42 | |
Upraveno | 4. 5. 2021 15:34:31 |
Autor | Huajun Zhang | |
---|---|---|
Autor | Chen Peng | |
Autor | Bobo Liu | |
Autor | Jun Liu | |
Autor | Zhiming Yuan | |
Autor | Zhengli Shi | |
Typ | Článek v časopise | |
Datum | 2018-03-1 | |
Abstrakt | MICRO-CHEM PLUS Detergent Disinfectant Cleaner (MCP) is a commonly used disinfectant at biosafety level 4 (BSL-4) laboratories where research activities involving the most dangerous pathogens must be conducted. Using bat severe acute respiratory syndrome (SARS)–like coronavirus (CoV) WIV1 as a surrogate pathogen, we extensively evaluated the disinfection efficacy of 5% MCP in the first BSL-4 laboratory in China. WIV1 was completely inactivated in 1 minute of contact time by a 27-fold dilution of 5% MCP with a titer reduction of more than 7 lg 50% tissue culture infectious dose (TCID50)/mL, while a 243-fold dilution of 5% MCP showed very weak activity. For 81-fold dilution, WIV1 was killed by more than 6 lg TCID50/mL in 1 minute, but to get complete inactivation, the contact time should be at least 2 minutes. No loss of efficacy was detected for 5% MCP at a temperature of 0°C, ambient, or 37°C or in the solution of pH 3.2 to 12.2. No interference to disinfection efficacy was detected with up to 25% fetal bovine serum, 5% bovine serum albumin, or 8% cell debris. The decontamination of a positive pressure suit by chemical shower was tested with the filter pieces taped onto the head, chest, back, armpits, crotch, and boot soles, and no virus survived after a shower cycle of 4 minutes at 5% MCP plus 2 minutes of a soft water rinse. Keeping the ambient temperature for 79 days or in the tank for the chemical shower for 25 days, 5% MCP showed the same activity as when it was freshly prepared. Taken together, 5% MCP is a very efficient disinfectant for BSL-4 laboratories, and we recommend its application for other enveloped viruses. | |
Přístup | 26. 5. 2020 13:49:08 | |
Publikace | Applied Biosafety | |
Jazyk | en | |
Ročník | 23 | |
Číslo | 1 | |
Rozsah | 32-38 | |
Extra | Publisher: SAGE Publications Inc | |
ISSN | 1535-6760 | |
Katalog knihovny | SAGE Journals | |
Zkrácený název časopisu | Appl Biosaf. | |
DOI | 10.1177/1535676018758891 | |
Select in Zotero | zotero://select/library/items/QB6KGRXR | |
Datum přidání | 26. 5. 2020 13:49:08 | |
Upraveno | 5. 3. 2021 10:35:29 |
Autor | Chloe Geller | |
---|---|---|
Autor | Mihayl Varbanov | |
Autor | Raphaël E. Duval | |
Typ | Článek v časopise | |
Datum | 2012-11-12 | |
Abstrakt | The Coronaviridae family, an enveloped RNA virus family, and, more particularly, human coronaviruses (HCoV), were historically known to be responsible for a large portion of common colds and other upper respiratory tract infections. HCoV are now known to be involved in more serious respiratory diseases, i.e. bronchitis, bronchiolitis or pneumonia, especially in young children and neonates, elderly people and immunosuppressed patients. They have also been involved in nosocomial viral infections. In 2002-2003, the outbreak of severe acute respiratory syndrome (SARS), due to a newly discovered coronavirus, the SARS-associated coronavirus (SARS-CoV); led to a new awareness of the medical importance of the Coronaviridae family. This pathogen, responsible for an emerging disease in humans, with high risk of fatal outcome; underline the pressing need for new approaches to the management of the infection, and primarily to its prevention. Another interesting feature of coronaviruses is their potential environmental resistance, despite the accepted fragility of enveloped viruses. Indeed, several studies have described the ability of HCoVs (i.e. HCoV 229E, HCoV OC43 (also known as betacoronavirus 1), NL63, HKU1 or SARS-CoV) to survive in different environmental conditions (e.g. temperature and humidity), on different supports found in hospital settings such as aluminum, sterile sponges or latex surgical gloves or in biological fluids. Finally, taking into account the persisting lack of specific antiviral treatments (there is, in fact, no specific treatment available to fight coronaviruses infections), the Coronaviridae specificities (i.e. pathogenicity, potential environmental resistance) make them a challenging model for the development of efficient means of prevention, as an adapted antisepsis-disinfection, to prevent the environmental spread of such infective agents. This review will summarize current knowledge on the capacity of human coronaviruses to survive in the environment and the efficacy of well-known antiseptic-disinfectants against them, with particular focus on the development of new methodologies to evaluate the activity of new antiseptic-disinfectants on viruses. | |
Přístup | 25. 5. 2020 11:56:17 | |
Publikace | Viruses; Basel | |
Krátký název | Human Coronaviruses | |
Jazyk | English | |
Ročník | 4 | |
Číslo | 11 | |
Rozsah | 3044-3068 | |
Práva | Copyright MDPI AG 2012 | |
Extra | Num Pages: 3044-3068 Place: Basel, Switzerland, Basel Publisher: MDPI AG | |
Katalog knihovny | ProQuest | |
DOI | 10.3390/v4113044 | |
Select in Zotero | zotero://select/library/items/EFTJFVE4 | |
Datum přidání | 25. 5. 2020 11:56:17 | |
Upraveno | 5. 3. 2021 10:35:32 |
Autor | Christine Dellanno | |
---|---|---|
Autor | Quinn Vega | |
Autor | Diane Boesenberg | |
Typ | Článek v časopise | |
Datum | 2009-10-01 | |
Abstrakt | Background The 2003 outbreak of severe acute respiratory syndrome (SARS) infected over 8000 people and killed 774. Transmission of SARS occurred through direct and indirect contact and large droplet nuclei. The World Health Organization recommended the use of household disinfectants, which have not been previously tested against SARS coronavirus (SARS-CoV), to disinfect potentially contaminated environmental surfaces. There is a need for a surrogate test system given the limited availability of the SARS-CoV for testing and biosafety requirements necessary to safely handle it. In this study, the antiviral activity of standard household products was assayed against murine hepatitis virus (MHV), as a potential surrogate for SARS-CoV. Methods A surface test method, which involves drying an amount of virus on a surface and then applying the product for a specific contact time, was used to determine the virucidal activity. The virus titers and log reductions were determined by the Reed and Muench tissue culture infective dose (TCID)50 end point method. Results When tested as directed, common household disinfectants or antiseptics, containing either 0.050% of triclosan, 0.12% of PCMX, 0.21% of sodium hypochlorite, 0.23% of pine oil, or 0.10% of a quaternary compound with 79% of ethanol, demonstrated a 3-log reduction or better against MHV without any virus recovered in a 30-second contact time. Conclusion Common household disinfectants and antiseptics were effective at inactivating MHV, a possible surrogate for SARS-CoV, from surfaces when used as directed. In an outbreak caused by novel agents, it is important to know the effectiveness of disinfectants and antiseptics to prevent or reduce the possibility of human-to-human transmission via surfaces. | |
Přístup | 27. 5. 2020 9:24:37 | |
Publikace | American Journal of Infection Control | |
Jazyk | en | |
Ročník | 37 | |
Číslo | 8 | |
Rozsah | 649-652 | |
ISSN | 0196-6553 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | American Journal of Infection Control | |
DOI | 10.1016/j.ajic.2009.03.012 | |
Select in Zotero | zotero://select/library/items/E3H77ISB | |
Datum přidání | 27. 5. 2020 9:24:37 | |
Upraveno | 5. 3. 2021 10:35:35 |
Autor | Anne-Marie Pagat | |
---|---|---|
Autor | Raphaelle Seux-Goepfert | |
Autor | Charles Lutsch | |
Autor | Valérie Lecouturier | |
Autor | Jean-François Saluzzo | |
Autor | Inca C. Kusters | |
Typ | Článek v časopise | |
Datum | 2007-06-01 | |
Abstrakt | When the first wave of the SARS epidemic seemed to have reached its conclusion, it was completely unclear how the spread of the virus would evolve. Taking into account all the uncertainties and anticipating the worst-case scenario, many laboratories and vaccine manufacturers started working on a vaccine approach against SARS infection. The results presented here describe the evaluation of decontamination practices performed within the framework of a SARS-Coronavirus (SARS-CoV) vaccine development project., We show that it takes 45 days at room temperature to fully inactivate the human SARS-CoV, whereas an enveloped virus such as the rabies virus, when treated in a similar way, is totally inactivated in three days. Moreover, the SARS-CoV is very resistant to alkaline treatment and, even more surprisingly, formaldehyde fumigation is not efficacious on the dried virus (under the conditions tested). Only heat (autoclave) and hypochlorite chloride treatments are efficacious treatments for the decontamination of SARS-CoV. | |
Přístup | 25. 5. 2020 13:06:34 | |
Publikace | Applied Biosafety | |
Jazyk | en | |
Ročník | 12 | |
Číslo | 2 | |
Rozsah | 100-108 | |
Extra | Publisher: SAGE Publications Inc | |
ISSN | 1535-6760 | |
Katalog knihovny | SAGE Journals | |
Zkrácený název časopisu | Appl Biosaf. | |
DOI | 10.1177/153567600701200206 | |
Select in Zotero | zotero://select/library/items/RAYGK7KS | |
Datum přidání | 25. 5. 2020 13:06:34 | |
Upraveno | 5. 3. 2021 10:35:41 |
Autor | Su-Kyung Jo | |
---|---|---|
Autor | Heui-Man Kim | |
Autor | Chang-Jun Lee | |
Autor | Joo-Seob Lee | |
Autor | Sang-Heui Seo | |
Typ | Článek v časopise | |
Datum | 2007-02-13 | |
Abstrakt | Avian influenza viruses cause a considerable threat to humans and animals. In this study, we investigated whether alkaline disinfectant solution can inactivate H5N1, H3N2, H6N1, and H9N2 subtypes of avian influenza virus. When H5N1, H3N2, H6N1, and H9N2 avian influenza viruses were treated with alkaline solution diluted with PBS (pH 7.2) prior to infection into MDCK cells, alkaline disinfectant solution (at dilutions up to <TEX>$10^{-2}$</TEX>) completely inactivated all avian influenza subtypes tested. To confirm the inactivation of avian influenza viruses by alkaline disinfectant solution, we used an immunofluorescence assay with influenza A anti-nucleoprotein antibody and FITC-labeled secondary antibody to stain MDCK cells infected with avian H9N2 influenza viruses. No staining was observed in MDCK rells infected with H9N2 viruses that were pre-treated with a <$10^{-2}$ dilution of alkaline disinfectant solution, while strong staining was observed in MDCK cells infected with H9N2 viruses without pre-treatment. Our results indicate that alkaline solution could help to control avian influenza viruses including the highly pathogenic H5N1 subtype. | |
Přístup | 27. 5. 2020 9:19:23 | |
Publikace | Journal of Life Science | |
Jazyk | eng | |
Ročník | 17 | |
Číslo | 3 | |
Rozsah | 340-344 | |
Extra | Publisher: Korean Society of Life Science | |
ISSN | 1225-9918 | |
Katalog knihovny | www.koreascience.or.kr | |
DOI | 10.5352/JLS.2007.17.3.340 | |
Select in Zotero | zotero://select/library/items/I3C2WX4M | |
Datum přidání | 27. 5. 2020 9:19:23 | |
Upraveno | 5. 3. 2021 10:35:44 |
Autor | Miriam E. R. Darnell | |
---|---|---|
Autor | Deborah R. Taylor | |
Typ | Článek v časopise | |
Datum | 2006-09-21 | |
Abstrakt | BACKGROUND: Severe acute respiratory syndrome coronavirus (SARS-CoV) has been detected in the blood of infected individuals, which may have the potential to contaminate donated blood and plasma-derived products in the event of a future outbreak. Effective methods for inactivating the SARS-CoV in protein solutions are described in this report. STUDY DESIGN AND METHODS: Heat, ultraviolet (UV) irradiation, octanoic acid, and solvent/detergent (S/D) methods were tested individually for their ability to inactivate SARS-CoV in protein solutions appropriately mimicking blood-derived products. Treated samples were tested for inactivation in a tissue culture growth assay. RESULTS: Viral inactivation by heat treatment at 60°C required 15 to 30 minutes to inactivate the SARS-CoV. UVC efficiently inactivated SARS-CoV in 40 minutes, whereas UVA required the addition of psoralen to enhance inactivation of the virus. The presence of bovine serum albumin limited the ability of UVC and UVA to inactivate SARS-CoV and octanoic acid treatment does not reduce the infectivity of SARS-CoV–spiked protein solutions. S/D treatment required 2, 4, and up to 24 hours for Triton X-100, Tween 80, and sodium cholate inactivation, respectively. CONCLUSION: Heat, UVC irradiation, and S/D treatments effectively inactivate SARS-CoV, whereas octanoic acid treatment is insufficient for inactivation of the virus. | |
Přístup | 27. 5. 2020 7:40:54 | |
Publikace | Transfusion | |
Jazyk | en | |
Ročník | 46 | |
Číslo | 10 | |
Rozsah | 1770-1777 | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1537-2995.2006.00976.x | |
ISSN | 1537-2995 | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1111/j.1537-2995.2006.00976.x | |
Select in Zotero | zotero://select/library/items/I49WJP82 | |
Datum přidání | 27. 5. 2020 7:40:54 | |
Upraveno | 5. 3. 2021 10:35:47 |
Autor | Ke-Wen ZHENG | |
---|---|---|
Autor | Qing-Sen YU | |
Autor | Yong-Jun JIANG | |
Autor | Guo-Zheng MA | |
Autor | Min ZEN | |
Typ | Článek v časopise | |
Datum | 2005-01 | |
Abstrakt | The crystal structure of the SARS 3CL proteinase was used to study the electrostatic and hydrophobic interactions between two monomers. Solving the Poisson-Boltzmann equation the finite difference method was used to calculate the electrostatic potential. The solvent accessible surface model was supplied for the molecular surface and hydrophobicity. The electrostatic and hydrophobic interactions were explored under the condition of different pH values. The results indicated that the electrostatic interaction energy, electrostatic desolvation free energy and hydrophobic desolvation free energy showed smaller values when pH values are between 5.5 and 8.5, which indicated that, under the condition, the electrostatic and hydrophobic interaction were favorable to the stability of SARS 3CL proteinase dimer. For the dimer was the active form of the SARS 3CL proteinase, the pH condition was good for retaining the activity of the enzyme. The proteinase had the highest activity at around pH= 7.0 for the strongest electrostatic and hydrophobic interaction, which was consistent with the experimental results. pH values had more influence on the electrostatic desolvation free energy than on the hydrophobic desolvation free energy, which implied that the electrostatic interaction was the key factor to the instability of SARS 3CL proteinase dimer under acidic or alkali condition. | |
URL | http://en.cnki.com.cn/Article_en/CJFDTotal-GDXH200501015.htm | |
Publikace | Chemical Research In Chinese Universities | |
Číslo | 1 | |
Rozsah | 15 | |
Katalog knihovny | Google Scholar | |
Select in Zotero | zotero://select/library/items/T6DUMDWG | |
Datum přidání | 27. 5. 2020 12:39:31 | |
Upraveno | 4. 5. 2021 15:34:29 |
Autor | Miriam E. R. Darnell | |
---|---|---|
Autor | Kanta Subbarao | |
Autor | Stephen M. Feinstone | |
Autor | Deborah R. Taylor | |
Typ | Článek v časopise | |
Datum | 2004-10-01 | |
Abstrakt | Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254nm, heat treatment of 65°C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels. | |
Přístup | 25. 5. 2020 13:34:49 | |
Publikace | Journal of Virological Methods | |
Jazyk | en | |
Ročník | 121 | |
Číslo | 1 | |
Rozsah | 85-91 | |
ISSN | 0166-0934 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Virological Methods | |
DOI | 10.1016/j.jviromet.2004.06.006 | |
Select in Zotero | zotero://select/library/items/LN3M9ULZ | |
Datum přidání | 25. 5. 2020 13:34:49 | |
Upraveno | 5. 3. 2021 10:35:52 |
Autor | Bruce D. Zelus | |
---|---|---|
Autor | Jeanne H. Schickli | |
Autor | Dianna M. Blau | |
Autor | Susan R. Weiss | |
Autor | Kathryn V. Holmes | |
Typ | Článek v časopise | |
Datum | 2003-01-15 | |
Abstrakt | The spike glycoprotein (S) of the murine coronavirus mouse hepatitis virus (MHV) binds to viral murine CEACAM receptor glycoproteins and causes membrane fusion. On virions, the 180-kDa S glycoprotein of the MHV-A59 strain can be cleaved by trypsin to form the 90-kDa N-terminal receptor-binding subunit (S1) and the 90-kDa membrane-anchored fusion subunit (S2). Incubation of virions with purified, soluble CEACAM1a receptor proteins at 37°C and pH 6.5 neutralizes virus infectivity (B. D. Zelus, D. R. Wessner, R. K. Williams, M. N. Pensiero, F. T. Phibbs, M. deSouza, G. S. Dveksler, and K. V. Holmes, J. Virol. 72:7237-7244, 1998). We used liposome flotation and protease sensitivity assays to investigate the mechanism of receptor-induced, temperature-dependent virus neutralization. After incubation with soluble receptor at 37°C and pH 6.5, virions became hydrophobic and bound to liposomes. Receptor binding induced a profound, apparently irreversible conformational change in S on the viral envelope that allowed S2, but not S1, to be degraded by trypsin at 4°C. Various murine CEACAM proteins triggered conformational changes in S on recombinant MHV strains expressing S glycoproteins of MHV-A59 or MHV-4 (MHV-JHM) with the same specificities as seen for virus neutralization and virus-receptor activities. Increased hydrophobicity of virions and conformational change in S2 of MHV-A59 could also be induced by incubating virions at pH 8 and 37°C, without soluble receptor. Surprisingly, the S protein of recombinant MHV-A59 virions with a mutation, H716D, that precluded cleavage between S1 and S2 could also be triggered to undergo a conformational change at 37°C by soluble receptor at neutral pH or by pH 8 alone. A novel 120-kDa subunit was formed following incubation of the receptor-triggered SA59H716D virions with trypsin at 4°C. The data show that unlike class 1 fusion glycoproteins of other enveloped viruses, the murine coronavirus S protein can be triggered to a membrane-binding conformation at 37°C either by soluble receptor at neutral pH or by alkaline pH alone, without requiring previous activation by cleavage between S1 and S2. | |
Přístup | 27. 5. 2020 8:35:53 | |
Publikace | Journal of Virology | |
Jazyk | en | |
Ročník | 77 | |
Číslo | 2 | |
Rozsah | 830-840 | |
Práva | Copyright © 2003 American Society for Microbiology | |
Extra | Publisher: American Society for Microbiology Journals Section: VIRUS-CELL INTERACTIONS PMID: 12502799 | |
ISSN | 1098-5514 | |
Katalog knihovny | jvi.asm.org | |
DOI | 10.1128/JVI.77.2.830-840.2003 | |
Select in Zotero | zotero://select/library/items/MGB3VEVW | |
Datum přidání | 27. 5. 2020 8:35:53 | |
Upraveno | 5. 3. 2021 10:35:54 |
Autor | L. S. Sturman | |
---|---|---|
Autor | C. S. Ricard | |
Autor | K. V. Holmes | |
Typ | Článek v časopise | |
Datum | 1990-06-01 | |
Abstrakt | We have obtained biochemical and electron microscopic evidence of conformational changes at pH 8.0 and 37 degrees C in the coronavirus spike glycoprotein E2 (S). The importance of these changes is reflected in the loss of virus infectivity, the aggregation of virions, and increased virus-induced cell fusion at the same pH. Coronavirus (MHV-A59) infectivity is exquisitely sensitive to pH. The virus was quite stable at pH 6.0 and 37 degrees C (half-life, approximately 24 h) but was rapidly and irreversibly inactivated by brief treatment at pH 8.0 and 37 degrees C (half-life, approximately 30 min). Virions treated at pH 8.0 and 37 degrees C formed clumps and large aggregates. With virions treated at pH 8.0 and 37 degrees C, the amino-terminal peptide E2N (or S1) was released from virions and the remaining peptide, E2C (S2), was aggregated. Viral spikes isolated from detergent-treated virions also aggregated at pH 8.0 and 37 degrees C. Loss of virus infectivity and E2 (S) aggregation at pH 8.0 and 37 degrees C were markedly enhanced in the presence of dithiothreitol. On the basis of the effects of dithiothreitol on the reactions of the peplomer, we propose that release of E2N (S1) and aggregation of E2C (S2) may be triggered by rearrangement of intramolecular disulfide bonds. The aggregation of virions and the isolated E2 (S) glycoprotein at pH 8.0 and 37 degrees C or following treatment with guanidine and urea at pH 6.0 and 37 degrees C indicate that an irreversible conformational change has been induced in the peplomer glycoprotein by these conditions. It is interesting that coronavirus-induced cell fusion also occurred under mildly alkaline conditions and at 37 degrees C. Some enveloped viruses, including influenza viruses and alphaviruses, show conformational changes of spike glycoproteins at a low pH, which correlates with fusion and penetration of those viruses in acidified endocytic vesicles. For coronavirus MHV-A59, comparable conformational change of the spike glycoprotein E2 (S) and cell fusion occurred at a mildly alkaline condition, suggesting that coronavirus infection-penetration, like that of paramyxoviruses and lentiviruses, may occur at the plasma membrane, rather than within endocytic vesicles. | |
Přístup | 27. 5. 2020 8:33:35 | |
URL | https://jvi.asm.org/content/64/6/3042 | |
Publikace | Journal of Virology | |
Jazyk | en | |
Ročník | 64 | |
Číslo | 6 | |
Rozsah | 3042-3050 | |
Extra | Publisher: American Society for Microbiology Journals Section: Research Article PMID: 2159562 | |
ISSN | 1098-5514 | |
Katalog knihovny | jvi.asm.org | |
Select in Zotero | zotero://select/library/items/7ETGD8XQ | |
Datum přidání | 27. 5. 2020 8:33:35 | |
Upraveno | 15. 10. 2020 16:10:05 |
Autor | V. Susan Springthorpe M.Sc | |
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Autor | Syed A. Sattar Ph.D | |
Typ | Článek v časopise | |
Datum | 1990-01-01 | |
Abstrakt | Chemical disinfection is widely practiced as a means of controlling and preventing the spread of infectious diseases. Although disinfection of bacteria has been widely studied, much less attention has been paid to the virucidal potential of commonly used disinfectants in spite of the low infective dose of many human pathogenic viruses. This review considers what is known about the disinfection of viruses and the virucidal properties of different classes of disinfectant chemicals. It focuses on virus disinfection from a practical viewpoint and also critically evaluates the testing techniques currently used for examining the efficacy of disinfectant products. | |
Přístup | 27. 5. 2020 7:18:45 | |
Publikace | Critical Reviews in Environmental Control | |
Ročník | 20 | |
Číslo | 3 | |
Rozsah | 169-229 | |
Extra | Publisher: Taylor & Francis _eprint: https://doi.org/10.1080/10643389009388396 | |
ISSN | 1040-838X | |
Katalog knihovny | Taylor and Francis+NEJM | |
DOI | 10.1080/10643389009388396 | |
Select in Zotero | zotero://select/library/items/CJHHJT9H | |
Datum přidání | 27. 5. 2020 7:18:45 | |
Upraveno | 5. 3. 2021 10:36:02 |
Autor | Alain Lamarre | |
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Autor | Pierre J. Talbot | |
Typ | Článek v časopise | |
Datum | 1989-10-01 | |
Abstrakt | The stability of human coronavirus 229E infectivity was maximum at pH 6.0 when incubated at either 4 or 33 °C. However, the influence of pH was more pronounced at 33 °C. Viral infectivity was completely lost after a 14-day incubation period at 22, 33, or 37 °C but remained relatively constant at 4 °C for the same length of time. Finally, the infectious titer did not show any significant reduction when subjected to 25 cycles of thawing and freezing. These studies will contribute to optimize virus growth and storage conditions, which will facilitate the molecular characterization of this important pathogen.Key words: coronavirus, pH, temperature, infectivity, human coronavirus. | |
Přístup | 26. 5. 2020 6:49:15 | |
Publikace | Canadian Journal of Microbiology | |
Ročník | 35 | |
Číslo | 10 | |
Rozsah | 972-974 | |
Extra | Publisher: NRC Research Press | |
ISSN | 0008-4166 | |
Katalog knihovny | NRC Research Press | |
Zkrácený název časopisu | Can. J. Microbiol. | |
DOI | 10.1139/m89-160 | |
Select in Zotero | zotero://select/library/items/8FST9PIF | |
Datum přidání | 26. 5. 2020 6:49:15 | |
Upraveno | 5. 3. 2021 10:36:05 |
Autor | S. A. Sattar | |
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Autor | V. S. Springthorpe | |
Autor | Y. Karim | |
Autor | P. Loro | |
Typ | Článek v časopise | |
Datum | 1989-06 | |
Abstrakt | The chemical disinfection of virus-contaminated non-porous inanimate surfaces was investigated using coxsackievirus B3, adenovirus type 5, parainfluenzavirus type 3 and coronavirus 229E as representatives of important nosocomial viral pathogens. A 10 µl amount of the test virus, suspended in either faeces or mucin, was placed onto each stainless steel disk (about 1 cm in diameter) and the inoculum allowed to dry for 1 h under ambient conditions. Sixteen disinfectant formulations were selected for this study based on the findings of an earlier investigation with a human rotavirus.After 1 min exposure to 20 µl of the disinfectant, the virus from the disks was immediately eluted into tryptose phosphate broth and plaque assayed. Using an efficacy criterion of a 3 log10 or greater reduction in virus infectivity titre and irrespective of the virus suspending medium, only the following five disinfectants proved to be effective against all the four viruses tested: (1) 2% glutaraldehyde normally used as an instrument soak. (2) a strongly alkaline mixture of 0·5% sodium o-benzyl-p-chlorophenate and 0·6% sodium lauryl sulphate, generally used as a domestic disinfectant cleaner for hard surfaces, (3) a 0·04% solution of a quaternary ammonium compound containing 7% hydrochloric acid, which is the basis of many toilet bowl cleaners. (4) chloraminc T at a minimum free chlorine level of 3000 p.p.m. and (5) sodium hypochlorite at a minimum free chlorine concentration of 5000 p.p.m. Of those chemicals suitable for use as topical antiseptics, 70% ethanol alone or products containing at least 70% ethanol were ineffective only against coxsackievirus B3. These results emphasize the care needed in selecting chemical disinfectants for routine use in infection control. | |
Přístup | 5. 3. 2021 10:38:23 | |
Publikace | Epidemiology & Infection | |
Jazyk | en | |
Ročník | 102 | |
Číslo | 3 | |
Rozsah | 493-505 | |
Extra | Publisher: Cambridge University Press | |
ISSN | 1469-4409, 0950-2688 | |
Katalog knihovny | Cambridge University Press | |
DOI | 10.1017/S0950268800030211 | |
Select in Zotero | zotero://select/library/items/56AC3H66 | |
Datum přidání | 26. 5. 2020 13:40:20 | |
Upraveno | 5. 3. 2021 10:39:13 |