Autor | Te Faye Yap | |
---|---|---|
Autor | Jason C. Hsu | |
Autor | Zhen Liu | |
Autor | Kempaiah Rayavara | |
Autor | Vivian Tat | |
Autor | K. Tseng Chien-Te | |
Autor | Daniel J. Preston | |
Typ | Článek v časopise | |
Datum | 2021-11-07 | |
Abstrakt | Dry heat decontamination has been shown to effectively inactivate viruses without compromising the integrity of delicate personal protective equipment (PPE), allowing safe reuse and helping to alleviate shortages of PPE that have arisen due to COVID-19. Unfortunately, current thermal decontamination guidelines rely on empirical data which are often sparse, limited to a specific virus, and unable to provide fundamental insight into the underlying inactivation reaction. In this work, we experimentally quantified dry heat decontamination of SARS-CoV-2 on disposable masks and validated a model that treats the inactivation reaction as thermal degradation of macromolecules. Furthermore, upon nondimensionalization, all of the experimental data collapse onto a unified curve, revealing that the thermally driven decontamination process exhibits self-similar behavior. Our results show that heating surgical masks to 70 °C for 5minutes inactivates over 99.9% of SARS-CoV-2. We also characterized the chemical and physical properties of disposable masks after heat treatment and did not observe degradation. The model presented in this work enables extrapolation of results beyond specific temperatures to provide guidelines for safe PPE decontamination. The modeling framework and self-similar behavior are expected to extend to most viruses—including yet-unencountered novel viruses—while accounting for a range of environmental conditions. | |
Přístup | 16. 11. 2021 11:56:32 | |
Publikace | Journal of Hazardous Materials | |
Jazyk | en | |
Rozsah | 127709 | |
ISSN | 0304-3894 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Hazardous Materials | |
DOI | 10.1016/j.jhazmat.2021.127709 | |
Select in Zotero | zotero://select/library/items/2UTI7Y49 | |
Datum přidání | 16. 11. 2021 11:56:32 | |
Upraveno | 16. 11. 2021 12:00:04 |
Autor | Isabella Marchesi | |
---|---|---|
Autor | Arianna Sala | |
Autor | Giuseppina Frezza | |
Autor | Stefania Paduano | |
Autor | Sara Turchi | |
Autor | Annalisa Bargellini | |
Autor | Paola Borella | |
Autor | Claudio Cermelli | |
Typ | Článek v časopise | |
Datum | 2021-10-12 | |
Abstrakt | This in vitro study was aimed to assess the efficacy of dry steam in inactivating Human Coronavirus OC43 (HCoV-OC43) as surrogate of SARS-CoV-2, Human Influenza Virus A/H1N1/WSN/33 and Echovirus 7 on stainless steel, polypropylene, and cotton. The virus models were chosen on the basis of their transmission route and environmental resistance. Tests were carried out under a laminar flow cabinet, where two panels of each material were contaminated with a viral suspension. The inocula were left to dry and then the virus on untreated panel (control) was collected by swabbing in order to determine the initial titer. The other panel was treated using a professional vacuum cleaner equipped with a dry steam generator. Dry steam is generated in a boiler where tap water is heated up to 155 °C at 5.5 bar pressure and then during the passage along the flexible hose the temperature decreases to a value between 100 °C and 110 °C at the output. The dry steam was applied for four sec with a window wiper on metal and plastic panels or a brush covered by a microfiber cap on cotton, simulating the steam application during routine cleaning. After the treatment, infectious virus possibly remained on the surface was collected following the same swabbing procedure applied for controls. HCoV-OC43 and Echovirus 7 were titrated by end-point method on HCT-8 line cells and Vero cells, respectively, while Human Influenza Virus was quantified by plaque reduction assay on MDCK cells. Dry steam resulted effective against the three viruses on all tested materials, achieving a mean Log10 reduction factor ≥4 in viral titer of treated samples compared with controls according to UNI EN 14476:2019. Thus, dry steam may be proposed as an ease to use, effective, fast, and nontoxic alternative to chemicals for surface disinfection without damaging materials. Therefore, this device could be employed not only in healthcare facilities but also in occupational, domestic, and community settings, with advantages for environment and human health. | |
Přístup | 16. 11. 2021 11:51:20 | |
Publikace | Journal of Occupational and Environmental Hygiene | |
Ročník | 0 | |
Číslo | 0 | |
Rozsah | 1-6 | |
Extra | Publisher: Taylor & Francis _eprint: https://doi.org/10.1080/15459624.2021.1989442 PMID: 34637686 | |
ISSN | 1545-9624 | |
Katalog knihovny | Taylor and Francis+NEJM | |
DOI | 10.1080/15459624.2021.1989442 | |
Select in Zotero | zotero://select/library/items/W4QNC82J | |
Datum přidání | 16. 11. 2021 11:51:20 | |
Upraveno | 16. 11. 2021 12:00:16 |
Autor | Shan Yan | |
---|---|---|
Autor | Chavis A. Stackhouse | |
Autor | Iradwikanari Waluyo | |
Autor | Adrian Hunt | |
Autor | Kim Kisslinger | |
Autor | Ashley R. Head | |
Autor | David C. Bock | |
Autor | Esther S. Takeuchi | |
Autor | Kenneth J. Takeuchi | |
Autor | Lei Wang | |
Autor | Amy C. Marschilok | |
Typ | Článek v časopise | |
Datum | 2021-10-11 | |
Abstrakt | The COVID-19 pandemic resulted in imminent shortages of personal protective equipment such as face masks. To address the shortage, new sterilization or decontamination procedures for masks are quickly being developed and employed. Dry heat and steam sterilization processes are easily scalable and allow treatment of large sample sizes, thus potentially presenting fast and efficient decontamination routes, which could significantly ease the rapidly increasing need for protective masks globally during a pandemic like COVID-19. In this study, a suite of structural and chemical characterization techniques, including scanning electron microscopy (SEM), contact angle, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Raman were utilized to probe the heat treatment impact on commercially available 3M 8210 N95 Particulate Respirator and VWR Advanced Protection surgical mask. Unique to this study is the use of the synchrotron-based In situ and Operando Soft X-ray Spectroscopy (IOS) beamline (23-ID-2) housed at the National Synchrotron Light Source II at Brookhaven National Laboratory for near-edge X-ray absorption spectroscopy (NEXAFS). | |
Přístup | 16. 11. 2021 11:51:08 | |
Publikace | ACS Sustainable Chemistry & Engineering | |
Krátký název | Reusing Face Covering Masks | |
Ročník | 9 | |
Číslo | 40 | |
Rozsah | 13545-13558 | |
Extra | Publisher: American Chemical Society | |
Katalog knihovny | ACS Publications | |
Zkrácený název časopisu | ACS Sustainable Chem. Eng. | |
DOI | 10.1021/acssuschemeng.1c04530 | |
Select in Zotero | zotero://select/library/items/36G7LSFP | |
Datum přidání | 16. 11. 2021 11:51:08 | |
Upraveno | 16. 11. 2021 12:00:25 |
Autor | Krithika P. Karthigeyan | |
---|---|---|
Autor | Chloe Flanigan | |
Autor | Denis Jacob Machado | |
Autor | Alper A. Kiziltas | |
Autor | Daniel A. Janies | |
Autor | Jay Chen | |
Autor | David Cooke | |
Autor | Marcia V. Lee | |
Autor | Linda J. Saif | |
Autor | Sonny Henegar | |
Autor | Jeff Jahnes | |
Autor | Deborah F. Mielewski | |
Autor | Jesse J. Kwiek | |
Typ | Zpráva | |
Datum | 2021-09-09 | |
Abstrakt | Heat is an established method to inactivate coronaviruses, and there is utility in using heat to reduce viral load on common touch points in vehicles exposed to a person shedding SARS-CoV-2. As SARS-CoV-2 is a Biosafety level (BSL)-3 pathogen, real world testing of heat as a sanitation method for public and private vehicles becomes a challenge, requiring a surrogate coronavirus that can be handled safely outside of a BSL-3 facility. In this study, we used Bovine Coronavirus (BCoV) as a surrogate for SARS-CoV-2 to test the efficacy of heat-based betacoronavirus inactivation. In vitro, a 30-minute exposure to 56°C completely inactivated BCoV in solution, and a 15-minute exposure reduced recovery of BCoV >1000-fold. When heated to 56°C for 15 minutes, the infectivity of BCoV spotted and dried on typical porous and non-porous automobile interior materials was reduced by 99 - 99.99%. When BCoV was spotted and dried on hard plastic (seat) material placed inside an out of service transit bus, 56°C heat for 30 minutes reduced BCoV infectivity 85 - 99.5%. Thus, 56°C is an accessible, rapid, and effective method to inactivate coronaviruses inside motor vehicles. | |
Přístup | 15. 10. 2021 10:10:31 | |
URL | https://www.biorxiv.org/content/10.1101/2021.09.08.459486v1 | |
Jazyk | en | |
Rozsah | 2021.09.08.459486 | |
Práva | © 2021, Posted by Cold Spring Harbor Laboratory. The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author's permission. | |
Extra | Company: Cold Spring Harbor Laboratory DOI: 10.1101/2021.09.08.459486 Distributor: Cold Spring Harbor Laboratory Label: Cold Spring Harbor Laboratory Section: New Results Type: article | |
Katalog knihovny | bioRxiv | |
Select in Zotero | zotero://select/library/items/VQ7DLHC5 | |
Datum přidání | 15. 10. 2021 10:10:31 | |
Upraveno | 15. 10. 2021 10:10:31 |
Autor | Murat Canpolat | |
---|---|---|
Autor | Serhat Bozkurt | |
Autor | Çağrı Şakalar | |
Autor | Ahmet Yılmaz Çoban | |
Autor | Deniz Karaçaylı | |
Autor | Emre Toker | |
Typ | Zpráva | |
Datum | 2021-08-16 | |
Abstrakt | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide with its different variants. The transmission efficiency of the new variants is much higher than the existing ones. Therefore, developing new preventive measures based on the transmission routes of the virus is needed to limit the spread. The possible transmission routes include direct contact with surfaces contaminated with droplets secreted by patients and airborne viral transmission from person to person. Thermal inactivation is a preventive measure that applies high temperature to objects or fluids, as has been reported previously. However, inactivation data of aerosolized SARS-CoV-2 exposed to heat for a short time at high temperatures are not in the literature yet. We evaluated the inactivation of the aerosolized virus while passing through an electric heater. The virus inactivation test experiments were conducted at two temperatures of the heater’s outlet air, 150±5 o C, and 220±5 o C, at an air flow rate of 0.6 m 3 /h (10 L/min) and heat exposure time of 1.44 s. The loss in viability of the virus at 150 o C and 220 o C was measured as 99.900% and 99.999%, respectively. The results indicate that the high-temperature inactivation of SARS-CoV-2 may potentially reduce aerosolized viral indoors. | |
Přístup | 16. 8. 2021 11:04:41 | |
URL | https://www.researchsquare.com/article/rs-552445/v1 | |
Extra | DOI: 10.21203/rs.3.rs-552445/v1 ISSN: 2693-5015 Type: article | |
Katalog knihovny | Research Square | |
Select in Zotero | zotero://select/library/items/4PIYHU7Q | |
Datum přidání | 16. 8. 2021 11:04:41 | |
Upraveno | 16. 8. 2021 14:31:37 |
Autor | Sahar Norouzbeigi | |
---|---|---|
Autor | Reza Yekta | |
Autor | Leily Vahid-Dastjerdi | |
Autor | Hossein Keyvani | |
Autor | Mohammad Mehdi Ranjbar | |
Autor | Mahdi Shadnoush | |
Autor | Mojtaba Yousefi | |
Autor | Nasim Khorshidian | |
Autor | Sara Sohrabvandi | |
Autor | Amir M. Mortazavian | |
Typ | Článek v časopise | |
Datum | 2021-07-20 | |
Abstrakt | The new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that was first found in 2019 in Wuhan, China, caused coronavirus disease 2019 (COVID-19). It then spread worldwide rapidly, causing the 2019–2020 coronavirus pandemic. To date, it has been indicated that various transmission ways might be participated in outbreaks of COVID-19. Among these, food products, whether raw or processed, might be carriers for SARS-CoV-2. Therefore, this study was aimed to evaluate the effect of cooking and microwave process of meat products and bread on the stability of SARS-CoV-2. In this regard, sausages and hamburger as meat products and toast bread were inoculated with a viral load of 5.70 log fifty percent tissue culture infective dose (TCID50)/mL in order to create a simulated cross-contamination condition. The results showed that frying of hamburger at 225ºC for about either 6 or 10 min resulted in complete inactivation of SARS-CoV-2. Furthermore, a 5-log decrease in SARS-CoV-2 load was observed in sausages as a consequence of cooking process at 78ºC for either 20 or 30 min. Additionally, the effect of microwave oven at power of 630 watt on stability of SARS-CoV-2 showed that exposing toast bread for either 30 s or 1 min in this power led to a 5-log decrease in SARS-CoV-2 load in the toast bread. | |
Přístup | 16. 8. 2021 10:50:25 | |
Publikace | Food Science & Nutrition | |
Jazyk | en | |
Ročník | n/a | |
Číslo | n/a | |
Rozsah | fsn3.2481 | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/fsn3.2481 | |
ISSN | 2048-7177 | |
Katalog knihovny | Wiley Online Library | |
Zkrácený název časopisu | Food Sci Nutr | |
DOI | 10.1002/fsn3.2481 | |
Select in Zotero | zotero://select/library/items/DF4JL5YI | |
Datum přidání | 16. 8. 2021 10:50:25 | |
Upraveno | 16. 8. 2021 14:33:20 |
Autor | Tommaso Lomonaco | |
---|---|---|
Autor | Pietro Salvo | |
Autor | Silvia Ghimenti | |
Autor | Denise Biagini | |
Autor | Federico Vivaldi | |
Autor | Andrea Bonini | |
Autor | Roger Fuoco | |
Autor | Fabio Di Francesco | |
Typ | Článek v časopise | |
Datum | 2021-07 | |
Abstrakt | COVID-19 is a highly transmissible respiratory illness that has rapidly spread all over the world causing more than 115 million cases and 2.5 million deaths. Most epidemiological projections estimate that the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus causing the infection will circulate in the next few years and raise enormous economic and social issues. COVID-19 has a dramatic impact on health care systems and patient management, and is delaying or stopping breath research activities due to the risk of infection to the operators following contact with patients, potentially infected samples or contaminated equipment. In this scenario, we investigated whether virus inactivation procedures, based on a thermal treatment (60 degrees C for 1 h) or storage of tubes at room temperature for 72 h, could be used to allow the routine breath analysis workflow to carry on with an optimal level of safety during the pandemic. Tests were carried out using dry and humid gaseous samples containing about 100 representative chemicals found in exhaled breath and ambient air. Samples were collected in commercially available sorbent tubes, i.e. Tenax GR and a combination of Tenax TA, Carbograph 1TD and Carboxen 1003. Our results showed that all compounds were stable at room temperature up to 72 h and that sample humidity was the key factor affecting the stability of the compounds upon thermal treatment. Tenax GR-based sorbent tubes were less impacted by the thermal treatment, showing variations in the range 20%-30% for most target analytes. A significant loss of aldehydes and sulphur compounds was observed using carbon molecular sieve-based tubes. In this case, a dry purge step before inactivation at 60 degrees C significantly reduced the loss of the target analytes, whose variations were comparable to the method variability. Finally, a breath analysis workflow including a SARS-CoV-2 inactivation treatment is proposed. | |
Přístup | 15. 10. 2021 10:03:45 | |
Publikace | Journal of Breath Research | |
Jazyk | English | |
Ročník | 15 | |
Číslo | 3 | |
Rozsah | 037102 | |
Extra | Place: Bristol Publisher: Iop Publishing Ltd WOS:000641030900001 | |
ISSN | 1752-7155 | |
Katalog knihovny | Web of Science Nextgen | |
Zkrácený název časopisu | J. Breath Res. | |
DOI | 10.1088/1752-7163/abf0b4 | |
Select in Zotero | zotero://select/library/items/5TVQHZD3 | |
Datum přidání | 15. 10. 2021 10:03:45 | |
Upraveno | 15. 10. 2021 10:43:20 |
Autor | Jérémy Raiteux | |
---|---|---|
Autor | Marine Eschlimann | |
Autor | Audrey Marangon | |
Autor | Sophie Rogée | |
Autor | Maylis Dadvisard | |
Autor | Laurent Taysse | |
Autor | Guilhem Larigauderie | |
Typ | Článek v časopise | |
Datum | 2021-06-21 | |
Abstrakt | We studied the stability of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) under different simulated outdoor conditions by changing the temperature (20°C and 35°C), the illuminance (darkness, 10 klx, and 56 klx), and/or the cleanness of the surfaces at 50% relative humidity (RH). In darkness, the loss of viability of the virus on stainless steel is temperature dependent, but this is hidden by the effect of the sunlight from the first minutes of exposure. The virus shows a sensitivity to sunlight proportional to the illuminance intensity of the sunlight. The presence of interfering substances has a moderate effect on virus viability even with an elevated illuminance. Thus, SARS-CoV-2 is rapidly inactivated by simulated sunlight in the presence or absence of high levels of interfering substances at 20°C or 35°C and 50% relative humidity. | |
Přístup | 16. 8. 2021 11:01:26 | |
Publikace | Microbiology Spectrum | |
Ročník | 9 | |
Číslo | 1 | |
Rozsah | e00333-21 | |
Extra | Publisher: American Society for Microbiology | |
Katalog knihovny | journals.asm.org (Atypon) | |
DOI | 10.1128/Spectrum.00333-21 | |
Select in Zotero | zotero://select/library/items/MC3ZHHU7 | |
Datum přidání | 16. 8. 2021 11:01:26 | |
Upraveno | 16. 8. 2021 14:33:04 |
Autor | Nilkamal Mahanta | |
---|---|---|
Autor | Varun Saxena | |
Autor | Lalit M. Pandey | |
Autor | Priyanka Batra | |
Autor | U. S. Dixit | |
Typ | Článek v časopise | |
Datum | 2021-06-01 | |
Abstrakt | SARS-CoV-2 virus and other pathogenic microbes are transmitted to the environment through contacting surfaces, which need to be sterilized for the prevention of COVID-19 and related diseases. In this study, a prototype of a cost-effective sterilization box is developed to disinfect small items. The box utilizes ultra violet (UV) radiation with heat. For performance assessment, two studies were performed. First, IgG (glycoprotein, a model protein similar to that of spike glycoprotein of SARS-COV-2) was incubated under UV and heat sterilization. An incubation with UV at 70 °C for 15 min was found to be effective in unfolding and aggregation of the protein. At optimized condition, the hydrodynamic size of the protein increased to ~171 nm from ~5 nm of the native protein. Similarly, the OD280 values also increased from 0.17 to 0.78 indicating the exposure of more aromatic moieties and unfolding of the protein. The unfolding and aggregation of the protein were further confirmed by the intrinsic fluorescence measurement and FTIR studies, showing a 70% increase in the β-sheets and a 22% decrease in the α-helixes of the protein. The designed box was effective in damaging the protein's native structure indicating the effective inactivation of the SARS-COV-2. Furthermore, the incubation at 70 °C for 15 min inside the chamber resulted in 100% antibacterial efficacy for the clinically relevant E.coli bacteria as well as for bacteria collected from daily use items. It is the first detailed performance study on the efficacy of using UV irradiation and heat together for disinfection from virus and bacteria. | |
Přístup | 9. 7. 2021 10:26:32 | |
Publikace | Environmental Research | |
Jazyk | en | |
Ročník | 198 | |
Rozsah | 111309 | |
ISSN | 0013-9351 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Environmental Research | |
DOI | 10.1016/j.envres.2021.111309 | |
Select in Zotero | zotero://select/library/items/GXSE4WS8 | |
Datum přidání | 9. 7. 2021 10:26:32 | |
Upraveno | 9. 7. 2021 12:13:45 |
Autor | Dylan H Morris | |
---|---|---|
Autor | Kwe Claude Yinda | |
Autor | Amandine Gamble | |
Autor | Fernando W Rossine | |
Autor | Qishen Huang | |
Autor | Trenton Bushmaker | |
Autor | Robert J Fischer | |
Autor | M Jeremiah Matson | |
Autor | Neeltje Van Doremalen | |
Autor | Peter J Vikesland | |
Autor | Linsey C Marr | |
Autor | Vincent J Munster | |
Autor | James O Lloyd-Smith | |
Editor | C. Brandon Ogbunugafor | |
Typ | Článek v časopise | |
Datum | 2021-04-27 | |
Abstrakt | Ambient temperature and humidity strongly affect inactivation rates of enveloped viruses, but a mechanistic, quantitative theory of these effects has been elusive. We measure the stability of SARS-CoV-2 on an inert surface at nine temperature and humidity conditions and develop a mechanistic model to explain and predict how temperature and humidity alter virus inactivation. We find SARS-CoV-2 survives longest at low temperatures and extreme relative humidities (RH); median estimated virus half-life is >24 hours at 10C and 40% RH, but ~1.5 hours at 27C and 65% RH. Our mechanistic model uses fundamental chemistry to explain why inactivation rate increases with increased temperature and shows a U-shaped dependence on RH. The model accurately predicts existing measurements of five different human coronaviruses, suggesting that shared mechanisms may affect stability for many viruses. The results indicate scenarios of high transmission risk, point to mitigation strategies, and advance the mechanistic study of virus transmission. | |
Přístup | 3. 5. 2021 13:26:49 | |
Publikace | eLife | |
Ročník | 10 | |
Rozsah | e65902 | |
Extra | Publisher: eLife Sciences Publications, Ltd | |
ISSN | 2050-084X | |
Katalog knihovny | eLife | |
DOI | 10.7554/eLife.65902 | |
Select in Zotero | zotero://select/library/items/RTDEZ988 | |
Datum přidání | 3. 5. 2021 13:26:49 | |
Upraveno | 4. 5. 2021 15:47:27 |
Autor | Christophe Batéjat | |
---|---|---|
Autor | Quentin Grassin | |
Autor | Jean-Claude Manuguerra | |
Autor | India Leclercq | |
Typ | Článek v časopise | |
Datum | 2021-04-14 | |
Abstrakt | Cell culture medium, nasopharyngeal and sera samples spiked with SARS-CoV-2 were subjected to heat inactivation for various periods of time, ranging from 30 s to 60 min. Our results showed that SARS-CoV-2 could be inactivated in less than 30 min, 15 min, and 3 min at 56 °C, 65 °C, and 95 °C, respectively. These data could help laboratory workers to improve their protocols by handling the virus in biosafety conditions. | |
Přístup | 12. 4. 2021 14:31:25 | |
Publikace | Journal of Biosafety and Biosecurity | |
Jazyk | en | |
Ročník | 3 | |
Číslo | 1 | |
Rozsah | 1-3 | |
ISSN | 2588-9338 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Biosafety and Biosecurity | |
DOI | 10.1016/j.jobb.2020.12.001 | |
Select in Zotero | zotero://select/library/items/TP9BGJEG | |
Datum přidání | 12. 4. 2021 14:31:25 | |
Upraveno | 16. 8. 2021 14:13:08 |
Autor | Maria Jose Lista | |
---|---|---|
Autor | Pedro M. Matos | |
Autor | Thomas J. A. Maguire | |
Autor | Kate Poulton | |
Autor | Elena Ortiz-Zapater | |
Autor | Robert Page | |
Autor | Helin Sertkaya | |
Autor | Ana M. Ortega-Prieto | |
Autor | Aoife M. O’Byrne | |
Autor | Clement Bouton | |
Autor | Ruth E. Dickenson | |
Autor | Mattia Ficarelli | |
Autor | Jose M. Jimenez-Guardeño | |
Autor | Mark Howard | |
Autor | Gilberto Betancor | |
Autor | Rui Pedro Galao | |
Autor | Suzanne Pickering | |
Autor | Adrian W. Signell | |
Autor | Harry Wilson | |
Autor | Penelope Cliff | |
Autor | Mark Tan Kia Ik | |
Autor | Amita Patel | |
Autor | Eithne MacMahon | |
Autor | Emma Cunningham | |
Autor | Katie Doores | |
Autor | Monica Agromayor | |
Autor | Juan Martin-Serrano | |
Autor | Esperanza Perucha | |
Autor | Hannah E. Mischo | |
Autor | Manu Shankar-Hari | |
Autor | Rahul Batra | |
Autor | Jonathan Edgeworth | |
Autor | Mark Zuckerman | |
Autor | Michael H. Malim | |
Autor | Stuart Neil | |
Autor | Rocio Teresa Martinez-Nunez | |
Typ | Článek v časopise | |
Datum | 2021-04-11 | |
Abstrakt | There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not have the capacity to develop these and/or perform manual processing. We provide multiple workflows for SARS-CoV-2 nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral RNA Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind Viral DNA/RNA 96 Kit (Omega Bio-tek). We also compared One-step RT-qPCR reagents: TaqMan Fast Virus 1-Step Master Mix (FastVirus, ThermoFisher Scientific), qPCRBIO Probe 1-Step Go Lo-ROX (PCR Biosystems) and Luna® Universal Probe One-Step RT-qPCR Kit (Luna, NEB). We used primer-probes that detect viral N (EUA CDC) and RdRP (PHE guidelines). All RNA extraction methods provided similar results. FastVirus and Luna proved most sensitive. N detection was more reliable than that of RdRP, particularly in samples with low viral titres. Importantly, we demonstrate that treatment of nasopharyngeal swabs with 70 degrees for 10 or 30 min, or 90 degrees for 10 or 30 min (both original variant and B 1.1.7) inactivates SARS-CoV-2 employing plaque assays, and that it has minimal impact on the sensitivity of the qPCR in clinical samples. These findings make SARS-CoV-2 testing portable to settings that do not have CL-3 facilities. In summary, we provide several testing pipelines that can be easily implemented in other laboratories and have made all our protocols and SOPs freely available at https://osf.io/uebvj/. | |
Přístup | 9. 7. 2021 11:59:34 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.04.22.20074351 | |
Práva | © 2021, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution 4.0 International), CC BY 4.0, as described at http://creativecommons.org/licenses/by/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.04.22.20074351 | |
Select in Zotero | zotero://select/library/items/JDDX4L3L | |
Datum přidání | 9. 7. 2021 11:59:34 | |
Upraveno | 9. 7. 2021 12:00:18 |
Autor | Xue Wang | |
---|---|---|
Autor | Shiyi Sun | |
Autor | Boxin Zhang | |
Autor | Jie Han | |
Typ | Článek v časopise | |
Datum | 2021-04-01 | |
Abstrakt | Disinfection is a common practice to inhibit pathogens, yet success is limited by microbial adaptation and our poor knowledge of viral transmission, notably in the current COVID-19 pandemic. There is a need for alternative disinfection strategies and techniques that are adapted to the actual behavior of humans living in densely populated mega-cities. Here, high public circulation in shared passenger vehicles such as taxis, buses and personal cars represents a major risk of viral transmission due to confined space and commonly touched surfaces. Actual regulatory guidelines are not fully successful because they rely both on passengers’ willingness to wear face masks and on drivers’ willingness to disinfect cars after each shift or each ride with symptomatic individuals. Here we propose that passive solar heating, a sustainable technique that has been used in agronomy to kill weeds and soil pathogens, could inactivate the virus in vehicles during warm-to-hot weather within few minutes to half an hour at 50–60 °C. We measured temperatures in a white compact-size sedan left in a parking lot under direct sunlight. Air temperatures increased from 30 to 42–49 °C after 30 min and then reached a plateau at 52–57 °C after 90 min. Temperatures were about 3 °C higher in front versus back of the car and about 5 °C higher at face height compared to knee height. Since COVID-19 is inactivated in 30 min at 56 °C, our findings confirm that hot air generated passively by solar heating in enclosed spaces is a promising strategy of disinfection with benefits of no added costs, chemicals or worktime. Though this technique appears limited to hot climate, possible heating systems that work during parking time might be developed by vehicle makers to extend the technique to cold climates. | |
Přístup | 26. 3. 2021 10:40:50 | |
Publikace | Environmental Chemistry Letters | |
Krátký název | Solar heating to inactivate thermal-sensitive pathogenic microorganisms in vehicles | |
Jazyk | en | |
Ročník | 19 | |
Číslo | 2 | |
Rozsah | 1765-1772 | |
ISSN | 1610-3661 | |
Katalog knihovny | Springer Link | |
Zkrácený název časopisu | Environ Chem Lett | |
DOI | 10.1007/s10311-020-01132-4 | |
Select in Zotero | zotero://select/library/items/6IZ8NGNI | |
Datum přidání | 26. 3. 2021 10:40:50 | |
Upraveno | 9. 7. 2021 12:15:23 |
Autor | Douglas J Perkins | |
---|---|---|
Autor | Robert A Nofchissey | |
Autor | Chunyan Ye | |
Autor | Nathan Donart | |
Autor | Alison Kell | |
Autor | Ivy Foo-Hurwitz | |
Autor | Timothy Muller | |
Autor | Steven B Bradfute | |
Typ | Článek v časopise | |
Datum | 2021-04-01 | |
Abstrakt | The ongoing pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has placed a substantial strain on the supply of personal protective equipment, particularly the availability of N95 respirators for frontline healthcare personnel. These shortages have led to the creation of protocols to disinfect and reuse potentially contaminated personal protective equipment. A simple and inexpensive decontamination procedure that does not rely on the use of consumable supplies is dry heat incubation. Although reprocessing with this method has been shown to maintain the integrity of N95 respirators after multiple decontamination procedures, information on the ability of dry heat incubation to inactivate SARS-CoV-2 is largely unreported. Here, we show that dry heat incubation does not consistently inactivate SARS-CoV-2-contaminated N95 respirators, and that variation in experimental conditions can dramatically affect viability of the virus. Furthermore, we show that SARS-CoV-2 can survive on N95 respirators that remain at room temperature for at least five days. Collectively, our findings demonstrate that dry heat incubation procedures and ambient temperature for five days are not viable methods for inactivating SARS-CoV-2 on N95 respirators for potential reuse. We recommend that decontamination procedures being considered for the reuse of N95 respirators be validated at each individual site and that validation of the process must be thoroughly conducted using a defined protocol. | |
Přístup | 12. 4. 2021 14:27:47 | |
Publikace | Experimental Biology and Medicine | |
Krátký název | COVID-19 global pandemic planning | |
Jazyk | en | |
Ročník | 246 | |
Číslo | 8 | |
Rozsah | 952-959 | |
Extra | Publisher: SAGE Publications | |
ISSN | 1535-3702 | |
Katalog knihovny | SAGE Journals | |
Zkrácený název časopisu | Exp Biol Med (Maywood) | |
DOI | 10.1177/1535370220977819 | |
Select in Zotero | zotero://select/library/items/FFCID9DD | |
Datum přidání | 15. 1. 2021 9:41:13 | |
Upraveno | 9. 7. 2021 12:15:51 |
Autor | Jennifer Biryukov | |
---|---|---|
Autor | Jeremy A. Boydston | |
Autor | Rebecca A. Dunning | |
Autor | John J. Yeager | |
Autor | Stewart Wood | |
Autor | Allison Ferris | |
Autor | David Miller | |
Autor | Wade Weaver | |
Autor | Nathalie E. Zeitouni | |
Autor | Denise Freeburger | |
Autor | Paul Dabisch | |
Autor | Victoria Wahl | |
Autor | Michael C. Hevey | |
Autor | Louis A. Altamura | |
Typ | Článek v časopise | |
Datum | 2021-04-01 | |
Abstrakt | In the absence of a vaccine, preventing the spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the primary means to reduce the impact of the 2019 coronavirus disease (COVID-19). Multiple studies have reported the presence of SARS-CoV-2 genetic material on surfaces suggesting that fomite transmission of SARS-CoV-2 is feasible. High temperature inactivation of virus has been previously suggested, but not shown. In the present study, we investigated the environmental stability of SARS-CoV-2 in a clinically relevant matrix dried onto stainless steel at a high temperature. The results show that at 54.5 °C, the virus half-life was 10.8 ± 3.0 min and the time for a 90% decrease in infectivity was 35.4 ± 9.0 min. These findings suggest that in instances where the environment can reach temperatures of at least 54.5 °C, such as in vehicle interior cabins when parked in warmer ambient air, that the potential for exposure to infectious virus on surfaces could be decreased substantially in under an hour. | |
Přístup | 9. 7. 2021 10:30:10 | |
Publikace | Environmental Chemistry Letters | |
Jazyk | en | |
Ročník | 19 | |
Číslo | 2 | |
Rozsah | 1773-1777 | |
ISSN | 1610-3661 | |
Katalog knihovny | Springer Link | |
Zkrácený název časopisu | Environ Chem Lett | |
DOI | 10.1007/s10311-021-01187-x | |
Select in Zotero | zotero://select/library/items/5AGVSMX2 | |
Datum přidání | 10. 2. 2021 15:35:43 | |
Upraveno | 9. 7. 2021 12:19:24 |
Autor | Jane Burton | |
---|---|---|
Autor | Hannah Love | |
Autor | Kevin Richards | |
Autor | Christopher Burton | |
Autor | Sian Summers | |
Autor | James Pitman | |
Autor | Linda Easterbrook | |
Autor | Katherine Davies | |
Autor | Peter Spencer | |
Autor | Marian Killip | |
Autor | Patricia Cane | |
Autor | Christine Bruce | |
Autor | Allen D. G. Roberts | |
Typ | Článek v časopise | |
Datum | 2021-04-01 | |
Abstrakt | The development of safe diagnostic protocols for working with SARS-CoV-2 clinical samples at Biosafety Level 2 (BSL2) requires understanding of the effect of heat-treatment on SARS-CoV-2 viability and downstream RT-PCR sensitivity. In this study heating SARS-CoV-2/England/2/2020 to 56 °C and 60 °C for 15, 30 and 60 min reduced the virus titre by between 2.1 and 4.9 log10 pfu/mL (as determined by plaque assay). Complete inactivation did not occur and there was significant variability between replicates. Viable virus was detected by plaque assay after heat-treatment at 80 °C for 15 or 30 min but not 60 or 90 min. After heat-treatment at 80 °C for 60 min infectious virus was only detected by more sensitive virus culture. No viable virus was detected after heating to 80 °C for 90 min or 95 °C for 1 or 5 min. RT-PCR sensitivity was not compromised by heating to 56 °C and 60 °C. However, RT-PCR sensitivity was reduced (≥3 Ct value increase) after heating the virus to 80 °C for 30 min or longer, or 95 °C for 1 or 5 min. In summary we found that the efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects downstream is therefore essential for molecular testing. | |
Přístup | 16. 8. 2021 11:03:53 | |
URL | https://www.sciencedirect.com/science/article/pii/S0166093421000264 | |
Publikace | Journal of Virological Methods | |
Jazyk | en | |
Ročník | 290 | |
Rozsah | 114087 | |
ISSN | 0166-0934 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Virological Methods | |
DOI | 10.1016/j.jviromet.2021.114087 | |
Select in Zotero | zotero://select/library/items/XYENHXU5 | |
Datum přidání | 10. 2. 2021 14:50:41 | |
Upraveno | 16. 8. 2021 14:34:12 |
Autor | Amandine Gamble | |
---|---|---|
Autor | Robert J. Fischer | |
Autor | Dylan H. Morris | |
Autor | Kwe Claude Yinda | |
Autor | Vincent J. Munster | |
Autor | James O. Lloyd-Smith | |
Typ | Článek v časopise | |
Datum | 2021-03-24 | |
Abstrakt | Decontamination can limit environmental transmission of infectious agents. It is required for the safe re-use of contaminated medical, laboratory and personal protective equipment, and for the safe handling of biological samples. Heat is widely used for inactivation of infectious agents, notably viruses. We show that for liquid specimens (here, solution of SARS-CoV-2 in cell culture medium), virus inactivation rate under heat treatment at 70°C can vary by almost two orders of magnitude depending on the treatment procedure, from a half-life of 0.86 min (95% credible interval: [0.09, 1.77]) in closed vials in a heat block to 37.00 min ([12.65, 869.82]) in uncovered plates in a dry oven. These findings suggest a critical role of evaporation in virus inactivation via dry heat. Placing samples in open or uncovered containers may dramatically reduce the speed and efficacy of heat treatment for virus inactivation. We conducted a literature review focused on the effect of temperature on coronavirus stability and found that specimen containers, and whether they were closed, covered or uncovered, are rarely reported in the scientific literature. Heat-treatment procedures must be fully specified when reporting experimental studies to facilitate result interpretation and reproducibility, and carefully considered when designing decontamination guidelines. | |
Přístup | 12. 4. 2021 14:15:26 | |
Publikace | bioRxiv | |
Krátký název | Heat-treated virus inactivation rate depends strongly on treatment procedure | |
Jazyk | en | |
Rozsah | 2020.08.10.242206 | |
Práva | © 2021, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Section: New Results | |
Katalog knihovny | www.biorxiv.org | |
DOI | 10.1101/2020.08.10.242206 | |
Select in Zotero | zotero://select/library/items/PKKB7CEV | |
Datum přidání | 12. 4. 2021 14:15:26 | |
Upraveno | 14. 4. 2021 8:27:40 |
Autor | Samuel Faucher | |
---|---|---|
Autor | Daniel James Lundberg | |
Autor | Xinyao Anna Liang | |
Autor | Xiaojia Jin | |
Autor | Rosalie Phillips | |
Autor | Dorsa Parviz | |
Autor | Jacopo Buongiorno | |
Autor | Michael S. Strano | |
Typ | Článek v časopise | |
Datum | 2021-02-28 | |
Abstrakt | While facial coverings reduce the spread of SARS-CoV-2 by viral filtration, masks capable of viral inactivation by heating can provide a complementary method to limit transmission. Inspired by reverse-flow chemical reactors, we introduce a new virucidal face mask concept driven by the oscillatory flow of human breath. The governing heat and mass transport equations are solved to evaluate virus and CO2 transport. Given limits imposed by the kinetics of SARS-CoV-2 thermal inactivation, human breath, safety, and comfort, heated masks may inactivate SARS-CoV-2 to medical-grade sterility. We detail one design, with a volume of 300 mL at 90 °C, that achieves a 3-log reduction in viral load with minimal impedance within the mask mesh, with partition coefficient around 2. This is the first quantitative analysis of virucidal thermal inactivation within a protective face mask, and addresses a pressing need for new approaches for personal protective equipment during a global pandemic. | |
Přístup | 4. 3. 2021 11:05:11 | |
Publikace | AIChE Journal | |
Jazyk | en | |
Ročník | n/a | |
Číslo | n/a | |
Rozsah | e17250 | |
Práva | © 2021 American Institute of Chemical Engineers | |
Extra | _eprint: https://aiche.onlinelibrary.wiley.com/doi/pdf/10.1002/aic.17250 | |
ISSN | 1547-5905 | |
Katalog knihovny | Wiley Online Library | |
Zkrácený název časopisu | AIChE J | |
DOI | 10.1002/aic.17250 | |
Select in Zotero | zotero://select/library/items/DXJBWWW8 | |
Datum přidání | 3. 11. 2020 10:05:55 | |
Upraveno | 26. 3. 2021 13:09:11 |
Comment: 49 pages, 6 figures, 2 supplemental figures
Autor | V. Genoud | |
---|---|---|
Autor | Martín Stortz | |
Autor | A. Waisman | |
Autor | B. G. Berardino | |
Autor | P. Verneri | |
Autor | V. Dansey | |
Autor | Melina Salvatori | |
Autor | F. Remes Lenicov | |
Autor | V. Levi | |
Typ | Článek v časopise | |
Datum | 2021-02-26 | |
Abstrakt | Real-time reverse transcription PCR (RT-qPCR) is the gold-standard technique for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in nasopharyngeal swabs specimens. The analysis by RT-qPCR usually requires a previous extraction step to obtain the purified viral RNA. Unfortunately, RNA extraction constitutes a bottleneck for early detection in many countries since it is expensive, time-consuming and depends on the availability of commercial kits. Here, we describe an extraction-free protocol for SARS-CoV-2 detection by RT-qPCR from nasopharyngeal swab clinical samples in saline solution. The method includes a treatment with proteinase K followed by heat inactivation (PK+HID method). We demonstrate that PK+HID improves the RT-qPCR performance in comparison to the heat-inactivation procedure. Moreover, we show that this extraction-free protocol can be combined with a variety of multiplexing RT-qPCR kits. The method combined with a multiplexing detection kit targeting N and ORF1ab viral genes showed a sensitivity of 0.99 and a specificity of 0.99 from the analysis of 106 positive and 106 negative clinical samples. In conclusion, PK+HID is a robust, fast and inexpensive procedure for extraction-free RT-qPCR determinations of SARS-CoV-2. The National Administration of Drugs, Foods and Medical Devices of Argentina has recently authorized the use of this method. | |
Publikace | PloS one | |
Ročník | 16 | |
Číslo | 2 | |
Katalog knihovny | Semantic Scholar | |
DOI | 10.1371/journal.pone.0247792 | |
Select in Zotero | zotero://select/library/items/YH6ITA24 | |
Datum přidání | 25. 3. 2021 14:27:24 | |
Upraveno | 25. 3. 2021 14:28:38 |
Autor | Yuqian Jiang | |
---|---|---|
Autor | Han Zhang | |
Autor | Jose A. Wippold | |
Autor | Jyotsana Gupta | |
Autor | Jing Dai | |
Autor | Paul de Figueiredo | |
Autor | Julian L. Leibowitz | |
Autor | Arum Han | |
Typ | Článek v časopise | |
Datum | 2021-02-22 | |
Abstrakt | Heat treatment denatures viral proteins that comprise the virion, making the virus incapable of infecting a host. Coronavirus (CoV) virions contain single-stranded RNA genomes with a lipid envelope and four proteins, three of which are associated with the lipid envelope and thus are thought to be easily denatured by heat or surfactant-type chemicals. Prior studies have shown that a temperature as low as 75°C with a treatment duration of 15 min can effectively inactivate CoV. The degree of CoV heat inactivation greatly depends on the length of heat treatment time and the temperature applied. With the goal of finding whether sub-second heat exposure of CoV can sufficiently inactivate CoV, we designed and developed a simple fluidic system that can measure sub-second heat inactivation of CoV. The system is composed of a stainless-steel capillary immersed in a temperature-controlled oil bath followed by an ice bath, through which virus solution can flow at various speeds. Flowing virus solution at different speeds, along with temperature control and monitoring system, allows the virus to be exposed to the desired temperature and treatment durations with high accuracy. Using mouse hepatitis virus, a betacoronavirus, as a model CoV system, we identified that 71.8°C for 0.51 s exposure is sufficient to obtain >5 Log10 reduction in viral titer (starting titer: 5 × 107 PFU/ml), and that when exposed to 83.4°C for 1.03 s, the virus was completely inactivated (>6 Log10 reduction). | |
Přístup | 4. 3. 2021 11:08:33 | |
Publikace | Biotechnology and Bioengineering | |
Jazyk | en | |
Ročník | n/a | |
Číslo | n/a | |
Práva | © 2021 Wiley Periodicals LLC | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.27720 | |
ISSN | 1097-0290 | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1002/bit.27720 | |
Select in Zotero | zotero://select/library/items/F6U7HQLB | |
Datum přidání | 4. 3. 2021 11:08:33 | |
Upraveno | 5. 3. 2021 10:53:48 |
Autor | Catello Scarica | |
---|---|---|
Autor | Lodovico Parmegiani | |
Autor | Laura Rienzi | |
Autor | Attilio Anastasi | |
Autor | Danilo Cimadomo | |
Autor | Francesca Gioia Klinger | |
Autor | Emanuele Licata | |
Autor | Laura Sosa Fernandez | |
Autor | Lucia De Santis | |
Typ | Článek v časopise | |
Datum | 2021-02-05 | |
Abstrakt | Early studies on the newly emerged coronavirus SARS-CoV-2 showed viral particles can remain biologically active on inanimate surfaces, which can potentially act as fomites. However, more knowledge is needed on viral transmission to restrain further spreading of the associated COVID-19. Very recently, Marques and Domingo reviewed the publications on contamination of inert surfaces by SARS-CoV-2, reporting that the virus can survive on surfaces of various materials from hours to a few days [1]. van Doremalen et al. [2] observed that passive SARS-CoV-2 vector transmission can occur through surfaces and aerosol. The same study highlighted that a high viral bioburden can remain infectious for days on surfaces. This report has prompted the adoption of precautions and operational protocols in all medical practices to limit the risk of infection and allow regular access to clinical treatments. Riddel and colleagues [3] produced crucial evidence on survival and stability of SARS-CoV-2 under diverse environmental conditions. According to their data, low temperatures enhance viral survival on surfaces. This knowledge is crucial to improve our strategies of mitigation of the infection risk. Assisted reproductive technology (ART) combines medical and laboratory procedures, to treat both patients and their reproductive cells. Liquid nitrogen (LN2) and nitrogen vapors (NV) are routinely used to cryopreserve gametes, embryos, and gonadal tissues. This is specifically relevant to SARS-CoV-2, as LN2/NV represents a potential risk of cross-contamination [4], carrying viruses and other microorganisms. | |
Přístup | 4. 3. 2021 12:08:54 | |
Publikace | Journal of Assisted Reproduction and Genetics | |
Jazyk | en | |
ISSN | 1573-7330 | |
Katalog knihovny | Springer Link | |
Zkrácený název časopisu | J Assist Reprod Genet | |
DOI | 10.1007/s10815-021-02094-4 | |
Select in Zotero | zotero://select/library/items/KBK68G4T | |
Datum přidání | 4. 3. 2021 12:08:54 | |
Upraveno | 5. 3. 2021 10:53:59 |
Autor | Timsy Uppal | |
---|---|---|
Autor | Amir Khazaieli | |
Autor | Antoine Snijders | |
Autor | Subhash C. Verma | |
Typ | Článek v časopise | |
Datum | 2021-02-02 | |
Abstrakt | The pandemic history of pathogenic SARS-CoV-2 associated COVID-19 infection began in December 2019, with its emergence in Wuhan, China. Pertaining to its high transmissibility and wide host adaptability, this new and unique human coronavirus spread across the planet affecting almost every country, inflicting 91 million people and causing 1.9 million deaths (as of January 17th, 2021). Limited or negligible pre-existing immunity to multiple SARS-CoV-2 variants has resulted in severe morbidity and mortality worldwide, as well as a record-breaking surge in the use of medical-surgical supplies and personal protective equipments. In response to the global need for effective sterilization techniques, this study evaluated the virucidal efficacy of FATHHOME’s self-contained, ozone-based dry-sanitizing device, by dose and time response assessment. We tested inactivation of human coronavirus, HCoV-OC43, a close genetic model of SARS-CoV-2, on porous (N95 filtering facepiece respirator/FFR) and nonporous (glass) surfaces. We started our assays with 20 ppm of ozone for 10 min exposure, which was able to effectively reduce, 99.8% and 99.9% of virus from glass and N95 FFR surfaces, respectively. Importantly, the virus was completely inactivated, below the detection limit (over 6-log10 reduction) with 25 ppm ozone for 15 mins on both tested surfaces. As expected, a higher ozone concentration (50 ppm) resulted in faster inactivation of HCoV-OC43 with 100% inactivation in 10 mins from both the surfaces, with no residual ozone present after completion of the 5-minute post exposure recapture cycle and no measurable increase in ambient ozone levels. These results confirmed that FATHHOME’s device may provide a safe and viable solution for rapid decontamination of SARS-CoV-2- from worn items, frequently touched items, and PPE including N95 FFRs, face shields and other personal items. | |
Přístup | 10. 2. 2021 15:32:56 | |
Krátký název | Inactivation of Human Coronavirus by Fathhome’s Dry Sanitizer Device | |
Jazyk | en | |
Extra | Publisher: Preprints | |
Katalog knihovny | www.preprints.org | |
DOI | 10.20944/preprints202102.0078.v1 | |
Select in Zotero | zotero://select/library/items/UH45XR2P | |
Datum přidání | 10. 2. 2021 15:32:56 | |
Upraveno | 5. 3. 2021 10:54:10 |
Autor | Paul Dabisch | |
---|---|---|
Autor | Michael Schuit | |
Autor | Artemas Herzog | |
Autor | Katie Beck | |
Autor | Stewart Wood | |
Autor | Melissa Krause | |
Autor | David Miller | |
Autor | Wade Weaver | |
Autor | Denise Freeburger | |
Autor | Idris Hooper | |
Autor | Brian Green | |
Autor | Gregory Williams | |
Autor | Brian Holland | |
Autor | Jordan Bohannon | |
Autor | Victoria Wahl | |
Autor | Jason Yolitz | |
Autor | Michael Hevey | |
Autor | Shanna Ratnesar-Shumate | |
Typ | Článek v časopise | |
Datum | 2021-02-01 | |
Abstrakt | Recent evidence suggests that respiratory aerosols may play a role in the spread of SARS-CoV-2 during the ongoing COVID-19 pandemic. Our laboratory has previously demonstrated that simulated sunlight inactivated SARS-CoV-2 in aerosols and on surfaces. In the present study, we extend these findings to include the persistence of SARS-CoV-2 in aerosols across a range of temperature, humidity, and simulated sunlight levels using an environmentally controlled rotating drum aerosol chamber. The results demonstrate that temperature, simulated sunlight, and humidity are all significant factors influencing the persistence of infectious SARS-CoV-2 in aerosols, but that simulated sunlight and temperature have a greater influence on decay than humidity across the range of conditions tested. The time needed for a 90% decrease in infectious virus ranged from 4.8 min at 40 °C, 20% relative humidity, and high intensity simulated sunlight representative of noon on a clear day on the summer solstice at 40°N latitude, to greater than two hours under conditions representative of those expected indoors or at night. These results suggest that the persistence of infectious SARS-CoV-2 in naturally occurring aerosols may be affected by environmental conditions, and that aerosolized virus could remain infectious for extended periods of time under some environmental conditions. The present study provides a comprehensive dataset on the influence of environmental parameters on the survival of SARS-CoV-2 in aerosols that can be utilized, along with data on viral shedding from infected individuals and the inhalational infectious dose, to inform future modeling and risk assessment efforts.Copyright © 2020 American Association for Aerosol Research | |
Přístup | 12. 4. 2021 15:02:30 | |
Publikace | Aerosol Science and Technology | |
Ročník | 55 | |
Číslo | 2 | |
Rozsah | 142-153 | |
Extra | Publisher: Taylor & Francis _eprint: https://doi.org/10.1080/02786826.2020.1829536 | |
ISSN | 0278-6826 | |
Katalog knihovny | Taylor and Francis+NEJM | |
DOI | 10.1080/02786826.2020.1829536 | |
Select in Zotero | zotero://select/library/items/7JLK5LWM | |
Datum přidání | 14. 10. 2020 13:17:26 | |
Upraveno | 9. 7. 2021 12:17:07 |
Autor | Paul Morgan | |
---|---|---|
Autor | Chih-Wen Shu | |
Typ | Zpráva | |
Datum | 2021-01-26 | |
Abstrakt | Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a newly identified RNA virus that causes the serious infection Coronavirus Disease 2019 (COVID-19). The incidence of COVID-19 is still increasing worldwide despite the summer heat and cool winter. However, little is known about seasonal stability of SARS-CoV-2. Herein, we employ Molecular Dynamics (MD) simulations to explore the effect of temperature on four critical SARS-CoV-2 proteins. Our work demonstrates that the spike Receptor Binding Domain (RBD), Main protease (Mpro), and nonstructural protein 3 (macro X) possesses extreme thermos-stability when subjected to temperature variations rendering them attractive drug targets. Furthermore, our findings suggest that these four proteins are well adapted to habitable temperatures on earth and are largely insensitive to cold and warm climates. Furthermore, we report that the critical residues in SARS-CoV-2 RBD were less responsive to temperature variations as compared to the critical residues in SARS-CoV. As such, extreme summer and winter climates, and the transition between the two seasons, are expected to have a negligible effect on the stability of SARS-CoV-2 which will marginally suppress transmission rates until effective therapeutics are available world-wide. | |
Přístup | 16. 8. 2021 14:07:06 | |
URL | https://www.biorxiv.org/content/10.1101/2021.01.24.427990v2 | |
Jazyk | en | |
Rozsah | 2021.01.24.427990 | |
Práva | © 2021, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), CC BY-NC 4.0, as described at http://creativecommons.org/licenses/by-nc/4.0/ | |
Extra | Company: Cold Spring Harbor Laboratory DOI: 10.1101/2021.01.24.427990 Distributor: Cold Spring Harbor Laboratory Label: Cold Spring Harbor Laboratory Section: New Results Type: article | |
Katalog knihovny | bioRxiv | |
Select in Zotero | zotero://select/library/items/FV2G43JU | |
Datum přidání | 16. 8. 2021 14:07:06 | |
Upraveno | 16. 8. 2021 14:07:06 |
Autor | Jie Lin | |
---|---|---|
Autor | Wei Dai | |
Autor | Weiwei Li | |
Autor | Li Xiao | |
Autor | Tao Luo | |
Autor | Yanju Guo | |
Autor | Yang Yang | |
Autor | Ying Han | |
Autor | Peiran Zhu | |
Autor | Qiuyue Wu | |
Autor | Bangshun He | |
Autor | Jian Wu | |
Autor | Xinyi Xia | |
Typ | Článek v časopise | |
Datum | 2021-01-18 | |
Abstrakt | Objectives: With the worldwide spread of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), various antibody detection kits have been developed to test for SARS-CoV-2- specific IgG, IgM, and total antibody. However, the use of different testing methods under various heat-inactivation conditions might affect the COVID-19 detection results. Methods: Seven different antibody detection kits produced by four manufacturers for detection of SARS-CoV-2 IgG, IgM, and total antibody were tested at Wuhan Huoshenshan Hospital, China. Most of the kits used the indirect immunity, capture, and double-antigen sandwich methods. The effects of various heat-inactivation conditions on SARS-CoV-2-specific IgG, IgM, and total antibody detection were analyzed for the different test methods. Results: Using the indirect immunity method, values for SARS-CoV-2 IgG antibody significantly increased and those for IgM antibody decreased with increasing temperature of heat-inactivation using indirect immunity method. However, values for SARS-CoV-2 IgM and total antibody showed no change when the capture and double-antigen sandwich methods were used. The changes in IgG and IgM antibody values with the indirect immunity method indicated that heat-inactivation could affect COVID-19 detection results obtained using this method. In particular, 18 (22.2%) SARS-CoV-2 IgM positive samples were detected as negative with heat-inactivation at 65°C for 30 min, and one (25%) IgG negative sample was detected as positive after heat-inactivation at 56°C for 60 min and 60°C for 30 min. Conclusions: Heat-inactivation could increase SARS-CoV-2 IgG antibody values, and decrease IgM antibody values, causing potential false-positive or false-negative results for COVID-19 antibody detection using the indirect immunity method. Thus, before conducting antibody testing, the testing platforms should be evaluated in accordance with the relevant requirements to ensure accurate COVID-19 detection results. | |
Publikace | Frontiers in Medicine | |
Jazyk | eng | |
Ročník | 7 | |
Extra | PMID: 33537325 PMCID: PMC7849051 | |
ISSN | 2296-858X | |
Katalog knihovny | PubMed | |
Zkrácený název časopisu | Front Med (Lausanne) | |
DOI | 10.3389/fmed.2020.589080 | |
Select in Zotero | zotero://select/library/items/NLW8XT8D | |
Datum přidání | 10. 2. 2021 14:49:33 | |
Upraveno | 5. 3. 2021 10:54:18 |
Autor | Louisa F. Ludwig-Begall | |
---|---|---|
Autor | Constance Wielick | |
Autor | Olivier Jolois | |
Autor | Lorène Dams | |
Autor | Ravo M. Razafimahefa | |
Autor | Hans Nauwynck | |
Autor | Pierre-Francois Demeuldre | |
Autor | Aurore Napp | |
Autor | Jan Laperre | |
Autor | Frédéric Farnir | |
Autor | Etienne Thiry | |
Autor | Eric Haubruge | |
Typ | Článek v časopise | |
Datum | 2021-01-15 | |
Abstrakt | As the SARS-CoV-2 pandemic accelerates, the supply of personal protective equipment remains under strain. To combat shortages, re-use of surgical masks and filtering facepiece respirators has been recommended. Prior decontamination is paramount to the re-use of these typically single-use only items and, without compromising their integrity, must guarantee inactivation of SARS-CoV-2 and other contaminating pathogens. Aim We provide information on the effect of time-dependent passive decontamination at room temperature and evaluate inactivation of a SARS-CoV-2 surrogate and a non-enveloped model virus as well as mask and respirator integrity following active multiple-cycle vaporised hydrogen peroxide (VHP), ultraviolet germicidal irradiation (UVGI), and dry heat (DH) decontamination. Methods Masks and respirators, inoculated with infectious porcine respiratory coronavirus or murine norovirus, were submitted to passive decontamination or single or multiple active decontamination cycles; viruses were recovered from sample materials and viral titres were measured via TCID<sub>50</sub> assay. In parallel, filtration efficiency tests and breathability tests were performed according to EN standard 14683 and NIOSH regulations. Results and Discussion Infectious porcine respiratory coronavirus and murine norovirus remained detectable on masks and respirators up to five and seven days of passive decontamination. Single and multiple cycles of VHP-, UVGI-, and DH were shown to not adversely affect bacterial filtration efficiency of masks. Single- and multiple UVGI did not adversely affect respirator filtration efficiency, while VHP and DH induced a decrease in filtration efficiency after one or three decontamination cycles. Multiple cycles of VHP-, UVGI-, and DH slightly decreased airflow resistance of masks but did not adversely affect respirator breathability. VHP and UVGI efficiently inactivated both viruses after five, DH after three, decontamination cycles, permitting demonstration of a loss of infectivity by more than three orders of magnitude. This multi-disciplinal approach provides important information on how often a given PPE item may be safely reused. | |
Přístup | 25. 3. 2021 14:21:27 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2021.01.15.21249866 | |
Práva | © 2021, Posted by Cold Spring Harbor Laboratory. The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author's permission. | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2021.01.15.21249866 | |
Select in Zotero | zotero://select/library/items/TTTDPVE8 | |
Datum přidání | 25. 3. 2021 14:21:27 | |
Upraveno | 14. 4. 2021 8:31:35 |
Autor | Heidi Auerswald | |
---|---|---|
Autor | Sokhoun Yann | |
Autor | Sokha Dul | |
Autor | Saraden In | |
Autor | Philippe Dussart | |
Autor | Nicholas J. Martin | |
Autor | Erik A. Karlsson | |
Autor | Jose A. Garcia-Rivera | |
Typ | Článek v časopise | |
Datum | 2021-01-08 | |
Abstrakt | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), presents a challenge to laboratorians and healthcare workers around the world. Handling of biological samples from individuals infected with the SARS-CoV-2 virus requires strict biosafety measures. Within the laboratory, non-propagative work with samples containing the virus requires, at minimum, Biosafety Level-2 (BSL-2) techniques and facilities. Therefore, handling of SARS-CoV-2 samples remains a major concern in areas and conditions where biosafety for specimen handling is difficult to maintain, such as in rural laboratories or austere field testing sites. Inactivation through physical or chemical means can reduce the risk of handling live virus and increase testing ability especially in low-resource settings due to easier and faster sample processing. Herein we assess several chemical and physical inactivation techniques employed against SARS-CoV-2 isolates from Cambodia. This data demonstrates that all chemical (AVL, inactivating sample buffer and formaldehyde) and heat-treatment (56 and 98 °C) methods tested completely inactivated viral loads of up to 5 log10. | |
Publikace | The Journal of General Virology | |
Jazyk | eng | |
Extra | PMID: 33416462 | |
ISSN | 1465-2099 | |
Katalog knihovny | PubMed | |
Zkrácený název časopisu | J Gen Virol | |
DOI | 10.1099/jgv.0.001539 | |
Select in Zotero | zotero://select/library/items/EZX242WP | |
Datum přidání | 10. 2. 2021 14:53:42 | |
Upraveno | 5. 3. 2021 10:54:25 |
Autor | Jill van Kessel | |
---|---|---|
Typ | Článek v časopise | |
Datum | 2021-01-01 | |
Abstrakt | Objective: Biosecurity in swine transport trailers is of concern for spreading pathogens between premises, and as such, they require extensive cleaning and disinfection between loads. Our goal in this study was to find the optimal time and temperature required to heat inactivate swine pathogens of high concern to producers in a laboratory setting to then be extrapolated to transport trailers. Materials and methods: Using standard microbiological techniques for growth and purification, 5 bacterial and 5 viral pathogens important in swine health were produced and tested. Heat inactivation of these pathogens were tested in the lab using several time and temperature combinations. Fecal matter was added to test the effect of biological material on the time and temperatures required for inactivation. Results: Inactivation was complete for viruses and bacteria tested when heated to 75°C for 15 minutes. The presence of fecal matter resulted in increased time and temperature needed for pathogen inactivation. Implications: Heat baking of transport trailers is now being applied as a useful tool to reduce the transmission of pathogens commonly associated with swine disease. However, operators must ensure consistent heating to 75°C for a minimum of 15 minutes in all areas of the trailer for reliable inactivation. Cleaning trailers plays an important role prior to heat treatment, as the presence of fecal contamination will insulate the pathogens and inactivation may not be complete even at 75°C for 15 minutes. | |
URL | https://www.aasv.org/shap/issues/v29n1/v29n1p19.html | |
Publikace | Journal of Swine Health and Production | |
Ročník | 29 | |
Číslo | 1 | |
Rozsah | 19-28 | |
ISSN | 1066-4963 | |
Zkrácený název časopisu | JSHAP | |
Select in Zotero | zotero://select/library/items/4D4DF24K | |
Datum přidání | 15. 1. 2021 11:23:55 | |
Upraveno | 9. 7. 2021 12:13:29 |
Autor | A. Sharma | |
---|---|---|
Autor | B. Preece | |
Autor | H. Swann | |
Autor | X. Fan | |
Autor | R. J. McKenney | |
Autor | K. M. Ori-McKenney | |
Autor | S. Saffarian | |
Autor | M. D. Vershinin | |
Typ | Článek v časopise | |
Datum | 2021-01-01 | |
Abstrakt | SARS-CoV-2 is a novel coronavirus which has caused the COVID-19 pandemic. Other known coronaviruses show a strong pattern of seasonality, with the infection cases in humans being more prominent in winter. Although several plausible origins of such seasonal variability have been proposed, its mechanism is unclear. SARS-CoV-2 is transmitted via airborne droplets ejected from the upper respiratory tract of the infected individuals. It has been reported that SARS-CoV-2 can remain infectious for hours on surfaces. As such, the stability of viral particles both in liquid droplets as well as dried on surfaces is essential for infectivity. Here we have used atomic force microscopy to examine the structural stability of individual SARS-CoV-2 virus like particles at different temperatures. We demonstrate that even a mild temperature increase, commensurate with what is common for summer warming, leads to dramatic disruption of viral structural stability, especially when the heat is applied in the dry state. This is consistent with other existing non-mechanistic studies of viral infectivity, provides a single particle perspective on viral seasonality, and strengthens the case for a resurgence of COVID-19 in winter. | |
Přístup | 12. 4. 2021 14:31:25 | |
Publikace | Biochemical and Biophysical Research Communications | |
Jazyk | en | |
Ročník | 534 | |
Rozsah | 343-346 | |
ISSN | 0006-291X | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Biochemical and Biophysical Research Communications | |
DOI | 10.1016/j.bbrc.2020.11.080 | |
Select in Zotero | zotero://select/library/items/9IBVEFIN | |
Datum přidání | 16. 12. 2020 11:02:30 | |
Upraveno | 14. 4. 2021 8:32:13 |
Autor | Qi Lv | |
---|---|---|
Autor | Mingya Liu | |
Autor | Feifei Qi | |
Autor | Shuran Gong | |
Autor | Shasha Zhou | |
Autor | Shisheng Zhan | |
Autor | Linlin Bao | |
Typ | Článek v časopise | |
Datum | 2020-12-29 | |
Abstrakt | This study was designed to investigate the sensitivity of SARS-CoV-2 to different temperatures, to provide basic data and a scientific basis for the control of COVID-19 epidemic. The virus was dispersed in 1 mL basal DMEM medium at a final concentration of 103.2 TCID50/mL and then incubated at 4, 22, 30, 35, 37, 38, 39 and 40°C for up to 5 days. The infectivity of residual virus was titrated using the Vero E6 cell line. The results showed that the virus remained viable for 5 days at 4°C, and for 1 day only at 22 and 30°C. We found that the infectivity of the virus was completely lost after less than 12 hours at 37, 38 and 39°C, while at 40°C, the inactivation time of the virus was rapidly reduced to 6 hours. We show that SARS-CoV-2 is sensitive to heat, is more stable at lower temperatures than higher temperature, remains viable for longer at lower temperatures, and loses viability rapidly at higher temperatures. | |
Přístup | 15. 1. 2021 10:48:26 | |
Publikace | Animal Models and Experimental Medicine | |
Jazyk | en | |
Ročník | n/a | |
Číslo | n/a | |
Práva | © 2020 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/ame2.12141 | |
ISSN | 2576-2095 | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1002/ame2.12141 | |
Select in Zotero | zotero://select/library/items/BY9Y8IZB | |
Datum přidání | 15. 1. 2021 10:48:26 | |
Upraveno | 5. 3. 2021 10:54:45 |
Autor | Y. W. Choi | |
---|---|---|
Autor | A. W. Richardson | |
Autor | M. Sunderman | |
Autor | M. J. Mladineo | |
Autor | P. H. Keyes | |
Autor | K. C. Hofacre | |
Autor | J. K. Middleton | |
Typ | Článek v časopise | |
Datum | 2020-12-21 | |
Abstrakt | Decontamination of N95 filtering facepiece respirators (FFRs) is a crisis capacity strategy allowed when there are known shortages of FFRs. The application of moist heat is one decontamination method that has shown promise and is the approach approved in the Steris Steam Emergency Use Authorization (EUA). This effort examines the use of multicookers to apply moist heat, as they are available in retail stores and more affordable than methods requiring more sophisticated equipment. Four of five multicooker models examined met the acceptance criteria for the test and one model was selected for inactivation testing. Tests were performed on four different FFR models with SARS-CoV-2 suspended in culture media, simulated saliva or simulated lung fluid. Moist heat treatment reduced recoverable titres of SARS-CoV-2 virus to levels below the limit of detection in all tests. Furthermore, these four FFR models showed no loss in collection efficiency, inhalation resistance or visual damage after up to 10 decontamination cycles. Two (2) FFR models showed a slight change in strap elasticity (<9%). These data show that moist heat treatment using a multicooker is a viable option for FFR decontamination in a crisis capacity strategy. | |
Přístup | 15. 1. 2021 11:02:21 | |
Publikace | Letters in Applied Microbiology | |
Jazyk | en | |
Ročník | n/a | |
Číslo | n/a | |
Práva | © 2020 The Authors. Letters in Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology. | |
Extra | _eprint: https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/lam.13443 | |
ISSN | 1472-765X | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1111/lam.13443 | |
Select in Zotero | zotero://select/library/items/KL4938AA | |
Datum přidání | 15. 1. 2021 11:02:21 | |
Upraveno | 5. 3. 2021 10:54:54 |
Autor | G. Singh | |
---|---|---|
Autor | J. Jorgenson | |
Autor | T. Pringle | |
Autor | T. Nelson | |
Autor | S. Ramamoorthy | |
Typ | Článek v časopise | |
Datum | 2020-12-16 | |
Abstrakt | The critical need for reliable methods to validate decontamination protocols for personal protective equipment (PPE) for re-use during the SARS-CoV-2 pandemic is limited by the need for specialized containment facilities to handle the virus. Hence, we have herein validated the use of a swine coronavirus as a surrogate, and tested the effectiveness of dry heat and ultraviolet (UV) rays for PPE decontamination. Exposure of experimentally contaminated N95 masks and hospital gowns to 60°C for 20 min, and UVC at 1800 mJ/cm2 resulted in a 4-log reduction and inactivation of the surrogate virus. This study provides a novel alternative to validate PPE reprocessing methods. | |
Přístup | 16. 12. 2020 10:50:08 | |
Publikace | Infection Prevention in Practice | |
Jazyk | en | |
Ročník | 3 | |
Číslo | 1 | |
Rozsah | 100103 | |
ISSN | 2590-0889 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Infection Prevention in Practice | |
DOI | 10.1016/j.infpip.2020.100103 | |
Select in Zotero | zotero://select/library/items/HNGJ2RH2 | |
Datum přidání | 16. 12. 2020 10:50:08 | |
Upraveno | 9. 7. 2021 12:16:17 |
Autor | Fatemeh Saadatpour | |
---|---|---|
Autor | Fatemeh Mohammadipanah | |
Typ | Článek v časopise | |
Datum | 2020-12-02 | |
Abstrakt | The transmission control of the newly emerged severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is the most effective strategy by the absence of its specified vaccine or drug. Although the aerosol mediated transmission of SARS‐CoV‐2 has been confirmed, the physicochemical treatment of the biotic and abiotic objects is still the most promising approach in its infection control. The front line of the most effective disinfecting compounds on SARS‐CoV‐2 implies to be sodium hypochlorite, ethanol, hydrogen peroxide, quaternary ammonium compounds, and phenolic compounds, respectively. However, widely used compounds of alkyldimethylbenzylammonium chloride (benzalkonium chloride) biguanides (chlorhexidine) have not shown the multitude load reduction in less than 10 minutes. The susceptibility of SARS‐CoV‐2 to physical treatment follows the pattern of heat, acidity, and UV radiation. Rather all of the mentioned physical or chemical treatments, target the envelope proteins of the coronavirus mainly by impairing its entry to host cells. The anti‐SARS‐CoV‐2 activity of combinatorial physicochemical treatments or evaluation of new chemical entities or physical treatments such as microwave irradiation still needs to be explored. Therefore, the development of a reliable decontamination protocol for SARS‐CoV‐2 demands revealing its stability pattern study vs a spectrum of single and combinatorial physicochemical parameters. | |
Publikace | Health Science Reports | |
Jazyk | English | |
Ročník | 3 | |
Číslo | 4 | |
Archiv | Coronavirus Research Database | |
Místo v archivu | 2466347748 | |
Extra | Place: Hoboken Publisher: John Wiley & Sons, Inc. | |
DOI | 10.1002/hsr2.213 | |
Select in Zotero | zotero://select/library/items/39N4PQ43 | |
Datum přidání | 14. 12. 2020 14:13:24 | |
Upraveno | 5. 3. 2021 10:33:17 |
Copyright - © 2020. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright - © 2020. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Název - Environmental Protection Agency--EPA
Poslední aktualizace - 2020-12-03
Poslední aktualizace - 2020-12-14
SubjectsTermNotLitGenreText - Middle East
Autor | Shahar Seifer | |
---|---|---|
Autor | Michael Elbaum | |
Typ | Článek v časopise | |
Datum | 2020-11-28 | |
Abstrakt | Based on a model of protein denaturation rate-limited by an entropy-related barrier, we derive a simple formula for virus inactivation time as a function of temperature. Loss of protein structure is described by two reaction coordinates: conformational disorder of the polymer and wetting by the solvent. These establish a competition between conformational entropy and hydrophobic interaction favoring random coil or globular states, respectively. Based on the Landau theory of phase transition, the resulting free energy barrier is found to decrease linearly with the temperature difference T-Tm, and the inactivation rate should scale as U to the power of T-Tm. This form recalls an accepted model of thermal damage to cells in hyperthermia. For SARS-CoV-2 the value of U in Celsius units is found to be 1.32. Although the fitting of the model to measured data is practically indistinguishable from Arrhenius law with an activation energy, the entropy barrier mechanism is more suitable and could explain the pronounced sensitivity of SARS-CoV-2 to thermal damage. Accordingly, we predict the efficacy of mild fever over a period of about 24 hours in inactivating the virus. | |
Přístup | 16. 12. 2020 11:09:39 | |
Publikace | Biophysical Journal | |
Jazyk | en | |
ISSN | 0006-3495 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Biophysical Journal | |
DOI | 10.1016/j.bpj.2020.11.2259 | |
Select in Zotero | zotero://select/library/items/BD9MYI4V | |
Datum přidání | 16. 12. 2020 11:09:39 | |
Upraveno | 5. 3. 2021 10:55:19 |
Autor | Hyesun Jang | |
---|---|---|
Autor | Ted M. Ross | |
Typ | Článek v časopise | |
Datum | 2020-11-23 | |
Abstrakt | Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) is a great concern on both public and veterinary health. Multiple studies showed that the SARS-CoV-2 can persist for few days in wet condition, but it has not been clear whether the virus can maintain the infectivity in dry condition. Thus, we measured the infectious titer of dried SARS-CoV-2 (104 pfu/25 μL droplet) at 0, 0.5, 1, 2, 3, 24, and 48 h. Strikingly, the dried SARS-CoV-2 virus did not lose the infectivity to Vero E6 cells for up to 48 h. Our findings warrants that the drying cannot replace the surface disinfection to prevent transmission via common vehicle or nosocomial infection. Future studies can apply our experimental setting to test the efficacy of diverse disinfecting procedures. | |
Přístup | 23. 11. 2020 14:25:49 | |
Publikace | Veterinary Microbiology | |
Jazyk | en | |
Ročník | 251 | |
Rozsah | 108907 | |
ISSN | 0378-1135 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Veterinary Microbiology | |
DOI | 10.1016/j.vetmic.2020.108907 | |
Select in Zotero | zotero://select/library/items/X2V6QDCG | |
Datum přidání | 23. 11. 2020 14:25:49 | |
Upraveno | 5. 3. 2021 10:55:25 |
Autor | Giancarlo la Marca | |
---|---|---|
Autor | Jacopo Barp | |
Autor | Stefano Frenos | |
Autor | Alessandro Mugelli | |
Autor | Luisa Galli | |
Autor | Elisa Calistri | |
Autor | Giacomo Biasucci | |
Autor | Salvatore De Masi | |
Autor | Renzo Guerrini | |
Typ | Článek v časopise | |
Datum | 2020-11-21 | |
Abstrakt | Background The emergence of SARS-CoV-2 pandemic has upset health systems around the world and caused immeasurable losses and costs. Until a vaccine will become available, the recommended prevention measures remain physical distancing and enhanced hygiene. Methods and findings The proteic structure external to the virus is the main target that may eventually lead to reduce or block its replication in the upper airways. We developed a protocol based of repeated steam inhalation cycles aimed at reducing the risk of progression to full blown infection if performed soon after contagion. The protocol has been used in a single-center open label trial on ten infected asymptomatic or pauci-symptomatic health care professionals. Conclusions The promising results we obtained with this easily accessible, non-invasive and inexpensive procedure should prompt controlled trials. | |
Přístup | 23. 11. 2020 14:22:23 | |
Publikace | Life Sciences | |
Krátký název | Thermal inactivation of SARS COVID-2 virus | |
Jazyk | en | |
Rozsah | 118801 | |
ISSN | 0024-3205 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Life Sciences | |
DOI | 10.1016/j.lfs.2020.118801 | |
Select in Zotero | zotero://select/library/items/UZCTY3GQ | |
Datum přidání | 23. 11. 2020 14:22:23 | |
Upraveno | 5. 3. 2021 10:55:34 |
Autor | Max Bueckert | |
---|---|---|
Autor | Rishi Gupta | |
Autor | Aditi Gupta | |
Autor | Mohit Garg | |
Autor | Asit Mazumder | |
Typ | Článek v časopise | |
Datum | 2020-11-18 | |
Abstrakt | The unwavering spread of COVID-19 has taken the world by storm. Preventive measures like social distancing and mask usage have been taken all around the globe but still, as of September 2020, the number of cases continues to rise in many countries. Evidently, these measures are insufficient. Although decreases in population density and surges in the public’s usage of personal protective equipment can mitigate direct transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), indirect transmission of the virus is still probable. By summarizing the current state of knowledge on the stability of coronaviruses on dry materials, this review uncovers the high potential for SARS-CoV-2 transmission through contaminated surfaces (i.e., fomites) and prompts future research. Fully contextualized data on coronavirus persistence are presented. The methods and limitations to testing the stability of coronaviruses are explored, and the SARS-CoV-2 representativeness of different coronaviruses is analyzed. The factors which dictate the persistence of coronaviruses on surfaces (media, environmental conditions, and material-type) are investigated, and the review is concluded by encouraging material innovation to combat the current pandemic. To summarize, SARS-CoV-2 remains viable on the timescale of days on hard surfaces under ambient indoor conditions. Similarly, the virus is stable on human skin, signifying the necessity of hand hygiene amidst the current pandemic. There is an inverse relationship between SARS-CoV-2 surface persistence and temperature/humidity, and the virus is well suited to air-conditioned environments (room temperature, ~ 40% relative humidity). Sunlight may rapidly inactivate the virus, suggesting that indirect transmission predominantly occurs indoors. The development of antiviral materials and surface coatings would be an extremely effective method to mitigate the spread of COVID-19. To obtain applicable data on the persistence of coronaviruses and the efficiency of virucidal materials, future researchers should understand the common experimental limitations outlined in this review and plan their studies accordingly. | |
Přístup | 16. 12. 2020 16:04:40 | |
Publikace | Materials | |
Krátký název | Infectivity of SARS-CoV-2 and Other Coronaviruses on Dry Surfaces | |
Jazyk | en | |
Ročník | 13 | |
Číslo | 22 | |
Rozsah | 5211 | |
Práva | http://creativecommons.org/licenses/by/3.0/ | |
Extra | Number: 22 Publisher: Multidisciplinary Digital Publishing Institute | |
Katalog knihovny | www.mdpi.com | |
DOI | 10.3390/ma13225211 | |
Select in Zotero | zotero://select/library/items/68S4E6CU | |
Datum přidání | 16. 12. 2020 16:04:40 | |
Upraveno | 13. 4. 2021 14:54:46 |
Autor | Dylan H. Morris | |
---|---|---|
Autor | Kwe Claude Yinda | |
Autor | Amandine Gamble | |
Autor | Fernando W. Rossine | |
Autor | Qishen Huang | |
Autor | Trenton Bushmaker | |
Autor | Robert J. Fischer | |
Autor | M. Jeremiah Matson | |
Autor | Neeltje van Doremalen | |
Autor | Peter J. Vikesland | |
Autor | Linsey C. Marr | |
Autor | Vincent J. Munster | |
Autor | James O. Lloyd-Smith | |
Typ | Článek v časopise | |
Datum | 2020-11-13 | |
Abstrakt | Understanding the impact of environmental conditions on virus viability and transmission potential is crucial to anticipating epidemic dynamics and designing mitigation strategies. Ambient temperature and humidity are known to have strong effects on the environmental stability of viruses, but a general quantitative understanding of how temperature and humidity affect virus stability has remained elusive. We characterize the stability of SARS-CoV-2 on an inert surface at a variety of temperature and humidity conditions, and introduce a mechanistic model that enables accurate prediction of virus stability in unobserved conditions. We find that SARS-CoV-2 survives better at low temperatures and extreme relative humidities; median estimated virus half-life was more than 24 hours at 10C and 40% RH, but approximately an hour and a half at 27C and 65% RH. Moreover, our model predicts observations from other human coronaviruses and other studies of SARS-CoV-2, suggesting the existence of shared mechanisms that determine environmental stability across a number of enveloped viruses. Our results highlight scenarios of particular transmission risk and point to broad strategies for pandemic mitigation, while opening new frontiers for the mechanistic study of viral transmission. | |
Přístup | 23. 11. 2020 15:00:48 | |
Publikace | bioRxiv | |
Jazyk | en | |
Rozsah | 2020.10.16.341883 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author's permission. | |
Extra | Publisher: Cold Spring Harbor Laboratory Section: New Results | |
Katalog knihovny | www.biorxiv.org | |
DOI | 10.1101/2020.10.16.341883 | |
Select in Zotero | zotero://select/library/items/YMDSCSIR | |
Datum přidání | 23. 11. 2020 15:00:48 | |
Upraveno | 5. 3. 2021 10:55:50 |
Autor | Jan Schinköthe | |
---|---|---|
Autor | Hendrik A. Scheinemann | |
Autor | Sandra Diederich | |
Autor | Holger Freese | |
Autor | Michael Eschbaumer | |
Autor | Jens P. Teifke | |
Autor | Sven Reiche | |
Typ | Článek v časopise | |
Datum | 2020-11-06 | |
Abstrakt | Airborne disinfection of high-containment facilities before maintenance or between animal studies is crucial. Commercial spore carriers (CSC) coated with 106 spores of Geobacillus stearothermophilus are often used to assess the efficacy of disinfection. We used quantitative carrier testing (QCT) procedures to compare the sensitivity of CSC with that of surrogates for non-enveloped and enveloped viruses including SARS-CoV-2, mycobacteria, and spores to an aerosolized mixture of peroxyacetic acid and hydrogen peroxide (aPAA/HP). We then used the QCT methodology to determine relevant process parameters to develop and validate effective disinfection protocols (≥ 4 log10 reduction) in various large and complex facilities. Our results demonstrate that aPAA/HP is a highly efficient procedure for airborne room disinfection. Relevant process parameters such as temperature and relative humidity can be wirelessly monitored. Furthermore, we found striking differences in inactivation efficacies against some of the tested microorganisms. Overall, we conclude that dry fogging a mixture of aPAA/HP is highly efficient against a broad range of microorganisms as well as material compatible with relevant concentrations. Furthermore, CSC are artificial bio-indicators with lower resistance and thus, should not be used for validating airborne disinfection when microorganisms other than viruses have to be inactivated. IMPORTANCE Airborne disinfection is not only of crucial importance for the safe operation of laboratories and animal rooms where infectious agents are handled, but can also be used in public health emergencies like the current SARS-CoV-2 pandemic. We show that dry fogging a mixture of aPAA/HP is highly microbicidal, efficient, fast, robust, environmentally neutral, and a suitable airborne disinfection method. In addition, the low concentration of dispersed disinfectant, particularly for enveloped viral pathogens like SARS-CoV-2, entails high material compatibility. For these reasons and due to the relative simplicity of the procedure, it is an ideal disinfection method for hospital wards, ambulances, public conveyances and indoor community areas. Thus, we conclude that this method is an excellent choice for control of the current SARS-CoV-2 pandemic. | |
Přístup | 23. 11. 2020 14:57:10 | |
Publikace | Applied and Environmental Microbiology | |
Jazyk | en | |
Práva | Copyright © 2020 American Society for Microbiology.. All Rights Reserved. | |
Extra | Publisher: American Society for Microbiology Section: Environmental Microbiology PMID: 33158901 | |
ISSN | 1098-5336 | |
Katalog knihovny | aem.asm.org | |
Zkrácený název časopisu | Appl. Environ. Microbiol. | |
DOI | 10.1128/AEM.02019-20 | |
Select in Zotero | zotero://select/library/items/IJ2YWFIK | |
Datum přidání | 23. 11. 2020 14:57:10 | |
Upraveno | 5. 3. 2021 10:55:55 |
Autor | A. Sharma | |
---|---|---|
Autor | B. Preece | |
Autor | H. Swann | |
Autor | X. Fan | |
Autor | R. J. McKenney | |
Autor | K. M. Ori-McKenney | |
Autor | S. Saffarian | |
Autor | M. D. Vershinin | |
Typ | Článek v časopise | |
Datum | 2020-10-14 | |
Abstrakt | SARS-CoV-2 is a novel coronavirus which has caused the COVID-19 pandemic. Other known coronaviruses show a strong pattern of seasonality, with the infection cases in humans being more prominent in winter. Although several plausible origins of such seasonal variability have been proposed, its mechanism is unclear. SARS-CoV-2 is transmitted via airborne droplets ejected from the upper respiratory tract of the infected individuals. It has been reported that SARS-CoV-2 can remain infectious for hours on surfaces. As such, the stability of viral particles both in liquid droplets as well as dried on surfaces is essential for infectivity. Here we have used atomic force microscopy to examine the structural stability of individual SARS-CoV-2 virus like particles at different temperatures. We demonstrate that even a mild temperature increase, commensurate with what is common for summer warming, leads to dramatic disruption of viral structural stability, especially when the heat is applied in the dry state. This is consistent with other existing non-mechanistic studies of viral infectivity, provides a single particle perspective on viral seasonality, and strengthens the case for a resurgence of COVID-19 in winter.</p><h3>Statement of Scientific Significance</h3> <p>The economic and public health impact of the COVID-19 pandemic are very significant. However scientific information needed to underpin policy decisions are limited partly due to novelty of the SARS-CoV-2 pathogen. There is therefore an urgent need for mechanistic studies of both COVID-19 disease and the SARS-CoV-2 virus. We show that individual virus particles suffer structural destabilization at relatively mild but elevated temperatures. Our nanoscale results are consistent with recent observations at larger scales. Our work strengthens the case for COVID-19 resurgence in winter. | |
Přístup | 16. 12. 2020 11:04:23 | |
Publikace | bioRxiv | |
Jazyk | en | |
Rozsah | 2020.10.12.336818 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author's permission. | |
Extra | Publisher: Cold Spring Harbor Laboratory Section: New Results | |
Katalog knihovny | www.biorxiv.org | |
DOI | 10.1101/2020.10.12.336818 | |
Select in Zotero | zotero://select/library/items/EIGFYGDD | |
Datum přidání | 16. 12. 2020 11:04:23 | |
Upraveno | 5. 3. 2021 10:56:00 |
Autor | Shane Riddell | |
---|---|---|
Autor | Sarah Goldie | |
Autor | Andrew Hill | |
Autor | Debbie Eagles | |
Autor | Trevor W. Drew | |
Typ | Článek v časopise | |
Datum | 2020-10-07 | |
Abstrakt | The rate at which COVID-19 has spread throughout the globe has been alarming. While the role of fomite transmission is not yet fully understood, precise data on the environmental stability of SARS-CoV-2 is required to determine the risks of fomite transmission from contaminated surfaces. | |
Přístup | 14. 10. 2020 11:44:30 | |
Publikace | Virology Journal | |
Jazyk | en | |
Ročník | 17 | |
Číslo | 1 | |
Rozsah | 145 | |
ISSN | 1743-422X | |
Katalog knihovny | Springer Link | |
Zkrácený název časopisu | Virol J | |
DOI | 10.1186/s12985-020-01418-7 | |
Select in Zotero | zotero://select/library/items/QELGBX8K | |
Datum přidání | 14. 10. 2020 11:44:30 | |
Upraveno | 5. 3. 2021 10:56:06 |
Autor | Narges Nazari Harmooshi | |
---|---|---|
Autor | Shirbandi Kiarash | |
Autor | Rahim Fakher | |
Typ | Článek v časopise | |
Datum | 2020-10 | |
Abstrakt | The new coronavirus, called 2019-nCoV, is a new type of virus that was first identified in Wuhan, China, in December 2019. Environmental conditions necessary for survival and spread of 2019-nCoV are somewhat transparent but unlike animal coronaviruses. We are poorly aware of their survival in environment and precise factors of their transmission. Countries located in east and west of globe did not have a significant impact on prevalence of disease among communities, and on the other hand, north and south have provided a model for relative prediction of disease outbreaks. The 2019-nCoV can survive for up to 9 days at 25 °C, and if this temperature rises to 30 °C, its lifespan will be shorter. The 2019-nCoV is sensitive to humidity, and lifespan of viruses in 50% humidity is longer than that of 30%. Also, temperature and humidity are important factors influencing the COVID-19 mortality rate and may facilitate 2019-nCoV transmission. Thus, considering the available and recent evidence, it seems that low temperatures, as well as dry and unventilated air, may affect stability and transmissibility of 2019-nCoV. | |
Publikace | Environmental Science and Pollution Research International | |
Jazyk | English | |
Ročník | 27 | |
Číslo | 29 | |
Rozsah | 36027-36036 | |
Archiv | ProQuest Central | |
Místo v archivu | 2438348165 | |
Extra | Place: Heidelberg Publisher: Springer Nature B.V. | |
ISSN | 0944-1344 | |
DOI | 10.1007/s11356-020-09733-w | |
Select in Zotero | zotero://select/library/items/CTUDGVAD | |
Datum přidání | 14. 10. 2020 10:37:15 | |
Upraveno | 5. 3. 2021 10:56:19 |
Copyright - © Springer-Verlag GmbH Germany, part of Springer Nature 2020.
Poslední aktualizace - 2020-08-30
SubjectsTermNotLitGenreText - Coronaviridae
Autor | Louisa F. Ludwig-Begall | |
---|---|---|
Autor | Constance Wielick | |
Autor | Lorène Dams | |
Autor | Hans Nauwynck | |
Autor | Pierre-Francois Demeuldre | |
Autor | Aurore Napp | |
Autor | Jan Laperre | |
Autor | Eric Haubruge | |
Autor | Etienne Thiry | |
Typ | Článek v časopise | |
Datum | 2020-09-01 | |
Abstrakt | Background In the context of the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, the supply of personal protective equipment remains under severe strain. To address this issue, re-use of surgical face masks and filtering facepiece respirators has been recommended; prior decontamination is paramount to their re-use. Aim We aim to provide information on the effects of three decontamination procedures on porcine respiratory coronavirus (PRCV)-contaminated masks and respirators, presenting a stable model for infectious coronavirus decontamination of these typically single-use-only products. Methods Surgical masks and filtering facepiece respirator coupons and straps were inoculated with infectious PRCV and submitted to three decontamination treatments, UV irradiation, vaporised H2O2, and dry heat treatment. Viruses were recovered from sample materials and viral titres were measured in swine testicle cells. Findings UV irradiation, vaporised H2O2 and dry heat reduced infectious PRCV by more than three orders of magnitude on mask and respirator coupons and rendered it undetectable in all decontamination assays. Conclusion This is the first description of stable disinfection of face masks and filtering facepiece respirators contaminated with an infectious SARS-CoV-2 surrogate using UV irradiation, vaporised H2O2 and dry heat treatment. The three methods permit demonstration of a loss of infectivity by more than three orders of magnitude of an infectious coronavirus in line with the FDA policy regarding face masks and respirators. It presents advantages of uncomplicated manipulation and utilisation in a BSL2 facility, therefore being easily adaptable to other respirator and mask types. | |
Publikace | Journal of Hospital Infection | |
ISSN | 0195-6701 | |
Zkrácený název časopisu | Journal of Hospital Infection | |
DOI | 10.1016/j.jhin.2020.08.025 | |
Select in Zotero | zotero://select/library/items/UA27P42N | |
Datum přidání | 10. 9. 2020 15:26:30 | |
Upraveno | 5. 3. 2021 10:56:28 |
Autor | M. J. Pascoe | |
---|---|---|
Autor | A. Robertson | |
Autor | A. Crayford | |
Autor | E. Durand | |
Autor | J. Steer | |
Autor | A. Castelli | |
Autor | R. Wesgate | |
Autor | S. L. Evans | |
Autor | A. Porch | |
Autor | J-Y. Maillard | |
Typ | Článek v časopise | |
Datum | 2020-09-01 | |
Abstrakt | Background In the wake of the SARS-CoV-2 pandemic and unprecedented global demand, clinicians are struggling to source adequate access to personal protective equipment. Respirators can be in short supply, though are necessary to protect workers from SARS-CoV-2 exposure. Rapid decontamination and reuse of respirators may provide relief for the strained procurement situation. Method In this study, we investigated the suitability of 70°C dry heat and microwave-generated steam (MGS) for reprocessing of FFP2/N95-type respirators, and Type-II surgical face masks. Staphylococcus aureus was used as a surrogate as it is less susceptible than enveloped viruses to chemical and physical processes. Results We observed >4 log10 reductions in the viability of dry S. aureus treated by dry heat for 90 min at 70°C and >6 log10 reductions by MGS for 90 s. After 3 reprocessing cycles, neither process was found to negatively impact the bacterial or NaCl filtration efficiency of the respirators that were tested. However, MGS was incompatible with Type-II surgical masks tested, as we confirmed that bacterial filtration capacity was completely lost following reprocessing. MGS was observed to be incompatible with some respirator types due to arcing observed around some types of metal nose clips and by loss of adhesion of clips to the mask. Conclusion Considering the advantages and disadvantages of each approach, we propose a reprocessing personal protective equipment/face mask workflow for use in medical areas. | |
Přístup | 15. 1. 2021 15:17:16 | |
Publikace | Journal of Hospital Infection | |
Jazyk | en | |
Ročník | 106 | |
Číslo | 1 | |
Rozsah | 10-19 | |
ISSN | 0195-6701 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Hospital Infection | |
DOI | 10.1016/j.jhin.2020.07.008 | |
Select in Zotero | zotero://select/library/items/VLI8ND97 | |
Datum přidání | 15. 1. 2021 15:17:16 | |
Upraveno | 5. 3. 2021 10:56:25 |
Autor | Jennifer Biryukov | |
---|---|---|
Autor | Jeremy A. Boydston | |
Autor | Rebecca A. Dunning | |
Autor | John J. Yeager | |
Autor | Stewart Wood | |
Autor | Amy L. Reese | |
Autor | Allison Ferris | |
Autor | David Miller | |
Autor | Wade Weaver | |
Autor | Nathalie E. Zeitouni | |
Autor | Aaron Phillips | |
Autor | Denise Freeburger | |
Autor | Idris Hooper | |
Autor | Shanna Ratnesar-Shumate | |
Autor | Jason Yolitz | |
Autor | Melissa Krause | |
Autor | Gregory Williams | |
Autor | David G. Dawson | |
Autor | Artemas Herzog | |
Autor | Paul Dabisch | |
Autor | Victoria Wahl | |
Autor | Michael C. Hevey | |
Autor | Louis A. Altamura | |
Typ | Článek v časopise | |
Datum | 2020-08-26 | |
Abstrakt | Coronavirus disease 2019 (COVID-19) was first identified in China in late 2019 and is caused by newly identified severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Previous studies had reported the stability of SARS-CoV-2 in cell culture media and deposited onto surfaces under a limited set of environmental conditions. Here, we broadly investigated the effects of relative humidity, temperature, and droplet size on the stability of SARS-CoV-2 in a simulated clinically relevant matrix dried on nonporous surfaces. The results show that SARS-CoV-2 decayed more rapidly when either humidity or temperature was increased but that droplet volume (1 to 50 μl) and surface type (stainless steel, plastic, or nitrile glove) did not significantly impact decay rate. At room temperature (24°C), virus half-life ranged from 6.3 to 18.6 h depending on the relative humidity but was reduced to 1.0 to 8.9 h when the temperature was increased to 35°C. These findings suggest that a potential for fomite transmission may persist for hours to days in indoor environments and have implications for assessment of the risk posed by surface contamination in indoor environments. IMPORTANCE Mitigating the transmission of SARS-CoV-2 in clinical settings and public spaces is critically important to reduce the number of COVID-19 cases while effective vaccines and therapeutics are under development. SARS-CoV-2 transmission is thought to primarily occur through direct person-to-person transfer of infectious respiratory droplets or through aerosol-generating medical procedures. However, contact with contaminated surfaces may also play a significant role. In this context, understanding the factors contributing to SARS-CoV-2 persistence on surfaces will enable a more accurate estimation of the risk of contact transmission and inform mitigation strategies. To this end, we have developed a simple mathematical model that can be used to estimate virus decay on nonporous surfaces under a range of conditions and which may be utilized operationally to identify indoor environments in which the virus is most persistent. | |
Přístup | 4. 3. 2021 12:03:10 | |
Publikace | mSphere | |
Jazyk | en | |
Ročník | 5 | |
Číslo | 4 | |
Práva | Copyright © 2020 Biryukov et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. | |
Extra | Publisher: American Society for Microbiology Journals Section: Observation PMID: 32611701 | |
ISSN | 2379-5042 | |
Katalog knihovny | msphere.asm.org | |
DOI | 10.1128/mSphere.00441-20 | |
Select in Zotero | zotero://select/library/items/DAZKSF6K | |
Datum přidání | 4. 3. 2021 12:02:51 | |
Upraveno | 5. 3. 2021 10:56:45 |
Autor | Shira Gertsman | |
---|---|---|
Autor | Anirudh Agarwal | |
Autor | Katharine O’Hearn | |
Autor | Richard Webster | |
Autor | Anne Tsampalieros | |
Autor | Nick Barrowman | |
Autor | Margaret Sampson | |
Autor | Lindsey Sikora | |
Autor | Emiliyan Staykov | |
Autor | Rhiannon Ng | |
Autor | Jess Gibson | |
Autor | Tri Dinh | |
Autor | Kwame Agyei | |
Autor | Graham Chamberlain | |
Autor | James Dayre McNally | |
Typ | Článek v časopise | |
Datum | 2020-08-22 | |
Abstrakt | Background In pandemics such as COVID-19, shortages of personal protective equipment are common. One solution may be to decontaminate equipment such as facemasks for reuse. Aim To collect and synthesize existing information on decontamination of N95 filtering facepiece respirators (FFRs) using microwave and heat-based treatments, with special attention to impact on mask function (aerosol penetration, airflow resistance), fit, and physical traits. Methods A systematic review (PROSPERO CRD42020177036) of literature available from Medline, Embase, Global Health, and other sources was conducted. Records were screened independently by two reviewers, and data was extracted from studies that reported on effects of microwave- or heat-based decontamination on N95 FFR performance, fit, physical traits, and/or reductions in microbial load. Findings Thirteen studies were included that used dry/moist microwave irradiation, heat, or autoclaving. All treatment types reduced pathogen load by a log10 reduction factor of at least three when applied for sufficient duration (>30s microwave, >60 min dry heat), with most studies assessing viral pathogens. Mask function (aerosol penetration <5% and airflow resistance <25mmH2O) was preserved after all treatments except autoclaving. Fit was maintained for most N95 models, though all treatment types caused observable physical damage to at least one model. Conclusions Microwave irradiation and heat may be safe and effective viral decontamination options for N95 FFR reuse during critical shortages. The evidence does not support autoclaving or high-heat (>90oC) approaches. Physical degradation may be an issue for certain mask models, and more real-world evidence on fit is needed. | |
Přístup | 11. 9. 2020 13:35:26 | |
Publikace | Journal of Hospital Infection | |
Krátký název | Microwave- and Heat-Based Decontamination of N95 Filtering Facepiece Respirators | |
Jazyk | en | |
ISSN | 0195-6701 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Hospital Infection | |
DOI | 10.1016/j.jhin.2020.08.016 | |
Select in Zotero | zotero://select/library/items/TU22KGP9 | |
Datum přidání | 10. 9. 2020 15:29:59 | |
Upraveno | 5. 3. 2021 10:56:53 |
Autor | Denis Corpet | |
---|---|---|
Typ | Článek v časopise | |
Datum | 2020-08-20 | |
Abstrakt | The Covid-19 coronavirus, SARS-CoV-2, is inactivated much faster on paper (3h) than on plastic (7d). By classifying materials according to virus stability on their surface, the following list is obtained (from long to short stability): polypropylene (mask), plastic, glass, stainless steel, pig skin, cardboard, banknote, cotton, wood, paper, tissue, copper. These observations and other studies suggest that SARS-CoV-2 may be inactivated by dryness on water absorbent porous materials but sheltered by long-persisting micro-droplets of water on waterproof surfaces. If such physical phenomenons were confirmed by direct evidence, the persistence of the virus on any surface could be predicted, and new porous objects could be designed to eliminate the virus faster. | |
Publikace | Preprints | |
Jazyk | English | |
Archiv | ProQuest Central | |
Místo v archivu | 2435631056 | |
Extra | Place: Basel Publisher: MDPI AG | |
DOI | 10.20944/preprints202008.0426.v1 | |
Select in Zotero | zotero://select/library/items/JLGTBU9H | |
Datum přidání | 21. 8. 2020 7:45:36 | |
Upraveno | 5. 3. 2021 10:56:59 |
Copyright - © 2020. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Poslední aktualizace - 2020-08-21
Autor | Jennifer L. Welch | |
---|---|---|
Autor | Jinhua Xiang | |
Autor | Samantha R. Mackin | |
Autor | Stanley Perlman | |
Autor | Peter Thorne | |
Autor | Patrick O’Shaughnessy | |
Autor | Brian Strzelecki | |
Autor | Patrick Aubin | |
Autor | Monica Ortiz-Hernandez | |
Autor | Jack T. Stapleton | |
Typ | Článek v časopise | |
Datum | 2020-08-12 | |
Abstrakt | Background: Personal protective equipment (PPE) is a critical need during the coronavirus disease 2019 (COVID-19) pandemic. Alternative sources of surgical masks, including 3-dimensionally (3D) printed approaches that may be reused, are urgently needed to prevent PPE shortages. Few data exist identifying decontamination strategies to inactivate viral pathogens and retain 3D-printing material integrity. Objective: To test viral disinfection methods on 3D-printing materials. Methods: The viricidal activity of common disinfectants (10% bleach, quaternary ammonium sanitizer, 3% hydrogen peroxide, or 70% isopropanol and exposure to heat (50°C, and 70°C) were tested on four 3D-printed materials used in the healthcare setting, including a surgical mask design developed by the Veterans’ Health Administration. Inactivation was assessed for several clinically relevant RNA and DNA pathogenic viruses, including severe acute respiratory coronavirus virus 2 (SARS-CoV-2) and human immunodeficiency virus 1 (HIV-1). Results: SARS-CoV-2 and all viruses tested were completely inactivated by a single application of bleach, ammonium quaternary compounds, or hydrogen peroxide. Similarly, exposure to dry heat (70°C) for 30 minutes completely inactivated all viruses tested. In contrast, 70% isopropanol reduced viral titers significantly less well following a single application. Inactivation did not interfere with material integrity of the 3D-printed materials. Conclusions: Several standard decontamination approaches effectively disinfected 3D-printed materials. These approaches were effective in the inactivation SARS-CoV-2, its surrogates, and other clinically relevant viral pathogens. The decontamination of 3D-printed surgical mask materials may be useful during crisis situations in which surgical mask supplies are limited. | |
Přístup | 14. 9. 2020 10:20:04 | |
Publikace | Infection Control and Hospital Epidemiology | |
Jazyk | en | |
Ročník | 42 | |
Číslo | 3 | |
Rozsah | 1-8 | |
Archiv | Coronavirus Research Database | |
Místo v archivu | 2439112105 | |
Extra | PMID: 32783787 PMCID: PMC7463154 | |
ISSN | 0899-823X | |
Katalog knihovny | PubMed Central | |
Zkrácený název časopisu | Infect Control Hosp Epidemiol | |
DOI | 10.1017/ice.2020.417 | |
Select in Zotero | zotero://select/library/items/N4SR2N5M | |
Datum přidání | 21. 8. 2020 9:29:41 | |
Upraveno | 26. 3. 2021 13:23:05 |
Copyright - © 2020. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Poslední aktualizace - 2020-09-10
Autor | Yu Wu | |
---|---|---|
Autor | Wenzhan Jing | |
Autor | Jue Liu | |
Autor | Qiuyue Ma | |
Autor | Jie Yuan | |
Autor | Yaping Wang | |
Autor | Min Du | |
Autor | Min Liu | |
Typ | Článek v časopise | |
Datum | 2020-08-10 | |
Abstrakt | The coronavirus disease 2019 (COVID-19) pandemic is the defining global health crisis of our time and the greatest challenge facing the world. Meteorological parameters are reportedly crucial factors affecting respiratory infectious disease epidemics; however, the effect of meteorological parameters on COVID-19 remains controversial. This study investigated the effects of temperature and relative humidity on daily new cases and daily new deaths of COVID-19, which has useful implications for policymakers and the public. Daily data on meteorological conditions, new cases and new deaths of COVID-19 were collected for 166 countries (excluding China) as of March 27, 2020. Log-linear generalized additive model was used to analyze the effects of temperature and relative humidity on daily new cases and daily new deaths of COVID-19, with potential confounders controlled for, including wind speed, median age of the national population, Global Health Security Index, Human Development Index and population density. Our findings revealed that temperature and relative humidity were both negatively related to daily new cases and deaths. A 1 °C increase in temperature was associated with a 3.08% (95% CI: 1.53%, 4.63%) reduction in daily new cases and a 1.19% (95% CI: 0.44%, 1.95%) reduction in daily new deaths, whereas a 1% increase in relative humidity was associated with a 0.85% (95% CI: 0.51%, 1.19%) reduction in daily new cases and a 0.51% (95% CI: 0.34%, 0.67%) reduction in daily new deaths. The results remained robust when different lag structures and the sensitivity analysis were used. These findings provide preliminary evidence that the COVID-19 pandemic may be partially suppressed with temperature and humidity increases. However, active measures must be taken to control the source of infection, block transmission and prevent further spread of COVID-19. | |
Přístup | 2. 7. 2020 15:10:41 | |
Publikace | Science of The Total Environment | |
Jazyk | en | |
Ročník | 729 | |
ISSN | 0048-9697 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Science of The Total Environment | |
DOI | 10.1016/j.scitotenv.2020.139051 | |
Select in Zotero | zotero://select/library/items/2XZCCD42 | |
Datum přidání | 20. 5. 2020 14:48:27 | |
Upraveno | 5. 3. 2021 10:57:11 |
Autor | Te Faye Yap | |
---|---|---|
Autor | Zhen Liu | |
Autor | Rachel A. Shveda | |
Autor | Daniel J. Preston | |
Typ | Článek v časopise | |
Datum | 2020-08-10 | |
Abstrakt | The COVID-19 pandemic has stressed healthcare systems and supply lines, forcing medical doctors to risk infection by decontaminating and reusing single-use personal protective equipment. The uncertain future of the pandemic is compounded by limited data on the ability of the responsible virus, SARS-CoV-2, to survive across various climates, preventing epidemiologists from accurately modeling its spread. However, a detailed thermodynamic analysis of experimental data on the inactivation of SARS-CoV-2 and related coronaviruses can enable a fundamental understanding of their thermal degradation that will help model the COVID-19 pandemic and mitigate future outbreaks. This work introduces a thermodynamic model that synthesizes existing data into an analytical framework built on first principles, including the rate law for a first-order reaction and the Arrhenius equation, to accurately predict the temperature-dependent inactivation of coronaviruses. The model provides much-needed thermal decontamination guidelines for personal protective equipment, including masks. For example, at 70 °C, a 3-log (99.9%) reduction in virus concentration can be achieved, on average, in 3 min (under the same conditions, a more conservative decontamination time of 39 min represents the upper limit of a 95% interval) and can be performed in most home ovens without reducing the efficacy of typical N95 masks as shown in recent experimental reports. This model will also allow for epidemiologists to incorporate the lifetime of SARS-CoV-2 as a continuous function of environmental temperature into models forecasting the spread of the pandemic across different climates and seasons. | |
Přístup | 3. 5. 2021 13:34:21 | |
Publikace | Applied Physics Letters | |
Ročník | 117 | |
Číslo | 6 | |
Rozsah | 060601 | |
Extra | Publisher: American Institute of Physics | |
ISSN | 0003-6951 | |
Katalog knihovny | aip.scitation.org (Atypon) | |
Zkrácený název časopisu | Appl. Phys. Lett. | |
DOI | 10.1063/5.0020782 | |
Select in Zotero | zotero://select/library/items/F6CTTQ8M | |
Datum přidání | 3. 5. 2021 13:34:21 | |
Upraveno | 4. 5. 2021 15:51:46 |
Autor | Ze-gang Wu | |
---|---|---|
Autor | Hong-yun Zheng | |
Autor | Jian Gu | |
Autor | Feng Li | |
Autor | Rui-long Lv | |
Autor | Ya-yun Deng | |
Autor | Wan-zhou Xu | |
Autor | Yong-qing Tong | |
Typ | Článek v časopise | |
Datum | 2020-08-07 | |
Abstrakt | The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20–25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65°C for 10 min may be sufficient to ensure safe, reliable testing. | |
Přístup | 21. 8. 2020 8:49:04 | |
Publikace | Current Medical Science | |
Jazyk | en | |
ISSN | 2523-899X | |
Katalog knihovny | Springer Link | |
Zkrácený název časopisu | CURR MED SCI | |
DOI | 10.1007/s11596-020-2224-y | |
Select in Zotero | zotero://select/library/items/UVMIEKPN | |
Datum přidání | 21. 8. 2020 8:49:04 | |
Upraveno | 5. 3. 2021 10:57:15 |
Autor | Rajib Banerjee | |
---|---|---|
Autor | Pulakesh Roy | |
Autor | Surajeet Das | |
Autor | Manash K. Paul | |
Typ | Článek v časopise | |
Datum | 2020-07-28 | |
Abstrakt | The pandemic coronavirus disease 2019 (COVID-19) has taken a heavy toll on human life and has upended the medical system in many countries. The disease has created a system wide worsening shortage of N95, medical masks, and other personal protective equipment (PPE) that is regularly used by healthcare personnel and emergency service providers for their protection. Considering the number of infected patients and the stressed supplies of PPE reuse of PPE can serve as an efficient contingency plan. Multiple studies have investigated the effect of different decontamination methods. We chose the most user-friendly, easily scalable viral decontamination methods, including ultraviolet (UV) irradiation and heat treatment. In this paper, we investigated a unique approach to reuse the mask by creating a hybrid model that efficiently sanitizes the infected mask. The advantages of the proposed hybrid model as compared to the respective single arms is its decontamination efficacy, operational speed, as well as the number of reuse cycles as verified by mathematical analysis and simulation. This model is mainly intended for medical PPE but can also be used for other domestic and personal sanitization during the COVID-19 pandemic. As per the situation, the hybrid system can be used as standalone systems also. This sanitization process is not only limited to the elimination of SARS-CoV-2 but can be extended to any other infectious agents. Thus, our results indicate that the proposed hybrid system is more effective, meets disinfection criterion and time saving for the reuse of respirators and PPE. | |
Přístup | 21. 8. 2020 8:40:46 | |
Publikace | American Journal of Infection Control | |
Jazyk | en | |
ISSN | 0196-6553 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | American Journal of Infection Control | |
DOI | 10.1016/j.ajic.2020.07.022 | |
Select in Zotero | zotero://select/library/items/Z5JXL7GC | |
Datum přidání | 21. 8. 2020 8:40:46 | |
Upraveno | 5. 3. 2021 10:57:20 |
Autor | Yuri D. Lensky | |
---|---|---|
Autor | Edward A. Mazenc | |
Autor | Daniel Ranard | |
Autor | Matthew Vilim | |
Autor | Manu Prakash | |
Autor | Bill Brooks | |
Autor | Amanda Bradley | |
Autor | Alain Engelschenschilt | |
Autor | Jason Plutz | |
Autor | Todd Zellmer | |
Typ | Článek v časopise | |
Datum | 2020-07-24 | |
Abstrakt | <h3>Abstract</h3> <p>We propose a dry heat method for decontaminating N95 masks of SARS-CoV-2, designed around placing them in resealable plastic bags, packed in large cardboard boxes installed at the rear end of commercial laundry dryers. Our protocol rests on data collected in collaboration with Alliance Laundry Systems (ALS) and the CDC/NIOSH laboratories, under the “NPPTL Respirator Assessments to Support the COVID-19 Response” initiative. We test the two most widely available ALS tumbler models, the UTF75N and UT075N, and show that if our procedure is carefully followed, the masks will be subject to suitably high and stable temperatures for decontamination; in particular the masks will be heated to at least 80 °C for at least 65 min. For the mask models 3M 1860, 3M 8511, and Halyard 62126, we establish that they pass quantitative fit tests and retain sufficient filtration performance after three cycles of our decontamination procedure. All masks used in this study were new and uncontaminated: the evidence for the levels of biological inactivation of SARS-CoV-2 is provided by [1]. While the protocol outlined here is currently specific to certain tested dryer models, this equipment is widely available, with machines estimated to be within 15 minutes of most US hospitals. Models from other manufacturers may also be appropriate for this decontamination method, though we stress the need for explicit testing on alternative models before use.</p> | |
Přístup | 12. 4. 2021 15:10:12 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.07.22.20160283 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NoDerivs 4.0 International), CC BY-ND 4.0, as described at http://creativecommons.org/licenses/by-nd/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
ISSN | 2016-0283 | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.07.22.20160283 | |
Select in Zotero | zotero://select/library/items/FUNH7R5Y | |
Datum přidání | 12. 4. 2021 15:10:12 | |
Upraveno | 9. 7. 2021 9:10:50 |
Autor | Martin Hessling | |
---|---|---|
Autor | Katharina Hoenes | |
Autor | Christian Lingenfelder | |
Typ | Článek v časopise | |
Datum | 2020-07-13 | |
Abstrakt | Hintergrund: Um Infektionen mit dem neuen Coronavirus SARS-CoV-2 zu vermeiden, setzen sowohl Beschäftigte im Gesundheitswesen als auch große Teile der Bevölkerung derzeit persönliche Schutzausrüstung, wie Gesichtsmasken, ein. Diese Ausrüstung muss vor der Wiederverwendung möglichst schonend sterilisiert werden. Eine Möglichkeit dafür ist die thermische Inaktivierung. Professionelle Autoklaven mit ihren hohen Temperaturen sind jedoch oft nicht verfügbar oder geeignet. Bei Coronaviren kann die Inaktivierung aber auch bei niedrigeren Temperaturen erfolgen, wenn die Inaktvierungsdauer lang genug ist. Diese notwendige Dauer soll hier bestimmt werden. Material und Methoden: Daten aus publizierten thermischen Inaktivierungsstudien an Coronaviren werden herangezogen, um mit Hilfe von Arrhenius-Modellen für jedes Coronavirus die Temperaturabhängigkeit der Ratenkonstante k(T) zu bestimmen. Ergebnisse: Die ermittelten Daten weisen große Variationen auf, die möglicherweise auch auf unterschiedliche Probeneigenschaften zurückzuführen sind. Coronaviren in Proben mit hohem Proteingehalt oder Proben an trockener Luft scheinen manchmal schwerer zu inaktivieren sein. Abgesehen davon scheint das Arrhenius-Modell die thermischen Inaktivierungseigenschaften gut zu beschreiben, wobei sich SARS-CoV und SARS-CoV-2 durch ein gemeinsames Modell darstellen lassen. Darüber hinaus deuten die verfügbaren Daten darauf hin, dass alle Proben mathematisch durch ein Worst-Case-Modell berücksichtigt werden können, das auch potentiell kritische Proben miteinschließt.Schlussfolgerung: Coronaviren lassen sich bereits durch relativ niedrige Temperaturen inaktivieren. Für Standardproben sind z.B. bei 60°C, 80°C und 100°C Anwendungsdauern von ca. 32,5, 3,7 und 0,5 min notwendig, um eine Reduktion von 5 Logstufen zu erreichen. Für eine sicherere Inaktivierung liefert das Worst-Case-Modell für die genannten Temperaturen deutlich längere Anwendungszeiten von 490, 55 und 8 min. | |
Publikace | GMS Hygiene and Infection Control | |
Jazyk | engl | |
Ročník | 15 | |
Rozsah | Doc16 | |
Práva | This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. | |
Extra | Publisher: German Medical Science GMS Publishing House | |
ISSN | 2196-5226 | |
Katalog knihovny | GMS German Medical Science | |
Zkrácený název časopisu | GMS Hyg Infect Control | |
DOI | 10.3205/dgkh000351 | |
Select in Zotero | zotero://select/library/items/3MT68B45 | |
Datum přidání | 21. 8. 2020 7:49:59 | |
Upraveno | 21. 8. 2020 7:49:59 |
Autor | Kwok-Hung Chan | |
---|---|---|
Autor | Siddharth Sridhar | |
Autor | Ricky Ruiqi Zhang | |
Autor | Hin Chu | |
Autor | Agnes Yim-Fong Fung | |
Autor | Gabriella Chan | |
Autor | Jasper Fuk-Woo Chan | |
Autor | Kelvin Kai-Wang To | |
Autor | Ivan Fan-Ngai Hung | |
Autor | Vincent Chi-Chung Cheng | |
Autor | Kwok-Yung Yuen | |
Typ | Článek v časopise | |
Datum | 2020-07-09 | |
Abstrakt | Background In late 2019, a novel human coronavirus, SARS-CoV-2, emerged in Wuhan, China. This virus has caused a global pandemic involving more than 200 countries. SARS-CoV-2 is highly adapted to humans and readily transmits from person-to-person. Aim The aim of this study was to investigate the infectivity of SARS-CoV-2 under various environmental factors, disinfectants and different pH conditions. The efficacy of a variety of laboratory virus inactivation methods and home disinfectants against SARS-CoV-2 were investigated. Methods The residual virus in dried form or in solution was titrated on Vero E6 cell line at day 0, 1, 3, 5, and 7 after incubation at different temperatures. The viability of virus was determined after treatment with different disinfectants and pH solutions at room temperature (20∼25oC). Findings SARS-CoV-2 was able to retain viability for 3-5 days in dried form or 7 days in solution at room temperature. SARS-CoV-2 could be detected under a wide range of pH conditions from pH4 to pH11 for several days and 1 to 2 days in stool at room temperature but lost 5 logs of infectivity. A variety of commonly used disinfectants and laboratory inactivation procedures were found to reduce viral viability effectively. Conclusion This study demonstrates the stability of SARS-CoV-2 on environmental surfaces and raises the possibility of faecal-oral transmission. Commonly used fixatives, nucleic acid extraction methods and heat inactivation were found to significantly reduce viral infectivity that could ensure hospital and laboratory safety during the COVID-19 pandemic. | |
Přístup | 28. 7. 2020 9:00:29 | |
Publikace | Journal of Hospital Infection | |
Jazyk | en | |
ISSN | 0195-6701 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Hospital Infection | |
DOI | 10.1016/j.jhin.2020.07.009 | |
Select in Zotero | zotero://select/library/items/M36S6YUU | |
Datum přidání | 27. 7. 2020 13:14:14 | |
Upraveno | 5. 3. 2021 10:34:58 |
Autor | John P. Abraham | |
---|---|---|
Autor | Brian D. Plourde | |
Autor | Lijing Cheng | |
Typ | Článek v časopise | |
Datum | 2020-07-02 | |
Abstrakt | The current coronavirus pandemic has reached global proportions and requires unparalleled collective and individual efforts to slow its spread. One critically important issue is the proper sterilization of physical objects that have been contaminated by the virus. Here, we review the currently existing literature on thermal inactivation of coronavirus (SARS-CoV-2) and present preliminary guideless on temperatures and exposure durations required to sterilize. We also compare these temperatures/exposure durations with potential household appliances that may be thought capable of performing sterilization. | |
Přístup | 2. 7. 2020 15:34:57 | |
Publikace | Reviews in Medical Virology | |
Jazyk | en | |
Ročník | n/a | |
Číslo | n/a | |
Rozsah | e2115 | |
Práva | © 2020 John Wiley & Sons, Ltd | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/rmv.2115 | |
ISSN | 1099-1654 | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1002/rmv.2115 | |
Select in Zotero | zotero://select/library/items/MQZ5FGRI | |
Datum přidání | 2. 7. 2020 15:34:57 | |
Upraveno | 5. 3. 2021 10:57:33 |
Autor | Boris Pastorino | |
---|---|---|
Autor | Franck Touret | |
Autor | Magali Gilles | |
Autor | Xavier de Lamballerie | |
Autor | Remi N. Charrel | |
Typ | Článek v časopise | |
Datum | 2020-07 | |
Abstrakt | Standard precautions to minimize the risk of SARS-CoV-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort of inactivation to reduce or abolish infectivity. We evaluated three heat inactivation protocols (56 °C-30 min, 60 °C-60 min and 92 °C-15 min) on SARS-CoV-2 using (i) infected cell culture supernatant, (ii) virus-spiked human sera (iii) and nasopharyngeal samples according to the recommendations of the European norm NF EN 14476-A2. Regardless of the protocol and the type of samples, a 4 Log10 TCID50 reduction was observed. However, samples containing viral loads > 6 Log10 TCID50 were still infectious after 56 °C-30 min and 60 °C-60 min, although infectivity was < 10 TCID50. The protocols 56 °C-30 min and 60 °C-60 min had little influence on the RNA copies detection, whereas 92 °C-15 min drastically reduced the limit of detection, which suggests that this protocol should be avoided for inactivation ahead of molecular diagnostics. Lastly, 56 °C-30 min treatment of serum specimens had a negligible influence on the results of IgG detection using a commercial ELISA test, whereas a drastic decrease in neutralizing titers was observed. | |
Přístup | 4. 3. 2021 12:02:19 | |
Publikace | Viruses | |
Krátký název | Heat Inactivation of Different Types of SARS-CoV-2 Samples | |
Jazyk | en | |
Ročník | 12 | |
Číslo | 7 | |
Rozsah | 735 | |
Práva | http://creativecommons.org/licenses/by/3.0/ | |
Extra | Number: 7 Publisher: Multidisciplinary Digital Publishing Institute | |
Katalog knihovny | www.mdpi.com | |
DOI | 10.3390/v12070735 | |
Select in Zotero | zotero://select/library/items/CGECHAWG | |
Datum přidání | 4. 3. 2021 12:02:19 | |
Upraveno | 13. 4. 2021 14:54:31 |
Autor | Nicole Rockey | |
---|---|---|
Autor | Peter J. Arts | |
Autor | Lucinda Li | |
Autor | Katherine R. Harrison | |
Autor | Kathryn Langenfeld | |
Autor | William J. Fitzsimmons | |
Autor | Adam S. Lauring | |
Autor | Nancy G. Love | |
Autor | Keith S. Kaye | |
Autor | Lutgarde Raskin | |
Autor | William W. Roberts | |
Autor | Bridget Hegarty | |
Autor | Krista R. Wigginton | |
Typ | Článek v časopise | |
Datum | 2020-06-23 | |
Abstrakt | Supply shortages of N95 respirators during the coronavirus disease 2019 (COVID-19) pandemic have motivated institutions to develop feasible and effective N95 respirator reuse strategies. In particular, heat decontamination is a treatment method that scales well and can be implemented in settings with variable or limited resources. Prior studies using multiple inactivation methods, however, have often focused on a single virus under narrowly defined conditions, making it difficult to develop guiding principles for inactivating emerging or difficult-to-culture viruses. We systematically explored how temperature, humidity, and virus deposition solutions impact the inactivation of viruses deposited and dried on N95 respirator coupons. We exposed four virus surrogates across a range of structures and phylogenies, including two bacteriophages (MS2 and phi6), a mouse coronavirus (murine hepatitis virus, MHV), and a recombinant human influenza A virus subtype H3N2 (IAV), to heat treatment for 30 minutes in multiple deposition solutions across several temperatures and relative humidities (RH). We observed that elevated RH was essential for effective heat inactivation of all four viruses tested. For heat treatments between 72°C and 82°C, RH greater than 50% resulted in > 6-log10 inactivation of bacteriophages and RH greater than 25% resulted in > 3.5-log10 inactivation of MHV and IAV. Furthermore, deposition of viruses in host cell culture media greatly enhanced virus inactivation by heat and humidity compared to other deposition solutions such as phosphate buffered saline, phosphate buffered saline with bovine serum albumin, and human saliva. Past and future heat treatment methods or technologies must therefore explicitly account for deposition solutions as a factor that will strongly influence observed virus inactivation rates. Overall, our data set can inform the design and validation of effective heat-based decontamination strategies for N95 respirators and other porous surfaces, especially for emerging or low-titer viruses that may be of immediate public health concern such as SARS-CoV-2. | |
Přístup | 3. 7. 2020 8:00:56 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.06.22.20137448 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.06.22.20137448 | |
Select in Zotero | zotero://select/library/items/5K8BSSWL | |
Datum přidání | 3. 7. 2020 8:00:56 | |
Upraveno | 5. 3. 2021 10:57:45 |
Autor | Abbas Johar Jinia | |
---|---|---|
Autor | Noora Ba Sunbul | |
Autor | Christopher A. Meert | |
Autor | Cameron A. Miller | |
Autor | Shaun D. Clarke | |
Autor | Kimberlee J. Kearfott | |
Autor | Martha M. Matuszak | |
Autor | Sara A. Pozzi | |
Typ | Článek v časopise | |
Datum | 2020-06-16 | |
Abstrakt | The outbreak of the novel coronavirus disease, COVID-19 turned into a global pandemic in March 2020. During these unprecedented times, there is an increased demand in medical and personal protective equipment (PPE). Since the supplies may take a long time to meet the global demand, reusing PPEs will help health care workers in their response to the COVID-19 pandemic. To ensure the safety and well-being of the medical first responders, PPE needs to be sterilized before reuse. In this review, we examine various sterilization techniques that can be used to sterilize PPEs and point out its limitations. The objective is to provide a foundation of knowledge incorporating different sterilization techniques that allow hospitals and clinics to pick the most suitable technique for sterilization of a particular PPE. | |
Publikace | IEEE Access | |
Ročník | 8 | |
Rozsah | 111347-111354 | |
Extra | Conference Name: IEEE Access | |
ISSN | 2169-3536 | |
Katalog knihovny | IEEE Xplore | |
DOI | 10.1109/ACCESS.2020.3002886 | |
Select in Zotero | zotero://select/library/items/39X842HM | |
Datum přidání | 27. 7. 2020 13:43:51 | |
Upraveno | 21. 8. 2020 7:48:33 |
Autor | Louisa F. Ludwig-Begall | |
---|---|---|
Autor | Constance Wielick | |
Autor | Lorene Dams | |
Autor | Hans Nauwynck | |
Autor | Pierre-Francois Demeuldre | |
Autor | Aurore Napp | |
Autor | Jan Laperre | |
Autor | Eric Haubruge | |
Autor | Etienne Thiry | |
Typ | Článek v časopise | |
Datum | 2020-06-05 | |
Abstrakt | Background: In the context of the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, the supply of personal protective equipment remains under severe strain. To address this issue, re-use of surgical face masks and filtering facepiece respirators has been recommended; prior decontamination is paramount to their re-use. Aim: We aim to provide information on the effects of three decontamination procedures on porcine respiratory coronavirus (PRCV)-contaminated masks and respirators, presenting a stable model for infectious coronavirus decontamination of these typically single-use-only products. Methods: Surgical masks and filtering facepiece respirator coupons and straps were inoculated with infectious PRCV and submitted to three decontamination treatments, UV irradiation, H2O2 vaporisation, and dry heat treatment. Viruses were recovered from sample materials and viral titres were measured in swine testicle cells. Findings: UV irradiation, H2O2 vaporisation and dry heat reduced infectious PRCV by more than three orders of magnitude on mask and respirator coupons and rendered it undetectable in all decontamination assays. Conclusion: This is the first description of stable disinfection of face masks and filtering facepiece respirators contaminated with an infectious SARS-CoV-2 surrogate using UV irradiation, H2O2 vaporisation and dry heat treatment. The three methods permit demonstration of a loss of infectivity by more than three orders of magnitude of an infectious coronavirus in line with the FDA policy regarding face masks and respirators. It presents advantages of uncomplicated manipulation and utilisation in a BSL2 facility, therefore being easily adaptable to other respirator and mask types. | |
Přístup | 3. 7. 2020 8:03:09 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.06.02.20119834 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author's permission. | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.06.02.20119834 | |
Select in Zotero | zotero://select/library/items/NI9N8ATA | |
Datum přidání | 3. 7. 2020 8:03:09 | |
Upraveno | 5. 3. 2021 10:57:55 |
Autor | Cole Meisenhelder | |
---|---|---|
Autor | Loïc Anderegg | |
Autor | Andrew Preecha | |
Autor | Chiu Oan Ngooi | |
Autor | Lei Liao | |
Autor | Wang Xiao | |
Autor | Steven Chu | |
Autor | Yi Cui | |
Autor | John M. Doyle | |
Typ | Článek v časopise | |
Datum | 2020-06-02 | |
Abstrakt | <p>Current shortages of Filtering Facepiece Respirators (FFRs) have created a demand for effective methods for N95 decontamination and reuse. Before implementing any reuse strategy it is important to determine what effects the proposed method has on the physical functioning of the FFR. Here we investigate the effects of two potential methods for decontamination; dry heat at 95 °C, and autoclave treatments. We test both fit and filtration efficiency for each method. For the dry heat treatment we consider the 3M 1860, 3M 1870, and 3M8210+ models. After five cycles of the dry heating method, all three FFR models pass both fit and filtration tests, showing no degradation. For the autoclave tests we consider the 3M 1870, and the 3M 8210+. We find significant degradation of the FFRs following the 121 °C autoclave cycles. The molded mask tested (3M 8210+) failed fit testing after just 1 cycle in the autoclave. The pleated (3M 1870) mask passed fit testing for 5 cycles, but failed filtration testing. The 95 °C dry heat cycle is scalable to over a thousand masks per day in a hospital setting, and is above the temperature which has been shown to achieve the requisite 3 log kill of SARS-CoV-2[1], making it a promising method for N95 decontamination and reuse.</p> | |
Přístup | 12. 4. 2021 15:11:12 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.05.29.20114199 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), CC BY-NC 4.0, as described at http://creativecommons.org/licenses/by-nc/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.05.29.20114199 | |
Select in Zotero | zotero://select/library/items/VSGMRRKU | |
Datum přidání | 12. 4. 2021 15:11:12 | |
Upraveno | 9. 7. 2021 9:11:13 |
Autor | Yi Xiang | |
---|---|---|
Autor | Qifa Song | |
Autor | Wenzhen Gu | |
Typ | Článek v časopise | |
Datum | 2020-05-30 | |
Abstrakt | Background The need for protective masks greatly exceeds their global supply during the current COVID-19 pandemic. Methods We optimized the temperature used in the dry heat pasteurization method to destroy pathogens and decontaminate masks while retaining their filtering capacity. Results The current study showed that dry heat at both 60°C and 70°C for 1 hour could successfully kill 6 species of respiratory bacteria and one fungi species, and inactivate the H1N1 indicator virus. After being heated at 70°C for 1, 2, and 3 hours, the N95 respirators and surgical face masks showed no changes in their shape and components. The filtering efficiency of bacterial aerosol for N95 respirators were 98%, 98%, and 97% after being heated for 1, 2, and 3 hour, respectively, all of which were over the 95% efficiency required and similar to the value before being heated (99%). The filtering efficiency for surgical face masks was 97%, 97%, and 96% for 1, 2, and 3 hours of heating, respectively, all of which were also similar to the value before being heated (97%). Conclusions This method can be used at home and can significantly resolve the current shortage of masks. | |
Přístup | 2. 7. 2020 9:38:43 | |
Publikace | American Journal of Infection Control | |
Jazyk | en | |
ISSN | 0196-6553 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | American Journal of Infection Control | |
DOI | 10.1016/j.ajic.2020.05.026 | |
Select in Zotero | zotero://select/library/items/QBUQ3FAP | |
Datum přidání | 2. 7. 2020 9:38:43 | |
Upraveno | 5. 3. 2021 10:58:02 |
Autor | Laurent Guillier | |
---|---|---|
Autor | Sandra Martin-Latil | |
Autor | Estelle Chaix | |
Autor | Anne Thebault | |
Autor | Nicole Pavio | |
Autor | Sophie Le Poder | |
Autor | Christophe Batejat | |
Autor | Fabrice Biot | |
Autor | Lionel Koch | |
Autor | Don Schaffner | |
Autor | Moez Sanaa | |
Typ | Článek v časopise | |
Datum | 2020-05-29 | |
Abstrakt | Temperature and relative humidity are major factors determining virus inactivation in the environment. This article reviews inactivation data of coronaviruses on surfaces and in liquids from published studies and develops secondary models to predict coronaviruses inactivation as a function of temperature and relative humidity. A total of 102 D-values (time to obtain a log10 reduction of virus infectivity), including values for SARS-CoV-2, were collected from 26 published studies. The values obtained from the different coronaviruses and studies were found to be generally consistent. Five different models were fitted to the global dataset of D-values. The most appropriate model considered temperature and relative humidity. A spreadsheet predicting the inactivation of coronaviruses and the associated uncertainty is presented and can be used to predict virus inactivation for untested temperatures, time points or new coronavirus strains. | |
Přístup | 3. 7. 2020 8:17:20 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.05.26.20114025 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.05.26.20114025 | |
Select in Zotero | zotero://select/library/items/S9X2CCKL | |
Datum přidání | 3. 7. 2020 8:17:20 | |
Upraveno | 5. 3. 2021 10:58:07 |
Autor | Simeon C. Daeschler | |
---|---|---|
Autor | Niclas Manson | |
Autor | Kariym Joachim | |
Autor | Alex W. H. Chin | |
Autor | Katelyn Chan | |
Autor | Paul Z. Chen | |
Autor | Kiana Tajdaran | |
Autor | Kaveh Mirmoeini | |
Autor | Jennifer J. Zhang | |
Autor | Jason T. Maynes | |
Autor | Michelle Science | |
Autor | Ali Darbandi | |
Autor | Derek Stephens | |
Autor | Leo L. M. Poon | |
Autor | Frank Gu | |
Autor | Gregory H. Borschel | |
Typ | Článek v časopise | |
Datum | 2020-05-27 | |
Abstrakt | Background: The unprecedented demand and consequent global shortage of N95 respirators during the COVID-19 pandemic have left frontline workers vulnerable to infection. To expand the supply, we validated a rapidly applicable low-cost decontamination protocol in compliance with US-regulatory guidelines to enable the safe reuse of personalized, disposable N95-respirators. Methods: Four common models of N95-respirators were disinfected for 60 minutes at °C either at 0% or 50% relative humidity (RH). Effective inactivation of SARS-CoV-2 and E. coli was evaluated in inoculated masks. The N95 filter integrity was examined with scanning electron microscopy. The protective function of disinfected N95 respirators was tested against US NIOSH standards for particle filtration efficiency, breathing resistance and respirator fit. Results: A single heat treatment inactivated SARS-CoV-2 (undetectable, detection limit: 100 TCID50/ml) and E. coli (0 colonies at 50%RH) in all four respirator models. Even N95-respirators that underwent ten dry heat, or up to five 50%RH-controlled decontamination cycles maintained their integrity and met US-governmental criteria for approval regarding fit (10x dry heat: 194.48 ± 15.5; 99%CI: 185.5 to 203.5; 5x at 50%RH: 198.2 ± 6.3; 99%CI: 189.4 to 207; n=23 each, pass value ≥ 100,), breathing resistance (10x dry heat: 68.0 ± 8.4 Pa, 99%CI: 61.8 to 74.1, n=16; 5x at 50%RH: 78.5 ± 19.7 Pa, 99%CI: 21 to 135.9, n=4, pass value ≤ 343.23Pa), and filtration levels (10x dry heat: 97.6 ± 1.1%, 99%CI: 96.8 to 98.4; n=16; 5x at 50%RH: 97.18 ± 0.49, 99%CI: 95.7 to 98.6, n=4, pass value ≥ 95 %). Interpretation: This rapid process enables large-scale decontamination of existing N95-respirators using commonly sourced equipment during the COVID-19 pandemic. | |
Přístup | 3. 7. 2020 8:40:56 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.05.25.20112615 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author's permission. | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.05.25.20112615 | |
Select in Zotero | zotero://select/library/items/9TGH3R5S | |
Datum přidání | 3. 7. 2020 8:40:56 | |
Upraveno | 5. 3. 2021 10:58:12 |
Autor | Chamteut Oh | |
---|---|---|
Autor | Elbashir Araud | |
Autor | Joseph V. Puthussery | |
Autor | Hertz Bai | |
Autor | Vishal Verma | |
Autor | Thanh H. Nguyen | |
Typ | Článek v časopise | |
Datum | 2020-05-14 | |
Abstrakt | A pandemic such as COVID-19 can cause a depletion of face respirators throughout the world, forcing temporal respirator reuse. In this research, dry heat was systematically evaluated by decontamination, filtration performance, and qualitative fit testing to help safe reuse of N95 (1860, 3M) respirators. As a result, the dry heat generated by a cooker (120°C, 50 min) was effective in inactivating >4.7 log viruses without deteriorating its intended functions. Therefore, we suggest the dry heat generated by a pressure cooker (such as rice cookers and instant pots) as a reliable and accessible decontamination method for the N95 face respirator reuse. | |
Přístup | 3. 7. 2020 8:21:45 | |
Jazyk | en | |
Extra | Publisher: ChemRxiv | |
Katalog knihovny | chemrxiv.org | |
DOI | 10.26434/chemrxiv.12290621.v1 | |
Select in Zotero | zotero://select/library/items/XGARNTH5 | |
Datum přidání | 3. 7. 2020 8:21:45 | |
Upraveno | 5. 3. 2021 10:58:18 |
Autor | Claire Butkus | |
---|---|---|
Autor | Alena Veigl | |
Typ | Zpráva | |
Datum | 2020-05-11 | |
Abstrakt | In all studies investigating temperature and humidity effects on COVID-19 and related coronaviruses, low air temperature AT and low relativehumidity RH favored the survival of both SARS-CoV-2 and related coronaviruses however, inactivation was more rapid at increasing RH levelsand at high AT 1-8. In each case, high temperature at high RH were found to have a synergistic effect on the inactivation of coronavirus viabilitywhile lower temperatures and low relative humidity support prolonged survival of viruses on contaminated surfaces 3. The results of these studiessuggest that RH has a greater effect on viral stabilityinactivation that AT 2. | |
Přístup | 15. 10. 2020 14:31:33 | |
URL | https://apps.dtic.mil/sti/citations/AD1108916 | |
Jazyk | en | |
Extra | Section: Technical Reports | |
Katalog knihovny | apps.dtic.mil | |
Select in Zotero | zotero://select/library/items/SF3UGKSI | |
Datum přidání | 15. 10. 2020 14:31:33 | |
Upraveno | 15. 10. 2020 14:31:33 |
Autor | Dominique Kang | |
---|---|---|
Autor | Clifford Ellgen | |
Typ | Článek v časopise | |
Datum | 2020-05-05 | |
Abstrakt | Air temperature and body temperature may influence COVID-19 disease severity and transmission rates. In vitro data indicate that SARS-CoV-2 loses infectivity at normal core body temperature (37°C); however, small reductions in temperature proximate to 37°C may result in substantially increased viral stability. If these results are representative of viral decay rates in vivo, then cooler temperatures in the body may enable more rapid viral growth. Breathing cool air—even as warm as 25°C—cools upper respiratory tract (URT) surfaces to several degrees below body temperature, and these lower temperatures may make the URT exceptionally conducive to SARS-CoV-2 replication. Increased URT viral load may enable more effective transmission. Additionally, because SARS-CoV-2 infection may frequently begin in the URT before spreading through the body, an increased rate of viral replication in the URT early in the disease course may result in more rapid progression of disease, potentially causing more severe adverse outcomes. Core body temperature may also be a factor in disease severity, as lower core body temperatures may enable more rapid viral growth. The significance of air temperature and body temperature to disease severity and transmission rates may inform preventative measures and post-exposure prophylaxis treatments for COVID-19. | |
Přístup | 2. 7. 2020 14:42:22 | |
Publikace | MDPI AG, preprints | |
Jazyk | English | |
Práva | © 2020. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. | |
Extra | Place: Basel, Switzerland, Basel Publisher: MDPI AG Section: REVIEW | |
DOI | 10.20944/preprints202005.0070.v1 | |
Select in Zotero | zotero://select/library/items/27HH3D2Y | |
Datum přidání | 2. 7. 2020 10:56:25 | |
Upraveno | 5. 3. 2021 10:50:52 |
Autor | Tony T. Wang | |
---|---|---|
Autor | Christopher Z. Lien | |
Autor | Shufeng Liu | |
Autor | Prabhuanand Selvaraj | |
Typ | Článek v časopise | |
Datum | 2020-05-05 | |
Abstrakt | Abstract In this study, we aimed to evaluate the stability of SARS-CoV-2 under four different heat conditions (37, 42, 56, 60 °C) and report that the virus is stable at 37 °C for at least 24 hours. Heating at 56 °C for 30 minutes, however, effectively inactivated the virus while preserved the stability of viral RNA in both human sera and sputum samples. These findings provide critical information regarding the biology of the virus as well as a practical way to inactivate infectious virus that is potentially found in clinical specimens. | |
Přístup | 12. 4. 2021 15:15:51 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.04.29.20085498 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.04.29.20085498 | |
Select in Zotero | zotero://select/library/items/SFQ8PZDQ | |
Datum přidání | 12. 4. 2021 15:15:51 | |
Upraveno | 9. 7. 2021 9:11:37 |
Autor | Te Faye Yap | |
---|---|---|
Autor | Zhen Liu | |
Autor | Rachel A. Shveda | |
Autor | Daniel Preston | |
Typ | Článek v časopise | |
Datum | 2020-05-04 | |
Abstrakt | The COVID-19 pandemic has stressed healthcare systems and supply lines, forcing medical doctors to risk infection by decontaminating and reusing medical personal protective equipment intended only for a single use. The uncertain future of the pandemic is compounded by limited data on the ability of the responsible virus, SARS-CoV-2, to survive across various climates, preventing epidemiologists from accurately modeling its spread. However, a detailed thermodynamic analysis of experimental data on the inactivation of SARS-CoV-2 and related coronaviruses can enable a fundamental understanding of their thermal degradation that will help mitigate the COVID-19 pandemic and future outbreaks. This paper introduces a thermodynamic model that synthesizes existing data into an analytical framework built on first principles, including the Arrhenius equation and the rate law, to accurately predict the temperature-dependent inactivation of coronaviruses. The model provides much-needed thermal sterilization guidelines for personal protective equipment, including masks, and will also allow epidemiologists to incorporate the lifetime of SARS-CoV-2 as a continuous function of environmental temperature into models forecasting the spread of coronaviruses across different climates and seasons. | |
Přístup | 25. 5. 2020 15:54:36 | |
Jazyk | en | |
Extra | Publisher: ChemRxiv | |
Katalog knihovny | chemrxiv.org | |
DOI | 10.26434/chemrxiv.12152970.v2 | |
Select in Zotero | zotero://select/library/items/G6GXDJYT | |
Datum přidání | 25. 5. 2020 15:54:36 | |
Upraveno | 5. 3. 2021 10:58:39 |
Autor | Christophe Batéjat | |
---|---|---|
Autor | Quentin Grassin | |
Autor | Jean-Claude Manuguerra | |
Autor | India Leclercq | |
Typ | Článek v časopise | |
Datum | 2020-05-01 | |
Abstrakt | Supernatants of cells infected with SARS-CoV-2, nasopharyngeal and sera samples containing SARS-CoV-2 were submitted to heat inactivation for various periods of time, ranging from 30 seconds to 60 minutes. Our results showed that SARS-CoV-2 could be inactivated in less than 30 minutes, 15 minutes and 3 minutes at 56 C, 65 C and 95 C respectively. These data could help laboratory workers to improve their protocols with handling of the virus in biosafety conditions. | |
Přístup | 25. 5. 2020 16:04:47 | |
Publikace | bioRxiv | |
Jazyk | en | |
Rozsah | 2020.05.01.067769 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Section: New Results | |
Katalog knihovny | www.biorxiv.org | |
DOI | 10.1101/2020.05.01.067769 | |
Select in Zotero | zotero://select/library/items/N4J49A56 | |
Datum přidání | 25. 5. 2020 16:04:47 | |
Upraveno | 5. 3. 2021 10:58:45 |
Autor | Shi-Yan Ren | |
---|---|---|
Autor | Wen-Biao Wang | |
Autor | Ya-Guang Hao | |
Autor | Hao-Ran Zhang | |
Autor | Zhi-Chao Wang | |
Autor | Ye-Lin Chen | |
Autor | Rong-Ding Gao | |
Typ | Článek v časopise | |
Datum | 2020-04-26 | |
Abstrakt | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly contagious virus that can transmit through respiratory droplets, aerosols, or contacts. Frequent touching of contaminated surfaces in public areas is therefore a potential route of SARS-CoV-2 transmission. The inanimate surfaces have often been described as a source of nosocomial infections. However, summaries on the transmissibility of coronaviruses from contaminated surfaces to induce the coronavirus disease 2019 are rare at present. This review aims to summarize data on the persistence of different coronaviruses on inanimate surfaces. The literature was systematically searched on Medline without language restrictions. All reports with experimental evidence on the duration persistence of coronaviruses on any type of surface were included. Most viruses from the respiratory tract, such as coronaviruses, influenza, SARS-CoV, or rhinovirus, can persist on surfaces for a few days. Persistence time on inanimate surfaces varied from minutes to up to one month, depending on the environmental conditions. SARS-CoV-2 can be sustained in air in closed unventilated buses for at least 30 min without losing infectivity. The most common coronaviruses may well survive or persist on surfaces for up to one month. Viruses in respiratory or fecal specimens can maintain infectivity for quite a long time at room temperature. Absorbent materials like cotton are safer than unabsorbent materials for protection from virus infection. The risk of transmission via touching contaminated paper is low. Preventive strategies such as washing hands and wearing masks are critical to the control of coronavirus disease 2019. | |
Přístup | 25. 5. 2020 16:07:58 | |
Publikace | World Journal of Clinical Cases | |
Ročník | 8 | |
Číslo | 8 | |
Rozsah | 1391-1399 | |
Extra | PMID: 32368532 PMCID: PMC7190947 | |
ISSN | 2307-8960 | |
Katalog knihovny | PubMed Central | |
Zkrácený název časopisu | World J Clin Cases | |
DOI | 10.12998/wjcc.v8.i8.1391 | |
Select in Zotero | zotero://select/library/items/JCBL3WZE | |
Datum přidání | 25. 5. 2020 16:07:58 | |
Upraveno | 5. 3. 2021 10:58:51 |
Autor | Daniel F. Li | |
---|---|---|
Autor | Jennifer L. Cadnum | |
Autor | Sarah N. Redmond | |
Autor | Lucas D. Jones | |
Autor | Curtis J. Donskey | |
Typ | Článek v časopise | |
Datum | 2020-04-22 | |
Abstrakt | In the setting of the coronavirus disease 2019 (COVID-19) pandemic, healthcare facilities have been forced to adopt strategies to extend or reuse personal protective equipment (PPE) such as N95 filtering facepiece respirators and surgical face masks. Cloth face masks worn in public settings where social distancing cannot be maintained are also typically reused multiple times between laundering. A variety of strategies for decontamination of PPE are under investigation, including use of ultraviolet light and hydrogen peroxide vapor. | |
Přístup | 19. 5. 2020 8:40:32 | |
Publikace | American Journal of Infection Control | |
Krátký název | It's Not the Heat, It's the Humidity | |
Jazyk | English | |
Extra | Publisher: Elsevier PMID: 32334003 | |
ISSN | 1527-3296 | |
Katalog knihovny | www.ajicjournal.org | |
Zkrácený název časopisu | American Journal of Infection Control | |
DOI | 10.1016/j.ajic.2020.04.012 | |
Select in Zotero | zotero://select/library/items/5XL9XISV | |
Datum přidání | 19. 5. 2020 8:40:32 | |
Upraveno | 5. 3. 2021 10:59:03 |
Autor | Travis Massey | |
---|---|---|
Autor | Monica Borucki | |
Autor | Sam Paik | |
Autor | Kyle Fuhrer | |
Autor | Mihail Bora | |
Autor | Staci Kane | |
Autor | Razi Haque | |
Autor | Salmaan H. Baxamusa | |
Typ | Článek v časopise | |
Datum | 2020-04-22 | |
Abstrakt | Re-use of filtering facepiece respirators (FFRs, commonly referred to as N95s) normally meant for single use only is becoming common in healthcare facilities due to shortages caused by the COVID19 pandemic. Here we report that mouse hepatitis virus (MHV) initially seeded on FFR filter material is inactivated (6 log reduction as measured by 50% tissue culture infective dose (TCID50)) after dry heating at 75 °C for 30 minutes. We also find that the quantitative fit of FFRs after heat treatment at this temperature, under dry conditions or at 90% relative humidity, is not affected by single or ten heating cycles. Previous studies have reported that the filtration efficiency of FFR filters is not negatively impacted by these heating conditions. These results suggest that thermal inactivation of coronaviruses is a potentially rapid and widely deployable method to re-use N95 FFRs in emergency situations where re-using FFRs is a necessity and broad-spectrum sterilization is unavailable. However, we also found that a heat source that emits radiation (e.g., an exposed heating element) results in rapid qualitative degradation of the FFR. Finally, we discuss differences in the results reported here and other recent studies investing heat as a means to recycle FFRs and suggest that overall wear time and donning/doffing cycles are important factors that need to be considered. | |
Přístup | 25. 5. 2020 15:57:57 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.04.15.20065755 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution 4.0 International), CC BY 4.0, as described at http://creativecommons.org/licenses/by/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.04.15.20065755 | |
Select in Zotero | zotero://select/library/items/NBDM5GNA | |
Datum přidání | 25. 5. 2020 15:57:57 | |
Upraveno | 5. 3. 2021 10:58:56 |
Autor | Boris Pastorino | |
---|---|---|
Autor | Franck Touret | |
Autor | Magali Gilles | |
Autor | Xavier de Lamballerie | |
Autor | Remi N. Charrel | |
Typ | Článek v časopise | |
Datum | 2020-04-20 | |
Abstrakt | Clinical samples collected in COVID-19 patients are commonly manipulated in BSL-2 laboratories for diagnostic purpose. We used the French norm NF-EN-14476+A2 derived from the European standard EN-14885. To avoid the risk of exposure of laboratory workers, we showed that Triton-X100 must be added to guanidinium thiocyanate-lysis buffers to obtain a 6-log reduction of infectious virus. Although heating protocol consisting of 92C-15min was more effective rather than 56C-30min and 60C-60min to achieve 6-log reduction, it is not amenable for molecular detection on respiratory specimens because of important decrease of detectable RNA copies in the treated sample vs untreated sample. The 56C-30min and 60C-60min should be used for inactivation of serum / plasma samples for serology because of the 5log10 reduction of infectivity and low viral loads in blood specimens. | |
Přístup | 25. 5. 2020 16:19:13 | |
Publikace | bioRxiv | |
Jazyk | en | |
Rozsah | 2020.04.11.036855 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution 4.0 International), CC BY 4.0, as described at http://creativecommons.org/licenses/by/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Section: New Results | |
Katalog knihovny | www.biorxiv.org | |
DOI | 10.1101/2020.04.11.036855 | |
Select in Zotero | zotero://select/library/items/4ACR4FKU | |
Datum přidání | 25. 5. 2020 16:19:13 | |
Upraveno | 5. 3. 2021 10:59:12 |
Autor | Zhi-ping Sun | |
---|---|---|
Autor | Xia Cai | |
Autor | Chen-jian Gu | |
Autor | Rong Zhang | |
Autor | Wen-dong Han | |
Autor | Yun Qian | |
Autor | Yu-yan Wang | |
Autor | Wei Xu | |
Autor | Yang Wu | |
Autor | Xun-jia Cheng | |
Autor | Zheng-hong Yuan | |
Autor | You-hua Xie | |
Autor | Di Qu | |
Typ | Článek v časopise | |
Datum | 2020-04-14 | |
Abstrakt | COVID-19 has become a pandemic and is spreading fast worldwide. The COVID-19 virus is transmitted mainly through respiratory droplets and close contact. However, the fecal-oral transmission of the virus has not been ruled out and it is important to ascertain how acidic condition in the stomach affects the infectivity of the virus. Besides, it is unclear how stable the COVID-19 virus is under dry and wet conditions. In the present study, we have shown that the COVID-19 virus is extremely infectious as manifested by the infection of Vero-E6 cells by one PFU (Plaque Forming Unit) of the virus. We then investigated the stability of the COVID-19 virus in wet, dry and acidic (pH2.2) environments at room temperature. Results showed that the COVID-19 virus could survive for three days in wet and dry environments, but the dry condition is less favorable for the survival of the virus. Our study also demonstrated that the COVID-19 virus at a relative high titer (1.2 x 103 PFU) exhibits a certain degree of tolerance to acidic environment at least for 60 minutes. When the virus titer was ≤1.0 x 103 PFU, acid treatment (pH2.2) for 30 or 60 minute resulted in virus inactivation. It suggests that the virus at a high concentration may survive in the acidic environment of the stomach. The finding of the present study will contribute to the control of the spread of the COVID-19 virus. | |
Přístup | 3. 7. 2020 8:21:15 | |
Publikace | medRxiv | |
Jazyk | en | |
Rozsah | 2020.04.09.20058875 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Extra | Publisher: Cold Spring Harbor Laboratory Press | |
Katalog knihovny | www.medrxiv.org | |
DOI | 10.1101/2020.04.09.20058875 | |
Select in Zotero | zotero://select/library/items/KGIB8MQN | |
Datum přidání | 3. 7. 2020 8:21:15 | |
Upraveno | 5. 3. 2021 10:59:18 |
Autor | P. de Man | |
---|---|---|
Autor | B. van Straten | |
Autor | J. van den Dobbelsteen | |
Autor | A. van der Eijk | |
Autor | T. Horeman | |
Autor | H. Koeleman | |
Typ | Článek v časopise | |
Datum | 2020-04-08 | |
Abstrakt | The Covid-19 pandemic causes imminent shortages of face masks in hospitals globally. In preparation for that scarcity we performed a study to investigate the possibility of reprocessing disposable FFP2 face masks in order to verify their re-usability with a method that could be applied in practice using already available equipment. Therefore single use FFP2 masks (type 1862+3MTM) were sterilized with a 15-minute procedure at 121 ⁰C, using a dry sterilization process as well as with a regular steam process with the masks in sterilization/laminate bags. | |
Přístup | 25. 5. 2020 15:59:31 | |
Publikace | Journal of Hospital Infection | |
Jazyk | English | |
Ročník | 0 | |
Číslo | 0 | |
Extra | Publisher: Elsevier PMID: 32277964 | |
ISSN | 1532-2939 | |
Katalog knihovny | www.journalofhospitalinfection.com | |
Zkrácený název časopisu | Journal of Hospital Infection | |
DOI | 10.1016/j.jhin.2020.04.001 | |
Select in Zotero | zotero://select/library/items/N9PCRQZW | |
Datum přidání | 25. 5. 2020 15:59:31 | |
Upraveno | 5. 3. 2021 10:59:26 |
Autor | G. Kampf | |
---|---|---|
Autor | A. Voss | |
Autor | S. Scheithauer | |
Typ | Článek v časopise | |
Datum | 2020-03-31 | |
Abstrakt | The global spread of COVID-19 has resulted in a huge demand for personal protective equipment including face masks [1]. Even some hospitals face a substantial shortage of suitable face masks (e.g. FFP masks or N95 masks) resulting in an evaluation of various procedures to reprocess them for a limited re-use. Although they are classified as single use products the question was raised if a thermal disinfection may be effective to reduce coronaviruses. That is why published data were reviewed to find out which temperature and exposure time is necessary for inactivation of coronaviruses. | |
Přístup | 25. 5. 2020 15:50:19 | |
Publikace | Journal of Hospital Infection | |
Jazyk | English | |
Ročník | 0 | |
Číslo | 0 | |
Extra | Publisher: Elsevier PMID: 32243951 | |
ISSN | 1532-2939 | |
Katalog knihovny | www.journalofhospitalinfection.com | |
Zkrácený název časopisu | Journal of Hospital Infection | |
DOI | 10.1016/j.jhin.2020.03.025 | |
Select in Zotero | zotero://select/library/items/3HXTG7BQ | |
Datum přidání | 25. 5. 2020 15:50:19 | |
Upraveno | 5. 3. 2021 10:59:35 |
Autor | Qingxin Zhang | |
---|---|---|
Autor | Qingshun Zhao | |
Typ | Článek v časopise | |
Datum | 2020-02-22 | |
Abstrakt | 2019-Novel Coronavirus (2019-nCoV) is the pathogen of Corona Virus Disease 2019. Nucleic acid detection of 2019-nCoV is one of the key indicators for clinical diagnosis. However, the positive rate is only 30-50%. Currently, fluorescent quantitative RT-PCR technology is mainly used to detect 2019-nCoV. According to “The Laboratory Technical Guidelines for Detection 2019-nCoV (Fourth Edition)” issued by National Health and Commission of China and “The Experts’ Consensus on Nucleic Acid Detection of 2019-nCoV” released by Chinese Society of Laboratory Medicine, the human samples must be placed under 56°C or higher to inactivate the viruses in order to keep the inspectors from virus infection before the nucleic acids were isolated as the template of qRT-PCR. In this study, we demonstrated that the virus inactivation treatment disrupts its genome integrity seriously when using porcine epidemic diarrhea virus (vaccine), a kind of coronavirus, as a model. Our results showed that only 50.11% of the detectable viral templates left after the inactivation of 56 °C for 30 minutes and only 3.36% left after the inactivation of 92 °C for 5 minutes when the samples were preserved by Hank’s solution, one of an isotonic salt solutions currently suggested. However, the detectable templates of viral nucleic acids can be unchanged after the samples were incubated at 56 °C or higher if the samples were preserved with an optimized solution to protect the RNA from being disrupted. We therefore highly recommend to carry out systematic investigation on the impact of high temperature inactivation on the integrity of 2019-nCoV genome and develop a sample preservation solution to protect the detectable templates of 2019-nCoV nucleic acids from high temperature inactivation damage. | |
Přístup | 26. 5. 2020 6:46:53 | |
Publikace | bioRxiv | |
Jazyk | en | |
Rozsah | 2020.02.20.958785 | |
Práva | © 2020, Posted by Cold Spring Harbor Laboratory. The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author's permission. | |
Extra | Publisher: Cold Spring Harbor Laboratory Section: New Results | |
Katalog knihovny | www.biorxiv.org | |
DOI | 10.1101/2020.02.20.958785 | |
Select in Zotero | zotero://select/library/items/G8XMGJPD | |
Datum přidání | 26. 5. 2020 6:46:53 | |
Upraveno | 5. 3. 2021 10:59:42 |
Autor | Khan M. G. Mostofa | |
---|---|---|
Typ | Článek v časopise | |
Datum | 2020-02-11 | |
Abstrakt | A novel coronavirus, named 2019-nCoV, is responsible for current epidemic outbreaks in China and also other countries that cause acute pneumonia that was primarily linked to a seafood wholesale market in Wuhan, China. To control and prevent the existing epidemic outbreaks of coronavirus in China, a precise and easy disinfection/sterilization technique is important to disinfect/sterilize the mass-level peoples and their ambient environments (e.g. atmospheric air/aerosols). Among the disinfection techniques, control fire/flame (CF) could be applied. Large-size CF (LSCF) can be generated by using gas burner, gas cylinder that used in restaurant, coals, kerosene (petroleum), dried straws, dried woods) at entry and exit points of big gathering locations (e.g. hospital, airport, rail stations, seaports, apartments, streets/roads, etc. Additionally, small-size CF (SSCF) can be produced by using gas burner of kitchen, electric heater, kindle or waste papers at individual home and ward/room at hospital. Individual person can be soaked/immersed their hands and their belongings nearby CF for a moment, likely followed sterilization during quantification of the number of bacteria in experimental observation, that can rapidly sterilize the person perfectly. Correspondingly, the ambient air/aerosols moved towards CF due to water evaporation by fire that subsequently refresh all ambient air/aerosols by killing all viruses. Such CF could certainly reach the temperature higher than 56 degree C that could effectively disinfect/sterilize/kill the coronavirus. Therefore, CF could be effectively applied to control and prevent the epidemic outbreaks of coronavirus across China and worldwide. | |
Přístup | 3. 7. 2020 8:14:44 | |
URL | https://www.preprints.org/manuscript/202002.0141/v1 | |
Jazyk | en | |
Extra | Publisher: Preprints | |
Katalog knihovny | www.preprints.org | |
Select in Zotero | zotero://select/library/items/SG744E3W | |
Datum přidání | 3. 7. 2020 8:14:44 | |
Upraveno | 9. 7. 2021 12:13:31 |
Autor | India Leclercq | |
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Autor | Christophe Batéjat | |
Autor | Ana M. Burguière | |
Autor | Jean-Claude Manuguerra | |
Typ | Článek v časopise | |
Datum | 2014-07-29 | |
Abstrakt | The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10. Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV. | |
Přístup | 25. 5. 2020 15:00:03 | |
Publikace | Influenza and Other Respiratory Viruses | |
Jazyk | en | |
Ročník | 8 | |
Číslo | 5 | |
Rozsah | 585-586 | |
Práva | © 2014 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/irv.12261 | |
ISSN | 1750-2659 | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1111/irv.12261 | |
Select in Zotero | zotero://select/library/items/Z8KHZXUV | |
Datum přidání | 25. 5. 2020 15:00:03 | |
Upraveno | 5. 3. 2021 10:59:52 |
Autor | Chloe Geller | |
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Autor | Mihayl Varbanov | |
Autor | Raphaël E. Duval | |
Typ | Článek v časopise | |
Datum | 2012-11-12 | |
Abstrakt | The Coronaviridae family, an enveloped RNA virus family, and, more particularly, human coronaviruses (HCoV), were historically known to be responsible for a large portion of common colds and other upper respiratory tract infections. HCoV are now known to be involved in more serious respiratory diseases, i.e. bronchitis, bronchiolitis or pneumonia, especially in young children and neonates, elderly people and immunosuppressed patients. They have also been involved in nosocomial viral infections. In 2002-2003, the outbreak of severe acute respiratory syndrome (SARS), due to a newly discovered coronavirus, the SARS-associated coronavirus (SARS-CoV); led to a new awareness of the medical importance of the Coronaviridae family. This pathogen, responsible for an emerging disease in humans, with high risk of fatal outcome; underline the pressing need for new approaches to the management of the infection, and primarily to its prevention. Another interesting feature of coronaviruses is their potential environmental resistance, despite the accepted fragility of enveloped viruses. Indeed, several studies have described the ability of HCoVs (i.e. HCoV 229E, HCoV OC43 (also known as betacoronavirus 1), NL63, HKU1 or SARS-CoV) to survive in different environmental conditions (e.g. temperature and humidity), on different supports found in hospital settings such as aluminum, sterile sponges or latex surgical gloves or in biological fluids. Finally, taking into account the persisting lack of specific antiviral treatments (there is, in fact, no specific treatment available to fight coronaviruses infections), the Coronaviridae specificities (i.e. pathogenicity, potential environmental resistance) make them a challenging model for the development of efficient means of prevention, as an adapted antisepsis-disinfection, to prevent the environmental spread of such infective agents. This review will summarize current knowledge on the capacity of human coronaviruses to survive in the environment and the efficacy of well-known antiseptic-disinfectants against them, with particular focus on the development of new methodologies to evaluate the activity of new antiseptic-disinfectants on viruses. | |
Přístup | 25. 5. 2020 11:56:17 | |
Publikace | Viruses; Basel | |
Krátký název | Human Coronaviruses | |
Jazyk | English | |
Ročník | 4 | |
Číslo | 11 | |
Rozsah | 3044-3068 | |
Práva | Copyright MDPI AG 2012 | |
Extra | Num Pages: 3044-3068 Place: Basel, Switzerland, Basel Publisher: MDPI AG | |
Katalog knihovny | ProQuest | |
DOI | 10.3390/v4113044 | |
Select in Zotero | zotero://select/library/items/EFTJFVE4 | |
Datum přidání | 25. 5. 2020 11:56:17 | |
Upraveno | 5. 3. 2021 10:35:32 |
Autor | Anne-Marie Pagat | |
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Autor | Raphaelle Seux-Goepfert | |
Autor | Charles Lutsch | |
Autor | Valérie Lecouturier | |
Autor | Jean-François Saluzzo | |
Autor | Inca C. Kusters | |
Typ | Článek v časopise | |
Datum | 2007-06-01 | |
Abstrakt | When the first wave of the SARS epidemic seemed to have reached its conclusion, it was completely unclear how the spread of the virus would evolve. Taking into account all the uncertainties and anticipating the worst-case scenario, many laboratories and vaccine manufacturers started working on a vaccine approach against SARS infection. The results presented here describe the evaluation of decontamination practices performed within the framework of a SARS-Coronavirus (SARS-CoV) vaccine development project., We show that it takes 45 days at room temperature to fully inactivate the human SARS-CoV, whereas an enveloped virus such as the rabies virus, when treated in a similar way, is totally inactivated in three days. Moreover, the SARS-CoV is very resistant to alkaline treatment and, even more surprisingly, formaldehyde fumigation is not efficacious on the dried virus (under the conditions tested). Only heat (autoclave) and hypochlorite chloride treatments are efficacious treatments for the decontamination of SARS-CoV. | |
Přístup | 25. 5. 2020 13:06:34 | |
Publikace | Applied Biosafety | |
Jazyk | en | |
Ročník | 12 | |
Číslo | 2 | |
Rozsah | 100-108 | |
Extra | Publisher: SAGE Publications Inc | |
ISSN | 1535-6760 | |
Katalog knihovny | SAGE Journals | |
Zkrácený název časopisu | Appl Biosaf. | |
DOI | 10.1177/153567600701200206 | |
Select in Zotero | zotero://select/library/items/RAYGK7KS | |
Datum přidání | 25. 5. 2020 13:06:34 | |
Upraveno | 5. 3. 2021 10:35:41 |
Autor | H. F. Rabenau | |
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Autor | J. Cinatl | |
Autor | B. Morgenstern | |
Autor | G. Bauer | |
Autor | W. Preiser | |
Autor | H. W. Doerr | |
Typ | Článek v časopise | |
Datum | 2005-01 | |
Abstrakt | The SARS-coronavirus (SARS-CoV) is a newly emerged, highly pathogenic agent that caused over 8,000 human infections with nearly 800 deaths between November 2002 and September 2003. While direct person-to-person transmission via respiratory droplets accounted for most cases, other modes have not been ruled out. Faecal shedding is common and prolonged and has caused an outbreak in Hong Kong. We studied the stability of SARS-CoV under different conditions, both in suspension and dried on surfaces, in comparison with other human-pathogenic viruses, including human coronavirus HCoV-229E. In suspension, HCoV-229E gradually lost its infectivity completely while SARS-CoV retained its infectivity for up to 9 days; in the dried state, survival times were 24 h versus 6 days. Thermal inactivation at 56 C was highly effective in the absence of protein, reducing the virus titre to below detectability; however, the addition of 20% protein exerted a protective effect resulting in residual infectivity. If protein-containing solutions are to be inactivated, heat treatment at 60 C for at least 30 min must be used. Different fixation procedures, e.g. for the preparation of immunofluorescence slides, as well as chemical means of virus inactivation commonly used in hospital and laboratory settings were generally found to be effective. Our investigations confirm that it is possible to care for SARS patients and to conduct laboratory scientific studies on SARS-CoV safely. Nevertheless, the agents tenacity is considerably higher than that of HCoV-229E, and should SARS re-emerge, increased efforts need to be devoted to questions of environmental hygiene. | |
Přístup | 25. 5. 2020 11:51:35 | |
Publikace | Medical Microbiology and Immunology; Berlin | |
Jazyk | English | |
Ročník | 194 | |
Číslo | 1-2 | |
Rozsah | 1-6 | |
Práva | Copyright Springer-Verlag 2005 | |
Extra | Num Pages: 1-6 Place: Berlin, Netherlands, Berlin Publisher: Springer Nature B.V. | |
ISSN | 0300-8584 | |
Katalog knihovny | ProQuest | |
DOI | 10.1007/s00430-004-0219-0 | |
Select in Zotero | zotero://select/library/items/9C498RR6 | |
Datum přidání | 25. 5. 2020 11:51:35 | |
Upraveno | 5. 3. 2021 11:00:11 |
Autor | M. Yunoki | |
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Autor | T. Urayama | |
Autor | I. Yamamoto | |
Autor | S. Abe | |
Autor | K. Ikuta | |
Typ | Článek v časopise | |
Datum | 2004-12-07 | |
Abstrakt | Background and Objectives Various measures to inactivate/remove viruses have been implemented for manufacturing plasma-derived products. Here, we examined the heat inactivation ability of an agent of the severe acute respiratory syndrome (SARS), SARS coronavirus (CoV). Materials and Methods The Frankfurt-1 strain of SARS-CoV was incorporated in manufacturing processes of several products by using samples collected immediately before liquid heat treatment at 60 °C. Results SARS-CoV was easily inactivated by this treatment for 60 min in all in-process samples. However, the different composition of the tested samples affected the heat sensitivity of the virus strain: the infectivity of the virus in Antithrombin III preparation still remained after heating for 30 min at 60 °C. Conclusion If by rare chance SARS-CoV contaminates source plasma, there should be no or only minor risk of this virus infection, due to sufficient inactivation by the 60 °C 10 h liquid heating step, although we must pay attention to the composition used for blood product preparation. | |
Přístup | 25. 5. 2020 15:11:25 | |
Publikace | Vox Sanguinis | |
Jazyk | en | |
Ročník | 87 | |
Číslo | 4 | |
Rozsah | 302-303 | |
Extra | _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1423-0410.2004.00577.x | |
ISSN | 1423-0410 | |
Katalog knihovny | Wiley Online Library | |
DOI | 10.1111/j.1423-0410.2004.00577.x | |
Select in Zotero | zotero://select/library/items/ZJJKV5KQ | |
Datum přidání | 25. 5. 2020 15:11:25 | |
Upraveno | 5. 3. 2021 11:00:19 |
Autor | Miriam E. R. Darnell | |
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Autor | Kanta Subbarao | |
Autor | Stephen M. Feinstone | |
Autor | Deborah R. Taylor | |
Typ | Článek v časopise | |
Datum | 2004-10-01 | |
Abstrakt | Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254nm, heat treatment of 65°C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels. | |
Přístup | 25. 5. 2020 13:34:49 | |
Publikace | Journal of Virological Methods | |
Jazyk | en | |
Ročník | 121 | |
Číslo | 1 | |
Rozsah | 85-91 | |
ISSN | 0166-0934 | |
Katalog knihovny | ScienceDirect | |
Zkrácený název časopisu | Journal of Virological Methods | |
DOI | 10.1016/j.jviromet.2004.06.006 | |
Select in Zotero | zotero://select/library/items/LN3M9ULZ | |
Datum přidání | 25. 5. 2020 13:34:49 | |
Upraveno | 5. 3. 2021 10:35:52 |
Autor | Alain Lamarre | |
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Autor | Pierre J. Talbot | |
Typ | Článek v časopise | |
Datum | 1989-10-01 | |
Abstrakt | The stability of human coronavirus 229E infectivity was maximum at pH 6.0 when incubated at either 4 or 33 °C. However, the influence of pH was more pronounced at 33 °C. Viral infectivity was completely lost after a 14-day incubation period at 22, 33, or 37 °C but remained relatively constant at 4 °C for the same length of time. Finally, the infectious titer did not show any significant reduction when subjected to 25 cycles of thawing and freezing. These studies will contribute to optimize virus growth and storage conditions, which will facilitate the molecular characterization of this important pathogen.Key words: coronavirus, pH, temperature, infectivity, human coronavirus. | |
Přístup | 26. 5. 2020 6:49:15 | |
Publikace | Canadian Journal of Microbiology | |
Ročník | 35 | |
Číslo | 10 | |
Rozsah | 972-974 | |
Extra | Publisher: NRC Research Press | |
ISSN | 0008-4166 | |
Katalog knihovny | NRC Research Press | |
Zkrácený název časopisu | Can. J. Microbiol. | |
DOI | 10.1139/m89-160 | |
Select in Zotero | zotero://select/library/items/8FST9PIF | |
Datum přidání | 26. 5. 2020 6:49:15 | |
Upraveno | 5. 3. 2021 10:36:05 |