Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application

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Show simple item record Padil, Vinod Vellora Thekkae Černík, Miroslav 2016-05-24 2016-05-24 2013
dc.identifier.issn 1178-2013
dc.description.abstract Background: Copper oxide (CuO) nanoparticles have attracted huge attention due to catalytic, electric, optical, photonic, textile, nanofluid, and antibacterial activity depending on the size, shape, and neighboring medium. In the present paper, we synthesized CuO nanoparticles using gum karaya, a natural nontoxic hydrocolloid, by green technology and explored its potential antibacterial application. Methods: The CuO nanoparticles were synthesized by a colloid-thermal synthesis process. The mixture contained various concentrations of CuCl2 center dot 2H(2)O (1 mM, 2 mM, and 3 mM) and gum karaya (10 mg/mL) and was kept at 75 degrees C at 250 rpm for 1 hour in an orbital shaker. The synthesized CuO was purified and dried to obtain different sizes of the CuO nanoparticles. The well diffusion method was used to study the antibacterial activity of the synthesized CuO nanoparticles. The zone of inhibition, minimum inhibitory concentration, and minimum bactericidal concentration were determined by the broth microdilution method recommended by the Clinical and Laboratory Standards Institute. Results: Scanning electron microscopy analysis showed CuO nanoparticles evenly distributed on the surface of the gum matrix. X-ray diffraction of the synthesized nanoparticles indicates the formation of single-phase CuO with a monoclinic structure. The Fourier transform infrared spectroscopy peak at 525 cm(-1) should be a stretching of CuO, which matches up to the B-2u mode. The peaks at 525 cm(-1) and 580 cm(-1) indicated the formation of CuO nanostructure. Transmission electron microscope analyses revealed CuO nanoparticles of 4.8 +/- 1.6 nm, 5.5 +/- 2.5 nm, and 7.8 +/- 2.3 nm sizes were synthesized with various concentrations of CuCl2 center dot 2H(2)O (1 mM, 2 mM, and 3 mM). X-ray photoelectron spectroscopy profiles indicated that the O 1s and Cu 2p peak corresponding to the CuO nanoparticles were observed. The antibacterial activity of the synthesized nanoparticles was tested against Gram-negative and positive cultures. Conclusion: The formed CuO nanoparticles are small in size (4.8 +/- 1.6 nm), highly stable, and have significant antibacterial action on both the Gram classes of bacteria compared to larger sizes of synthesized CuO (7.8 +/- 2.3 nm) nanoparticles. The smaller size of the CuO nanoparticles (4.8 +/- 1.6 nm) was found to be yielding a maximum zone of inhibition compared to the larger size of synthesized CuO nanoparticles (7.8 +/- 2.3 nm). The results also indicate that increase in precursor concentration enhances an increase in particle size, as well as the morphology of synthesized CuO nanoparticles. en
dc.description.sponsorship Project OP VaVpI Center for Nanomaterials, Advanced Technologies and Innovation [CZ.1.05/2.1.00/01.0005]; Development of Research Teams of Research and Development Projects at the Technical University of Liberec [CZ.1.07/2.3.00/30.0024]
dc.format text
dc.language.iso en
dc.publisher Dove Medical Press Ltd
dc.publisher Technická Univerzita v Liberci cs
dc.publisher Technical university of Liberec, Czech Republic en
dc.relation.ispartof International Journal Of Nanomedicine en
dc.source j-scopus
dc.source j-wok
dc.subject gum karaya en
dc.subject CuO nanoparticles en
dc.subject XRD en
dc.subject FTIR en
dc.subject XPS en
dc.subject antibacterial activity en
dc.title Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application en
dc.type Article
dc.identifier.doi 10.2147/IJN.S40599
local.relation.volume 8
local.identifier.wok 317922700079
dc.identifier.scopus 2-s2.0-84874898033
local.faculty Institute for Nanomaterials, Advanced Technologies and Innovation
local.citation.spage 889
local.citation.epage 898
local.department Laboratory of Chemical Remediation Processes
local.access open
local.fulltext yes

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