Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

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Show simple item record Heidarrezaei, Mahshid Shokravi, Hoofar Huyop, Fahrul Koloor, Seyed Saeid Rahimian Petrů, Michal 2020-07-02T09:48:08Z 2020-07-02T09:48:08Z 2020-07-02
dc.description.abstract Halogenated compounds are an important class of environmental pollutants that are widely used in industrial chemicals such as solvents, herbicides, and pesticides. Many studies have been carried out to explore the biodegradation of these chemicals. Trichloroacetic acid (TCA) is one of the main halogenated compounds that are carcinogenic to humans and animals. The bacterium was isolated from the northern coastline of Johor Strait. In this study, the ability of strain MH2 to biodegrade TCA was evaluated by a growth experiment and dehalogenase enzyme assay. The growth profile of the isolated strain was examined. The doubling time for L. boronitolerans MH2 was found to be 32 h. The release of chloride ion in the degradation process was measured at 0.33 × 10−3 ± 0.03 mol∙L-1 after 96 h when the growth curve had reached its maximum within the late bacterial exponential phase. The results showed that the strain had a promising ability to degrade TCA by producing dehalogenase enzyme when cell-free extracts were prepared from growth on TCA as the sole carbon source with enzyme-specific activity, 1.1 ± 0.05 µmolCl-min−1∙mg−1 protein. Furthermore, the morphological, and biochemical aspects of the isolated bacterium were studied to identify and characterize the strain. The morphological observation of the isolated bacterium was seen to be a rod-shaped, Gram-positive, motile, heterotrophic, and spore-forming bacterium. The amplification of the 16S rRNA and gene analysis results indicated that the isolated bacterium had 98% similarity to Lysinibacillus boronitolerans. The morphological and biochemical tests supported the 16S rRNA gene amplification. To the best of the authors’ knowledge, this is the first reported case of this genus of bacteria to degrade this type of halogenated compound. cs
dc.format.extent 17 stran cs
dc.language.iso cs cs
dc.publisher MDPI
dc.relation.ispartof Applied Sciences 2020
dc.subject biodegradation cs
dc.subject dehalogenase-producing bacteria cs
dc.subject haloalkanoic acids cs
dc.subject trichloroacetic acid cs
dc.subject 16S rRNA cs
dc.subject Lysinibacillus cs
dc.title Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation cs
dc.identifier.doi 10.3390/app10134593
local.relation.volume 10
local.relation.issue 13
dc.identifier.orcid 0000-0002-1820-6379 Koloor, Seyed Saeid Rahimian
dc.identifier.orcid 0000-0002-7643-8450 Petrů, Michal
local.identifier.WebofScienceResearcherID G-6623-2013 Petrů, Michal

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